CN201034925Y - Hand-hold adenosine triphosphate fluorescent testing apparatus - Google Patents
Hand-hold adenosine triphosphate fluorescent testing apparatus Download PDFInfo
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- CN201034925Y CN201034925Y CNU2007200314767U CN200720031476U CN201034925Y CN 201034925 Y CN201034925 Y CN 201034925Y CN U2007200314767 U CNU2007200314767 U CN U2007200314767U CN 200720031476 U CN200720031476 U CN 200720031476U CN 201034925 Y CN201034925 Y CN 201034925Y
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Abstract
The utility model relates to a hand-held atp fluorescence detection device, including a shell composed by an upper shell and a lower shell, a cover for opening or closing is arranged above the shell, the upper shell is used as the panel of the device, the panel is provided with a liquid crystal window and a keyboard; the inner shell is provided with a circuit board for fluorescence detection, the circuit board is also provided with a lcd screen, a photodiode, a shielding cover and an irradiation metal block, the photodiode is disposed in the irradiation metal block, the other end thereof is connected with a test-tube storehouse, the circuit board is also provided with a first microswitch and a second microswitch. The control circuit includes a fluorescence detection module, a data acquisition module, a man-machine interface, a power supply module and a control module and others. The fluorescence detection module is used to detect the fluorescence intensity produced by the reaction of the ATP in the live microorganism and the fluorescein and the luciferase in the detection reagents, the fluorescence intensity is converted into digital quantity for determining the content of ATP, the control system is used to analysis, treat and store the data, which can indirectly determine the existing and the contents of microorganism.
Description
Technical Field
The utility model relates to a detection device, especially a hand-held type adenosine triphosphate fluorescence detection device, the device can be applied to food safety, and the environmental detection department is used for on-the-spot microorganism short-term test, and the quick examination of big sample reaches key link monitoring, and prevention infectious disease ensures public safety, food safety and environmental monitoring.
Background
Atp (adenosine triphosphate), the name of chinese, is adenosine triphosphate, is present as an important energy molecule in all organisms from microorganisms to higher animals and plants. Since ATP is contained in living microorganisms, the ATP content in a sample can be determined by detecting the fluorescence intensity by means of ATP fluorescence reaction of the following reaction formula, and thus the presence and amount of microorganisms in the sample can be indirectly determined.
LH2□AMP+O2→AMP+CO2+ oxyfluorescein + light (2)
The method has the advantages of broad spectrum, high sensitivity and high speed: the sensitivity can reach 10-13mol, even up to 10 if some links can be controlled-18mol; the traditional petri dish method requires 48 hours for determining the number of bacteria, the method only requires several minutes, and the handheld device only requires ten seconds.
The traditional method is to use desk type equipment for ATP fluorescence detection of microorganisms, and the desk type equipment is large in size and cannot be conveniently and flexibly applied to occasions of field rapid detection due to alternating current power supply.
According to the data retrieval conducted by the applicant, no relevant report is reported on a hand-held ATP fluorescence detector prepared based on an MSP430 ultra-low power consumption microcontroller of the American TI (Texas instruments) company.
Disclosure of Invention
An object of the utility model is to provide an use battery powered hand-held type adenosine triphosphate fluorescence detection device, the device can be at the scene quick detection microorganism in 10s to produce the testing result, and can preserve 1000 testing results, use disposable ATP detect reagent, avoid disturbing.
In order to realize the above functions, the utility model discloses take following technical scheme:
a hand-held adenosine triphosphate fluorescence detection device comprises a shell consisting of an upper shell and a lower shell, and is characterized in that an open/close cover is arranged above the shell, the upper shell is used as a panelof the device, and a liquid crystal window and a keyboard are distributed on the panel; install the circuit board that is used for fluorescence detection in the casing, still install the LCD screen on the fluorescence detection circuit board, photodiode, shield cover and light-resistant metal block, photodiode are located the light-resistant metal block, then the other end is connected with the test tube storehouse, still sets up on the fluorescence detection circuit board to be equipped with first micro-gap switch and second micro-gap switch.
The utility model discloses a hand-held type adenosine triphosphate fluorescence detection device compares current desk-top ATP fluorescence detection equipment, has detectivity height, and quick, portability, low-power consumption and broad-spectrum advantage, application that can be more convenient are to microorganism witnessed inspections. The infectious disease prevention, the public safety, the food safety and the environmental monitoring are ensured.
