CN108064338A - A kind of immersion type biomass on-line measuring device - Google Patents
A kind of immersion type biomass on-line measuring device Download PDFInfo
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- CN108064338A CN108064338A CN201780001649.7A CN201780001649A CN108064338A CN 108064338 A CN108064338 A CN 108064338A CN 201780001649 A CN201780001649 A CN 201780001649A CN 108064338 A CN108064338 A CN 108064338A
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- light source
- optical signal
- control circuit
- measuring device
- biomass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of immersion type biomass on-line measuring device, including light source, optical signal receiver, control circuit board and waterproof case；Light source, optical signal receiver and control circuit board are all encapsulated in waterproof case, control circuit board respectively with light source, optical signal receiver is electrically connected, waterproof case realizes the protection to internal component, certain light path is formed between light source and optical signal receiver, light path passes through sample, light source and optical signal receiver detect the biomass of sample through two windows on waterproof case by detecting OD value, control circuit board receives external control signal and realizes relevant control function, and the biomass measured is sent to external staff and is checked, so as to fulfill the on-line checking of microorganism growth.The present invention can realize the on-line checking of biomass, avoid troublesome operation and the risk for easily infecting miscellaneous bacteria that off-line measurement method manual sampling detection is brought；Simultaneously suitable for the culture dish or fermentation tank of arbitrary shape.
Description
Technical field
The present invention relates to Bioexperiment equipment technical fields, and in particular to a kind of immersion type biomass on-line measuring device.
Background technology
In bioengineering or Fermentation Engineering, often a certain amount of microorganism of inoculation in liquid medium within, then will
It is stored in some specific environment and cultivates.In order to monitor the growth conditions of microorganism in culture medium, it usually needs pass through light
The methods of density (OD) measurement, is detected.
The most common optical density in laboratory (OD) measuring method at present is to manually take out a part of sample by scientific research personnel,
Optical density (OD) detector carries out offline inspection.
CN105044038A discloses a kind of non-intrusion type biomass on-line measuring device for Shaking culture, the device
Including main control unit, the shaking flask of biological suspension can be accommodated and cover on the fixing sleeve on the outside of shaking flask.The device can be realized to shaking
The concentration of biological suspension carries out on-line checking in bottle.
Most common offline optical density (OD) measuring method in laboratory is, it is necessary to scientific research personnel repeatedly samples manually at present, behaviour
Make cumbersome and easily infect miscellaneous bacteria.
A kind of non-intrusion type biomass on-line measuring device for Shaking culture disclosed in CN105044038A, due to having
One special fixing sleeve covered on the outside of shaking flask, thus a device is only applicable to the shaking flask of given shape size, versatility
Difference.
The content of the invention
In view of this, in order to solve the above problem of the prior art, the present invention is directed to propose a kind of immersion type biomass
On-line measuring device.The device can realize the on-line checking of biomass, and off-line measurement method manual sampling detection is avoided to bring
Troublesome operation and easily infection miscellaneous bacteria risk；The device is suitable for the culture dish or fermentation tank of arbitrary shape simultaneously, only
It need to place a device into culture dish or fermentation tank, can be achieved with the on-line checking to internal biomass.
The present invention is solved the above problems by following technological means：
On the one hand, the present invention provides a kind of immersion type biomass on-line measuring device, including light source, optical signal receiver,
Control circuit board and waterproof case；Light source, optical signal receiver and control circuit board are all encapsulated in waterproof case, control circuit
Plate is electrically connected respectively with light source, optical signal receiver, and waterproof case realizes the protection to internal component, and light source and optical signal connect
Certain light path is formed between receipts device, light path passes through sample, light source and optical signal receiver through two on waterproof case
A window detects the biomass of sample by detecting OD value, and control circuit board receives external control signal and realizes correlation
Control function, and the biomass measured is sent to external staff and is checked, so as to fulfill the on-line checking of microorganism growth.
Further, the immersion type biomass on-line measuring device is using internal battery powers or external using wired
Power supply is powered；When using internal battery powers, internal cell is further included, internal cell is encapsulated in waterproof case, internal electricity
Pond is integrated in control circuit board and is electrically connected with control circuit board.
