CN108004140A - A kind of bioreactor population parameter detecting system and its measurement control method - Google Patents
A kind of bioreactor population parameter detecting system and its measurement control method Download PDFInfo
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Abstract
The present invention provides a kind of bioreactor population parameter detecting system and its measurement control method, reactor control system is mainly made of embedded microcontroller and Field Programmable Gate Array Devices, temperature adjustment module, surging adjustment module, liquid level adjustment module, flow-rate adjustment module, gsm module, detection control module etc.；It can realize the detection of the population parameters such as bioreactor temperature, pressure, flow, liquid level, oxygen concentration, gas concentration lwevel and weight and automatically control, all kinds of supplemental characteristics for detecting and collecting carry out calculating processing, analyze the oxygen uptake rate for obtaining bioprocesses, carbon dioxide production rate and than growth rate etc., it is possible to achieve the optimization of bioprocesses.
Description
Technical field
The invention belongs to technical field of automatic control, is mainly used in reaction process in the fields such as pharmacy, food, chemical industry
The on-line checking of middle response parameter and automatically control, realize the optimization of reaction process, specifically relate to a kind of bioreactor population parameter
Detecting system and its measurement control method.
Background technology
Bioreactor is the device system for carrying out biochemical reaction in vitro using biological function possessed by enzyme or organism
System, it is a kind of biological function analog machine (container), such as fermentation tank, enzyme or cell reactor, in food and fermentation (such as wine
Class, amino acid, organic acid), medical (antibiotic, interferon), environment (biodegradation), bioenergy (cellulosic ethanol, oil-producing
Microalgae, biogas) etc. production and scientific research field extensive use.
Since bioprocesses are the complex processes that have vital signs cell metabolism, active somatic cell is in the reactor
Physiological status regulation and control are reactor reinforcing, process optimization and the core of amplification, know life of the cell in reactor environment in time
Metabolic characteristic is managed, it is to realize bioprocesses optimization and the premise that bioreactor is strengthened to design rational reactor assembly；
In bioprocesses optimizing research, the change of reactor operating condition can influence intracellular microcosmic metabolism, corresponding thereto
Reactor aspect macroscopic view metabolic condition can also change；By the lab scale reactor cell physiological metabolism state optimized big
Reappeared in type reactor, understand and grasp different reactor designs, operating condition flows down the situation of change of field characteristic, with reference to grand
See physiological property change to be analyzed, maintain the Effec-tive Function of bioreactor to need to control the bioprocess in reactor
System and optimization, so as to obtain the biological transformation ratio of maximum and minimum reaction cost, the basis of control and optimization is exactly to reaction
Various response parameters are detected and automatically control in device.
The detection mode of each parameter bioreactor has two kinds of offline inspection and on-line checking in reaction process.Offline inspection
It is by manual sampling, sample is then sent to laboratory or instrument room, is measured by special instrument.On-line checking is inspection
Survey instrument directly link together with reactor or detection sensor probe intercalation reaction device in be detected, sample and detected
Journey can continuously, it is rapid, accurate complete, can in time, the dynamic change of accurate, continuous reflection reactor biochemical process, be conducive to
The optimization and control of bioprocesses.
In the detection of bioreactor population parameter, except the temperature of conventional detection, DO, pH value, rotating speed etc., it is provided with
The detection of special parameter：Tail gas oxygen and carbon dioxide concentration is detected, for calculating the oxygen uptake rate of whole bioprocesses
(OUR) and carbon dioxide generating rate (CER)；At the same time in order to calculate above-mentioned two physiological metabolism parameter, also need to configure accurate survey
The mass flowmenter of determining air mass flow, the pressure transmitter for being directly installed on reactor head, reactor weighing system (industry rule
Mould is differential pressure transmitter) metered volume and the feed supplement electricity scale system to the detection of metabolin mass flow etc..
