CN106841040A - Microorganism detection system and method - Google Patents
Microorganism detection system and method Download PDFInfo
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- CN106841040A CN106841040A CN201710190683.5A CN201710190683A CN106841040A CN 106841040 A CN106841040 A CN 106841040A CN 201710190683 A CN201710190683 A CN 201710190683A CN 106841040 A CN106841040 A CN 106841040A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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Abstract
The present invention relates to microorganism detection field, in particular to a kind of microorganism detection system and method.The microorganism detection system, under the collaborative work of control device and emission light source device, fluorescence reception device and microbial culture medium apparatus for placing, realizes to microbial metabolism, the instant detection of reproductive status.The microorganism detection system reliability is high, accuracy is good.The microorganism detection method, first emission light source device launch ultraviolet, and produce fluorescent effect with microbial culture medium；Secondly fluorescence reception device receives the fluorescence that fluorescent effect is produced, and the corresponding microbe metabolite concentration data of fluorescence is delivered into control device；Last control device receives the microbe metabolite concentration data of fluorescence reception device conveying and is analyzed, and obtains the concentration of the metabolin of microorganism.The method has precision and efficiency of detecting higher.
Description
Technical field
The present invention relates to microorganism detection field, in particular to a kind of microorganism detection system and method.
Background technology
Microorganism, including the major class biota including bacterium, virus, fungi and some small-sized protozoans etc.
Body, it is individual small, it is closely related with human lives.Relate generally to the numerous areas such as health, medicine, industrial or agricultural, environmental protection.It is general next
Say, microorganism is divided into following 8 major class：Bacterium, virus, fungi, actinomyces, Richettsia, mycoplasma, Chlamydia and spiral shell
Rotation body.For the detection of microorganism, frequently with microscope, staining technique, culture medium technology of preparing, be inoculated with, isolate and purify and
Culture technique.
And among these, culture medium technology of preparing application is more.Specifically, in the prior art, frequently with by microbial inoculant
Above to the synthetic medium for being suitable to growth and breeding, and then it is detected.But, this detection method wastes time and energy, inspection
Survey effect poor.Another some having in the prior art, growth, breeding, the generation of microorganism are detected using the method for pH color interpretations
Thank to situation, but this method sensitivity is low, low-response, error are big.Some having again provide some detections and set in the prior art
Standby or method, but sensitivity of its detection is relatively low, and and the service life of equipment is shorter, and it is high to cause equipment cost, economical
It is of poor benefits.
The content of the invention
It is an object of the invention to provide a kind of microorganism detection system, its can quickly, it is reliable, detect microorganism exactly
Breeding, metabolic condition.And long service life, financial cost is low.
Another object of the present invention is to provide a kind of microorganism detection method, its can delicately, response quickly ground, by mistake
Difference detects breeding, the metabolic condition of microorganism smallly.
To achieve these goals, the technical scheme that the embodiment of the present invention is used is as follows：
A kind of microorganism detection system, it is adaptable to detect growth, the breeding situation of microorganism, it includes：
Microbial culture medium apparatus for placing, for placing microbial culture medium.Wherein, in microbial culture medium apparatus for placing
Jogging switches measurement device is provided with, what the Reagent Tube for detecting microbial culture medium whether there is.Emission light source device, for sending out
Penetrate the ultraviolet that fluorescent effect is produced with microbial culture medium.Fluorescence reception device, the fluorescence for receiving fluorescent effect generation,
And export the corresponding microbe metabolite concentration data of fluorescence.Control device, for receiving microbe metabolite concentration data
And be analyzed.Microbial culture medium apparatus for placing, emission light source device and fluorescence reception device are electrically connected with control device
Connect.
In preferred embodiments of the present invention, it is additionally provided with microbial culture medium apparatus for placing for for microorganism gives birth to
Long, breeding provides the thermostat of preference temperature, and thermostat is connected to control device.
In preferred embodiments of the present invention, division board is provided between emission light source device and fluorescence reception device.
In preferred embodiments of the present invention, microorganism detection system is also included for passing on the instant culture of microorganism to believe
The suggestion device of breath, suggestion device is connected to control device.
