CN109554294A - A kind of continuous record growth curve of bacteria device - Google Patents
A kind of continuous record growth curve of bacteria device Download PDFInfo
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- CN109554294A CN109554294A CN201811606997.XA CN201811606997A CN109554294A CN 109554294 A CN109554294 A CN 109554294A CN 201811606997 A CN201811606997 A CN 201811606997A CN 109554294 A CN109554294 A CN 109554294A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 81
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 238000003756 stirring Methods 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 230000008676 import Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- BGOFCVIGEYGEOF-UJPOAAIJSA-N helicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1C=O BGOFCVIGEYGEOF-UJPOAAIJSA-N 0.000 claims description 3
- 238000005259 measurement Methods 0.000 abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 abstract description 6
- 239000001301 oxygen Substances 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 4
- 238000005086 pumping Methods 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 description 8
- 230000006872 improvement Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The present invention discloses a kind of continuous record growth curve of bacteria device, bacterium solution is put into culture vessel and is cultivated, heating tube is controlled by temperature detect switch (TDS) to the heating temperature of bacterium solution, bacterium solution temperature real-time display is on temperature display, the survival rate of bacterium can be improved, rabbling mechanism drives transmission shaft that agitating paddle is driven to rotate by stirring motor, agitating paddle is to bacterium solution automatic stirring, improve the dissolved oxygen amount of bacterium solution, the pH value that PH measuring mechanism passes through the electronics PH meter measurement bacterium solution of measuring rod lower end, PH electronic recorder records the real-time pH value of bacterium solution, light splitting degree measuring mechanism is drawn into bacterium solution in point photometric instrument by pressure pump, and point photometric instrument can measure the light splitting degree in the bacterium solution for recording and pumping and, bacterium solution after measurement passes through return pipe again and sends back in culture vessel, and then it can continuously detect the P of bacterium solution H value, light splitting degree, can continuously record growth curve of bacteria, reduce the workload of people.The present invention is used for the measurement to growth curve of bacteria and records.
Description
Technical field
The present invention relates to the field of microorganism detection, in particular to a kind of continuous record growth curve of bacteria device.
Background technique
It with bacterial number variation is vertical by abscissa of the time of culture when bacterium is cultivated under suitable environmental condition
Coordinate, making a reacting bacteria according to the relationship between bacterial number variation and corresponding time change, bacterium number becomes in the training period
This curve of the curve of law is known as growth curve.Bacterial number is all by light splitting degree measuring instrument, with 500~600nm
Light wave measures the transmitted light beam light intensity of the light beam through the bacterial solution in cuvette.Langbobier law (Beer-
Lambert Law) think that the ratio that light is absorbed by transparent medium is unrelated with the intensity of incident light, every blanket layer medium on light path
Absorb the light of same ratio value.Pass through langbobier law.The OD value of bacterial solution can be calculated, the concentration of bacterial solution and thin
Bacterium measurement OD value is positively correlated in a certain range, such as in the range of OD value is 0.05~0.5, can be based on experience value
The concentration for the bacterial solution that converts, the method that traditional Bacteria cold shock measurement uses manual measurement, in conical flask culture bacterium,
It is sampled from bacteria culture bottle according to the time point of design every more than ten minutes, is put into cuvette, is measured with spectrophotometric
The OD value for measuring bacterium, by measuring a series of OD value at time points, to observe Bacteria cold shock, time-consuming, operates numerous
It is trivial；And the pH value in detection bacterium bacterium solution is also wanted, because acid or alkalinity excrement can be discharged in bacterium during growth
Just, method existing and by manual measurement, at regular intervals detects bacterial solution with PH meter, greatly improves people
Workload, and cannot accomplish to detect in real time, there are also in order to improve oxygen amount in bacterium solution, artificial timing be needed to carry out bacterium solution
Stirring, so that bacterium solution absorbs the oxygen in atmosphere, by the control of temperature, temperature is excessively high or too low all can for the growing state of bacterium
Influence the survival rate of bacterium.
