CN111257234A - Microorganism concentration measuring method - Google Patents
Microorganism concentration measuring method Download PDFInfo
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- CN111257234A CN111257234A CN202010218847.2A CN202010218847A CN111257234A CN 111257234 A CN111257234 A CN 111257234A CN 202010218847 A CN202010218847 A CN 202010218847A CN 111257234 A CN111257234 A CN 111257234A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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Abstract
The invention discloses a method for measuring microbial concentration, which comprises the following steps: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: and respectively putting the LED lamp and the optical signal receiver into microorganism sample solutions with different concentrations. The invention can carry out real-time measurement on the microorganisms by adopting the LED lamp and the optical signal receiver to detect the numerical values of microorganism sample solutions with different concentrations at different temperatures; the operation is simple, the device can be used skillfully without professional skills, the risk that the scientific research personnel easily infect mixed bacteria after manual multiple operations is avoided, and the use of people is facilitated.
Description
Technical Field
The invention relates to the technical field of microorganism measurement, in particular to a microorganism concentration measurement method.
Background
The microorganisms include: the large biological population including bacteria, viruses, fungi, some small protists, microscopic algae and the like has tiny individuals and close relationship with human beings, covers a plurality of beneficial and harmful varieties, widely relates to a plurality of fields such as food, medicine, industry and agriculture, environmental protection, sports and the like, and in the textbooks of the continental china and taiwan, the microorganisms are divided into the following 8 types: bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, spirochetes, some microorganisms are visible to the naked eye like mushrooms, ganoderma, mushrooms, etc. belonging to fungi, and also microorganisms are a class of "non-cellular organisms" consisting of a few components like nucleic acids and proteins, but whose survival must depend on living cells.
In bioengineering or fermentation engineering, often will inoculate a certain amount of microorganism in liquid culture medium, then keep it to cultivate in certain specific environment, in order to monitor the growth state of microorganism in the culture medium, usually need to detect the concentration of microorganism through methods such as optical density measurement, the most common optical density measurement method in laboratory at present is that some samples are taken out by the researcher hand, carry out off-line detection with optical density detector, this kind of microorganism concentration measurement method, need the researcher hand to take a sample many times, complex operation and easy infectious microbe, be unfavorable for people's use.
Disclosure of Invention
The invention aims to provide a microorganism concentration measuring method, which has the advantage of convenient use and solves the problems of complicated manual sampling and detecting operation and easy risk of infecting mixed bacteria in the microorganism concentration measuring method.
In order to achieve the purpose, the invention provides the following technical scheme: a method of measuring a concentration of a microorganism, comprising the steps of:
step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations;
step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value;
and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations;
and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures;
and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Preferably, the stirring device in step 1 is a magnetic ceramic rod with an N pole and an S pole, and can be driven to rotate by an external rotating magnetic field, so as to realize non-contact rapid stirring.
Preferably, the LED lamp and the optical signal receiver in step 3 may be either direct transmission type or reflection type, the optical path may be implemented by optical fiber guiding and mirror reflection, and the power supply of the LED lamp and the optical signal receiver is an internal battery.
Preferably, the temperature sensor in step 3 is an S18B20 temperature sensor, the heating device is heated by a heating plate, and the heating device is insulated by a thermal insulation material.
Preferably, in step 3, both the LED lamp and the optical signal receiver are subjected to waterproof treatment, the LED lamp and the optical signal receiver are made of high-temperature-resistant materials, and the LED lamp and the optical signal receiver are designed in a wireless manner.
Preferably, the temperature of the solution of the microorganism samples with different concentrations in the laboratory in step 2 is maintained at 37 ℃.
Preferably, the time taken for the different concentrations of the microorganism sample solution to rise every 5 ℃ in step 3 is the same.
Compared with the prior art, the invention has the following beneficial effects: the invention can carry out real-time measurement on the microorganisms by adopting the LED lamp and the optical signal receiver to detect the numerical values of microorganism sample solutions with different concentrations at different temperatures; the operation is simple, the device can be used skillfully without professional skills, the risk that the scientific research personnel easily infect mixed bacteria after manual multiple operations is avoided, and the use of people is facilitated.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A method of measuring a concentration of a microorganism, comprising the steps of:
step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations;
step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value;
and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations;
and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures;
and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 1
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 2
In example 1, the following additional steps were added:
in the step 1, the stirring device is a magnetic ceramic rod with an N pole and an S pole, and can be driven to rotate by an external rotating magnetic field, so that non-contact rapid stirring is realized.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 3
In example 2, the following steps were added:
in step 3, the LED lamp and the optical signal receiver may be in a direct transmission type or a reflection type, the optical path may be implemented by means of optical fiber guiding and mirror reflection, and the power supply mode of the LED lamp and the optical signal receiver is an internal battery.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 4
In example 3, the following steps were added:
in the step 3, the temperature sensor is an S18B20 temperature sensor, the heating device is heated by a heating plate, and the heating device is insulated by a heat-insulating material.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 5
In example 4, the following steps were added:
and 3, performing waterproof treatment on the LED lamp and the optical signal receiver, wherein the LED lamp and the optical signal receiver are made of high-temperature-resistant materials and are designed in a wireless mode.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 6
In example 5, the following steps were added:
the temperature of the different concentrations of the microbial sample solutions in the laboratory was maintained at 37 ℃ in step 2.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
Example 7
In example 6, the following additional steps were added:
the time taken for each 5 ℃ rise of the different concentrations of the microbial sample solution in step 3 was the same.