In preferred embodiments of the present invention, suggestion device includes display device and sound-producing device, display device and sounding
Device is all connected to control device.
In preferred embodiments of the present invention, display device is display, and sound-producing device is buzzer.
In preferred embodiments of the present invention, control device is control mainboard.
A kind of microorganism detection method, is detected, microorganism detection method bag using above-mentioned microorganism detection system
Include：
Emission light source device launches ultraviolet, and produces fluorescent effect with microbial culture medium；Fluorescence reception device is received
The fluorescence that fluorescent effect is produced, and the corresponding microbe metabolite concentration data of fluorescence is delivered to control device；Control device
The microbe metabolite concentration data for receiving the conveying of fluorescence reception device is simultaneously analyzed, obtain microorganism metabolin it is dense
Degree.
In preferred embodiments of the present invention, before emission light source device transmitting ultraviolet, filled using jogging switches measurement
Put and the information conveyance of Reagent Tube to control device will be whether there is in microbial culture medium apparatus for placing.
In preferred embodiments of the present invention, in detection process, microbial culture medium is placed using thermostat is filled
Temperature information in putting is delivered to control device, and the temperature stated in microbial culture medium apparatus for placing is carried out by control device
Control.
The beneficial effects of the invention are as follows：
The present invention provide microorganism detection system, by set microbial culture medium apparatus for placing, emission light source device,
Fluorescence reception device and control device, in control device and emission light source device, fluorescence reception device and microculture
Under the collaborative work of liquid apparatus for placing, realize to microbial metabolism, the instant detection of reproductive status.The microorganism detection system
Reliability is high, accuracy is good.
The microorganism detection method that the present invention is provided, first emission light source device transmitting ultraviolet, and and microculture
Liquid produces fluorescent effect；Secondly fluorescence reception device receives the fluorescence that fluorescent effect is produced, and by fluorescence corresponding microorganism generation
Thank to thing concentration data and be delivered to control device；Last control device receives the microbe metabolite concentration of fluorescence reception device conveying
Data are simultaneously analyzed, and obtain the concentration of the metabolin of microorganism.The method has precision and efficiency of detecting higher.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, below will be attached to what is used needed for embodiment
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, thus be not construed as it is right
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is the decomposition texture schematic diagram of microorganism detection system provided in an embodiment of the present invention；
Fig. 2 is the structural representation at the first visual angle of microorganism detection system provided in an embodiment of the present invention；
Fig. 3 is microbial culture medium apparatus for placing and the control master of microorganism detection system provided in an embodiment of the present invention
The structural representation of plate；
Fig. 4 is the structural representation at the second visual angle of microorganism detection system provided in an embodiment of the present invention；
Fig. 5 is the structural representation of emission light source device provided in an embodiment of the present invention and fluorescence reception device；
Fig. 6 shows the workflow diagram of the microorganism detection system that the embodiment of the present invention is provided.
Icon：100- microorganism detection systems；110- housings；111- upper casings；112- lower casings；113- printing opacity posts；114- prevents
Dust hood；115- connecting poles；116- connecting holes；117- connection lids；118- working ends；120- microbial culture medium apparatus for placing；
121- thermostats；122- jogging switches measurement devices；131- emission light source devices；132- fluorescence reception devices；133- is ultraviolet
Bulb；134- photoelectric sensors；135- division boards；140- control devices；141- control mainboards；150- Reagent Tubes；161- shows
Device；162- sound-producing devices；171- serial ports；172-RJ45 interfaces；173-USB interfaces；174- power interfaces.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
A part of embodiment of the present invention, rather than whole embodiments.Present invention implementation generally described and illustrated in accompanying drawing herein
The component of example can be arranged and designed with a variety of configurations.
Therefore, the detailed description of embodiments of the invention below to providing in the accompanying drawings is not intended to limit claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
It should be noted that：Similar label and letter represents similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined in individual accompanying drawing, then it need not be further defined and explained in subsequent accompanying drawing.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", D score, "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outward " be based on orientation shown in the drawings or position relationship, or should
Orientation or position relationship that invention product is usually put when using, are for only for ease of the description present invention and simplify description, without
Be indicate or imply meaning device or element must have specific orientation, with specific azimuth configuration and operation, therefore not
It is understood that to be limitation of the present invention.Additionally, term " first ", " second ", " the 3rd " etc. are only used for distinguishing description, and can not manage
Solve to indicate or implying relative importance.