Summary of the invention
The technical problem to be solved by the present invention is providing a kind of continuous record growth curve of bacteria device, can solve to survey thin
Bacterium growth curve labor intensive, problem complicated for operation improve accuracy, the continuity of measurement.
The solution that the present invention solves its technical problem is:
A kind of continuous record growth curve of bacteria device, including being in vertically arranged culture vessel, the culture vessel
Upper end be equipped with opening, the opening is stamped in the lid that is laterally arranged, the lid and the releasable connection that is open, in lid
It is equipped with first through hole, the second through-hole, third through-hole, fifth hole, the first through hole, the second through-hole, third through-hole, the
Five through-holes, which are distributed in distance, to be arranged on lid, and the lateral wall of the culture vessel is surrounded with spiral helicine heating tube, is training
The inside for supporting container is equipped with temperature sensor, is equipped with temperature detect switch (TDS), temperature display in the lateral wall of culture vessel, described
Temperature detect switch (TDS) and heating tube be electrically connected, the temperature display and temperature sensor are electrically connected, and are installed on lid
There are rabbling mechanism, PH measuring mechanism, light splitting degree measuring mechanism, the rabbling mechanism includes the stirring being arranged in culture vessel
Paddle, the agitating paddle are connected in vertically arranged transmission shaft, and the lower end of the transmission shaft is connect with agitating paddle, in lid
Upper wall surface stirring motor is installed, the upper end of the transmission shaft is driven after passing through first through hole from the bottom up with stirring motor
Connection, the PH measuring mechanism include in vertically arranged measuring rod, and the lower end of the measuring rod passes through the from top to bottom
Two through-holes are extended back into culture vessel, and the lower end of the measuring rod is equipped with electronics PH meter, and the measuring rod is set in hollow
It sets, conducting wire is installed in measuring rod, one end of the conducting wire and electronics PH meter are electrically connected, the other end electricity of the conducting wire
Property be connected with PH electronic recorder, the light splitting degree measuring mechanism includes dividing photometric instrument, and described divides photometric instrument
The concentration of detectable record bacterium solution, described that photometric instrument is divided to be equipped with inlet, liquid outlet, the described photometric instrument of dividing
Inlet is connected with pressure pump, and the pressure pump is equipped with import, outlet, and the outlet of the pressure pump passes through conduit and light splitting
The inlet connection of measuring instrument is spent, the import of the pressure pump is connected with inlet tube, is equipped in the side wall of bottom of culture vessel
Fourth hole, the pressure pump are connect by inlet tube with fourth hole, and the third through-hole is set in vertically arranged
Return pipe, the lower end of the return pipe passes through third through-hole from top to bottom and extendes back into culture vessel, the return pipe
Upper end be connected to the liquid outlet of point photometric instrument by conduit.
As a further improvement of the foregoing solution, it is covered with insulating layer in the lateral wall of culture vessel, the heating tube is set
It sets between insulating layer and the lateral wall of culture vessel.
As a further improvement of the foregoing solution, the fifth hole is equipped with multiple, and multiple fifth holes are uniformly distributed and cover
On body.
As a further improvement of the foregoing solution, the lateral wall of the measuring rod is equipped with external screw thread, and described second is logical
The inner sidewall in hole, which is equipped with, to be connect with the matched internal screw thread of external screw thread, the measuring rod with the second through hole whorl.
As a further improvement of the foregoing solution, the lateral wall of the return pipe is set with fixed ring, the reflux
Pipe is fixedly connected by fixed ring with the upper side wall of lid, and the upper end of the return pipe is connected with flange, the return pipe
It is connect by flange with conduit.
As a further improvement of the foregoing solution, it is equipped in the upper wall surface of lid in vertically arranged fixed plate, it is described
The bottom of fixed plate be fixedly connected with the upper wall surface of lid, it is in vertically arranged sliding rail that the side wall of the fixed plate, which is equipped with,
The stirring motor is connected with slide plate, and the stirring motor is fixedly mounted on slide plate, and the slide plate and sliding rail slide
Connection, so that the stirring motor can be moved up and down along sliding rail, the slide plate is equipped with lock-screw, the locking screw
Nail is threadedly coupled with slide plate, and the end of the lock-screw abuts after passing through slide plate inside outside with fixed plate, thus fixed
The position of slide plate on the slide rail.