Drawings
FIG. 1 is a schematic view of the instrument (front side);
FIG. 2 is a schematic view (side) of the instrument;
FIG. 3 is a block diagram of the general circuit structure of the ATP fluorescence detector;
FIG. 4 is an example of a specific circuit of the fluorescence detection module;
fig. 5 is a specific circuit example of the DC/DC module and the soft on/off module.
The reference numerals in fig. 1, 2 denote: 1. the test tube comprises an upper shell, 2, a lower shell, 3, a cover, 4, a liquid crystal screen, 5, a test tube bin, 6, a first microswitch, 7, a second microswitch, 8, a light-resistant metal block, 9, a circuit board, 10, a keyboard, 11, an RS232 interface, 12, a photodiode, 13 and a shielding cover.
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Detailed Description
The utility model adopts the photodiode as the core device of weak ATP fluorescence detection, designs the preamplifier circuit with the characteristics of low noise, high sensitivity and strong anti-interference capability, and is used for detecting the fluorescence signal obtained by the photodiode; the low-power design of the system is realized by using an MSP430 series ultra-low-power microcontroller of American TI company, a power supply circuit with ultra-low static current and leakage current is designed, and two AA batteries are used.
The circuit part of the whole handheld ATP fluorescence detection device comprises a fluorescence detection module, a data acquisition module, a human-computer interaction interface, a power supply module, a control module and the like. The fluorescence detection module comprises a photodiode (selected from Hamamatsu corporation) and a preamplifier circuit with low noise, high sensitivity and strong anti-interference capability based on AD8605 of Analog corporation; the data acquisition module comprises a 12-bit A/D converter internally integrated in the MSP430, an external reference source circuit used by the 12-bit A/D converter and an I-based data storage module for storing acquired data2EEPROM of the C bus; the power supply module comprises two serial AA batteries, a voltage stabilizing module for converting the voltage of the two serial AA batteries into stable 3.3V output and supplying power to the whole system based on a voltage boosting type DC/DC chip SP6641A of Sipex company, and a voltage stabilizing module for converting the 3.3V voltage into stable 3V output and supplying power to the fluorescence detection module based on a low dropout Linear (LDO) chip SPX5205 of the Sipex company.
Referring to fig. 1 and 2, the appearance and internal structure of the ATP fluorescence monitoring device are described. The device comprises a shell consisting of an upper shell 1 and a lower shell 2, wherein an open/close cover 3 is arranged above the shell, the upper shell 1 is used as a panel of the device, and a liquid crystal window and a keyboard 10 are distributed on the panel; install the circuit board 9 that is used for fluorescence detection in the casing, still install LCD screen 4 on the fluorescence detection circuit board 9, photodiode 12, shield cover 13 and light-resistant metal block 8, photodiode are located light-resistant metal block 8, and light-resistant metal block 8 one end is connected with test tube storehouse 5, still sets up on the fluorescence detection circuit board 9 and is equipped with first micro-gap switch 6 and second micro-gap switch 7.
The fluorescence detection circuit board 9 comprises a control system (I), a fluorescence detection and data acquisition module (II), a human-computer interaction interface (III) and a power supply module (IV);
the control system (I) comprises: 12-bit A/D converter, I integrated with microcontroller 242C bus module, USART module, JTAG module, liquid crystal drive control module, interruptible I/OA module; a universal synchronous and asynchronous serial receiver and repeater (USART) module connected to the RS232 interface 11 for on-line communication with a computer, a JTAG (Joint test action group, an international standard test protocol) module connected to the computer via a JTAG interface 23, I2The C modules are respectively provided with an EEPROM (electrically erasable programmable read-Onlymemory) 22 and a real-time clock module 21 for managing time;
the fluorescence detection and data acquisition module (II) is connected to the control system (I) and comprises a fluorescence detection module 20 and a voltage reference source 19 which are respectively communicated with a 12-bit A/D converter of the control system (I); the shielding case 13 covers the fluorescence detection module 20 on the fluorescence detection circuit board 9 and is connected to the ground to shield external electromagnetic interference.
