CN203587502U - Multi-parameter micro culture detection device - Google Patents
Multi-parameter micro culture detection device Download PDFInfo
- Publication number
- CN203587502U CN203587502U CN201320670641.9U CN201320670641U CN203587502U CN 203587502 U CN203587502 U CN 203587502U CN 201320670641 U CN201320670641 U CN 201320670641U CN 203587502 U CN203587502 U CN 203587502U
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- Prior art keywords
- pick
- unit
- detection device
- cultivated
- fluorescence detection
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- 238000001514 detection method Methods 0.000 title claims abstract description 45
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 32
- 239000002184 metal Substances 0.000 claims abstract description 26
- 238000010438 heat treatment Methods 0.000 claims abstract description 17
- 244000005700 microbiome Species 0.000 claims description 24
- 238000009413 insulation Methods 0.000 claims description 15
- 239000000523 sample Substances 0.000 claims description 13
- 239000007850 fluorescent dye Substances 0.000 claims description 9
- 238000005253 cladding Methods 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
Images
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a multi-parameter micro culture detection device which comprises a shell, wherein a fluorescence detection device and a culture detection device are arranged in the shell; the culture detection device comprises a high-temperature culture detection device and a low-temperature culture detection device which all comprise metal mounting plates, detection grooves and heating bands; the heating bands are attached to the bottom surfaces of the metal mounting plates; the detection grooves are formed in the top surfaces of the metal mounting plates; the detection grooves are separated to form multiple detection pools; a light source and a receiver are arranged in each detection pool; light emitted by the light sources passes through transparent sample bottles which are arranged in the detection pools and are filled with substances to be detected and then is received by the receivers; the fluorescence detection device is arranged between the high-temperature culture detection device and the low-temperature culture detection device. The multi-parameter micro culture detection device is simple in structure, easy to operate and convenient to use and can simultaneously detect four microbe parameters, photoelectric detection is utilized, a sample to be detected is not influenced, and the obtained detection result is accurate.
Description
Technical field
The utility model relates to a kind of pick-up unit, specifically, is a kind of cultivation pick-up unit that can simultaneously detect multiple-microorganism parameter.
Background technology
At present, how by experiment chamber method of microorganism detection.The method that coliform is detected in laboratory comprises multitube fermentation method, filter membrane method, Rapid paper strip method etc., the method of bacterial detection sum is to adopt colony counting method, the shortcoming of these class methods is test operation complexity, wastes time and energy, need to consume a large amount of experiment equipments, can not realize robotization and detect, and test result poor accuracy.
Utility model content
The utility model, for above-mentioned the deficiencies in the prior art, has designed a kind of multiparameter microorganism and has cultivated pick-up unit.
Multiparameter microorganism of the present utility model is cultivated pick-up unit, comprise shell, fluorescence detection device is installed in shell and cultivates pick-up unit, described cultivation pick-up unit is divided into high-temperature cultivation pick-up unit and low temperature is cultivated pick-up unit, all comprise metal mounting plate, detect groove and heating tape, heating tape is attached on the bottom surface of metal mounting plate, detecting groove is placed on the end face of metal mounting plate, detect groove and be all divided into multiple detection cells, light source and receiver are installed in each detection cell, the light that light source sends, through after being placed on the transparent sample bottle that is contained with material to be checked in detection cell, be received by the receiver, fluorescence detection device is arranged on high-temperature cultivation pick-up unit and low temperature is cultivated between pick-up unit.
Preferably, the bottom land of detection groove is arranged in the pickup groove of metal mounting plate setting.
Preferably, an insulation cladding is housed in shell, fluorescence detection device and cultivation pick-up unit are all arranged in insulation cladding, on shell, be provided with and can cover the insulation cover that detects the opening that groove and fluorescence detection device expose, high-temperature cultivation pick-up unit, low temperature cultivate pick-up unit and fluorescence detection device both sides are all provided with thermal insulation board.
Preferably, the metal mounting plate of high-temperature cultivation pick-up unit is the narrow I shape in wide centre, two ends.
Preferably, low temperature is cultivated pick-up unit and is provided with two groups of detection grooves, and the heating tape lower surface that low temperature is cultivated pick-up unit is equipped with heat sink.