The various features of binding reactor, realize all kinds of procedure parameters of bioreactor online mass data detection with adopting
Collection, fusion various procedures theory and control theory, row correlation computations and analysis are driven into reference to Biological Knowledge, to these parameters,
Multiple dimensioned industrial analysis is carried out to reaction process and optimization operates, realization is possible to and changes in reactor dimensions, or even gene
The optimization of reaction process is realized during dimensional variation and is automatically controlled.
The content of the invention
The present invention is intended to provide a kind of bioreactor population parameter detecting system and its measurement control method, preferably solve
Each procedure parameter online data detection of bioreactor is calculated with collection, data and processing, changes in reactor dimensions, or even base
Because realizing the optimization of reaction process during dimensional variation and automatically controlling.To achieve these goals, the present invention uses following technology
Scheme：
A kind of bioreactor population parameter detecting system, is mainly used for the bioreactor population parameter detection shown in Fig. 1；It is biological anti-
Device population parameter detecting system composition is answered mainly to have：Bioreactor, agitator motor and frequency converter, electronic weighing instrument, liquid level become
Send device, pH value measuring instrument, dissolved oxygen sensing instrument, temperature transmitter, control system, O2Concentration meter, CO2Concentration meter, pressure sensing
Device, air valve, air flow meter, cooling water valve, cooling water flow meter, weighing instrument, inlet valve and baiting valve form.
Control system described in a kind of bioreactor population parameter detecting system, as shown in Fig. 2, mainly by embedded micro-control
Device and field programmable gate array (FPGA) device processed, measurement and control of temperature module, pressure testing and controlling module, flow measuring and controlling module, concentration
Control module, pH value control module, weight control module, gsm module, local and network PC, LCD control, various sensors, beat
Print machine and detection control module composition；Mainly by embedded microcontroller and field programmable gate array (FPGA) device, temperature
Adjustment module, vacuum adjustment module, gsm module, local and network PC, LCD control, various sensors, printer and detection,
Control module forms, as shown in Fig. 2, realizing the functions such as the parameter detecting of bioreactor, live display, field control, at the same time
Realize the automated control of bioreactor temperature, pressure, flow, concentration and liquid level；Programmable gate array (FPGA) device
Part, there is provided a large amount of ram spaces preserve intermediate parameters and computation of table lookup, there is provided substantial amounts of general purpose I/O Interface, for input and output
Module and the accurate PWM modulation function of quick response；The detection measurement module, is mainly adjusted and is adopted by sensor, signal
The composition such as collection, realizes the measurement of bioreactor temperature, pressure, flow, concentration and liquid level and the state of each critical piece；Institute
State control module to be mainly made of driving and executing agency, realize the driving and control of air valve, water valve, motor, frequency converter etc.；
The LCD touch screen, IDE hard disks, printer co-ordination are to realize the real-time collection of bioreactor population parameter, display, defeated
Go out and stored with data, and bioreactor temperature, pressure, flow, the function such as setting and control of concentration and level parameter；Institute
Gsm module is stated, realizes the wireless monitor of industry spot, local PC can be used for remote control, and network PC is extended to rising
Network control；The embedded microprocessor, it is desirable to provide stronger control ability, USART interfaces, ADC conversion functions,
LCD touch screen and USB port etc., substantial amounts of data operation and processing will be carried out to the bioreactor population parameter.
A kind of bioreactor population parameter detecting system and its measurement control method, the bioreactor measure controlling party
Method, automatic control flow chart as shown in figure 3, the control of the temperature of bioreactor, pressure, flow, concentration and liquid level using each
The fuzzy control of autoregressive parameter, setting value and measured value are sent into fuzzy control network after fuzzy division, and generation controls subset,
The frequency values and duty cycle of PWM pulsewidth modulations are produced by converter, then generate PWM drive signal, driving power electronics
Device, power supply are exported to motor and automatic valve, by reactor sensor and adjustment circuit, form corresponding parametric values and parameter
Change rate, is adjusted output frequency and duty cycle according to corresponding parametric values and parameter change rate controller, by biology
Startup, stopping and the frequency of reactor motor and corresponding automatic valve switch-mode regulation are set and controlled, and realize bioreactor
Relevant parameter Detection ＆ Controling；Material pH value in reactor is measured by pH value measuring instrument, is measured by dissolved oxygen sensing instrument anti-
Dissolved oxygen concentration in device is answered, passes through O2Oxygen concentration in concentration meter measurement reactor, passes through CO2CO in concentration meter measurement reactor2's
Concentration, weight of material in reactor is measured by weighing instrument, is passed to control system by sensor on-line checking and pick-up and is realized
Bioreactor population parameter Detection ＆ Controling.
