CN104988203A - Method for making quick microorganism detection test sheet - Google Patents
Method for making quick microorganism detection test sheet Download PDFInfo
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- CN104988203A CN104988203A CN201510294331.5A CN201510294331A CN104988203A CN 104988203 A CN104988203 A CN 104988203A CN 201510294331 A CN201510294331 A CN 201510294331A CN 104988203 A CN104988203 A CN 104988203A
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Abstract
The invention discloses a method for making a quick microorganism detection test sheet and relates to the technical field of quick microorganism detection. According to the technical scheme, mixed cold-water-soluble gel which is large in liquid suction amount, fast in liquid suction and capable of forming a network structure easily is adopted as bacterial colony gel, non-woven fabric which is fast in liquid suction and drainage is selected and used as a liquid suction material, and therefore the problems that the liquid suction speed is low and the lateral flow phenomenon is likely to happen are effectively solved; the gel mixing proportion of the gel which is large in liquid suction amount to the gel which is fast in liquid suction and capable of forming the network structure easily is 1:2 to 1:10. The gel is formed in a pre-gelling manner; 2 mL to 5 mL of 2* to 4* media are respectively and evenly dropped and the area of a medium region is at least 7 cm * 7 cm. Nutrients needed for microorganism growth are guaranteed, the media are evenly distributed, regular bacterial colonies are easily formed, and the bacterial colonies are prevented from dragging tails; writable waterproof colored adhesive tape is adopted as labels and used for distinguishing different detection sheets, an operator can write experiment records such as sample numbers, detection time and serial numbers on the colored adhesive tape, and operation is easy. In addition, the structure of a product is shown in the attached diagram.
Description
Technical field
The present invention relates to a kind of making of microbial rapid detection test-paper, art is microbiologic inhibition tests.
Background
At present, the inspection of China microorganism adopts traditional sensing techniques, i.e. flat band method, MPN method and biochemical identification method.Operation steps has configuration substratum, cleaning culture vessel, sterilizing, increasing bacterium, biochemical identification etc., and high to laboratory environment, equipment requirements, requisite space is large.Traditional sensing techniques is difficult to the demand meeting on-the-spot instant Testing and appraisal microorganism, be difficult to realize the real-time monitoring to microorganism, delayed the treatment time of polluting product, Pollution exposure crowd is difficult to be effectively controlled, so the microbial rapid detection test-paper technical products needed badly of market just.
At present, the patent that the product of this type of microorganism detection relates to has " USRFACE COLONY COUNTING DEVICE AND METHOD OF USE (US005681712A) " of Minnesota Mining and Manufacturing Company, " testing plate of a kind of rapid detection microorganism and its preparation method and application (ZL200910009717.3) " and " substratum fixing agent that cold water can coagulate and preparation method thereof (ZL200910039835.7) " of Guangdong Microbes Inst, the testing plate (ZL200520120355.0) of pathogenic micro-organism " in the food " of Lu Xin, " utilizing commercial product culture medium dry powder directly to make the method (ZL200710028489.3) of microorganism testing slice " of Guangzhou Oasis Biochemistry Technology Co., Ltd. and " the quick test scraps of paper (ZL03274715.2) of yeast and mold " of Oasis Biochemistry Research Center.
The Petrifilm microorganism test-paper series product rate of liquid aspiration of Minnesota Mining and Manufacturing Company is low, easily cause the effluent of sample, this series products domestic has the microbial rapid detection test-paper series product of Guangzhou Oasis Biochemistry Technology Co., Ltd., this series product consistence is poor, easy generation false negative result, accuracy is low.This invention technical products overcomes above product shortcoming.
Summary of the invention
The invention provides a kind of easy handling, Detection results be excellent, microbial rapid detection testing plate that accuracy rate is high, solve existing problem existing for microbial rapid detection technical products in the market.
Microbial rapid detection testing plate provided by the invention can detect the microorganisms such as total number of bacterial colony, coliform, intestinal bacteria, Salmonellas, Enterobacter sakazakii, streptococcus aureus, listeria spp, yeast and mold.
Present invention also offers the technical scheme of microbial rapid detection testing plate described above, specific as follows:
Coldwater-soluble gel that the present invention adopts liquid absorption large (as sodium carboxymethylcellulose pyce, ethyl cellulose sodium and sodium polyacrylate) and the fast and mixture easily forming the coldwater-soluble gel (as Viscogum BE, xanthan gum and guar gum etc.) of gel network structure of liquid absorption as the gel support formed needed for bacterium colony, select imbibition and the fast non-woven fabrics of dispersion as imbibition material, efficiently solve the problem of the slow and easy flow measurement of rate of liquid aspiration like this.It is that gel structure is good that microorganism growth forms condition needed for macroscopic bacterium colony, nutrition is abundant, culture environment is suitable, the cold water that the present invention adopts can be that coldwater-soluble gel that liquid absorption is large and liquid absorption are fast and the ratio easily forming the coldwater-soluble gel of network structure is the mixture of 1:2-1:10 by gel, and adopt the mode of pre-formed gel, namely liquid nutrient medium drips in coldwater-soluble gel formation gel, the mode of drying again, the gel structure that this kind of mode is formed is good, liquid nutrient medium is 2 ×-4 × substratum branch drip 2-5mL uniformly, cultivate base and be at least 7cm × 7cm.This invention ensure that microorganism growth desired nutritional, and distribution of culture medium is even, is easy to formation rule bacterium colony, avoids bacterium colony to trail.Label of the present invention adopts the waterproof color adhesive tape that can write, this invention is easy to distinguish different microorganism test-papers, operator can do experimental record above colored stickers, and as data such as sample number, detection time, detection numberings, this invention is easy to experimenter's operation.