Preferably, fluorescence detection device comprises fluorescence detection cell, ultraviolet source and fluorescent probe, rotary barrel is installed in fluorescence detection cell, lucifuge lid is installed on the top of rotary barrel, the sidewall of rotary barrel has and detects mouth, the sidewall of fluorescence detection cell is provided with two printing opacity mouths, and ultraviolet source and fluorescent probe are installed respectively on two printing opacity mouths, and two printing opacity mouths can align with detection mouthful simultaneously.
Preferably, on two printing opacity mouths are interior, optical filter is all installed, the printing opacity mouth edge corresponding with fluorescence detector is provided with lucifuge circle, between ultraviolet source and its corresponding printing opacity mouth, lucifuge plate is also installed.
The beneficial effects of the utility model are: it is simple that multiparameter microorganism is cultivated structure of the detecting device, and processing ease is easy to use, can detect 4 kinds of microbiological parameters simultaneously, utilizes Photoelectric Detection, does not affect sample to be tested, and the testing result obtaining is more accurate.
Cultivate the heating tape of the sheet of pick-up unit by homogeneous heating as thermal source, utilize metal mounting plate heat conduction, quick heating, homogeneous heating.The bottom land that detects groove is arranged in the pickup groove of metal mounting plate setting, has improved and has detected the area that groove contacts with metal mounting plate, has improved the programming rate of heat transfer efficiency and detection groove.
The metal mounting plate of high-temperature cultivation pick-up unit is the narrow I shape in wide centre, two ends, and the heat of metal mounting plate is concentrated, and has guaranteed the needs of high temperature.The heating tape lower surface that low temperature is cultivated pick-up unit is equipped with heat sink, and the area of its metal mounting plate is also greater than the metal mounting plate of high-temperature cultivation pick-up unit, when room temperature is cultivated the design temperature of pick-up unit higher than low temperature, can be initiatively its cooling, equipment can be used under various ambient conditions.
Three kinds of pick-up units that multiparameter microorganism is cultivated pick-up unit inside are arranged in insulation cladding, opening is covered with insulation cover, and all by thermal insulation board, separate between three kinds of pick-up units, kept the temperature constant in region separately, make the stable cultivation of sample to be tested.Fluorescence detection device is arranged on high-temperature cultivation pick-up unit and low temperature is cultivated between pick-up unit, has increased high-temperature cultivation pick-up unit and low temperature and has cultivated the distance between pick-up unit, has alleviated both are separated and keep the difficulty of temperature constant separately.
The detection mouth of fluorescence detection device rotary barrel is aimed at two printing opacity mouths when detecting, rotation while not detecting, make its sidewall block two printing opacity mouths, guarantee that fluorescence detector is all the time in lucifuge state, avoid causing damage, in the serviceable life of improving pick-up unit entirety, lucifuge circle and lucifuge plate have also further guaranteed the effect of lucifuge.
Accompanying drawing explanation
Accompanying drawing 1 is the structural drawing of multiparameter microorganism cultivation pick-up unit inside;
The structural drawing that accompanying drawing 2 is fluorescence detection device.
Embodiment
Multiparameter microorganism of the present utility model is cultivated pick-up unit, as shown in Figure 1, comprises shell, fluorescence detection device 1 is installed in shell and cultivates pick-up unit.
Cultivate pick-up unit and be divided into high-temperature cultivation pick-up unit 2 and low temperature cultivation pick-up unit 3, all comprise metal mounting plate 4, detect groove 5 and heating tape 6, heating tape 6 is attached on the bottom surface of metal mounting plate 4, detect groove 5 and be placed on the end face of metal mounting plate 4, detect the bottom land of groove 5 and be arranged in the pickup groove that metal mounting plate 4 arranges.
Detect groove 5 and be all divided into multiple detection cells 7, in each detection cell 7, light source and receiver are installed, the light that light source sends, is received by the receiver through after being placed on the transparent sample bottle that is contained with material to be checked in detection cell 7.