As a further improvement of the foregoing solution, the top and bottom of the sliding rail are mounted on limited block, limit institute
The slide plate stated slides up and down between two limited blocks.
The beneficial effects of the present invention are: bacterium solution is put into culture vessel first in the growth curve of measurement record bacterium
It is inside cultivated, and heating tube can be controlled by temperature detect switch (TDS) to the heating temperature of bacterium solution, bacterium solution temperature real-time display is in temperature
It spends on display screen, and then the survival rate of bacterium can be improved, rabbling mechanism drives transmission shaft to drive agitating paddle by stirring motor
Rotation, agitating paddle is to bacterium solution automatic stirring, to improve the dissolved oxygen amount of bacterium solution, PH measuring mechanism passes through the electronics of measuring rod lower end
The pH value of PH meter measurement bacterium solution, PH electronic recorder records the real-time pH value of bacterium solution, and degree of light splitting measuring mechanism passes through
Pressure pump is drawn into bacterium solution in point photometric instrument, and point photometric instrument can measure the light splitting in the bacterium solution for recording and pumping and
Degree, the bacterium solution after measurement passes through return pipe again and sends back in culture vessel, and then can continuously detect the pH value of bacterium solution, light splitting degree,
Growth curve of bacteria can be continuously recorded, the workload of people is reduced, improves accuracy, the continuity of measurement.
The present invention is used for the measurement to growth curve of bacteria and records.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is a part of the embodiments of the present invention, rather than is all implemented
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is the whole partial sectional view of the embodiment of the present invention.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to design of the invention, specific structure and generation clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of the embodiment, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art are not being paid
Other embodiments obtained, belong to the scope of protection of the invention under the premise of creative work.In addition, be previously mentioned in text
All connection/connection relationships not singly refer to that component directly connects, and referring to can be added deduct according to specific implementation situation by adding
Few couple auxiliary, Lai Zucheng more preferably coupling structure.Each technical characteristic in the present invention, in the premise of not conflicting conflict
Under can be with combination of interactions.
Referring to Fig.1, this is the embodiment of the present invention, specifically:
A kind of continuous record growth curve of bacteria device, including being in vertically arranged culture vessel 100, the culture
The upper end of container 100 is equipped with opening 110, and the opening 110 is stamped in the lid 200 that is laterally arranged, the lid 200 with
Be open 110 releasable connections, and first through hole 210, the second through-hole 220, third through-hole 230, fifth hole are equipped on lid 200
280, the first through hole 210, the second through-hole 220, third through-hole 230, fifth hole 280, which are distributed in distance, to be arranged in lid
On 200, the lateral wall of the culture vessel 100 is surrounded with spiral helicine heating tube 300, pacifies in the inside of culture vessel 100
Equipped with temperature sensor 310, temperature detect switch (TDS) 320, temperature display 330 are installed in the lateral wall of culture vessel 100, it is described
Temperature detect switch (TDS) 320 and heating tube 300 are electrically connected, and the temperature display 330 and temperature sensor 310 are electrically connected,
Rabbling mechanism, PH measuring mechanism, light splitting degree measuring mechanism are installed, the rabbling mechanism includes that setting is being trained on lid 200
The agitating paddle 400 in container 100 is supported, the agitating paddle 400 is connected in vertically arranged transmission shaft 410, the transmission
The lower end of axis 410 is connect with agitating paddle 400, is equipped with stirring motor 420, the transmission shaft 410 in the upper wall surface of lid 200
Upper end pass through be sequentially connected after first through hole 210 with stirring motor 420 from the bottom up, the PH measuring mechanism includes in perpendicular
