CN107843624A - For detecting the detection means and its detection method of total plate count in food - Google Patents
For detecting the detection means and its detection method of total plate count in food Download PDFInfo
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- CN107843624A CN107843624A CN201710827061.9A CN201710827061A CN107843624A CN 107843624 A CN107843624 A CN 107843624A CN 201710827061 A CN201710827061 A CN 201710827061A CN 107843624 A CN107843624 A CN 107843624A
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Abstract
The present invention relates to Microbiological detection of foods technical field, discloses a kind of detection means and its detection method for being used to detect total plate count in food, it includes：Reative cell, electric signal amplifier, electric signal data sampling card and electric signal processor, PEM is provided with reative cell, reative cell is divided into anode reaction room and cathode reaction chambers by PEM, and reaction system and electrode are respectively arranged with anode reaction room and cathode reaction room；Electric signal amplifier is connected with the electrode in anode reaction room and cathode reaction room respectively, and electric signal data sampling card is connected with electric signal amplifier and electric signal processor respectively.The detection means and its detection method use electronics caused by electrochemical principle using Microorganism respiration to be used as detection object, the magnitude of voltage and total plate count correlation curve drawn according to concentration known microbial inoculum, the total plate count of sample, the degree of accuracy more than 95% can be obtained within 30 minutes.
Description
Technical field
The present invention relates to Microbiological detection of foods technical field, more particularly to a kind of it is used to detect total plate count in food
Detection means and its detection method.
Background technology
Food microorganisms pollution be influence food security matter of utmost importance, new the law of food safety food is dispatched from the factory before detection
Content has done clear stipulaties, and wherein microorganism especially total plate count is the essential items for inspection before food dispatches from the factory.Bacterium in food at present
Fall sum detection still use colony counting method, exist round of visits grow (generally requiring 2-3 days), complex operation, detection into
A series of problems, such as this is high.The product (such as bean product, meat products) for especially there was only 5-7 days to shelf life, treats microorganism detection
Product can just dispatch from the factory after qualified, on the one hand reduce effective shelf life of product, on the other hand easily cause overstocking for goods, give
Food enterprise causes great circulating pressure and economic loss.It is average because of micro- life according to domestic scale food enterprise is tentatively estimated
Nearly 300,000 yuan/year of direct cost caused by analyte detection, nearly million yuan of indirect expense.Total plate count is quick in food common at present
Detection method includes instant paper disk method, electrical impedance method, Microcolony count method, ATP bioluminescences method and near infrared spectroscopy
Deng, although wherein instant paper disk method, electrical impedance method, Microcolony count method are all high with accuracy, the characteristic of high sensitivity,
Because being required for carrying out microorganism culture breeding during detection, therefore detection time at least needs 7-10 hours；And ATP bioluminescence methods
With the methods of near infrared spectroscopy because Cleaning Principle is different, detection time can foreshorten to 10 minutes even several seconds, but ATP give birth to
Thing fluorescence method is easily disturbed by impurity etc., and near infrared spectrum rule has the problem of model stability deficiency.
The content of the invention
(1) technical problems to be solved
It is an object of the present invention to provide a kind of detection means for detecting total plate count in food to shorten bacterium in food
Fall the detection time of sum, be not easy to be disturbed by factor, and manufacturing cost is relatively low.
It is total to improve bacterium colony in food it is a further object of the present invention to provide a kind of detection method of total plate count in food
Several detection efficiencies.
(2) technical scheme
In order to solve the above-mentioned technical problem, the invention provides a kind of detection dress for being used to detect total plate count in food
Put, it includes：Reative cell, electric signal amplifier, electric signal data sampling card and electric signal processor, set in the reative cell
There is PEM, the reative cell is divided into anode reaction room and cathode reaction chambers by the PEM, in the sun
Reaction system and electrode are respectively arranged with pole reative cell and the cathode reaction room；The electric signal amplifier respectively with it is described
Anode reaction room connects with the electrode in the cathode reaction room, the electric signal data sampling card respectively with the telecommunications
Number amplifier connects with electric signal processor.
Wherein, each composition and its concentration are in the reaction system in the anode reaction room：
Neutral buffered liquid：50mM-100mM,
Matrix：30mM-100mM,
Electronics amplification agent：0.2mM-1.0mM；
Each composition and its concentration are in reaction system in the cathode reaction room：
Neutral buffered liquid：50mM-100mM,
Oxidant：30mM-100mM.