The beneficial effects of the invention are as follows：
The present invention provides a kind of bioreactor population parameter detecting system and its measurement control method, can preferably solve
The detection of all kinds of parameter online datas of bioreactor and collection, realize the temperature of bioreactor, pressure, flow, concentration with
The characteristics of liquid level automatically controls, binding reactor, fusion various processes theory and control theory, that detects and collect is each
Class supplemental characteristic carries out calculating processing, analysis obtain the oxygen uptake rates of bioprocesses, carbon dioxide production rate, breathing entropy,
Volumetric Oxygen Transfer Coefficients, than growth rate etc., realize the optimization of bioprocesses.
Brief description of the drawings
Fig. 1 is the bioreactor population parameter detecting system composition schematic diagram of the present invention；
In Fig. 1：1. bioreactor, 2. blenders, 3. electronic weighing instruments, 4. baiting valves, 5. fluid level transmitters, 6. pH are surveyed
Measure instrument, 7. dissolved oxygen sensing instrument, 8. temperature transmitters, 9. control systems, 10. O2Concentration meter, 11. CO2Concentration meter, 12. stirrings
Motor and frequency converter, 13. pressure sensors, 14. air valves, 15. air flow meters, 16. cooling water valves, 17. cooling waters
Flowmeter, 18. inlet valves；
Fig. 2 is the bioreactor, control system composition schematic diagram of the present invention；
Fig. 3 is the bioreactor automatic control flow chart schematic diagram of the present invention.
Embodiment
The invention will be further described with embodiment below in conjunction with the accompanying drawings：
A kind of bioreactor population parameter detecting system, as shown in Figure 1, by bioreactor 1, blender 2, electronic weighing instrument 3,
Outlet valve 4, fluid level transmitter 5, pH measuring instruments 6, dissolved oxygen sensing instrument 7, temperature transmitter 8, control system 9, O2Concentration meter 10,
CO2Concentration meter 11, stirring motor and frequency converter 12, pressure sensor 13, air valve 14, air flow meter 15, cooling water valve 16,
Cooling water flow meter 17, inlet valve 18, composition.
Control system 9 described in a kind of bioreactor population parameter detecting system, as shown in Fig. 2, mainly being declined by insertion
It is controller and field programmable gate array (FPGA) device, measurement and control of temperature module, pressure testing and controlling module, flow measuring and controlling module, dense
Spend control module, pH value control module, weight control module, gsm module, local and network PC, LCD control, various sensors,
Printer and detection control module composition；Mainly by embedded microcontroller and field programmable gate array (FPGA) device, temperature
Degree adjustment module, pressure adjusting module, gsm module, local and network PC, LCD control, various sensors, printer and detection,
Control module forms, as shown in Fig. 2, realizing the functions such as the parameter detecting of bioreactor, live display, field control, at the same time
Realize the automated control of bioreactor temperature, pressure, flow, concentration and liquid level；Programmable gate array (FPGA) device
Part, there is provided a large amount of ram spaces preserve intermediate parameters and computation of table lookup, there is provided substantial amounts of general purpose I/O Interface, for input and output
Module and the accurate PWM modulation function of quick response；The detection measurement module, is mainly adjusted and is adopted by sensor, signal
The composition such as collection, realizes the measurement of bioreactor temperature, pressure, flow, concentration and liquid level and the state of each critical piece；Institute
State control module to be mainly made of driving and executing agency, realize the driving and control of air valve, water valve, motor, frequency converter etc.；
The LCD touch screen, IDE hard disks, printer co-ordination are to realize the real-time collection of bioreactor population parameter, display, defeated
Go out and stored with data, and bioreactor temperature, pressure, flow, the function such as setting and control of concentration and level parameter；Institute
Gsm module is stated, realizes the wireless monitor of industry spot, local PC can be used for remote control, and network PC is extended to rising
Network control；The embedded microprocessor, it is desirable to provide stronger control ability, USART interfaces, ADC conversion functions,
LCD touch screen and USB port etc., will carry out the bioreactor population parameter substantial amounts of data operation, processing and system control.