Additionally, the term such as term " level ", " vertical ", " pendency " is not offered as requiring part abswolute level or pendency, and
Can be to be slightly tilted.It is not the expression structure if " level " refers to only its direction with respect to more level for " vertical "
Must be fully horizontal, and can be to be slightly tilted.
In the description of the invention, in addition it is also necessary to explanation, unless otherwise clearly defined and limited, term " setting ",
" installation ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or one
The connection of body ground；Can mechanically connect, or electrically connect；Can be joined directly together, it is also possible to indirect by intermediary
It is connected, can is two connections of element internal.For the ordinary skill in the art, can be with concrete condition understanding
State term concrete meaning in the present invention.
Incorporated by reference to Fig. 1, Fig. 2 and Fig. 3, the present embodiment provides a kind of microorganism detection system 100, and it includes, microorganism
Nutrient solution apparatus for placing 120, for placing microbial culture medium；Fine motion is provided with microbial culture medium apparatus for placing 120 to open
Closing detecting apparatus 122, the Reagent Tube 150 of microbial culture medium is whether there is for detecting in microbial culture medium apparatus for placing 120；Hair
Light supply apparatus 131 is penetrated, the ultraviolet of fluorescent effect is produced with microbial culture medium for launching；Fluorescence reception device 132, is used for
The fluorescence that fluorescent effect is produced is received, and the corresponding microbe metabolite concentration data of fluorescence is exported；Control device 140, uses
Microbe metabolite concentration data and it is analyzed in receiving.Wherein, microbial culture medium apparatus for placing 120, transmitting light source dress
Put 131 and fluorescence reception device 132 electrically connected with control device 140.
Above-mentioned microorganism detection system 100 also includes housing 110.Above-mentioned microbial culture medium apparatus for placing 120, with
And control device 140 may be contained within housing 110.Further, housing 110 includes upper casing 111 and lower casing 112, and upper casing 111 is covered
Together in lower casing 112.Specifically, connecting pole 115 is provided with each drift angle of the lower surface of upper casing 111, correspondence is in lower casing 112
Each drift angle at be provided with connecting hole 116 such that it is able to cooperated with connecting pole 115 so that upper casing 111 insertion its
In, so as to be covered on lower casing 112.This connected mode is not only reliable, and more saves space, causes whole microorganism
The overall structure of detecting system 100 is compacter.
Further, connection lid 117 is additionally provided with the outer surface of connecting pole 115, the one end for connecting lid 117 is connected to
Shell 111, the other end is connected to lower casing 112 such that it is able to further so that the connection of upper casing 111 and lower casing 112 is more
It is reliable, and enable to the junction of upper casing 111 and lower casing 112 more with sealing, further protection setting is in shell
Other devices inside body 110, and then ensure that the reliability of operation of microorganism detection system 100.
It should be noted that the shape of above-mentioned housing 110 is not limited.Above-mentioned housing 110 can with shape can be with
Select shape, cylinder or other columns and others of the casing of cuboid or square according to the actual needs
The irregular shape being processed into.Preferably in the present embodiment, shape selection cuboid or the square of above-mentioned housing 110
Box shape.
Incorporated by reference to Fig. 1 and Fig. 2, microbial culture medium apparatus for placing 120 is arranged in above-mentioned housing 110, and micro- life
Expose the upper surface of housing 110 in the upper surface of thing nutrient solution apparatus for placing 120.So as to more facilitate the reagent of microbial culture medium
Pipe 150 is positioned in microbial culture medium apparatus for placing 120, and then for follow-up operating procedure provides sound assurance.It is preferred that
Ground, microbial culture medium apparatus for placing 120 is exposed to the working end 118 in one end of the upper surface of housing 110, i.e. reference picture 2.
So as to more facilitate operating personnel to be used and be operated microorganism detection system 100.
Further, the shape of microbial culture medium apparatus for placing 120 is not limited.Preferably, in microculture
The upper surface of liquid apparatus for placing 120 is provided with multiple holes for placing Reagent Tube 150 such that it is able to by microbial culture medium
Reagent Tube 150 is positioned over the upper surface of microbial culture medium apparatus for placing 120 and sets in the hole for opening up.