A method of measuring a concentration of a microorganism, comprising the steps of: step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations; step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value; and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations; and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures; and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
In summary, the following steps: the microorganism concentration measuring method solves the problems of complicated manual sampling and detecting operation and easy risk of infecting mixed bacteria in the microorganism concentration measuring method.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A method for measuring a concentration of a microorganism, characterized by: the method comprises the following steps:
step 1: placing microorganisms in a culture dish filled with culture solution, and stirring the culture solution in the culture dish through a stirring device to promote the growth of the microorganisms, so as to prepare microorganism sample solutions with different concentrations;
step 2: placing microorganism sample solutions with different concentrations in a constant-temperature laboratory, and keeping the temperature of the microorganism sample solutions with different concentrations at a certain value;
and step 3: respectively placing an LED lamp and an optical signal receiver into microorganism sample solutions with different concentrations, forming a certain optical path between the LED lamp and the optical signal receiver, enabling the optical path to penetrate through the microorganism sample solutions, detecting optical density values of the microorganism sample solutions with different concentrations by the LED lamp and the optical signal receiver, controlling a heating device by a temperature sensor, continuously increasing the temperature of the microorganism sample solutions with different concentrations, detecting the optical density value once at 5 ℃ per liter, and detecting for 5 times in total to obtain a change curve of the optical density value of the microorganism sample solution with the temperature under different concentrations;
and 4, step 4: respectively taking the optical density values and the temperatures of the obtained microbial sample solutions with different concentrations as an X axis and a Y axis, and establishing the change curves of the optical density values of the microbial sample solutions with different concentrations along with the temperatures;
and 5: the method comprises the steps of putting a microorganism sample solution with unknown concentration into a culture dish filled with a culture solution for culture, controlling a heating device to heat the solution in the culture dish through a temperature sensor, obtaining the concentration of the microorganism sample solution with unknown concentration by detecting the optical density value of the microorganism sample solution, and sending the measured microorganism concentration data to an external person for checking, thereby realizing the measurement of the microorganism concentration.
2. A method of measuring a concentration of a microorganism according to claim 1, wherein: the stirring device in the step 1 is a magnetic ceramic rod with an N pole and an S pole, and can be driven to rotate by an external rotating magnetic field, so that non-contact rapid stirring is realized.
3. A method of measuring a concentration of a microorganism according to claim 1, wherein: in step 3, the LED lamp and the optical signal receiver may be in a direct transmission type or a reflection type, the optical path may be implemented by optical fiber guiding and mirror reflection, and the power supply mode of the LED lamp and the optical signal receiver is an internal battery.
4. A method of measuring a concentration of a microorganism according to claim 1, wherein: the temperature sensor in the step 3 is an S18B20 temperature sensor, the heating device is heated by a heating sheet, and the heating device is insulated by a heat-insulating material.
5. A method of measuring a concentration of a microorganism according to claim 1, wherein: and in the step 3, both the LED lamp and the optical signal receiver are subjected to waterproof treatment, the LED lamp and the optical signal receiver are made of high-temperature-resistant materials, and the LED lamp and the optical signal receiver are designed in a wireless mode.
6. A method of measuring a concentration of a microorganism according to claim 1, wherein: the temperature of the solutions of the different concentrations of the microbiological samples in the laboratory in step 2 was maintained at 37 ℃.
7. A method of measuring a concentration of a microorganism according to claim 1, wherein: the time taken for the different concentrations of the microbial sample solution to rise every 5 ℃ in step 3 is the same.
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| 徐静 等著: "《基于供应链视角的生鲜农产品有效供给保障研究》", 30 November 2017, 江苏大学出版社 * |
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| CN113186082A (en) * | 2021-04-01 | 2021-07-30 | 周文龙 | Microorganism detection system |
| CN114235685A (en) * | 2021-11-03 | 2022-03-25 | 广州先进技术研究所 | Shake flask cultured biomass on-line measuring device |
| CN114235685B (en) * | 2021-11-03 | 2023-08-08 | 广州先进技术研究所 | On-line biomass detection device for shake flask culture |
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