The human-computer interaction interface (III) is also connected to the control system (I) and comprises a first microswitch 6, a second microswitch 7, a keyboard 10, a buzzer 14 and a liquid crystal screen 4, wherein the first microswitch 6, the second microswitch 7 and the keyboard 10 are communicated with an interruptible I/O module of the control system (I), and the liquid crystal screen 4 is communicated with a liquid crystal driving control module of the control system (I);
the power supply module (IV) is used for powering the device and comprises an interconnecting battery 16, a DC/DC converter 17 and an LDO chip 18.
The keypad 10 is a membrane keypad.
The LDO chip 18 is used for converting the output voltage of the DC/DC chip into a higher-precision voltage to supply power for the fluorescence detection module.
The first microswitch 6 is a needle-shaped button type microswitch, and the second microswitch 7 is a swing rod type microswitch.
The photodiode 12 is used for converting the current signal of the fluorescence into a voltage signal.
The RS232 interface 11 adopts a 4-pin USB socket for the on-line communication with the computer.
The first microswitch 6 and the second microswitch 7 activate the detection means by closure of the lid 3 and insertion of the test tube.
When the cover 3 is closed, the cylindrical bulge on the cover 3 props against the mandril of the micro first switch 6 to trigger the micro first switch; when the test tube bin 5 is inserted into a test tube, the test tube presses down the swing rod of the second microswitch 7 to trigger the test tube. Thereby enabling monitoring of the cap condition and insertion of the test tube. The transparent observation window at the tail end of the test tube bin 5 extends into the light-proof metal block 8, and the fluorescence penetrates through the observation window, passes through the hole in the light-proof metal block 8 and irradiates on the photodiode 12 positioned below the light-proof metal block 8, so that the influence of light leakage can be shielded. The RS232 interface 11 uses a 4 pin Mini USB socket to save limited space.
Referring to fig. 3, a TI corporation MSP430 series microcontroller 24 is employed that integrates a 12-bit a/D converter for data acquisition, a universal synchronous and asynchronous serial receiver and repeater (USART) for communication with a PC, and an I for communication with an EEPROM memory chip 22 and a real time clock module 212A C bus module, a liquid crystal drive control module for driving and controlling the segment liquid crystal, an I/O module with an interrupt function for checking the circuit and system status of the keyboard 10, a JTAG module for online programming and online debugging, etc. All peripheral modules of the whole ATP fluorescence detection system are designed around the microcontroller and the chip and the peripheral devices thereof.
FIG. 4 is a specific circuit embodiment of the fluorescence detection module. The fluorescence detection module detects the fluorescence intensity generated by the reaction ofATP in the living microorganism and luciferin and luciferase in the detection reagent, the fluorescence intensity is converted into digital quantity through the data acquisition module to determine the ATP content, and the control system analyzes, processes and stores the data, thereby indirectly determining the existence and the content of the microorganism. In this embodiment, VCC-3V is the stable and accurate supply voltage provided to the module, and the integrated op-amp U1 provides a bias voltage to the two stage backward amplifier circuit, which is output to the AD conversion module via ADin 2. The first-stage amplification circuit with the U2 as a core converts a current signal of the fluorescent light-irradiating photodiode D1 into a voltage signal, outputs the voltage signal to the second-stage amplification circuit with the U3 as the core, and outputs the amplified signal to the AD conversion module through the ADin 1. R5, R6 and C6 constitute a low-pass filter circuit to eliminate the influence of high-frequency interference and noise.
Fig. 5 is a specific circuit example of the DC/DC module and the soft on/off module. In this example, U1 (slice SP6641), together with the inductor L1, schottky diode D1, and capacitor C1, form a DC/DC power supply module that converts the battery voltage to a 3.3V voltage that is output stably to supply power to the entire system.
The triodes Q1, Q2, Q3, Q4 and a plurality of resistors form a soft on/off circuit, Vbat is the battery voltage, power _ ctr is connected with an output pin of the microcontroller, and power _ off is connected with an interruptible I/O pin of the microcontroller. When the system is in a shutdown state, a Power key is pressed to enable the Q2 to be conducted, then the Q1 is conducted to enable the battery voltage Vbat to be output to the DC/DC module, the DC/DC module is started and outputs 3.3V voltage to be supplied to the microcontroller, the microcontroller is started and outputs high level on a pin connected with the Power _ ctr to enable the Q3 to be conducted, therefore, the conduction state of the Q1 is maintained, and the whole system enters a normal working state. If the Power key is pressed again, the level of a pin connected with Power _ off of the microcontroller is changed from high to low due to the conduction of Q4, so that the microcontroller is powered off and the Power _ ctr is set to low; when the Power key is bounced, Q2 and Q3 are not conducted, so that Q1 is not conducted, the Power supply of the DC/DC module battery is interrupted and the whole device is turned off.
