CN202089988U - Device for measuring bacterium concentration in situ - Google Patents
Device for measuring bacterium concentration in situ Download PDFInfo
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- CN202089988U CN202089988U CN 201120189706 CN201120189706U CN202089988U CN 202089988 U CN202089988 U CN 202089988U CN 201120189706 CN201120189706 CN 201120189706 CN 201120189706 U CN201120189706 U CN 201120189706U CN 202089988 U CN202089988 U CN 202089988U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a device for measuring bacterium concentration in situ, which comprises a liquid storage container and a cuvette which are communicated through a guide pipe. In the device disclosed by the utility model, as the liquid storage container and the cuvette are communicated through the guide pipe, when the bacterium concentration is measured, the bacterium liquid to be measured in the liquid storage container is poured into the cuvette through the guide pipe, then the cuvette is placed in a spectrophotometer, the liquid storage container is placed at one side to measure the OD (Outside Dimension) of the bacterium liquid at a position with a certain wavelength, and then the bacterium concentration can be measured in real time. The structure of the device can avoid the condition that in the prior art, when the bacterium concentration of the bacterium liquid is measured, various negative factors in the sampling process influence the real bacterium concentration. By adopting the structure of the utility model, the sampling is free, and the bacterium concentration measurement in situ can be carried out. The device disclosed by the utility model has the advantages that the volume of the bacterium liquid in bacterium culture can not be changed, no pollution is caused, the bacterium concentration can be measured in real time, and the like.
Description
Technical field
The utility model relates to a kind of device of in-site detecting bacterial concentration, relates in particular to a kind of device that is applicable to the in-site detecting bacterial concentration of the bacterial concentration that cultivation of The real time measure shaking table or small-sized fermentation are cultivated.
Background technology
The mensuration of bacterial concentration is regular work in the microbiological research test, and its measuring method commonly used has dilution plate counting process, weighting method, spectrophotometric turbidimetry, blood counting chamber counting process, electrical conductivity method etc.; The method that adopts at most in the present domestic laboratory is the spectrophotometric turbidimetry, and this method is that inoculum is taken a sample, and measures the OD value of bacterium liquid then on spectrophotometer, according to the OD value of bacterium liquid, calculates the concentration of bacterium liquid rapidly and accurately.Adopt this kind method in the process of sampling, usually can cause the pollution of original bacteria liquid and the volume minimizing etc. that makes original bacteria liquid, thereby the accuracy that causes bacterial concentration to be measured is affected; In addition, in the process of sampling, also can pollute surrounding environment.If the frequency of sampling and quantity are more for a long time, workload is strengthened, complex operation, and can not measure the concentration of bacterium real-time and accurately.
The utility model content
The purpose of this utility model is to provide a kind of device of in-site detecting bacterial concentration, with accurately, measures bacterial concentration efficiently.
To achieve these goals, the technical solution of the utility model has adopted a kind of device of in-site detecting bacterial concentration, comprises the storage liquid container, cuvette, and the storage liquid container is connected by conduit with cuvette.
Described storage liquid container is an Erlenmeyer flask, and the bottle wall of Erlenmeyer flask is provided with a liquid outlet, and liquid outlet is communicated with cuvette by conduit.
Described conduit is a polythene material.
Adopt device of the present utility model, the storage liquid container is connected by conduit with cuvette, when measuring bacterial concentration, to store in the bacterium liquid impouring cuvette to be measured in the liquid container by conduit, then cuvette is put into spectrophotometer, the storage liquid container is placed on a side, measures the OD value of bacterium liquid at certain wavelength place, can measure bacterial concentration in real time; The multiple unfavorable factor of having brought in the sampling process when structure of the present utility model has avoided existing method to measure bacterial concentration is to the influence of bacterium actual concentration, adopt structure of the present utility model to take a sample, carry out the in-site detecting bacterial concentration, has the volume that does not change bacterium liquid in the microbial culture, can not pollute and the real-time advantage of bacterial detection concentration, can be simply, fast, the real-time method of measuring bacterial concentration accurately.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
As shown in Figure 1, the device of in-site detecting bacterial concentration of the present utility model comprises storage liquid container 1, cuvette 4, and storage liquid container 1 is connected by conduit 3 with cuvette 4.Wherein, storage liquid container 1 is an Erlenmeyer flask, and the bottle wall of Erlenmeyer flask is provided with a liquid outlet 2, and liquid outlet 2 is communicated with cuvette 4 by conduit 3, and conduit 3 is a polythene material.
Using method is: before inoculated bacteria, allow the substratum of storing in the liquid container 1 flow in the cuvette 4 by conduit 3, at spectrophotometer adjusted zero point, to do to survey periodic contrast, in the process that shaking table is cultivated, conduit 3 is fixed on the storage liquid container 1, during mensuration, regularly take off storage liquid container 1 from shaking table, in nutrient solution impouring cuvette 4, and on spectrophotometer, measure the OD value, and calculate bacterial concentration, can be back to continuation cultivation in the storage liquid container 1 with measuring bacterium liquid rapidly after the mensuration.
Claims (3)
1. the device of an in-site detecting bacterial concentration is characterized in that: comprise the storage liquid container, and cuvette, the storage liquid container is connected by conduit with cuvette.
2. the device of in-site detecting bacterial concentration according to claim 1 is characterized in that: described storage liquid container is an Erlenmeyer flask, and the bottle wall of Erlenmeyer flask is provided with a liquid outlet, and liquid outlet is communicated with cuvette by conduit.
3. the device of in-site detecting bacterial concentration according to claim 1 and 2 is characterized in that: described conduit is a polythene material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120189706 CN202089988U (en) | 2011-05-31 | 2011-05-31 | Device for measuring bacterium concentration in situ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120189706 CN202089988U (en) | 2011-05-31 | 2011-05-31 | Device for measuring bacterium concentration in situ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202089988U true CN202089988U (en) | 2011-12-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120189706 Expired - Fee Related CN202089988U (en) | 2011-05-31 | 2011-05-31 | Device for measuring bacterium concentration in situ |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202089988U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630067A (en) * | 2015-02-02 | 2015-05-20 | 新奥科技发展有限公司 | Pollution preventing and treating method for microalga breeding |
| CN105572057A (en) * | 2016-01-05 | 2016-05-11 | 山西瑞亚力科技有限公司 | Manufacturing method of colorimetric card capable of rapidly measuring turbidity of microbe nutrient solution |
| CN109554294A (en) * | 2018-12-27 | 2019-04-02 | 佛山科学技术学院 | A kind of continuous record growth curve of bacteria device |
-
2011
- 2011-05-31 CN CN 201120189706 patent/CN202089988U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630067A (en) * | 2015-02-02 | 2015-05-20 | 新奥科技发展有限公司 | Pollution preventing and treating method for microalga breeding |
| CN105572057A (en) * | 2016-01-05 | 2016-05-11 | 山西瑞亚力科技有限公司 | Manufacturing method of colorimetric card capable of rapidly measuring turbidity of microbe nutrient solution |
| CN109554294A (en) * | 2018-12-27 | 2019-04-02 | 佛山科学技术学院 | A kind of continuous record growth curve of bacteria device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111228 Termination date: 20120531 |