Further, it is additionally provided with as growth of microorganism provides preference temperature inside microbial culture medium apparatus for placing 120
Thermostat 121, thermostat 121 is connected to control device 140.So as to according to different types of microorganism or not
With the culture demand in stage, the pre-set temperature in control device 140, and then realize to the control of thermostat 121 and
Regulation.It should be noted that the set location of thermostat 121 is not limit ground.Preferably, thermostat 121 is arranged at micro-
On the inwall of bio-culture solution apparatus for placing 120.Further, the mode of the regulation temperature of thermostat 121 is also not limit.
Specifically, the mode of the regulation temperature of thermostat 121 can select temperature controller temperature adjustment, resistance wire temperature adjustment or other bases
It is actually needed other method of temperature control of design.In the present embodiment, microorganism detection system 100 is the base in bionidicator
Nutrient solution on plinth using bacillus stearothermophilus/hay bacillus metabolin and special component in growth, reproductive process is produced instead
Should, reaction product can produce fluorescent effect under ultraviolet irradiation, and fluoroscopic examination is carried out using photosensitive reception sensor, according to
The strong and weak concentration for realizing quickly judging specific metabolite of fluorescence for detecting, so as to learn the survival condition of gemma.Therefore, for
The culture of different microorganisms needs the different temperature value of setting, so as to corresponding microorganism could be enabled in suitable temperature
Grown under degree.And pass through to set thermostat 121, can effectively ensure that microorganism is normally cultivated, so as to be follow-up
Operation necessary guarantee is provided.
Further, incorporated by reference to Fig. 1 and Fig. 3, it is additionally provided with inside microbial culture medium apparatus for placing 120 for detecting examination
The jogging switches measurement device 122 that agent pipe 150 whether there is.Microswitch is with small contact separation and snap-action mechanism, with regulation
Stroke and the power of regulation carry out the contact mechanism of switch motion, covered with shell, have a kind of switch of drive rod outside it, because
For the contact spacing that it is switched is smaller, therefore named microswitch is called sensitive switch.Outer mechanical force passes through actuated element by masterpiece
For acting reed, snap action is produced when acting reed and being displaced to critical point, make the movable contact of action reed end with
Fixed contact quick-make or disconnection.After the active force on actuated element is removed, action reed produces counteragent power, works as transmission
After element reverse stroke reaches the action critical point of reed, counteragent is instantaneously completed.The contact spacing of microswitch is small, action
Stroke is short, by power is small, break-make is rapid.The responsiveness of its movable contact is unrelated with actuated element responsiveness.Relative to existing
Optoelectronic switch in technology, microswitch can more delicately detect inside microbial culture medium apparatus for placing 120 whether
It is placed with Reagent Tube 150.So as to preferably avoid not placing the microbial culture medium apparatus for placing 120 of Reagent Tube 150
Hole in be also carried out correlation heating or light irradiation etc. operation.And then the luminous tube in can effectively protecting, so as to have
The service life of effect ground extension luminous tube, and then ensure that reliability, accuracy that microorganism detection system 100 is used.Also make
The service life for obtaining microorganism detection system 100 increases, and reduces financial cost.
Fig. 1 is refer to, further, the working end 118 of microbial culture medium apparatus for placing 120 is additionally provided with printing opacity post
113 and dust cover 114.Specifically, printing opacity post 113 and dust cover 114 are arranged on upper casing 111.It should be noted that thoroughly
The number of light beam 113 is multiple, specifically, is connected at side with each of lower casing 112 in upper casing 111, is provided with printing opacity post
113.The setting of printing opacity post 113 can effectively ensure in transmitting light source be irradiated to microbial culture medium through printing opacity post 113
Apparatus for placing 120 is built with the Reagent Tube 150 of microbial culture medium.Now, the metabolin of microorganism and spy in Reagent Tube 150
The nutrient solution for determining composition produces reaction, and reaction product can be produced fluorescent effect, be passed using photosensitive reception under ultraviolet irradiation
Sensor carries out fluoroscopic examination, according to the strong and weak concentration for realizing quickly judging specific metabolite of the fluorescence for detecting, so as to learn bud
The survival condition of spore.In addition, the setting of dust cover 114 can effectively ensure microbial culture medium apparatus for placing 120 by outer
The damage of other pollutants in the dust on boundary, dust and air to microbial culture medium apparatus for placing 120, so as to ensure
The accuracy and reliability of the operation of microbial culture medium apparatus for placing 120, and then ensure that the standard of microorganism detection system 100
True property and reliability, extend the service life of microorganism detection system 100.