To the measuring rod 500 of setting, the lower end of the measuring rod 500 passes through the second through-hole 220 from top to bottom and extendes back into culture vessel
In 100, the lower end of the measuring rod 500 is equipped with electronics PH meter 510, and the measuring rod 500 is in hollow setting, is measuring
Be installed with conducting wire 520 in bar 500, one end of the conducting wire 520 and electronics PH meter 510 are electrically connected, the conducting wire 520 it is another
One end is electrically connected with PH electronic recorder 530, and the light splitting degree measuring mechanism includes dividing photometric instrument 600, described
Divide the concentration of the detectable record bacterium solution of photometric instrument 600, described divides photometric instrument 600 to be equipped with inlet, liquid outlet, institute
The inlet for point photometric instrument 600 stated is connected with pressure pump 610, and the pressure pump 610 is equipped with import, outlet, described
The outlet of pressure pump 610 be connected to the inlet of point photometric instrument 600 by conduit, the import of the pressure pump 610
Be connected with inlet tube 620,100 bottom of culture vessel side wall be equipped with fourth hole 120, the pressure pump 610 by into
Liquid pipe 620 is connect with fourth hole 120, and the third through-hole 230 is set in vertically arranged return pipe 630, described
The lower end of return pipe 630 passes through third through-hole 230 from top to bottom and extendes back into culture vessel 100, the return pipe 630
Upper end is connected to by conduit with the liquid outlet of point photometric instrument 600.In the growth curve of measurement record bacterium, first bacterium
Liquid is put into culture vessel 100 and is cultivated, and heating tube 300 can be controlled by temperature detect switch (TDS) 320 to the heating temperature of bacterium solution
The survival rate of bacterium can be improved on temperature display 330 in degree, bacterium solution temperature real-time display, and rabbling mechanism passes through stirring
Motor 420 drives transmission shaft 410 to drive agitating paddle 400 to rotate, and agitating paddle 400 is to bacterium solution automatic stirring, to improve bacterium solution
Dissolved oxygen amount, pH values of the PH measuring mechanism by the electronics PH of 500 lower end of measuring rod meter, 510 measurement bacterium solutions, PH electronic recorder
530 real-time pH values of bacterium solution are recorded, and bacterium solution is drawn into light splitting degree by pressure pump 610 and surveyed by degree of light splitting measuring mechanism
It measures on instrument 600, and point photometric instrument 600 can measure the light splitting degree in the bacterium solution for recording and pumping and, the bacterium solution after measurement is led to again
It crosses return pipe 630 to send back in culture vessel 100, and then can continuously detect the pH value of bacterium solution, light splitting degree, can continuously record bacterium
Growth curve reduces the workload of people, improves accuracy, the continuity of measurement, the present apparatus is by the power supply of peripheral hardware to electricity consumption member
Part is powered, and the fifth hole 280 on lid 200 is connected to culture vessel 100 with outside, and atmosphere can pass through fifth hole
280 enter in culture vessel 100, and the mode that lid 200 can be connected through a screw thread with opening 110 is fixed.
It is further used as preferred embodiment, is covered with insulating layer 130 in the lateral wall of culture vessel 100, described adds
Heat pipe 300 is arranged between insulating layer 130 and the lateral wall of culture vessel 100.Insulating layer 130 mainly reduces heat waste, so that plus
Most of heat of heat pipe 300 is conducted toward the inside of culture vessel 100, to reduce energy consumption, plays energy-saving and environment-friendly effect.
It is further used as preferred embodiment, the fifth hole 280 is equipped with multiple, and multiple fifth holes 280 are
On cloth and lid 200.Multiple fifth holes 280, which are arranged, makes circulation of the atmosphere in culture vessel 100 more smooth, and raising is stirred
The effect for mixing Agitated vessel improves the dissolved oxygen amount of bacterium solution.
It is further used as preferred embodiment, the lateral wall of the measuring rod 500 is equipped with external screw thread, and described second
The inner sidewall of through-hole 220, which is equipped with, to be threadedly coupled with the matched internal screw thread of external screw thread, the measuring rod 500 with the second through-hole 220.
