WO2019084969A1

WO2019084969A1 - Immersion biomass on-line detection apparatus

Info

Publication number: WO2019084969A1
Application number: PCT/CN2017/109580
Authority: WO
Inventors: 傅雄飞; 万玉明; 艾澈熙; 王�忠; 崔金明; 刘陈立; 袁海
Original assignee: 广州中国科学院先进技术研究所
Priority date: 2017-11-06
Filing date: 2017-11-06
Publication date: 2019-05-09
Also published as: CN108064338A

Abstract

An immersion biomass on-line detection apparatus, comprising a light source (2), an optical signal receiver (3), a control circuit board (4) and a waterproof shell (1), wherein the light source (2), the optical signal receiver (3) and the control circuit board (4) are all packaged in the waterproof shell (1); the control circuit board (4) is respectively and electrically connected to the light source (2) and the optical signal receiver (3); the waterproof shell (1) realizes the protection of internal elements; a certain optical path is formed between the light source (2) and the optical signal receiver (3), and the optical path passes through a tested sample; the light source (2) and the optical signal receiver (3) pass through two windows on the waterproof shell (1) to detect the biomass of the tested sample by means of detecting an optical density value; and the control circuit board (4) receives an external control signal to implement a relevant control function, and sends the detected biomass to external personnel for viewing, so that online detection of microbial growth is realized. The scheme can realize on-line detection of biomass, thereby avoiding the tedious operation brought out by manual sampling detection of an offline measurement method and the risk of easy infection of bacteria. Moreover, the scheme is applicable to a petri dish or a fermentation tank of any shape.

Description

Immersion biomass online detecting device

Technical field

The invention relates to the technical field of biological experimental equipment, in particular to an immersion biomass online detecting device.

Background technique

In bioengineering or fermentation engineering, a certain amount of microorganisms are often inoculated in a liquid medium and then stored in a specific environment. In order to monitor the growth state of microorganisms in the medium, it is usually necessary to perform detection by methods such as optical density (OD) measurement.

At present, the most common optical density (OD) measurement method in the laboratory is that a part of the sample is manually taken out by the scientific researcher and taken offline by an optical density (OD) detector.

CN105044038A discloses a non-invasive biomass on-line detection device for shake flask culture, the device comprising a main control unit, a shake flask for holding a biological suspension, and a fixed sleeve covering the outside of the shake flask. The device enables on-line detection of the concentration of the biological suspension in the shake flask.

At present, the most common offline optical density (OD) measurement method in the laboratory requires manual sampling by researchers, which is cumbersome and easy to infect bacteria.

CN105044038A discloses a non-invasive biomass on-line detecting device for shake flask culture. Since there is a special cover sleeve on the outer side of the shake flask, one device is only suitable for a shake flask of a specific shape and has poor versatility.

Summary of the invention

In view of this, in order to solve the above problems in the prior art, the present invention aims to propose an immersion biomass online detecting device. The device can realize on-line detection of biomass, avoiding the cumbersome operation caused by manual sampling and detection of off-line measurement methods and the risk of being susceptible to infection; and the device is suitable for any In-line detection of internal biomass can be achieved by simply placing the device in a petri dish or fermentor.

The present invention solves the above problems by the following technical means:

In one aspect, the present invention provides an in-line biomass on-line detecting device, including a light source, an optical signal receiver, a control circuit board, and a waterproof casing; the light source, the optical signal receiver, and the control circuit board are all packaged in a waterproof casing, and the control circuit The board is electrically connected to the light source and the optical signal receiver respectively, and the waterproof casing realizes protection of internal components, and a certain optical path is formed between the light source and the optical signal receiver, the optical path passes through the sample to be tested, and the light source and the optical signal receiver pass through the waterproof casing. The upper two windows detect the biomass of the sample to be tested by detecting the optical density value, the control circuit board receives the external control signal to realize the relevant control function, and sends the measured biomass to an external person for viewing, thereby realizing the microbial growth online. Detection.