Wherein, the neutral buffered liquid in the reaction system in the anode reaction room is phosphate buffer or Tris-
HCl buffer solutions, the matrix are glucose or sodium acetate；The electronics amplification agent is dimethyl diaminophenazine chloride or methylene blue or 2,6- dichloros
Indophenols sodium.
Wherein, the neutral buffered liquid in the reaction system in the cathode reaction room is phosphate buffer or Tris-
HCl buffer solutions；The oxidant is the potassium ferricyanide or potassium permanganate.
Wherein, the electric signal processor includes：
Electric signal logging modle, for recording the real-time change situation of magnitude of voltage in the reative cell；
Electrical signal data fitting module, it is total for bacterium colony corresponding to the magnitude of voltage that is recorded according to the electric signal logging modle
Number draws both correlation curves；And it is worth to institute's testing sample according to the voltage of the correlation curve and testing sample
Total plate count；
Total plate count output module, for exporting the total plate count of testing sample.
Wherein, the electrode is platinized platinum or graphite.
It is used to detect the detection means of total plate count in food to bacterium in food using above-mentioned the invention also discloses a kind of
Fall the detection method of sum, it comprises the following steps：
S1：The microbial inoculum of a certain amount of multiple known total plate counts is taken to add the reactant of anode reaction room respectively
System, measures corresponding magnitude of voltage, wherein, the known total plate count of multiple microbial inoculums is different；
S2：Total plate count and its corresponding magnitude of voltage according to microbial inoculum, fitting obtain both correlation curves；
S3：Take a certain amount of testing sample homogenate to add the reaction system of anode reaction room, determine the electricity of testing sample
Pressure value；
S4：The magnitude of voltage obtained according to the step S2 correlation curves obtained and step S3, obtains the testing sample
Total plate count.
Wherein, in step s3, the testing sample homogenate is that testing sample presses 1 with physiological saline:10 quality hundred
Divide than the homogenizing fluid after beating homogeneous after mixing.
(3) beneficial effect
The detection means and its detection method provided by the present invention for being used to detect total plate count in food, using electrochemistry
In current potential, electronics caused by current method using Microorganism respiration as detection object, the total plate count microorganism according to known to
The magnitude of voltage and total plate count correlation curve that bacterium solution is drawn, the total plate count of sample can be obtained within 30 minutes, and detected
The degree of accuracy more than 95%.The detection means has high sensitivity, is not easy the advantage disturbed by factor, detection time is short, makes simultaneously
It is relatively low to make cost, it is simple to operate, it is especially suitable for the quick detection of total plate count in food.The detection means is advantageous to promote with method
Enter domestic food enterprise product security level control lifting, extend food enterprise product effective shelf life, reduce product loss,
Food enterprise comprehensive benefit is improved, is also used as field quick detection equipment, improves food safety Regulation personnel's daily hygiene
The efficiency and dynamics of supervision work, provided safeguard for China's food security.
Brief description of the drawings
Fig. 1 is a kind of structural representation for being used to detect the detection means of total plate count in food according to the present invention；
Fig. 2 is a kind of electric signal processing unit for being used to detect the detection means of total plate count in food according to the present invention
Module connection relationship diagram；
In figure, 1：Anode reaction room；2：Cathode reaction chambers；3：Electrode；4：PEM；5：Electric signal amplifier；6：
Electric signal data sampling card；7：Electric signal processor.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Following instance
For illustrating the present invention, but it is not limited to the scope of the present invention.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outer ", " up time
The orientation or position relationship of the instruction such as pin ", " counterclockwise ", " axial direction ", " radial direction ", " circumference " be based on orientation shown in the drawings or
Position relationship, it is for only for ease of and describes the present invention and simplify description, rather than indicates or imply that signified device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the invention, " multiple " are meant that at least two, such as two, three
It is individual etc., unless otherwise specifically defined.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integrally；Can be that machinery connects
Connect or electrically connect or can communicate each other；Can be joined directly together, can also be indirectly connected by intermediary, can be with
It is connection or the interaction relationship of two elements of two element internals, is limited unless otherwise clear and definite.For this area
For those of ordinary skill, the concrete meaning of above-mentioned term in the present invention can be understood as the case may be.