The metal mounting plate 4 of high-temperature cultivation pick-up unit 2 is the narrow I shape in wide centre, two ends.Low temperature is cultivated pick-up unit 3 and is provided with two groups of detection grooves 5, and heating tape 6 lower surfaces that low temperature is cultivated pick-up unit 3 are equipped with heat sink 10.
An insulation cladding 8 is housed in shell, fluorescence detection device 1 and cultivation pick-up unit are all arranged in insulation cladding 8, on shell, be provided with and can cover the insulation cover that detects the opening that groove 5 and fluorescence detection device 1 expose, high-temperature cultivation pick-up unit 2, low temperature cultivate pick-up unit 3 and fluorescence detection device 1 both sides are all provided with thermal insulation board 9.
Fluorescence detection device 1 is arranged on high-temperature cultivation pick-up unit 2 and low temperature is cultivated between pick-up unit 3.
Fluorescence detection device 1 comprises fluorescence detection cell 11, ultraviolet source 12 and fluorescent probe 13, rotary barrel 14 is installed in fluorescence detection cell 11, lucifuge lid 15 is installed on the top of rotary barrel 14, the sidewall of rotary barrel 14 has and detects mouth 16, the sidewall of fluorescence detection cell 11 is provided with two printing opacity mouths 17, ultraviolet source 12 and fluorescent probe 13 are installed respectively on two printing opacity mouths 17, and two printing opacity mouths 17 can align with detection mouth 16 simultaneously.On two printing opacity mouths 17 are interior, optical filter 18 is all installed, printing opacity mouth 17 edges corresponding with fluorescence detector 13 are provided with lucifuge circle 19, between ultraviolet source 12 and its corresponding printing opacity mouth 17, lucifuge plate 20 are also installed.
While detecting, microorganism to be measured is dropped into and is contained with in the sample plasma bottle of nutrient culture media, then sample plasma bottle is put into the corresponding detection cell 7 that detects groove 5.The heat-resisting coliform of the interior detection of detection groove 5 of high-temperature cultivation pick-up unit 2, low temperature is cultivated two interior total coli group and totals number of bacteria of detecting respectively of detection groove 5 of pick-up unit 3.
Microorganism is in sample plasma bottle, and nutrient culture media provides nutrition, and heating tape 6 transfers heat to sample plasma bottle by metal mounting plate 4 and detection groove 5, for microorganism existence provides temperature.
After microorganism has been cultivated, utilize the light source of detection cell 7 to send light to sample plasma bottle, light, through after sample plasma bottle, is received by the receiver, and multiparameter microorganism is cultivated pick-up unit just can obtain testing result according to the situation of the light of receiver reception.
While carrying out fluoroscopic examination, need to first rotate rotary barrel 14, the sidewall of rotary barrel 14 is blocked two printing opacity mouths 17, make ultraviolet source 12 and fluorescent probe 13 all in lucifuge state, just can open lucifuge lid 15, the sample plasma bottle that is contained with microorganism to be detected is put into rotary barrel 14, then cover lucifuge lid 15, rotate rotary barrel 14, the detection mouth 16 of rotary barrel 14 is aimed to two printing opacity mouths 17, the light that ultraviolet source 12 sends is successively by lucifuge plate 20, optical filter 18, be injected in rotary barrel 14, excite the microorganism to be detected in sample plasma bottle, make microorganism send fluorescence, fluorescence is successively by another optical filter 18, by fluorescent probe 13, received, the situation of the fluorescence that multiparameter microorganism cultivation pick-up unit just can receive according to fluorescent probe 13 obtains testing result.
Claims (7)
1. a multiparameter microorganism is cultivated pick-up unit, it is characterized in that, comprise shell, fluorescence detection device (1) is installed in shell and cultivates pick-up unit, described cultivation pick-up unit is divided into high-temperature cultivation pick-up unit (2) and low temperature is cultivated pick-up unit (3), all comprise metal mounting plate (4), detect groove (5) and heating tape (6), heating tape (6) is attached on the bottom surface of metal mounting plate (4), detecting groove (5) is placed on the end face of metal mounting plate (4), detect groove (5) and be all divided into multiple detection cells (7), each detection cell is provided with light source and receiver in (7), the light that light source sends, through after being placed on the transparent sample bottle that is contained with material to be checked in detection cell (7), be received by the receiver, fluorescence detection device (1) is arranged on high-temperature cultivation pick-up unit (2) and low temperature is cultivated between pick-up unit (3).