Incorporated by reference to Fig. 1 and Fig. 3, further, the rear portion of microbial culture medium apparatus for placing 120 is arranged at.Specifically, including
Emission light source device 131 and fluorescence reception device 132, emission light source device 131 and fluorescence reception device 132 are all connected to control
Device 140.
Specifically, emission light source device 131 includes multiple UV lamp bulbs or ultraviolet lamp tube.Preferably, in this implementation
In example, emission light source device 131 selects ultraviolet bulb 133, can more easily be installed in emission light source device 131, and
Coordinate with microbial culture medium apparatus for placing 120.So as to the ultraviolet lighting sent when ultraviolet bulb 133 is mapped to microbial culture medium
Reagent in apparatus for placing 120, and with Reagent Tube 150 in microbial culture medium in fluorescent material occur fluorescence reaction, from
And the concentration data of the corresponding microbe metabolite of fluorescence is delivered to control device 140, and then 140 pairs of feedbacks of control device
Data are analyzed treatment, obtain metabolism, the relevant information of breeding of microorganism.
Specifically, fluorescence reception device 132 can select photoelectric sensor 134, it is also possible to selecting other types can
Receive and transmit the element of fluorescence signal.Preferably, in the present embodiment, the selection of fluorescence reception device 132 photoelectric sensor
134 such that it is able to which the fluorescence signal that will be received is converted into electric signal, and then is transported to control device 140, and signal is entered
Row treatment.Finally give metabolism, the relevant information of breeding of microorganism.Photoelectric sensor 134 converts optical signals to electric signal
Structural principle be well-known to those skilled in the art, here is omitted.
Further, Fig. 4 is refer to, division board is provided between emission light source device 131 and fluorescence reception device 132
135.Ultraviolet light so as to be effectively prevented from the transmitting of emission light source device 131 is directly irradiated on fluorescence reception device 132.
The ultraviolet light that emission light source device 131 is launched only first is irradiated in the fluorescence in the microbial culture medium in Reagent Tube 150
Matter, after there is fluorescence reaction with it, then reflects or reflects on fluorescence reception device 132, could realizing Reagent Tube 150
In microorganism metabolism, the information of breeding by optical signal feedback to fluorescence reception device 132, and then it is defeated to change into electric signal
Deliver to control device 140.
Fig. 3 is refer to, control device 140 includes control mainboard 141 and for processing and preserving data, review sterilizing letter
The software systems that breath, Chinese and English interface are switched fast.Relative program and threshold value are pre-set in software systems, can be to anti-
The electric signal for being fed to software systems is processed, and obtains testing result, and control whole microorganism detection system 100 normally to transport
OK.Specifically, microbial culture medium apparatus for placing 120, be all connected to control device 140.Specifically, microbial culture medium is placed
Thermostat 121 in device 120 is connected to control device 140, in detection process, so that will be micro- using thermostat 121
Temperature information in bio-culture solution apparatus for placing 120 is delivered to control device 140, and microorganism is trained by control device 140
Temperature in nutrient solution apparatus for placing 120 is controlled.Jogging switches measurement device in microbial culture medium apparatus for placing 120
122 are connected to control device 140, so as to before the transmitting ultraviolet of emission light source device 131, using jogging switches measurement device
122 will whether there is the information conveyance of Reagent Tube 150 to control device 140 in microbial culture medium apparatus for placing 120.In launching light
Source device 131 and fluorescence reception device 132 are connected with control device 140.Specifically, emission light source device 131 launches ultraviolet
Line, and produce fluorescent effect with microbial culture medium；Fluorescence reception device 132 receives the fluorescence that fluorescent effect is produced, and will be glimmering
The data of the metabolite concentration of the corresponding microorganism of light are delivered to control device 140.Connect so as to control device 140 receives fluorescence
The data of the metabolite concentration of the corresponding microorganism of fluorescence of the conveying of receiving apparatus 132, to data and analyze, and then obtain micro-
The concentration of biological metabolin.