Further, the immersion type biomass on-line measuring device using transmission of wireless signals mode or uses wired letter
Number transmission mode；When using transmission of wireless signals mode, wireless signal transmitting unit, wireless signal transmitting unit envelope are further included
In waterproof case, wireless signal transmitting unit is integrated in control circuit board and is electrically connected with control circuit board.
Further, the wireless signal transmitting unit is Wifi modules, RFID module or bluetooth module.
Further, the light path of light source and optical signal receiver uses direct delivery type or reflective.
Further, the waterproof case is spheroid shape, cross or cylinder.
Further, the waterproof case uses the waterproof type of sealing ring, fluid sealant, thread seal or interference fit.
Further, the light source uses LED and laser diode, the optical signal receiver using photodiode or
Phototriode.
Further, the immersion type biomass on-line measuring device further includes stirring rod, and stirring rod is a kind of with N poles
With the magnetism stick of S poles, stirring rod is encapsulated in waterproof case, drives its rotation entire so as to drive by the magnetic field of outside rotation
Device rotates, and realizes the purpose of stirring.
On the other hand, the present invention also provides a kind of immersion type biomass on-line measuring device, received including light source, optical signal
Device, control circuit board；Light source, optical signal receiver and control circuit board all carry out water-proofing treatment, control circuit board respectively with light
Source, optical signal receiver electrical connection, form certain light path between light source and optical signal receiver, light path passes through sample,
Light source and optical signal receiver detect the biomass of sample by detecting OD value, and control circuit board receives external control
Signal realizes relevant control function, and the biomass measured is sent to external staff and is checked, is given birth to so as to fulfill microorganism
Long on-line checking.
Compared with prior art, beneficial effects of the present invention are as follows：
1), realize to the biomass on-line checking in culture solution, avoid the troublesome operation of manual sampling and sampled
The risk of miscellaneous bacteria is infected in journey；
2), suitable for the culture dish or fermentation tank of arbitrary shape, need to only place a device into culture dish or fermentation tank, with regard to energy
Realize the on-line checking to biomass in culture solution；
3), all components can be encapsulated in a waterproof case, compact in structure and convenient to use；
4), can be combined with other Bioexperiment common tools, it is versatile.
Description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some embodiments of the present invention, for
For those of ordinary skill in the art, without creative efforts, other are can also be obtained according to these attached drawings
Attached drawing.
Fig. 1 is 1 usage scenario schematic diagram of immersion type biomass on-line measuring device embodiment of the present invention；
Fig. 2 is 1 wireless type structure diagram of immersion type biomass on-line measuring device embodiment of the present invention (based on wherein A
View complete section, B are explosive view)；
Fig. 3 is 1 wired structure diagram of immersion type biomass on-line measuring device embodiment of the present invention (based on wherein A
View complete section, B are explosive view)；
Fig. 4 is that immersion type biomass on-line measuring device embodiment 1 of the present invention is combined formula design diagram with stirring rod
(wherein A is front view complete section, and B is explosive view)；
Fig. 5 is 1 several specific light path schematic diagrams of immersion type biomass on-line measuring device embodiment of the present invention；
Fig. 6 is 1 several specific outline structural diagrams of immersion type biomass on-line measuring device embodiment of the present invention；
Reference sign：
1st, waterproof case, 2, light source, 3, optical signal receiver, 4, control circuit board, 5, stirring rod, 10, culture dish, 20,
Culture solution, 30, immersion type biomass on-line measuring device.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below in conjunction with attached drawing and specifically
Embodiment technical scheme is described in detail.It is pointed out that described embodiment is only this hair
Bright part of the embodiment, instead of all the embodiments, based on the embodiments of the present invention, those of ordinary skill in the art are not having
All other embodiments obtained under the premise of creative work are made, belong to the scope of protection of the invention.
Embodiment 1
The present invention is directed to propose a kind of immersion type biomass on-line measuring device, which detects without manual sampling, allows
Scientific research personnel frees from frequent manual operation, has been greatly reduced the risk of manual operation infection miscellaneous bacteria.It makes
With scene as shown in Figure 1, (in figure：10th, culture dish, 20, culture solution, 30, immersion type biomass on-line measuring device).