Further, the immersion biomass on-line detecting device is powered by an internal battery or by a wired external power supply; when the internal battery is used for power supply, the internal battery is also included, the internal battery is packaged in a waterproof casing, and the internal battery is integrated in the control circuit. The board is electrically connected to the control board.

Further, the immersed biomass online detecting device adopts a wireless signal transmission mode or a wired signal transmission mode; when the wireless signal transmission mode is adopted, the wireless signal transmission unit is further included, and the wireless signal transmission unit is packaged in a waterproof casing, and wirelessly The signal transmission unit is integrated on the control circuit board and electrically connected to the control circuit board.

Further, the wireless signal transmission unit is a Wifi module, an RFID module or a Bluetooth module.

Further, the light path of the light source and the optical signal receiver is directly transmissive or reflective.

Further, the waterproof outer casing is an ellipsoidal shape, a cross shape or a cylindrical shape.

Further, the waterproof casing is waterproofed by a sealing ring, a sealant, a thread seal or an interference fit.

Further, the light source uses an LED and a laser diode, and the optical signal receiver uses a photodiode or a phototransistor.

Further, the immersion biomass on-line detecting device further comprises a stirring rod, and the stirring rod is a kind The magnetic rod with the N pole and the S pole is enclosed in a waterproof casing, and the external rotating magnetic field drives the rotation to drive the whole device to rotate, thereby achieving the purpose of stirring.

In another aspect, the present invention also provides an on-line biomass on-line detecting device, comprising a light source, an optical signal receiver, and a control circuit board; the light source, the optical signal receiver and the control circuit board are all waterproofed, and the control circuit board respectively The light source and the optical signal receiver are electrically connected, and a certain optical path is formed between the light source and the optical signal receiver, and the optical path passes through the sample to be tested, and the light source and the optical signal receiver detect the biomass of the sample to be tested by detecting the optical density value, and the control circuit board receives The external control signal implements the relevant control functions and sends the measured biomass to an external person for viewing, thereby enabling on-line detection of microbial growth.

Compared with the prior art, the beneficial effects of the present invention are as follows:

1) Realizing on-line detection of biomass in the culture solution, avoiding the cumbersome operation of manual sampling and the risk of infecting bacteria during sampling;

2) Applicable to any shape of culture dish or fermenter, the on-line detection of biomass in the culture solution can be realized by simply placing the device in a culture dish or a fermenter;

3) All components can be packaged in a waterproof casing, which is compact and easy to use;

4), can be combined with other commonly used tools for biological experiments, and has strong versatility.

DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present invention. Other drawings may also be obtained from those of ordinary skill in the art in light of the inventive work.

1 is a schematic view showing a usage scenario of Embodiment 1 of the immersion biomass on-line detecting device of the present invention;

2 is a schematic diagram of a wireless structure of Embodiment 1 of the immersion biomass on-line detecting device of the present invention (where A is a full view of the main view and B is an exploded view);

3 is a schematic view showing a wired structure of Embodiment 1 of the immersion biomass on-line detecting device of the present invention (where A is a full view of the main view and B is an exploded view);

4 is a schematic diagram of a combination design of the embodiment 1 of the immersion biomass on-line detecting device of the present invention and a stirring rod (where A is a full view of the main view and B is an exploded view);

5 is a schematic diagram of several specific optical paths of Embodiment 1 of the immersion biomass on-line detecting device of the present invention;

6 is a schematic view showing the structure of several specific shapes of the embodiment 1 of the immersion biomass on-line detecting device of the present invention;

Description of the reference signs:

1, waterproof housing, 2, light source, 3, optical signal receiver, 4, control circuit board, 5, stir bar, 10, petri dish, 20, culture solution, 30, immersion biomass online detection device.

Detailed ways

The above described objects, features, and advantages of the present invention will be more apparent from the aspects of the appended claims. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments, based on the embodiments of the present invention, all of which are obtained by those skilled in the art without creative efforts. Other embodiments are within the scope of the invention.

