CN204964400U - Detect total plate count and coliform's quick detecting system of multichannel simultaneously - Google Patents
Detect total plate count and coliform's quick detecting system of multichannel simultaneously Download PDFInfo
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- CN204964400U CN204964400U CN201520665979.4U CN201520665979U CN204964400U CN 204964400 U CN204964400 U CN 204964400U CN 201520665979 U CN201520665979 U CN 201520665979U CN 204964400 U CN204964400 U CN 204964400U
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- coliform
- plate count
- total plate
- light source
- sample
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Abstract
The utility model discloses a detect total plate count and coliform's quick detecting system of multichannel simultaneously. Detecting element's light source and thermostatic control ware are direct continuous with interface circuit board, and photon receiver and electric conductance electrode interface link to each other through AD signal transmission converter and interface circuit board and receive the computer again, and light source and photon receiver are fixed on the support, and the luminous axle of light source and the photic axiform of photon receiver are in alignment, sample test places in the pond in the sharp light path of light source, sample reaction tank one end links together with the sample test pond, and the junction separates with the pellicle, and the other end is furnished with the fixed conductance electrode of ventilative silica gel stopper, the conductance electrode probe stretches into in the sample reaction tank, the computer is used for the conductivity change data determination sample total plate count according to the conductance electrode collection to absorbance change data determination sample coliform according to the collection of photon receiver. This system can be fast, sensitive, accurately detect the total plate count and the coliform of multiple different samples.
Description
Technical field
The utility model relates to the technical field of environment, food and medicine detecting instrument, is specifically related to the multiple tracks rapid detection system for detecting total plate count and coliform simultaneously.
Background technology
In environment, food, medicine hygiene quality inspection, total plate count and coliform are important Hygienic Indexes.In recent decades, China adopts traditional lactose fermentation biochemical identification method to detect coliform always, and the method needs 3-4 days consuming time; Total plate count assay method adopts colony counting method, also needs within 2 days, just can go out result.These two test items nutrient culture media used is not identical with method of operating yet, detect respectively.Also there is the shortcomings such as length consuming time, complex steps, workload be large in traditional detection method.
Therefore be badly in need of a kind of scheme, can quick, sensitive, detect total plate count and coliform exactly simultaneously.
Utility model content
The purpose of this utility model is to provide a kind of multiple tracks rapid detection system simultaneously detecting total plate count and coliform, can detect total plate count and the coliform of multiple different sample quick, sensitive, exactly.
In order to reach above-mentioned purpose, solution of the present utility model is:
Detect the multiple tracks rapid detection system of total plate count and coliform simultaneously, comprise: several detecting units, and the analog digital A/D Signal transmissions converter, Line Card, radiator valve and computing machine that several detecting units described share, it is characterized in that
Several detecting units described contain independently support, example reaction pond, sample detection pond, conductance electrode, light source and photon receiver separately, in several detecting units described light source and the radiator valve of each detecting unit respectively direct and described Line Card be connected, in several detecting units described, the photon receiver of each detecting unit is connected with described Line Card respectively by described A/D Signal transmissions converter with conductance electrode interface, described Line Card is connected to described computing machine, wherein:
Described light source and described photon receiver are separately fixed on described support, the light-emitting axis of described light source and described photon receiver form a straight line by optical axis;
Described sample detection pond is the vial being threaded mouth, is positioned on the linear light path of described light source;
Described example reaction pond is the double glazing post of both ends open, there is screw thread mouth the one end in described example reaction pond, and linked together by the connector of both end thread and described sample detection pond, junction semi-permeable diaphragm separates, and the other end in described example reaction pond is furnished with ventilative silica gel plug;
Described conductance electrode is fixed on the ventilative silica gel plug in described example reaction pond;
Described conductance electrode probe stretches in described example reaction pond;
Described computing machine is used for the conductivity variations data determination sample total plate count gathered according to conductance electrode, and according to the absorbance delta data determination sample coliform that photon receiver gathers.
The material of described semi-permeable diaphragm is regenerated cellulose, and molecular cut off is 1000 dalton.
Described conductance electrode is two electric pole type platinum black conductance electrodes, and electrode constant is 1.0.