According to the bacterium solution of not co-content, can turn measuring rod 500 adjust height of the electronics PH meter 510 in culture vessel 100, and
The phenomenon that measuring rod 500 is threadedly coupled the stability that measuring rod 500 can be improved with the second through-hole 220, prevents inclination, and shadow
Ring the normal work for arriving agitating paddle 400.
It is further used as preferred embodiment, the lateral wall of the return pipe 630 is set with fixed ring 631, described
Return pipe 630 be fixedly connected by fixed ring 631 with the upper side wall of lid 200, the upper end of the return pipe 630 is connected with
Flange 632, the return pipe 630 are connect by flange 632 with conduit.Fixed ring 631 improves the fastness of return pipe 630,
It avoids return pipe 630 from tilting, improves the fluency of bacterium solution reflux.
It is further used as preferred embodiment, is equipped in the upper wall surface of lid 200 in vertically arranged fixed plate
240, the bottom of the fixed plate 240 is fixedly connected with the upper wall surface of lid 200, and the side wall of the fixed plate 240 is equipped with
In vertically arranged sliding rail 250, the stirring motor 420 is connected with slide plate 260, and the stirring motor 420 is fixedly mounted
On slide plate 260, the slide plate 260 is slidably connected with sliding rail 250, so that the stirring motor 420 can be along sliding rail 250
Lower movement, the slide plate 260 are equipped with lock-screw 270, and the lock-screw 270 is threadedly coupled with slide plate 260, described
The end of lock-screw 270 abut after passing through slide plate 260 inside outside with fixed plate 240, so that fixed glides 260 are in sliding rail
Position on 250.When being stirred to different amounts of bacterium solution, by upper and lower position of the adjusting slider 260 on sliding rail 250, and
And can also be by lock-screw 270 come the position of fixed glides 260, and then adjust the height of agitating paddle 400.
It is further used as preferred embodiment, the top and bottom of the sliding rail 250 are mounted on limited block 251,
The slide plate 260 is limited to slide up and down between two limited blocks 251.This can anti-limited slip plate 260 move out stroke, it is right
Agitating paddle 400 plays the role of protection, and agitating paddle 400 is effectively prevent to touch the bottom of lid 200 or culture vessel 100.
Better embodiment of the invention is illustrated above, but the present invention is not limited to the embodiment,
Those skilled in the art can also make various equivalent modifications or replacement on the premise of without prejudice to spirit of the invention, this
Equivalent variation or replacement are all included in the scope defined by the claims of the present application a bit.
Claims (7)
1. a kind of continuous record growth curve of bacteria device, it is characterised in that: including being in vertically arranged culture vessel (100),
The upper end of the culture vessel (100) is equipped with opening (110), and the opening (110) is stamped in the lid being laterally arranged
(200), the lid (200) and opening (110) releasable connection, are equipped with first through hole (210), second on lid (200)
Through-hole (220), third through-hole (230), fifth hole (280), the first through hole (210), the second through-hole (220), third
Through-hole (230), fifth hole (280), which are distributed in distance, to be arranged on lid (200), the outside of the culture vessel (100)
Wall is surrounded with spiral helicine heating tube (300), is equipped with temperature sensor (310) in the inside of culture vessel (100), is cultivating
The lateral wall of container (100) is equipped with temperature detect switch (TDS) (320), temperature display (330), the temperature detect switch (TDS) (320) with plus
Heat pipe (300) is electrically connected, and the temperature display (330) and temperature sensor (310) are electrically connected, in lid (200)
On rabbling mechanism, PH measuring mechanism, light splitting degree measuring mechanism be installed, the rabbling mechanism includes being arranged in culture vessel
(100) agitating paddle (400) in, the agitating paddle (400) are connected in vertically arranged transmission shaft (410), the biography
The lower end of moving axis (410) is connect with agitating paddle (400), is equipped with stirring motor (420) in the upper wall surface of lid (200), described
Transmission shaft (410) upper end pass through from the bottom up first through hole (210) afterwards with stirring motor (420) be sequentially connected, it is described
PH measuring mechanism includes in vertically arranged measuring rod (500), and the lower end of the measuring rod (500) passes through second from top to bottom