Immersion type biomass on-line measuring device 30 of the present invention mainly includes light source 2, optical signal receiver 3, control circuit
Plate 4 and waterproof case 1.Light source 2, optical signal receiver 3 and control circuit board 4 are all encapsulated in waterproof case 1, control circuit board
4 are electrically connected respectively with light source 2, optical signal receiver 3.
Light source 2 and optical signal receiver 3 realize the measurement to sample, and control circuit board 4 realizes relevant control function,
Waterproof case 1 realizes the protection to internal component.
Certain light path is formed between light source 2 and optical signal receiver 3, light path has to pass through sample.
Light source 2 and optical signal receiver 3 are through two windows detecting samples on waterproof case 1.Setting is every one
It fixes time and is detected automatically, so as to fulfill the on-line checking of microorganism growth.
Internal battery powers may be employed in device, wired external power supply can also be used to power.
Device can realize " reception/transmission " of external control signal and data measured, and wireless signal transmission may be employed
Mode (Wifi, RFID, bluetooth etc.), can also be used wire signal transmission mode.
Waterproof case 1 mainly realizes the protective effect to internal component, while waterproof case 1 makes succinct shape,
Attachment of the microorganism on device can be reduced；If doing water-proofing treatment in itself to internal component, waterproof case 1 can also take
Disappear.
LED and laser diode etc. can be used in light source 2, and photodiode or phototriode can be used in optical signal receiver 3
Pipe etc..
Several possible concrete schemes
(A) designed using wireless type
The components such as power supply (internal cell), signal " reception/transmission " device (wireless signal transmitting unit) are fully integrated
In control circuit board 4, inside waterproof case 1, light source 2 and optical signal receiver 3 are through two on waterproof case 1
Windows detecting sample.Dependency structure is as shown in Figure 2.
(B) designed using wired
Using wired external power supply or wired signal transmission form, controlled in a manner that lead is accessed in outside with internal
Circuit board 4 processed connects, and other parts are similar with option A.Dependency structure is as shown in Figure 3.
(C) it is combined with other common tools
In microbial cultivation process is carried out, it is often necessary to use various instruments.For example, it is usually needed during microculture
Culture solution is stirred continuously so that microorganism to be promoted to grow with stirring rod 5, therefore, which can be designed to and 5 phase knot of stirring rod
The mode of conjunction achievees the purpose that measure the biomass in culture solution while agitating.Dependency structure is as shown in figure 4, stirring rod 5
It penetrates control circuit board 4 and is encapsulated in waterproof case 1." penetrating " is one kind in its arrangement, in addition, may be used also
To there is other arrangement, as long as stirring rod 5 can drive whole device normally to rotate.
Stirring rod 5 is a kind of magnetic ceramics stick with N poles and S poles, and size is about 15 × 60mm of Φ, can pass through outside
The magnetic field of rotation drives its rotation, so as to fulfill contactless quick stirring.The present invention is combined stirring rod 5 with detection device,
In whipping process, stirring rod 5 drives whole device rotation, realizes the purpose measured while stirring.
The invention has the characteristics that：
1), when using wired design, power supply may be either that externally fed can be in-line power again.It is set when using wireless type
Timing, power supply power supply mode can be internal installation battery or utilize electromagnetic induction effect etc.；
2), the light path of light source 2 and optical signal receiver 3 is either directly delivery type can be reflective again.Light path can
It is guided by optical fiber, the modes such as mirror-reflection are realized.Several specific paths modes are as shown in Figure 5：(in figure, L
For light source 2, S optical signal receivers 3)
3), the appearance profile of waterproof case 1 can be varied, such as spheroid shape, cross, cylinder etc..Such as Fig. 6 institutes
Show.
4), the various specific waterproof sides such as sealing ring, fluid sealant, thread seal, interference fit may be employed in waterproof case 1
Formula；
If 5), do water-proofing treatment in itself to internal component, waterproof case 1 can also be cancelled.