Described light source adopts light emitting diode.
Described photon receiver adopts photoelectric tube.
Described radiator valve is made up of temperature control device and heating temperatures device, and described heating temperatures device is connected with described Line Card by described temperature control device.
Several detecting units described are arranged in a casing, and described casing is furnished with lid, are lined with insulation material in the wall of casing, and described radiator valve is arranged in the wall of casing.
After adopting technique scheme, the utility model contains multiple identical independent detection unit, can detect multiple sample simultaneously; The absorbance delta data that the conductivity variations data gathered by conductance electrode and photon receiver are gathered, is realized detecting the total plate count in sample and coliform by the program being solidificated in computing machine simultaneously; By semi-permeable diaphragm, example reaction pond and sample detection pond are separated, the impact on absorbance such as sample particle can be avoided, thus it is wide to make to detect sample type, can detect common various environment, food, drug sample.
In order to be illustrated more clearly in the technical scheme of the utility model embodiment, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model;
Fig. 2 is the structural representation of the utility model detecting unit;
Fig. 3 is circuit diagram of the present utility model.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Fig. 1 is the one-piece construction schematic diagram of the utility model multiple tracks rapid detection system.As shown in Figure 1, multiple tracks rapid detection system can comprise: detecting unit 1, casing 2, lid 3.
Fig. 2 is the structural representation of the detecting unit of the utility model multiple tracks rapid detection system.As shown in Figure 2, detecting unit 1 can comprise: support 4; Sample detection pond 5, connector 6, example reaction pond 7, light source 8, photon receiver 9, semi-permeable diaphragm 10, conductance electrode 11, conductance electrode probe 12, conductance electrode interface 13, ventilative silica gel plug 14.
Fig. 3 is circuit diagram of the present utility model.As shown in Figure 3, circuit can comprise: computing machine 15, Line Card 16, heating temperatures device 17, temperature control device 18, power supply 19, A/D Signal transmissions converter 20.
The utility model contains multiple identical independent detection unit 1, can detect multiple sample simultaneously.Each detecting unit 1 is all containing independently support 4, example reaction pond 7, sample detection pond 5, conductance electrode 11, light source 8 and photon receiver 9.In detecting unit 1, sample detection pond 5 is for being threaded a mouthful vial.Example reaction pond 7 is the double glazing post of both ends open, and wherein there is screw thread mouth one end, and connector 6 and sample detection pond 5 by both end thread link together, and junction semi-permeable diaphragm 10 separates; Example reaction pond 7 other end is furnished with ventilative silica gel plug 14, can effective ventilation, and stops external microbe and enter.Conductance electrode 11 is fixed on the ventilative silica gel plug 14 in described example reaction pond 7, and conductance electrode probe 12 stretches in example reaction pond 7.Light source 8 and photon receiver 9 are separately fixed on support, the light-emitting axis of light source 8 and photon receiver 9 form a straight line by optical axis, 5, sample detection pond is positioned in the light path of straight line.
The power supply 19 of the light source 8 of each detecting unit 1 is connected with Line Card 16 with the temperature control device 18 of radiator valve is direct respectively, heating temperatures device 17 is connected with Line Card 16 by temperature control device 18, Line Card 16 is connected with computing machine 15 again, and the photon receiver 9 of each detecting unit is connected with Line Card 16 respectively by A/D Signal transmissions converter 20 with conductance electrode interface 13.
Several detecting units 1 are arranged in a casing 2, casing are furnished with lid 3, are lined with insulation material in the wall of casing 2, and radiator valve is arranged in the wall of casing 2, and whole casing has the effect of darkroom and constant temperature.
The utility model can realize the total plate count coliform simultaneously detected in sample.The conductivity variations data that computing machine 15 gathers according to conductance electrode 11, the digital signal provided by A/D Signal transmissions converter 20, is gone out the quantity of bacterium in sample by the program computation being solidificated in computing machine 15; According to the absorbance delta data that photon receiver 9 gathers, the digital signal provided by A/D Signal transmissions converter 20, is gone out the quantity of coliform in sample by the program computation being solidificated in computing machine 15.Computing machine 15 can also carry out temperature control and adjustment by radiator valve to whole system.Instrument parameter is arranged by computing machine 15 interface, and according to interface, instrument arranges that requirement completes that light source opens the light, light and electric analoging signal receive, and A/D signal is changed, and automatically resets, the functions such as data autostore.