Claims (8)
1. A hand-held adenosine triphosphate fluorescence detection device comprises a shell consisting of an upper shell (1) and a lower shell (2), and is characterized in that an open/close cover (3) is arranged above the shell, the upper shell (1) is used as a panel of the device, and a liquid crystal window and a keyboard (10) are distributed on the panel; install fluorescence detection circuit version (9) in the casing, still install LCD screen (4), photodiode (12), shield cover (13) and light-resistant metal block (8) on fluorescence detection circuit version (9), photodiode (12) are located light-resistant metal block (8), and light-resistant metal block (8) one end is connected with test tube storehouse (5), still sets up on fluorescence detection circuit version (9) and is equipped with first micro-gap switch (6) and second micro-gap switch (7).
2. The hand-held adenosine triphosphate fluorescence detection device according to claim 1, wherein the fluorescence detection circuit board (9) comprises a control system (I), a fluorescence detection and data acquisition module (II), a human-computer interaction interface (III) and a power supply module (IV);
the control system (I) comprises a 12-bit A/D converter and a microcontroller (24) which are integrated into a whole2The system comprises a C bus module, a universal synchronous and asynchronous serial receiver and transponder module, a JTAG module, a liquid crystal drive control module and an interruptible I/O module;
the universal synchronous and asynchronous serial receiver and repeater are connected with an RS232 interface (11) for on-line communication with a computer, the JTAG module is connected with the computer through a JTAG interface (23), I2The C modules are respectively provided with a memory EEPROM (22) and a real-time clock module (21) for managing time;
the fluorescence detection and data acquisition module (II) is connected to the control system (I) and comprises a fluorescence detection module (20) and a voltage reference source (19), which are respectively communicated with a 12-bit A/D converter of the control system (I); wherein the upper part of the fluorescence detection module (20) is covered by a shielding cover (13) connected with the ground so as to shield external electromagnetic interference;
the human-computer interaction interface (III) is also connected to the control system (I) and comprises a first microswitch (6), a second microswitch (7), a keyboard (10), a buzzer (14) and a liquid crystal screen (4), wherein the first microswitch (6), the second microswitch (7) and the keyboard (10) are communicated with an interruptible I/O module of the control system (I), and the liquid crystal screen (4) is communicated with a liquid crystal driving control module of the control system (I);
the power supply module (IV) is used for supplying power to the device and comprises a battery (16), a DC/DC converter (17) and a low-dropout linear voltage stabilizing chip (18) which are connected with each other.
3. The hand-held fluorescent adenosine triphosphate detection device according to claim 1 or 2, wherein the keyboard (10) is a membrane keyboard.
4. The hand-held adenosine triphosphate fluorescence detection device according to claim 2, wherein the low dropout linear regulator chip (18) is used for converting the output voltage of the DC/DC chip into a higher precision voltage to supply power to the fluorescence detection module.
5. The hand-held adenosine triphosphate fluorescence detection device according to claim 2, wherein the first microswitch (6) is a needle button microswitch, and the second microswitch (7) is a rocker microswitch.
6. The hand-held fluorescent adenosine triphosphate detection device according to claim 1 or 2, wherein the photodiode (12) is used to convert a current signal of the fluorescence into a voltage signal.
7. The hand-held fluorescence adenosine triphosphate detection device of claim 2, wherein the RS232 interface (11) communicates with a computer on-line using a 4-pin USB socket.