Example 1

The invention aims to propose an immersion biomass on-line detecting device, which does not require manual sampling and detection, so that scientific researchers can be freed from frequent manual operations, and the risk of artificially operating infected bacteria is greatly reduced. Its use scene is shown in Figure 1, (in the figure: 10, culture dish, 20, culture solution, 30, immersion biomass online detection device).

The immersion biomass on-line detecting device 30 of the present invention mainly comprises a light source 2, an optical signal receiver 3, a control circuit board 4, and a waterproof casing 1. The light source 2, the optical signal receiver 3, and the control circuit board 4 are all housed in the waterproof case 1, and the control circuit board 4 is electrically connected to the light source 2 and the optical signal receiver 3, respectively.

The light source 2 and the optical signal receiver 3 realize measurement of the sample to be tested, the control circuit board 4 realizes relevant control functions, and the waterproof casing 1 realizes protection of internal components.

A certain optical path is formed between the light source 2 and the optical signal receiver 3, and the optical path must pass through the sample to be tested.

The light source 2 and the optical signal receiver 3 detect the sample to be tested through two windows on the waterproof casing 1. It is set to automatically detect at regular intervals to achieve on-line detection of microbial growth.

The unit can be powered by an internal battery or by a wired external power supply.

The device can realize the "receiving/sending" of external control signals and measured data, and can adopt wireless signal transmission methods (Wifi, RFID, Bluetooth, etc.) or wired signal transmission.

The waterproof casing 1 mainly protects the internal components, and the waterproof casing 1 is formed into a simple shape, which can also reduce the adhesion of microorganisms on the device; if the internal components themselves are waterproofed, the waterproof casing 1 can also be eliminated.

The light source 2 may be an LED, a laser diode or the like, and the optical signal receiver 3 may be a photodiode or a phototransistor.

Several possible specific solutions

(A) wireless design

The components such as the power source (internal battery) and the signal "receiving/transmitting" (wireless signal transmission unit) are all integrated on the control circuit board 4, installed inside the waterproof casing 1, and the light source 2 and the optical signal receiver 3 are transmitted through the waterproof casing. The two windows on 1 detect the sample being tested. The relevant structure is shown in Figure 2.

(B) Wired design

It is connected to the internal control circuit board 4 by means of a wired external power supply or a wired signal transmission method, and the other parts are similar to the scheme A. The relevant structure is shown in Figure 3.

(C) combined with other commonly used tools

Various tools are often required during microbial culture. For example, when the microorganism is cultured, it is often necessary to continuously stir the culture solution with the stirring rod 5 to promote the growth of the microorganisms. Therefore, the apparatus can be designed to be combined with the stirring rod 5 to measure the biomass in the culture liquid while stirring. of purpose. The related structure is as shown in FIG. 4, and the stirring rod 5 penetrates the control circuit board 4 and is enclosed in the waterproof case 1. "Penetration" is one of its arrangements, in addition to this, there may be other arrangements, as long as the stir bar 5 can drive the entire device to rotate normally.

The stirring rod 5 is a magnetic ceramic rod with N poles and S poles, and has a size of about Φ 15 × 60 mm, and can be rotated by an externally rotating magnetic field, thereby achieving non-contact rapid stirring. The invention combines the stirring rod 5 with the detecting device. During the stirring process, the stirring rod 5 drives the whole device to rotate, thereby achieving the purpose of measuring while stirring.

The invention has the following characteristics:

1) When using a wired design, the power supply can be both external and internal. When using a wireless design, the power supply mode can be internal battery installation or electromagnetic induction effect;

2) The optical path of the light source 2 and the optical signal receiver 3 can be either direct transfer or reflective. The optical path can be realized by means of optical fiber guiding, specular reflection, and the like. Several specific optical path propagation modes are shown in Figure 5: (In the figure, L is the light source 2, S light signal receiver 3)

3) The outer shape of the waterproof casing 1 can be various, such as an ellipsoidal shape, a cross shape, a cylindrical shape, and the like. As shown in Figure 6.