Through-hole (220) is extended back into culture vessel (100), and the lower end of the measuring rod (500) is equipped with electronics PH meter (510), institute
The measuring rod (500) stated is in hollow setting, is installed with conducting wire (520) in measuring rod (500), one end of the conducting wire (520)
It is electrically connected with electronics PH meter (510), the other end of the conducting wire (520) is electrically connected with PH electronic recorder (530), institute
The light splitting degree measuring mechanism stated includes point photometric instrument (600), and described divides photometric instrument (600) detectable record bacterium solution
Concentration, it is described that photometric instrument (600) is divided to be equipped with inlet, liquid outlet, the feed liquor for dividing photometric instrument (600)
Mouth is connected with pressure pump (610), and the pressure pump (610) is equipped with import, outlet, and the outlet of the pressure pump (610) is logical
It crosses conduit to be connected to the inlet of a point photometric instrument (600), the import of the pressure pump (610) is connected with inlet tube
(620), the side wall in culture vessel (100) bottom is equipped with fourth hole (120), and the pressure pump (610) passes through inlet tube
(620) it is connect with fourth hole (120), the third through-hole (230) is set in vertically arranged return pipe (630), institute
The lower end for the return pipe (630) stated passes through third through-hole (230) from top to bottom and extendes back into culture vessel (100), and described returns
The upper end of flow tube (630) is connected to by conduit with the liquid outlet of a point photometric instrument (600).
2. a kind of continuous record growth curve of bacteria device according to claim 1, it is characterised in that: in culture vessel
(100) lateral wall is covered with insulating layer (130), and the heating tube (300) is arranged in insulating layer (130) and culture vessel
(100) between lateral wall.
3. a kind of continuous record growth curve of bacteria device according to claim 1, it is characterised in that: described the 5th is logical
Hole (280) be equipped with it is multiple, multiple fifth holes (280) are uniformly distributed and lid (200) on.
4. a kind of continuous record growth curve of bacteria device according to claim 1, it is characterised in that: the measuring rod
(500) lateral wall is equipped with external screw thread, the inner sidewall of second through-hole (220) be equipped with the matched internal screw thread of external screw thread,
The measuring rod (500) is threadedly coupled with the second through-hole (220).
5. a kind of continuous record growth curve of bacteria device according to claim 1, it is characterised in that: the return pipe
(630) lateral wall is set with fixed ring (631), and the return pipe (630) passes through fixed ring (631) and lid (200)
Upper side wall is fixedly connected, and the upper end of the return pipe (630) is connected with flange (632), and the return pipe (630) passes through method
Blue (632) are connect with conduit.
6. a kind of continuous record growth curve of bacteria device according to claim 1, it is characterised in that: in lid (200)
Upper wall surface be equipped in vertically arranged fixed plate (240), the bottom of the fixed plate (240) is upper with lid (200)
Wall surface is fixedly connected, and it is in vertically arranged sliding rail (250), the stirring motor that the side wall of the fixed plate (240), which is equipped with,
(420) it is connected with slide plate (260), the stirring motor (420) is fixedly mounted on slide plate (260), the slide plate (260)
It is slidably connected with sliding rail (250), so that the stirring motor (420) can be moved up and down along sliding rail (250), the slide plate
(260) it is equipped with lock-screw (270), the lock-screw (270) is threadedly coupled with slide plate (260), the locking screw
The end of (270) is followed closely from abutting afterwards with fixed plate (240) across slide plate (260) inside outside, so that fixed glides (260) are in sliding rail
(250) position on.
7. a kind of continuous record growth curve of bacteria device according to claim 6, it is characterised in that: the sliding rail
(250) top and bottom are mounted on limited block (251), limit the slide plate (260) between two limited blocks (251)
It slides up and down.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811606997.XA CN109554294A (en) | 2018-12-27 | 2018-12-27 | A kind of continuous record growth curve of bacteria device |
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| CN201811606997.XA CN109554294A (en) | 2018-12-27 | 2018-12-27 | A kind of continuous record growth curve of bacteria device |
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Application publication date: 20190402 |