8. The hand-held fluorescent detection device for adenosine triphosphate according to claim 1 or 5, characterized in that the first microswitch (6) and the second microswitch (7) activate the detection device by closing the lid (3) and inserting the cuvette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200314767U CN201034925Y (en) | 2007-04-03 | 2007-04-03 | Hand-hold adenosine triphosphate fluorescent testing apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200314767U CN201034925Y (en) | 2007-04-03 | 2007-04-03 | Hand-hold adenosine triphosphate fluorescent testing apparatus |
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| CN201034925Y true CN201034925Y (en) | 2008-03-12 |
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| CNU2007200314767U Expired - Lifetime CN201034925Y (en) | 2007-04-03 | 2007-04-03 | Hand-hold adenosine triphosphate fluorescent testing apparatus |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676381A (en) * | 2011-03-18 | 2012-09-19 | 上海华勤通讯技术有限公司 | Mobile terminal with bacteria detection function |
| CN103305407A (en) * | 2013-06-06 | 2013-09-18 | 南宁市健雄厨房设备有限公司 | Kitchen appliance bacteria detection device |
| CN103810826A (en) * | 2014-01-07 | 2014-05-21 | 上海朗赋实业有限公司 | Wireless ATP (Adenosine Triphosphate) detector |
| CN104970780A (en) * | 2015-07-13 | 2015-10-14 | 上海斐讯数据通信技术有限公司 | Influenza detecting system and method based on mobile terminal |
| CN105466914A (en) * | 2015-11-24 | 2016-04-06 | 西安天隆科技有限公司 | High-performance bioluminescence detection device |
| CN106841040A (en) * | 2017-03-28 | 2017-06-13 | 四川卫康科技有限公司 | Microorganism detection system and method |
| CN107164564A (en) * | 2017-07-05 | 2017-09-15 | 新疆畜牧科学院兽医研究所（新疆畜牧科学院动物临床医学研究中心） | One kind is used to detect canine parainfluenza virus kit |
| CN109097265A (en) * | 2018-08-08 | 2018-12-28 | 徐莹 | A kind of microbial detection device |
| CN110907440A (en) * | 2019-12-02 | 2020-03-24 | 陕西瑞奇生物科技有限公司 | Rapid quantitative detection method for sulfur dioxide |
| CN112147131A (en) * | 2020-08-20 | 2020-12-29 | 西安交通大学 | Portable microorganism short-term test appearance |
| WO2023029053A1 (en) * | 2021-09-06 | 2023-03-09 | 皮乐迪有限公司 | Test device |
-
2007
- 2007-04-03 CN CNU2007200314767U patent/CN201034925Y/en not_active Expired - Lifetime
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676381A (en) * | 2011-03-18 | 2012-09-19 | 上海华勤通讯技术有限公司 | Mobile terminal with bacteria detection function |
| CN103305407A (en) * | 2013-06-06 | 2013-09-18 | 南宁市健雄厨房设备有限公司 | Kitchen appliance bacteria detection device |
| CN103810826A (en) * | 2014-01-07 | 2014-05-21 | 上海朗赋实业有限公司 | Wireless ATP (Adenosine Triphosphate) detector |
| CN104970780A (en) * | 2015-07-13 | 2015-10-14 | 上海斐讯数据通信技术有限公司 | Influenza detecting system and method based on mobile terminal |
| CN105466914A (en) * | 2015-11-24 | 2016-04-06 | 西安天隆科技有限公司 | High-performance bioluminescence detection device |
| CN106841040A (en) * | 2017-03-28 | 2017-06-13 | 四川卫康科技有限公司 | Microorganism detection system and method |
| CN107164564A (en) * | 2017-07-05 | 2017-09-15 | 新疆畜牧科学院兽医研究所（新疆畜牧科学院动物临床医学研究中心） | One kind is used to detect canine parainfluenza virus kit |
| CN109097265A (en) * | 2018-08-08 | 2018-12-28 | 徐莹 | A kind of microbial detection device |
| CN109097265B (en) * | 2018-08-08 | 2022-01-11 | 徐莹 | Microorganism detection device |
| CN110907440A (en) * | 2019-12-02 | 2020-03-24 | 陕西瑞奇生物科技有限公司 | Rapid quantitative detection method for sulfur dioxide |
| CN112147131A (en) * | 2020-08-20 | 2020-12-29 | 西安交通大学 | Portable microorganism short-term test appearance |
| WO2023029053A1 (en) * | 2021-09-06 | 2023-03-09 | 皮乐迪有限公司 | Test device |
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Effective date of abandoning: 20070403 |
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| C25 | Abandonment of patent right or utility model to avoid double patenting |