Technically, the utility model also should be accomplished: conductance electrode probe 12, and light source 8 light emitting diode and photon receiver 9 photoelectric tube will adopt the device that stability is high and consistance is good, make the measuring accuracy between each detecting unit 1 have comparability; Conductance electrode 11, light emitting diode and photoelectric tube power supply control by computing machine 15, and time controling is accurate, improve the accuracy of measuring, can meet the needs of each occasion sample determination such as scientific research, experiment.In addition, system will be placed in darkroom, and darkroom will reach can the requirement of abundant lucifuge.
The utility model can carry out the detection of two project total plate counts that microorganism often examines and coliform simultaneously, highly sensitive, minimumly detects 1/mL(g) sample; Several to dozens of samples can be detected as required simultaneously; Example reaction pond 7 and sample detection pond 5 semi-permeable diaphragm 10 are separated, the compositions such as sample particle can be avoided to have absorbance to affect testing result at 410nm place, thus it is wide to make to detect sample type, can detect common various environment, food, drug sample etc.; Total plate count and coliform metabolism, anticorrosion and Disinfection Effect research etc. can also be used for; Have the advantages that to detect in real time, detection speed is fast, and sample bacteria containing amount is higher, goes out result faster, only needs within 5-17 hour, can provide result; Simple to operate, do not need the microorganism detection operating personnel of complex samples pre-treatment and specialty, not only can save time but also can human resources be saved.The utility model can detect the conventional project that total plate count and this two microorganisms of coliform are often examined simultaneously in a word, have highly sensitive, the feature that sample type is wide, easy and simple to handle, accuracy is high, quick and economic can be detected.
In sample detection pond 5, fill sterilized water when the utility model uses, screw on connector 6, put semi-permeable diaphragm 10 in connector 6 after, screw on example reaction pond 7, example reaction pond 7 and sample detection pond 5 are linked together.Sample and nutrient culture media are placed in example reaction pond 7, then example reaction pond 7 and the connector in sample detection pond 5 are placed on the support 4 of detecting unit 1, cover the lid 14 with conductance electrode 11, conductance electrode is popped one's head in the nutrient culture media in 12 immersion example reaction ponds 7.By computing machine 15, system temperature is set, temperature control and adjustment is carried out by temperature control device 18 pairs of whole systems, system is remained on 36 ± 1 DEG C of applicable total plate count and coliform growth, conductance electrode interface 13 inserts in Line Card 16 plug receptacle, open light source 8, the illumination sent is to sample detection pond 5.When bacterial growth in sample, its physiological metabolism effect makes the electric inert substance of nutrient culture media as carbohydrates, protein and lipid etc., metabolism is the electroactive small-molecule substance of tool, as lactate, acetate etc., these ionic state substances increase the electric conductivity of nutrient culture media, and the conductance of nutrient culture media is changed.By measuring the change of conductance, being carried out the quantity of bacterium in calculation sample by the program being solidificated in computing machine 15, drawing testing result.O-Nitrophenylfluorone-β-D galactoside in the O-Nitrophenylfluorone-β-D galactosidase decomposition nutrient culture media that the coliform in sample produces produces yellow substance, it is 1000 daltonian semi-permeable diaphragms 10 that this yellow substance can pass through molecular cut off, make the water yellowing being contained in detection cell, at 410nm wavelength, absorbance can detect in place.After transmitted light is received by photon receiver 9, by the digital signal that A/D Signal transmissions converter 20 provides, according to the change increasing certain absorbance under 410nm wavelength, carried out the quantity of coliform in calculation sample by the program being solidificated in computing machine 15, draw testing result.
The above; be only embodiment of the present utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; change can be expected easily or replace, all should be encompassed within protection domain of the present utility model.Therefore, protection domain of the present utility model should described be as the criterion with the protection domain of claim.