4), waterproof housing 1 can use sealing ring, sealant, thread seal, interference fit and other specific waterproof methods;

5) If the internal components themselves are waterproofed, the waterproof case 1 can also be eliminated.

Example 2

The invention also provides an on-line biomass on-line detecting device, comprising a light source, an optical signal receiver and a control circuit board; the light source, the optical signal receiver and the control circuit board are all waterproofed, and the control circuit board is respectively connected with the light source and the optical signal The receiver is electrically connected, a certain optical path is formed between the light source and the optical signal receiver, and the optical path passes through the sample to be tested. The light source and the optical signal receiver detect the biomass of the sample to be tested by detecting the optical density value, and the control circuit board receives the external control signal. Relevant control functions, and the measured biomass is sent to external personnel for viewing, thereby enabling online detection of microbial growth.

The above-mentioned embodiments are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but is not to be construed as limiting the scope of the invention. It should be noted that a number of variations and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention. Therefore, the scope of the invention should be determined by the appended claims.

Claims

An immersed biomass online detecting device, comprising: a light source, an optical signal receiver, a control circuit board and a waterproof casing; the light source, the optical signal receiver and the control circuit board are all packaged in the waterproof casing, and the control circuit board respectively Electrically connected to the light source and the optical signal receiver, the waterproof casing realizes protection of internal components, and a certain optical path is formed between the light source and the optical signal receiver, the optical path passes through the sample to be tested, and the light source and the optical signal receiver pass through the waterproof casing. The two windows detect the biomass of the sample to be tested by detecting the optical density value, and the control circuit board receives the external control signal to implement the relevant control function, and sends the measured biomass to an external person for viewing, thereby realizing online detection of microbial growth.
The immersion biomass on-line detecting device according to claim 1, wherein the immersed biomass on-line detecting device is powered by an internal battery or by a wired external power source; and when an internal battery is used for powering, the internal detecting device further includes an internal The battery, the internal battery is packaged in a waterproof case, and the internal battery is integrated on the control circuit board and electrically connected to the control circuit board.
The immersed biomass on-line detecting device according to claim 1, wherein the immersed biomass online detecting device adopts a wireless signal transmission mode or a wired signal transmission mode; when the wireless signal transmission mode is adopted, the method further includes The wireless signal transmission unit is packaged in a waterproof casing, and the wireless signal transmission unit is integrated on the control circuit board and electrically connected to the control circuit board.
The immersed biomass online detecting device according to claim 3, wherein the wireless signal transmission unit is a Wifi module, an RFID module or a Bluetooth module.
The immersion biomass on-line detecting device according to claim 1, wherein the light path of the light source and the optical signal receiver is directly transmissive or reflective.
The immersion biomass on-line detecting device according to claim 1, wherein the waterproof casing is an ellipsoidal shape, a cross shape or a cylindrical shape.
The immersion biomass on-line detecting device according to claim 1, wherein said The waterproof case is waterproof with a sealing ring, sealant, thread seal or interference fit.
The immersion biomass on-line detecting device according to claim 1, wherein the light source uses an LED and a laser diode, and the optical signal receiver uses a photodiode or a phototransistor.
The immersion biomass on-line detecting device according to claim 1, wherein the immersed biomass on-line detecting device further comprises a stirring rod, and the stirring rod is a magnetic rod with N pole and S pole, stirring The rod is packaged in a waterproof casing, and the external rotating magnetic field drives the rotation to drive the entire device to rotate, thereby achieving the purpose of stirring.
The utility model relates to a immersion biomass online detecting device, which comprises a light source, an optical signal receiver and a control circuit board; the light source, the optical signal receiver and the control circuit board are all waterproofed, and the control circuit board and the light source and the optical signal respectively The receiver is electrically connected, a certain optical path is formed between the light source and the optical signal receiver, and the optical path passes through the sample to be tested. The light source and the optical signal receiver detect the biomass of the sample to be tested by detecting the optical density value, and the control circuit board receives the external control signal. Relevant control functions, and the measured biomass is sent to external personnel for viewing, thereby enabling online detection of microbial growth.