2. multiparameter microorganism according to claim 1 is cultivated pick-up unit, it is characterized in that, the bottom land that detects groove (5) is arranged in the pickup groove of metal mounting plate (4) setting.
3. multiparameter microorganism according to claim 1 is cultivated pick-up unit, it is characterized in that, an insulation cladding (8) is housed in shell, fluorescence detection device (1) and cultivation pick-up unit are all arranged in insulation cladding (8), on shell, be provided with and can cover the insulation cover that detects groove (5) and the opening that exposes of fluorescence detection device (1), high-temperature cultivation pick-up unit (2), low temperature cultivation pick-up unit (3) and fluorescence detection device (1) both sides are all provided with thermal insulation board (9).
4. multiparameter microorganism according to claim 1 is cultivated pick-up unit, it is characterized in that, the metal mounting plate (4) of high-temperature cultivation pick-up unit (2) is the narrow I shape in wide centre, two ends.
5. multiparameter microorganism according to claim 1 is cultivated pick-up unit, it is characterized in that, low temperature is cultivated pick-up unit (3) and is provided with two groups of detection grooves (5), and heating tape (6) lower surface that low temperature is cultivated pick-up unit (3) is equipped with heat sink (10).
6. multiparameter microorganism according to claim 1 is cultivated pick-up unit, it is characterized in that, fluorescence detection device (1) comprises fluorescence detection cell (11), ultraviolet source (12) and fluorescent probe (13), rotary barrel (14) is installed in fluorescence detection cell (11), lucifuge lid (15) is installed on the top of rotary barrel (14), the sidewall of rotary barrel (14) has and detects mouthful (16), the sidewall of fluorescence detection cell (11) is provided with two printing opacity mouths (17), ultraviolet source (12) and fluorescent probe (13) are installed respectively on two printing opacity mouths (17), two printing opacity mouths (17) can align with detection mouthful (16) simultaneously.
7. multiparameter microorganism according to claim 6 is cultivated pick-up unit, it is characterized in that, on in two printing opacity mouths (17), optical filter (18) is all installed, printing opacity mouth (17) edge corresponding with fluorescence detector (13) is provided with lucifuge circle (19), between ultraviolet source (12) and its corresponding printing opacity mouth (17), lucifuge plate (20) is also installed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320670641.9U CN203587502U (en) | 2013-10-28 | 2013-10-28 | Multi-parameter micro culture detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320670641.9U CN203587502U (en) | 2013-10-28 | 2013-10-28 | Multi-parameter micro culture detection device |
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| Publication Number | Publication Date |
|---|---|
| CN203587502U true CN203587502U (en) | 2014-05-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320670641.9U Expired - Lifetime CN203587502U (en) | 2013-10-28 | 2013-10-28 | Multi-parameter micro culture detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203587502U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107663504A (en) * | 2017-10-09 | 2018-02-06 | 宜昌喜旺食品有限公司 | A kind of equipment and detection method of Intelligent Measurement milk bacterial number |
| CN112881356A (en) * | 2021-01-18 | 2021-06-01 | 上海雄图生物科技有限公司 | High-flux fluorescence immunoassay quantitative POCT analysis device |
-
2013
- 2013-10-28 CN CN201320670641.9U patent/CN203587502U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107663504A (en) * | 2017-10-09 | 2018-02-06 | 宜昌喜旺食品有限公司 | A kind of equipment and detection method of Intelligent Measurement milk bacterial number |
| CN107663504B (en) * | 2017-10-09 | 2024-03-19 | 宜昌喜旺食品有限公司 | Device and method for intelligently detecting bacterial quantity of milk |
| CN112881356A (en) * | 2021-01-18 | 2021-06-01 | 上海雄图生物科技有限公司 | High-flux fluorescence immunoassay quantitative POCT analysis device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140507 |
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| CX01 | Expiry of patent term |