Further, Fig. 1 and Fig. 3 is refer to, microorganism detection system 100 also includes the instant training for passing on microorganism
The prompting of the information of supporting, suggestion device is connected to control device 140.Suggestion device includes display device 161 and sound-producing device 162,
Display device 161 and sound-producing device 162 are all connected to control device 140.Specifically, in this example it is shown that device 161 is
Display, sound-producing device 162 is buzzer.Display preferably, information exchange is carried out using touch-screen, so that microorganism
Detecting system 100 is more user-friendly.In addition, when equipment breaks down, prompting letter can also occur in display device 161
Breath, points out user to use the mistake or other information of operation.Meanwhile, the also synchronous sound that sounds the alarm of sound-producing device 162 is pointed out
User's operating mistake or other information.Pointed out by this sound and light alarm, allow operator more fully to understand culture letter immediately
Breath.Further, the software systems in above-mentioned control device 140 are provided with the function that Chinese and English interface is switched fast, and enter one
Step causes microorganism detection system 100, and functional diversities more are user-friendly to.
Fig. 5 is refer to, microorganism detection system 100 also includes data outputting unit, and data outputting unit is in control device
140 connections.Specifically, the data outputting unit includes serial ports 171, RJ45 interfaces 172, usb 1 73 and power interface
174.Specifically, data can be exported by connecting printer, it is also possible to by wireless blue tooth, to transferring data to
On mobile phone or flat board, or connection computer receiving data.It is achieved thereby that will detect in microorganism detection system 100
Microbial metabolism, the instant messages of breeding are transferred out.
Generally speaking, the operation principle of above-mentioned microorganism detection system 100 is：Microorganism detection system 100 is in life
On the basis of thing indicant using bacillus stearothermophilus/hay bacillus in growth, reproductive process metabolin and special component
Nutrient solution produces reaction, and reaction product can produce fluorescent effect under ultraviolet irradiation, be carried out using photosensitive reception sensor
Fluoroscopic examination, according to the strong and weak concentration for realizing quickly judging specific metabolite of the fluorescence for detecting, so as to learn the survival of gemma
Situation.The assembling of above-mentioned microorganism detection system 100 is：Control mainboard 141 is installed in lower casing 112 first, secondly will
Microbial culture medium apparatus for placing 120 and it is installed on lower casing 112 and is connected on control device 140.Then upper casing 111 is covered
In on lower casing 112.Dust cover 114 and printing opacity post 113 are installed on housing 110, display device 161 is installed on housing
On 110, and control device 140 is connected to, that is, completes the assembling of microorganism detection system 100.
Fig. 6 is refer to, embodiments of the invention additionally provide a kind of microorganism detection method, examined using above-mentioned microorganism
Examining system 100 is detected, comprised the following steps：
First, the transmitting of emission light source device 131 ultraviolet, and produce fluorescent effect with microbial culture medium.Specifically, open
Dynamic microorganism detection system 100, before the transmitting ultraviolet of emission light source device 131, will be micro- using jogging switches measurement device 122
The information conveyance of Reagent Tube 150 to control device 140 is whether there is in bio-culture solution apparatus for placing 120.In addition in detection process,
The temperature information in microbial culture medium apparatus for placing 120 is delivered to control device 140 using thermostat 121, by control
140 pairs of temperature stated in microbial culture medium apparatus for placing 120 of device processed are controlled.
Secondly, fluorescence reception device 132 receives the fluorescence that fluorescent effect is produced, and by the corresponding microbe metabolite of fluorescence
Concentration data is delivered to control device 140；
Finally, control device 140 receives the microbe metabolite concentration data of the conveying of fluorescence reception device 132 and is divided
Analysis, obtains the concentration of the metabolin of microorganism.Specifically, it is the metabolism of the metabolite concentration or microorganism of the microorganism for obtaining, numerous
Situation is grown, can be reported by display or buzzer.