Fig. 1 shows a kind of preferred reality for being used to detect the detection means of total plate count in food according to the present invention
Apply example.As illustrated, the detection means includes：At reative cell, electric signal amplifier 5, electric signal data sampling card 6 and electric signal
Device 7 is managed, is provided with PEM 4 in reative cell, reative cell is divided into anode reaction room 1 and negative electrode anti-by PEM 4
Room 2 is answered, reaction system and electrode 3 are respectively arranged with anode reaction room 1 and cathode reaction chambers 2；Electric signal amplifier 5 is distinguished
It is connected with electrode 3, electric signal data sampling card 6 is connected with electric signal amplifier 5 and electric signal processor 7 respectively.It is in use, logical
Cross PEM 4 and control electronics caused by microbial consumption reaction system, and sun is gathered by electric signal data sampling card
The relative potentials between electrode 3 in pole reative cell 1 and cathode reaction chambers 2 are poor, then by electric signal processor according in advance
The correlation curve of total plate count corresponding to the magnitude of voltage of determination and each microbial inoculum, obtain the magnitude of voltage institute of testing sample
Corresponding total plate count
Specifically, each composition and its concentration are in the reaction system in anode reaction room 1：
Neutral buffered liquid：50mM-100mM,
Matrix：30mM-100mM,
Electronics amplification agent：0.2mM-1.0mM；
Each composition and its concentration are in reaction system in cathode reaction chambers 2：
Neutral buffered liquid：50mM-100mM,
Oxidant：30mM-100mM.
Wherein, the neutral buffered liquid in the reaction system in anode reaction room 1 is that phosphate buffer or Tris-HCl are buffered
Liquid, matrix are glucose or sodium acetate；Electronics amplification agent is dimethyl diaminophenazine chloride or methylene blue or 2,6- sodium dichlorophenol indophenolate, for amplifying
System produces the total amount of electronics.
Neutral buffered liquid in reaction system in cathode reaction chambers 2 is phosphate buffer or Tris-HCl buffer solutions；Oxygen
Agent is the potassium ferricyanide or potassium permanganate, wherein acceptor of the oxidant as electronics, completes the electron transmission of reaction system.
The size of electrode is a 3.0cm*3.0cm*0.1cm, and the preferably electrode with good conductivity such as platinized platinum or graphite.
As shown in Fig. 2 electric signal processing unit is total including electric signal logging modle, electrical signal data fitting module and bacterium colony
Number output module.Electric signal logging modle is used for after recording reative cell addition sample, the real-time change of magnitude of voltage in device loop
Situation, and voltage value data is imported into electrical signal data fitting module.Electrical signal data fitting module, for according to the telecommunications
Total plate count corresponding to the magnitude of voltage of number logging modle record draws both correlation curves；And according to the correlation curve
And the voltage of testing sample is worth to the total plate count of institute's testing sample.Total plate count output module is used to export the telecommunications
The total plate count for the testing sample that number fitting module is calculated.
The invention also discloses a kind of detection method using above-mentioned detection device to total plate count in food, it include with
Lower step：
S1：The microbial inoculum of a certain amount of multiple known total plate counts is taken to add the reactant of anode reaction room respectively
System, measures corresponding magnitude of voltage, wherein, the known total plate count of multiple microbial inoculums is different；
S2：Total plate count and its corresponding magnitude of voltage according to microbial inoculum, fitting obtain both correlation curves；
S3：Take a certain amount of testing sample homogenate to add the reaction system of anode reaction room, determine the electricity of testing sample
Pressure value；
S4：The magnitude of voltage obtained according to the step S2 correlation curves obtained and step S3, obtains the testing sample
Total plate count.
Preferably, in step s3, testing sample homogenate is that testing sample presses 1 with physiological saline (0.9%NaCl):10
Mass percent mixing after pat homogeneous after homogenizing fluid.