Embodiment 2
The present invention also provides a kind of immersion type biomass on-line measuring device, including light source, optical signal receiver, control electricity
Road plate；Light source, optical signal receiver and control circuit board all carry out water-proofing treatment, control circuit board respectively with light source, optical signal
Receiver is electrically connected, and certain light path is formed between light source and optical signal receiver, and light path passes through sample, light source and light letter
Number receiver detects the biomass of sample by detecting OD value, and control circuit board receives external control signal and realizes phase
Control function is closed, and the biomass measured is sent to external staff and is checked, so as to fulfill the online inspection of microorganism growth
It surveys.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of immersion type biomass on-line measuring device, which is characterized in that including light source, optical signal receiver, control circuit
Plate and waterproof case；Light source, optical signal receiver and control circuit board are all encapsulated in waterproof case, control circuit board respectively with
Light source, optical signal receiver electrical connection, waterproof case realizes the protection to internal component, between light source and optical signal receiver
Certain light path is formed, light path passes through sample, light source and optical signal receiver to lead to through two windows on waterproof case
The biomass of detection OD value detection sample is crossed, control circuit board receives external control signal and realizes relevant control work(
Can, and the biomass measured is sent to external staff and is checked, so as to fulfill the on-line checking of microorganism growth.
2. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the immersion type biomass
On-line measuring device is using internal battery powers or using the power supply of wired external power supply；When using internal battery powers, also
Including internal cell, internal cell is encapsulated in waterproof case, and internal cell is integrated in control circuit board and and control circuit
Plate is electrically connected.
3. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the immersion type biomass
On-line measuring device is using transmission of wireless signals mode or uses wire signal transmission mode；When using transmission of wireless signals mode
When, wireless signal transmitting unit is further included, wireless signal transmitting unit is encapsulated in waterproof case, wireless signal transmitting unit collection
It is electrically connected into control circuit board and with control circuit board.
4. immersion type biomass on-line measuring device according to claim 3, which is characterized in that the wireless signal transmission
Unit is Wifi modules, RFID module or bluetooth module.
5. immersion type biomass on-line measuring device according to claim 1, which is characterized in that light source is received with optical signal
The light path of device uses direct delivery type or reflective.
6. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the waterproof case is ellipse
Ball-type, cross or cylinder.
7. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the waterproof case uses
Sealing ring, fluid sealant, the waterproof type of thread seal or interference fit.
8. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the light source uses LED
And laser diode, the optical signal receiver use photodiode or phototriode.
9. immersion type biomass on-line measuring device according to claim 1, which is characterized in that the immersion type biomass
On-line measuring device further includes stirring rod, and stirring rod is a kind of magnetism stick with N poles and S poles, and stirring rod is encapsulated in outside waterproof
In shell, by the magnetic field of outside rotation its rotation is driven to realize the purpose of stirring so as to which whole device be driven to rotate.
10. a kind of immersion type biomass on-line measuring device, which is characterized in that including light source, optical signal receiver, control circuit
Plate；Light source, optical signal receiver and control circuit board all carry out water-proofing treatment, and control circuit board connects respectively with light source, optical signal
Device electrical connection is received, certain light path is formed between light source and optical signal receiver, light path passes through sample, light source and optical signal
Receiver detects the biomass of sample by detecting OD value, and control circuit board receives external control signal and realizes correlation
Control function, and the biomass measured is sent to external staff and is checked, so as to fulfill the on-line checking of microorganism growth.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2017/109580 WO2019084969A1 (en) | 2017-11-06 | 2017-11-06 | Immersion biomass on-line detection apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108064338A true CN108064338A (en) | 2018-05-22 |
Family
ID=62141998
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780001649.7A Pending CN108064338A (en) | 2017-11-06 | 2017-11-06 | A kind of immersion type biomass on-line measuring device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108064338A (en) |
| WO (1) | WO2019084969A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110455718A (en) * | 2019-08-16 | 2019-11-15 | 佛山市川东磁电股份有限公司 | A kind of liquid and its turbidity detection device |
| CN111257234A (en) * | 2020-03-25 | 2020-06-09 | 杭州御佳金属材料有限公司 | Microorganism concentration measuring method |
| CN113388488A (en) * | 2020-03-12 | 2021-09-14 | 广州中国科学院先进技术研究所 | Probe type biomass on-line detection device capable of achieving high-temperature sterilization |
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| CN113388488A (en) * | 2020-03-12 | 2021-09-14 | 广州中国科学院先进技术研究所 | Probe type biomass on-line detection device capable of achieving high-temperature sterilization |
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| Publication number | Publication date |
|---|---|
| WO2019084969A1 (en) | 2019-05-09 |
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Application publication date: 20180522 |