In sum, the microorganism detection system that embodiments of the invention are provided, is placed by setting microbial culture medium
Device, emission light source device, fluorescence reception device and control device, in control device and emission light source device, fluorescence reception
Under the collaborative work of device and microbial culture medium apparatus for placing, realize to microbial metabolism, the instant inspection of reproductive status
Survey.The microorganism detection system reliability is high, accuracy is good.
Embodiments of the invention provide microorganism detection method, first emission light source device transmitting ultraviolet, and with it is micro-
Bio-culture solution produces fluorescent effect；Secondly fluorescence reception device receives the fluorescence that fluorescent effect is produced, and fluorescence is corresponding
Microbe metabolite concentration data is delivered to control device；Last control device receives the microorganism generation of fluorescence reception device conveying
Thank to thing concentration data and be analyzed, obtain the concentration of the metabolin of microorganism.The method has accuracy of detection and effect higher
Rate.
The preferred embodiments of the present invention are the foregoing is only, is not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.It is all within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.It should be noted that：Similar label and letter exists
Similar terms is represented in following accompanying drawing, therefore, once being defined in a certain Xiang Yi accompanying drawing, then it is not required in subsequent accompanying drawing
It is further defined and is explained.
Claims (10)
1. a kind of microorganism detection system, it is adaptable to detect growth, the breeding situation of the microorganism, it is characterised in that its bag
Include：
Microbial culture medium apparatus for placing, for placing microbial culture medium；Set in the microbial culture medium apparatus for placing
There is jogging switches measurement device, whether there is the examination of the microbial culture medium in the microbial culture medium apparatus for placing for detecting
Agent pipe；
Emission light source device, the ultraviolet of fluorescent effect is produced for launching with the microbial culture medium；
Fluorescence reception device, for receiving the fluorescence that the fluorescent effect is produced, and by the corresponding microbial metabolism of the fluorescence
Thing concentration data is exported；
Control device, for receiving the microbe metabolite concentration data and being analyzed, and the jogging switches measurement is filled
Put, the emission light source device and the fluorescence reception device are electrically connected with the control device.
2. microorganism detection system as claimed in claim 1, it is characterised in that in the microbial culture medium apparatus for placing also
Thermostat is provided with, preference temperature is provided for the growth for the microorganism, breeding, the thermostat is connected to described
Control device.
3. microorganism detection system as claimed in claim 1, it is characterised in that the emission light source device and the fluorescence connect
Division board is provided between receiving apparatus.
4. microorganism detection system as claimed in claim 2 or claim 3, it is characterised in that the microorganism detection system also includes
Suggestion device for passing on the instant culture information of the microorganism, the suggestion device is connected to the control device.
5. microorganism detection system as claimed in claim 4, it is characterised in that the suggestion device includes display device and hair
Acoustic device, the display device and the sound-producing device are all connected to the control device.
6. microorganism detection system as claimed in claim 5, it is characterised in that the display device is display, the hair
Acoustic device is buzzer.
7. microorganism detection system as claimed in claim 1, it is characterised in that the control device is control mainboard.
8. a kind of microorganism detection method, it is characterised in that using the microorganism detection system as described in claim any one of 1-7
System is detected that the microorganism detection method includes：
The emission light source device launches ultraviolet, and produces fluorescent effect with the microbial culture medium；
The fluorescence reception device receives the fluorescence that the fluorescent effect is produced, and by the corresponding micro- life of the fluorescence
Thing metabolite concentration data are delivered to the control device；
The control device receives the microbe metabolite concentration data of the fluorescence reception device conveying and is analyzed,
Obtain the concentration of the microbe metabolite.
9. microorganism detection method as claimed in claim 8, it is characterised in that launch ultraviolet in the emission light source device
Before, using the jogging switches measurement device by the information conveyance for whetheing there is Reagent Tube in the microbial culture medium apparatus for placing extremely
The control device.
10. microorganism detection method as claimed in claim 8, it is characterised in that in detection process, will using thermostat
Temperature information in the microbial culture medium apparatus for placing is delivered to the control device, by the control device to described
Temperature in microbial culture medium apparatus for placing is controlled.
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