A kind of bioreactor population parameter detecting system and its measurement control method, the temperature survey of the bioreactor
Fuzzy control is used with control, automatic control flow chart is surveyed through temperature sensor 8 as shown in figure 3, set specific temperature of reactor
Fixed temperature of reactor is sent into fuzzy control network, generation control subset, PWM is produced by converter after fuzzy division
The frequency values and duty cycle of pulsewidth modulation, then generate PWM drive signal, driving power electronic device, and power supply is exported to cold
But water valve 16, by cooling water flow meter 17 and adjustment circuit, form respective streams value and flow change rate, according to flow value and
Flow change rate controller is adjusted output frequency and duty cycle, by the way that the switch-mode regulation of cooling water valve 16 is set and controlled
The flow of refrigeration but water ga(u)ge 17, realizes the Detection ＆ Controling of bioreactor temperature.
A kind of bioreactor population parameter detecting system and its measurement control method, the pressure measurement of the bioreactor
Fuzzy control is used with control, automatic control flow chart is as shown in figure 3, set specific reactor pressure, through pressure sensor 13
The reactor pressure of measure is sent into fuzzy control network, generation control subset, is produced by converter after fuzzy division
The frequency values and duty cycle of PWM pulsewidth modulations, then generate PWM drive signal, driving power electronic device, adjusts air valve
14, by adjusting the flow and adjustment circuit of air flow meter 15, form 15 flow value of respective air flowmeter and changes in flow rate
Rate, is adjusted output frequency and duty cycle according to flow value and flow change rate controller, passes through opening to air valve 14
Close adjusting to be set and controlled, 15 flow of air flow meter realizes the Detection ＆ Controling of 1 pressure of bioreactor.
A kind of bioreactor population parameter detecting system and its measurement control method, the flow measurement of the bioreactor
And control, the flow of cooling water flow meter 17 adjust the aperture of cooling water valve 16 to realize by control system 9；Air flow meter 15
Flow the aperture of air valve 14 is adjusted to realize by control system 9, realize the Detection ＆ Controling of bioreactor flow；
A kind of bioreactor population parameter detecting system and its measurement control method, the level gauging of the bioreactor and control
System, the night position in bioreactor 1 is measured by fluid level transmitter 4, opening for inlet valve 18 and outlet valve 4 is adjusted by control system 9
Spend to realize the Detection ＆ Controling of bioreactor liquid level.
A kind of bioreactor population parameter detecting system and its measurement control method, the pH value measurement of the bioreactor
And control, material pH value in reactor is measured by pH value measuring instrument 6；The oxygen concentration testing of the bioreactor and control,
Material dissolution oxygen concentration in reactor is measured by dissolved oxygen sensing instrument 7, passes through O2Concentration meter 10 measures gas phase oxygen in reactor
Concentration；The CO of the bioreactor2Measurement of concetration and control, pass through CO2Concentration meter 11 measures CO in reactor2Concentration；It is described
The weight measurement and control of bioreactor, weight of material in reactor is measured by electronic weighing instrument 3；Above-mentioned measurement data is simultaneously
Control system 9 is passed to by sensor pick-up, control system 9 carries out data calculating and processing, will according to reaction process and control
Progress is asked to adjust and control accordingly, to realize bioreactor population parameter Detection ＆ Controling.