The assay method of the present invention is illustrated below by specific embodiment：
The wherein reaction system of anode reaction room is 100ml systems, including 50mM phosphate buffers (pH 7.0), 50mM second
Sour sodium, 0.2mM dimethyl diaminophenazine chlorides；The reaction system of cathode reaction chambers is 100ml systems, including 50mM phosphate buffers (pH 7.0),
50mM potassium permanganate.Electronics caused by microbial consumption sodium acetate is controlled by PEM 4, dimethyl diaminophenazine chloride is used to amplify body
System produces the total amount of electronics；Acceptor of the potassium permanganate as electronics, complete the electron transmission of system.Specific detecting step is as follows：
S1：1 × 10 is taken respectively3CFU/ml、1×104CFU/ml、1×105CFU/ml、1×106CFU/ml、1×107CFU/
Ml microbial inoculum 1ml adds the reaction system of anode reaction room, so using above-mentioned detection device respectively to microbial bacteria
Liquid is measured, and obtains the magnitude of voltage corresponding to each microbial inoculum；
S2：Using the magnitude of voltage corresponding to each microbial inoculum, each bacterium corresponding to drafting magnitude of voltage and microbial inoculum
Fall the correlation curve of sum；
S3：25g live fresh pork samples are put into the homogenizing bag equipped with the physiological saline of 225ml 0.9%, are prepared into homogenate
Liquid.Take 1ml homogenates to add the reaction system of anode reaction room, sample is measured using above-mentioned detection device, acquisition is treated
The magnitude of voltage of test sample product,
S4：The correlation of each total plate count according to corresponding to the magnitude of voltage and microbial inoculum that obtain in step s 2 is bent
Line, obtain the total plate count of live pig fresh meat sample.
The detection means and its detection method provided by the present invention for being used to detect total plate count in food, using electrochemistry
In current potential, electronics caused by current method using Microorganism respiration as detection object, micro- life of total plate count according to known to
The magnitude of voltage and total plate count correlation curve that thing bacterium solution is drawn, the total plate count of sample, detection can be obtained within 30 minutes
Limit 103CFU/g, and accuracy in detection more than 95%.When the detection means has high sensitivity, is not easy to be disturbed by factor, detects
Between short advantage, while cost of manufacture is relatively low, simple to operate, is especially suitable for the quick detection of total plate count in food.The detection
Apparatus and method may advantageously facilitate the lifting of domestic food enterprise product security level control, extend effective goods of food enterprise product
The frame phase, reduce product loss, improve food enterprise comprehensive benefit, being also used as field quick detection equipment, improving food peace
The efficiency and dynamics of full supervisor's daily hygiene supervision work, provide safeguard for China's food security.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (8)
- A kind of 1. detection means for being used to detect total plate count in food, it is characterised in that including：Reative cell, electric signal amplification Device, electric signal data sampling card and electric signal processor, PEM, the PEM are provided with the reative cell The reative cell is divided into anode reaction room and cathode reaction chambers, divided in the anode reaction room and the cathode reaction room Reaction system and electrode are not provided with it；The electric signal amplifier respectively with the anode reaction room and the cathode reaction room Electrode connection, the electric signal data sampling card is connected with the electric signal amplifier and electric signal processor respectively.
- 2. detection means according to claim 1, it is characterised in that in the reaction system in the anode reaction room respectively into Divide and its concentration is：Neutral buffered liquid：50mM-100mM,Matrix：30mM-100mM,Electronics amplification agent：0.2mM-1.0mM；Each composition and its concentration are in reaction system in the cathode reaction room：Neutral buffered liquid：50mM-100mM,Oxidant：30mM-100mM.
- 3. detection means according to claim 2, it is characterised in that the institute in reaction system in the anode reaction room It is phosphate buffer or Tris-HCl buffer solutions to state neutral buffered liquid, and the matrix is glucose or sodium acetate；The electronics is put Big agent is dimethyl diaminophenazine chloride or methylene blue or 2,6 dichlorophenol indophenol sodium.
- 4. detection means according to claim 2, it is characterised in that the institute in reaction system in the cathode reaction room It is phosphate buffer or Tris-HCl buffer solutions to state neutral buffered liquid；The oxidant is the potassium ferricyanide or potassium permanganate.
- 5. detection means according to claim 1, it is characterised in that the electric signal processor includes：Electric signal logging modle, for recording the real-time change situation of magnitude of voltage in the reative cell；Electrical signal data fitting module, painted for total plate count corresponding to the magnitude of voltage that is recorded according to the electric signal logging modle Both correlation curves of system；And it is worth to the bacterium of institute's testing sample according to the voltage of the correlation curve and testing sample Fall sum；Total plate count output module, for exporting the total plate count of testing sample.