The present invention also provides the using method of microbial rapid detection testing plate described above, specific as follows:
(1) test-paper is lain against on the table top of Bechtop, with sterilizing pipettor pipette samples stoste or diluent 1mL, uncover epiphragma, drip on non-woven fabrics, slowly cover epiphragma.
(2) no more than 12 test-papers are loaded on a valve bag, lie against the incubator of suitable temp.
(3), after cultivating the specified time, colony number is judged according to colony colour.
Microbial rapid detection testing plate of the present invention, alternative traditional sensing techniques, substantially increases microorganism detection efficiency.
Microbial rapid detection testing plate of the present invention ready access upon use, requisite space is few, saves experimenter and configures substratum, the cleaning operation such as glassware, liquid waste disposal, and that strengthens label can write area, experimenter's operation of being more convenient for.
The gel that microbial rapid detection testing plate of the present invention adopts, substratum, label preparation method make microbial rapid detection more accurately, operation is simpler, Detection results is more excellent, homogeneity of product is better.
Accompanying drawing explanation
The product figure of a kind of microbial rapid detection testing plate of Fig. 1.
The base plate figure of the printed on both sides of Fig. 2 microbial rapid detection testing plate.
The cultivation base stereographic map of Fig. 3 microbial rapid detection testing plate.
The transparent film dimensional printed chart of Fig. 4 microbial rapid detection testing plate.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1: the making method of total number of bacterial colony Quick Measuring test piece
Take sodium carboxymethylcellulose pyce 2g, xanthan gum 10g, mix, evenly stick the one side in double faced adhesive tape, cover layer of non-woven fabric above, cut into the square of 7cm × 7cm, be affixed on company out of the press mark and square egative film (9cm × 9cm) middle.Join 4 × total number of bacterial colony substratum fractional point evenly drip with non-woven fabrics above, square to dry.Film (9cm × 9cm) is fixed on base plate with the waterproof color adhesive tape (1.5cm is wide) that can write, the opposite side of film fixed edge has more the 0.5cm on base plate limit.Ready-made Quick Measuring test piece is loaded in valve bag, first open-ended, adopts 50 DEG C of gaseous sterilization 5h.Seal mouth and be placed on Refrigerator store.
Embodiment 2: the test of total number of bacterial colony Quick Measuring test piece
Getting several bacterial strains diluent is inoculated in the test piece of total number of bacterial colony Quick Measuring, and inoculum size is 1mL, and cultivate 15h for 37 DEG C, all bacterial strains all present red colonies.
Embodiment 3: the making method of the quick testing plate of intestinal bacteria
Take ethyl cellulose sodium 2g, guar gum 5g, xanthan gum 5g, Viscogum BE 5g, mixes, and evenly sticks the one side in double faced adhesive tape, cover layer of non-woven fabric above, cut into the square of 8cm × 8cm, be affixed on company out of the press mark and square egative film (10cm × 10cm) middle.Join 2 × Escherichia coli culture medium fractional point evenly drip with non-woven fabrics above, square to dry.Film (10cm × 10cm) is fixed on base plate with the waterproof color adhesive tape (1.5cm is wide) that can write, the opposite side of film fixed edge has more the 0.5cm on base plate limit.Ready-made Quick Measuring test piece is loaded in valve bag, first open-ended, adopts 50 DEG C of gaseous sterilization 5h.Seal mouth and be placed on Refrigerator store.
Embodiment 4: the test of the quick testing plate of intestinal bacteria
Getting several bacterial strains diluent is inoculated in the test piece of total number of bacterial colony Quick Measuring, and inoculum size is 1mL, cultivates 15h for 37 DEG C, and intestinal bacteria all present blue to purple bacterium colony, and other bacterium colonies are colourless or pink.
Claims (9)
1. a making for microbial rapid detection testing plate, comprises the following steps:
(1) the making material requested of microbial rapid detection testing plate: base plate, double faced adhesive tape, non-woven fabrics, transparent film, label, coldwater-soluble gel, substratum;
(2) coldwater-soluble gel evenly sticks the one side in double faced adhesive tape;
(3) another side of coldwater-soluble gel is had to cover layer of non-woven fabric glutinous;
(4) double faced adhesive tape of coldwater-soluble gel is had in the centre detecting egative film by glutinous;
(5) above non-woven fabrics, drip substratum, dry;
(6) cover layer of transparent film above, be fixed on egative film with label;
(7) pack, sterilizing.