The present invention is not limited to the above embodiments, and without departing from the spirit and scope of the present invention, the present invention is also
Various changes and modifications are had, these changes and improvements are both fallen within protection scope of the present invention.
Claims (7)
1. a kind of bioreactor population parameter detecting system and its measurement control method, it is characterized in that, the bioreactor is complete
Parameter detecting system, as shown in Figure 1, being measured by bioreactor, blender electricity, electronic weighing instrument, fluid level transmitter, pH value
Instrument, dissolved oxygen sensing instrument, temperature transmitter, control system, O2Concentration meter, CO2Concentration meter, pressure sensor, air valve, air
Flowmeter, cooling water valve, cooling water flow meter, weighing instrument, inlet valve and baiting valve form.
2. a kind of bioreactor population parameter detecting system as claimed in claim 1 and its measurement control method, it is characterized in that,
The control system, as shown in Fig. 2, mainly by embedded microcontroller and field programmable gate array (FPGA) device, temperature
Control module, pressure testing and controlling module, flow measuring and controlling module, concentration control module, pH value control module, weight control module, GSM
Module, local and network PC, LCD control, various sensors, printer and detection control module composition；Realize bioreactor
Parameter detecting, live display, the function such as field control, while realize bioreactor temperature, pressure, flow, concentration and liquid
The automated control of position；Programmable gate array (FPGA) device, there is provided a large amount of ram spaces preserve intermediate parameters and table look-up
Calculate, there is provided substantial amounts of general purpose I/O Interface, for input/output module and the accurate PWM modulation function of quick response；It is described
Detect measurement module, mainly by sensor, signal adjust and collection etc. form, realize bioreactor temperature, pressure, flow,
The measurement of concentration and liquid level and the state of each critical piece；The control module is mainly made of driving and executing agency, real
The driving and control of existing air valve, water valve, motor, frequency converter etc.；The LCD touch screen, IDE hard disks, printer co-ordination
To realize that the real-time collection of bioreactor population parameter, display, output are stored with data, and bioreactor temperature, pressure,
The functions such as the setting and control of flow, concentration and level parameter；The gsm module, realizes the wireless monitor of industry spot, local
PC can be used for remote control, and network PC is extended to the network control risen；The embedded microprocessor, it is desirable to provide
Stronger control ability, USART interfaces, ADC conversion functions, LCD touch screen and USB port etc., to the bioreactor population parameter
Carry out substantial amounts of data operation and processing.
3. a kind of bioreactor population parameter detecting system and its measurement controlling party as described in claim 1 and claim 2
Method, it is characterized in that, the temperature measure and control of the bioreactor uses fuzzy control, automatic control flow chart as shown in figure 3,
Specific temperature of reactor is set, through the temperature of reactor of temperature sensor measurement after fuzzy division, is sent into fuzzy control
Network, generation control subset, the frequency values and duty cycle of PWM pulsewidth modulations are produced by converter, then generate PWM drivings
Signal, driving power electronic device, power supply are exported to cooling water valve, by cooling water flow meter and adjustment circuit, are formed corresponding
Flow value and flow change rate, are adjusted output frequency and duty cycle according to flow value and flow change rate controller, lead to
Cross the flow for the switch-mode regulation of cooling water valve being set and controlled cooling water flow meter, realize the detection of bioreactor temperature with
Control.
4. a kind of bioreactor population parameter detecting system and its measurement controlling party as described in claim 1 and claim 2
Method, it is characterized in that, the pressure measurement of the bioreactor and control use fuzzy control, automatic control flow chart as shown in figure 3,
Specific reactor pressure is set, through the reactor pressure of determination of pressure sensor after fuzzy division, is sent into fuzzy control
Network, generation control subset, the frequency values and duty cycle of PWM pulsewidth modulations are produced by converter, then generate PWM drivings
Signal, driving power electronic device, adjusts air valve, by adjusting the flow and adjustment circuit of air flow meter, is formed corresponding
Air flow meter flow value and flow change rate, according to flow value and flow change rate controller to output frequency and duty cycle into
Row adjustment, by the way that the switch-mode regulation of air valve is set and controlled, air flow meter flow realizes the inspection of bioreactor pressure
Survey and control.