- 6. detection means according to claim 1, it is characterised in that the electrode is platinized platinum or graphite.
- 7. a kind of detection means for being used to detect total plate count in food using any one of claim 1-6 is to food The detection method of middle total plate count, it is characterised in that comprise the following steps：S1：Take the microbial inoculum of a certain amount of multiple known total plate counts to add the reaction system of anode reaction room respectively, survey Corresponding magnitude of voltage is obtained, wherein, the known total plate count of multiple microbial inoculums is different；S2：Total plate count and its corresponding magnitude of voltage according to microbial inoculum, fitting obtain both correlation curves；S3：Take a certain amount of testing sample homogenate to add the reaction system of anode reaction room, determine the magnitude of voltage of testing sample；S4：The magnitude of voltage obtained according to the step S2 correlation curves obtained and step S3, obtain the bacterium colony of the testing sample Sum.
- 8. detection method according to claim 7, it is characterised in that in step s3, the testing sample homogenate is Testing sample presses 1 with physiological saline:The homogenizing fluid after homogeneous is patted after 10 mass percent mixing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109504603A (en) * | 2018-10-25 | 2019-03-22 | 江苏省产品质量监督检验研究院 | The detection method of total plate count in a kind of food |
| CN114152980A (en) * | 2021-12-01 | 2022-03-08 | 中国地震局地球物理研究所 | Method and device for rapidly and automatically producing seismic source mechanism solution |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216447A (en) * | 2004-08-02 | 2008-07-09 | 大金工业株式会社 | Oxygen electrode |
| CN102411023A (en) * | 2011-11-01 | 2012-04-11 | 北京绿舍环境能源技术有限责任公司 | Water dissolved oxygen analyzer using proton exchanger membrane and detection method of water dissolved oxygen analyzer |
| CN103364469A (en) * | 2013-05-31 | 2013-10-23 | 内蒙古科技大学 | Device and method for quickly measuring BOD (biochemical oxygen demand) based on microbial electrolysis cell technology |
| CN104458846A (en) * | 2014-12-15 | 2015-03-25 | 重庆大学 | Method for measuring chemical oxygen demand of high-salinity waste water by utilizing microbial fuel cell |
| CN204964400U (en) * | 2015-08-31 | 2016-01-13 | 厦门华厦学院 | Detect total plate count and coliform's quick detecting system of multichannel simultaneously |
| CN106981668A (en) * | 2017-05-04 | 2017-07-25 | 天津天狮学院 | A kind of microorganism electrode and its preparation method and application |
-
2017
- 2017-09-14 CN CN201710827061.9A patent/CN107843624B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216447A (en) * | 2004-08-02 | 2008-07-09 | 大金工业株式会社 | Oxygen electrode |
| CN102411023A (en) * | 2011-11-01 | 2012-04-11 | 北京绿舍环境能源技术有限责任公司 | Water dissolved oxygen analyzer using proton exchanger membrane and detection method of water dissolved oxygen analyzer |
| CN103364469A (en) * | 2013-05-31 | 2013-10-23 | 内蒙古科技大学 | Device and method for quickly measuring BOD (biochemical oxygen demand) based on microbial electrolysis cell technology |
| CN104458846A (en) * | 2014-12-15 | 2015-03-25 | 重庆大学 | Method for measuring chemical oxygen demand of high-salinity waste water by utilizing microbial fuel cell |
| CN204964400U (en) * | 2015-08-31 | 2016-01-13 | 厦门华厦学院 | Detect total plate count and coliform's quick detecting system of multichannel simultaneously |
| CN106981668A (en) * | 2017-05-04 | 2017-07-25 | 天津天狮学院 | A kind of microorganism electrode and its preparation method and application |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109504603A (en) * | 2018-10-25 | 2019-03-22 | 江苏省产品质量监督检验研究院 | The detection method of total plate count in a kind of food |
| CN114152980A (en) * | 2021-12-01 | 2022-03-08 | 中国地震局地球物理研究所 | Method and device for rapidly and automatically producing seismic source mechanism solution |
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