2. the making of a kind of microbial rapid detection testing plate according to claim 1, it is characterized in that: baseboard material is enamelled paper or synthetic materials, size is not less than 9cm × 9cm, and one side is printed on company's mark, and the middle of another side is printed on the grid being not less than 7cm × 7cm size.
3. the making of a kind of microbial rapid detection test-paper according to claim 1, is characterized in that: the size in substratum region is not less than 7cm × 7cm..
4. the making of a kind of microbial rapid detection testing plate according to claim 1, is characterized in that: the material of transparent film is film, and top is printed on the graticule line of 1cm × 1cm.
5. the making of a kind of microbial rapid detection testing plate according to claim 1, is characterized in that: the color of label is for monochromatic, and distinct colors represents different microorganism test-papers.
6. the making of a kind of microbial rapid detection testing plate according to claim 1, is characterized in that: coldwater-soluble gel to consist of the large coldwater-soluble gel of liquid absorption and liquid absorption fast and easily form the coldwater-soluble gel of gel network structure.
7. the making of a kind of microbial rapid detection testing plate according to claim 1, it is characterized in that: the coldwater-soluble gel that in the composition of coldwater-soluble gel, liquid absorption is large: liquid absorption is fast and ratio that is the easily coldwater-soluble gel of formation gel network structure is 1:2-1:10, gel proportions in the coldwater-soluble gel that wherein liquid absorption is large is any, and liquid absorption is fast and gel proportions that is that easily formed in the coldwater-soluble gel of gel network structure is any.
8. the making of a kind of microbial rapid detection testing plate according to claim 1, it is characterized in that: the coldwater-soluble gel that liquid absorption is large is ethyl cellulose sodium, sodium carboxymethylcellulose pyce, sodium polyacrylate isogel, liquid absorption is fast and coldwater-soluble gel that is easily formation gel network structure is xanthan gum, guar gum, Viscogum BE isogel.
9. the making of a kind of microbial rapid detection testing plate according to claim 1, is characterized in that: the substratum concentration dripped above non-woven fabrics is 2 ×-4 × and, consumption is 2mL-5mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510294331.5A CN104988203A (en) | 2015-06-02 | 2015-06-02 | Method for making quick microorganism detection test sheet |
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| CN201510294331.5A CN104988203A (en) | 2015-06-02 | 2015-06-02 | Method for making quick microorganism detection test sheet |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754849A (en) * | 2016-03-31 | 2016-07-13 | 吉林农业大学 | Total bacterial count testing slip and preparation method and application thereof |
| CN106854620A (en) * | 2017-01-22 | 2017-06-16 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of rapidly and efficiently detection total plate count and preparation method thereof |
| CN106868092A (en) * | 2016-12-29 | 2017-06-20 | 贵州科学院 | A kind of method of rural potable water microorganism field quick detection |
| CN106906131A (en) * | 2017-03-10 | 2017-06-30 | 西华大学 | Total bacterial count rapid detection card and use method thereof |
| CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
| CN112724474A (en) * | 2020-12-25 | 2021-04-30 | 深圳市航天食品分析测试中心有限公司 | Cold hydrogel and application thereof |
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| US4565783A (en) * | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
| CN101570730A (en) * | 2009-05-27 | 2009-11-04 | 广东省微生物研究所 | Cold water coagulable culture medium coagulator and preparation method thereof |
| CN102363802A (en) * | 2011-10-08 | 2012-02-29 | 广州绿洲生化科技有限公司 | Vibrio parahaemolyticus chromogenic medium and rapid detection card for the same |
-
2015
- 2015-06-02 CN CN201510294331.5A patent/CN104988203A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4565783A (en) * | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
| CN101570730A (en) * | 2009-05-27 | 2009-11-04 | 广东省微生物研究所 | Cold water coagulable culture medium coagulator and preparation method thereof |
| CN102363802A (en) * | 2011-10-08 | 2012-02-29 | 广州绿洲生化科技有限公司 | Vibrio parahaemolyticus chromogenic medium and rapid detection card for the same |
Non-Patent Citations (2)
| Title |
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| 吴毓薇 等: "食品微生物测试片检测技术进展", 《中国卫生检验杂志》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754849A (en) * | 2016-03-31 | 2016-07-13 | 吉林农业大学 | Total bacterial count testing slip and preparation method and application thereof |
| CN105754849B (en) * | 2016-03-31 | 2018-02-16 | 吉林农业大学 | A kind of total plate count test piece, preparation method and applications |
| CN106868092A (en) * | 2016-12-29 | 2017-06-20 | 贵州科学院 | A kind of method of rural potable water microorganism field quick detection |
| CN106854620A (en) * | 2017-01-22 | 2017-06-16 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of rapidly and efficiently detection total plate count and preparation method thereof |
| CN106906131A (en) * | 2017-03-10 | 2017-06-30 | 西华大学 | Total bacterial count rapid detection card and use method thereof |
| CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
| CN112724474A (en) * | 2020-12-25 | 2021-04-30 | 深圳市航天食品分析测试中心有限公司 | Cold hydrogel and application thereof |
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