5. a kind of bioreactor population parameter detecting system and its measurement controlling party as described in claim 1 and claim 2
Method, it is characterized in that, the flow measurement and control of the bioreactor, the flow of cooling water flow meter are adjusted cold by control system
But the aperture of water valve is realized；The flow of air flow meter is realized by the aperture of control system adjusting air valve, realizes biology
The Detection ＆ Controling of reactor flow.
6. a kind of bioreactor population parameter detecting system and its measurement controlling party as described in claim 1 and claim 2
Method, it is characterized in that, the level gauging of the bioreactor and control, by the night in fluid level transmitter measurement bioreactor
Position, the Detection ＆ Controling of bioreactor liquid level are realized by the aperture of control system adjusting inlet valve and outlet valve.
7. a kind of bioreactor population parameter detecting system and its measurement controlling party as described in claim 1 and claim 2
Method, it is characterized in that, the pH value measurement and control of the bioreactor, material pH in reactor is measured by pH value measuring instrument
Value；The oxygen concentration testing of the bioreactor and control, it is dense to measure material dissolution oxygen in reactor by dissolved oxygen sensing instrument
Degree, passes through O2Oxygen concentration of gas phase in concentration meter measurement reactor；The CO of the bioreactor2Measurement of concetration and control, pass through
CO2CO in concentration meter measurement reactor2Concentration；The weight measurement and control of the bioreactor, are measured by electronic weighing instrument
Weight of material in reactor；Above-mentioned measurement data simultaneously passes to control system by sensor pick-up, and control system carries out data meter
Calculate and handle, require to carry out adjusting accordingly according to reaction process and control and control, to realize that bioreactor population parameter is examined
Survey and control.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108854883A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction system and its working method with monitor terminal |
| CN108854882A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction operating system and its working method that convenience remotely controls |
| CN109456877A (en) * | 2018-11-15 | 2019-03-12 | 上海量能生物科技有限公司 | The bioreactor of touch control operation |
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| CN108854882A (en) * | 2018-07-09 | 2018-11-23 | 马伟超 | A kind of chemical reaction operating system and its working method that convenience remotely controls |
| CN108854883B (en) * | 2018-07-09 | 2020-12-18 | 山东信发化工有限公司 | Chemical reaction system with monitoring terminal and working method thereof |
| CN108854882B (en) * | 2018-07-09 | 2020-12-18 | 山东信发化工有限公司 | Chemical reaction operation system convenient for remote control and working method thereof |
| CN109456877A (en) * | 2018-11-15 | 2019-03-12 | 上海量能生物科技有限公司 | The bioreactor of touch control operation |
| CN109609378A (en) * | 2018-12-20 | 2019-04-12 | 杭州洪扬生物工程有限公司 | A kind of bioreactor |
| CN109999527A (en) * | 2019-04-25 | 2019-07-12 | 青岛杰瑞工控技术有限公司 | A kind of multi-fluid intelligence ingredient control method |
| CN109999527B (en) * | 2019-04-25 | 2021-10-15 | 青岛杰瑞工控技术有限公司 | Multi-fluid intelligent batching control method |
| CN112007370A (en) * | 2020-09-23 | 2020-12-01 | 内蒙古通威高纯晶硅有限公司 | Material concentration control system and method |
| CN115287171A (en) * | 2022-07-07 | 2022-11-04 | 百仑生物科技(江苏)有限公司 | Multi-parameter detection control system for bioreactor |
| CN115287171B (en) * | 2022-07-07 | 2023-09-22 | 百仑生物科技(江苏)有限公司 | Multi-parameter detection control system of bioreactor |
| CN118491443A (en) * | 2024-07-19 | 2024-08-16 | 南通永盛化工有限公司 | Quantitative control system for iminodiacetic acid production |
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