Claims (7)
1. detect the multiple tracks rapid detection system of total plate count and coliform simultaneously, comprise: several detecting units, and the analog digital A/D Signal transmissions converter, Line Card, radiator valve and computing machine that several detecting units described share, it is characterized in that
Several detecting units described contain independently support, example reaction pond, sample detection pond, conductance electrode, light source and photon receiver separately, in several detecting units described light source and the radiator valve of each detecting unit respectively direct and described Line Card be connected, in several detecting units described, the photon receiver of each detecting unit is connected with described Line Card respectively by described A/D Signal transmissions converter with conductance electrode interface, described Line Card is connected to described computing machine, wherein:
Described light source and described photon receiver are separately fixed on described support, the light-emitting axis of described light source and described photon receiver form a straight line by optical axis;
Described sample detection pond is the vial being threaded mouth, is positioned on the linear light path of described light source;
Described example reaction pond is the double glazing post of both ends open, there is screw thread mouth the one end in described example reaction pond, and linked together by the connector of both end thread and described sample detection pond, junction semi-permeable diaphragm separates, and the other end in described example reaction pond is furnished with ventilative silica gel plug;
Described conductance electrode is fixed on the ventilative silica gel plug in described example reaction pond;
Described conductance electrode probe stretches in described example reaction pond;
Described computing machine is used for the conductivity variations data determination sample total plate count gathered according to conductance electrode, and according to the absorbance delta data determination sample coliform that photon receiver gathers.
2. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, it is characterized in that, the material of described semi-permeable diaphragm is regenerated cellulose, and molecular cut off is 1000 dalton.
3. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, it is characterized in that, described conductance electrode is two electric pole type platinum black conductance electrodes, and electrode constant is 1.0.
4. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, is characterized in that, described light source adopts light emitting diode.
5. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, is characterized in that, described photon receiver adopts photoelectric tube.
6. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, it is characterized in that, described radiator valve is made up of temperature control device and heating temperatures device, and described heating temperatures device is connected with described Line Card by described temperature control device.
7. the multiple tracks rapid detection system simultaneously detecting total plate count and coliform as claimed in claim 1, it is characterized in that, several detecting units described are arranged in a casing, described casing is furnished with lid, be lined with insulation material in the wall of casing, described radiator valve is arranged in the wall of casing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520665979.4U CN204964400U (en) | 2015-08-31 | 2015-08-31 | Detect total plate count and coliform's quick detecting system of multichannel simultaneously |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520665979.4U CN204964400U (en) | 2015-08-31 | 2015-08-31 | Detect total plate count and coliform's quick detecting system of multichannel simultaneously |
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| CN204964400U true CN204964400U (en) | 2016-01-13 |
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| CN201520665979.4U Expired - Fee Related CN204964400U (en) | 2015-08-31 | 2015-08-31 | Detect total plate count and coliform's quick detecting system of multichannel simultaneously |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843624A (en) * | 2017-09-14 | 2018-03-27 | 中国肉类食品综合研究中心 | For detecting the detection means and its detection method of total plate count in food |
| CN112858313A (en) * | 2021-01-14 | 2021-05-28 | 内蒙古农业大学 | Sheep milk adulteration detection device |
| CN114574350A (en) * | 2022-03-03 | 2022-06-03 | 合肥檀泰环保科技有限公司 | System and method for detecting microorganisms in domestic drinking water |
-
2015
- 2015-08-31 CN CN201520665979.4U patent/CN204964400U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843624A (en) * | 2017-09-14 | 2018-03-27 | 中国肉类食品综合研究中心 | For detecting the detection means and its detection method of total plate count in food |
| CN107843624B (en) * | 2017-09-14 | 2020-06-02 | 中国肉类食品综合研究中心 | Detection device and detection method for detecting total number of bacterial colonies in food |
| CN112858313A (en) * | 2021-01-14 | 2021-05-28 | 内蒙古农业大学 | Sheep milk adulteration detection device |
| CN114574350A (en) * | 2022-03-03 | 2022-06-03 | 合肥檀泰环保科技有限公司 | System and method for detecting microorganisms in domestic drinking water |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160113 Termination date: 20160831 |