CN101029332A - Bacterium total testing fluid and its testing bottle - Google Patents
Bacterium total testing fluid and its testing bottle Download PDFInfo
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- CN101029332A CN101029332A CN 200610128313 CN200610128313A CN101029332A CN 101029332 A CN101029332 A CN 101029332A CN 200610128313 CN200610128313 CN 200610128313 CN 200610128313 A CN200610128313 A CN 200610128313A CN 101029332 A CN101029332 A CN 101029332A
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Abstract
A bacterium-total test tube liquid and test tube are disclosed. The test liquid consists of protein peptone 5-15 per mill, chloride 3-10 per mill, beef extract 1-5 per mill, glucose 2-5 per mill, distilled water 970-988 per mill and micro-color developing reagent. Height H1 of test liquid: space height H2=1:0.3-0.8. It's fast, convenient, correct and has long storage time. It can be used for water-quality, food, medicines and cosmetics.
Description
One, technical field: the present invention relates to testing method and the Testing apparatus thereof of a kind of bacterium, particularly relate to the test fluid and the test bottle thereof of total plate count in the materials such as a kind of water quality, food, medicine, makeup.
Two, background technology:
Total plate count is meant water quality, food and medicine etc. through handling, after cultivating under certain condition, and the sum of contained bacterial colony in gained 1g or the 1ml sample.Total plate count is mainly as the sign of differentiating contaminated degree such as water quality, food, can use also that this method observes that bacterium breeds in food dynamically so that foundation is provided when test sample carried out hygienical evaluation.
Total plate count is not represented the sum of in esse all bacteriums in the sample, and total plate count can not be distinguished the wherein kind of bacterium, so be called as assorted bacterium number, aerophil number etc. sometimes.
At present, the testing method of total plate count great majority adopt traditional plate count in the sample such as water quality, food, and the key instrument equipment that relates in the plate count is: autoclave sterilizer, oven, biochemical incubator, balance, micro magnifier or colony counter, pH meter or accurate pH test paper, sterilization test tube, plate, calibrated pipet and sampling bottle etc.
The main operational steps of plate count is:
The first step: gather water sample and promptly draw a certain amount of water sample with the sterilization suction pipe with the aseptic technique method;
Second step: the preparation of pure water;
The 3rd step: the dish washing sterilization is about to plate, Erlenmeyer flask, beaker, transfer pipet etc. and puts into autoclave sterilizer, the sterilization certain hour;
The 4th step: culture medium preparation;
The 5th step: inoculation water sample;
The 6th step: cultivate;
The 7th step: enumeration (carrying out in the instrument mode) according to form or color;
The 8th step: calculate.
Traditional plate count is all very strict to laboratory environment and operator's technical requirements, comprises that expertise from Sampling techniques, laboratory condition, plant and instrument, vessel reagent quality, operator, operating skill, practical experience are until all observation processes and sequence of control such as quality control.And schedule of operation is loaded down with trivial details, complicated, tediously long, the error that all might cause test result accidentally of any one link.For example, bacterium is generally the agglomerate aggregate, if the bacterial aggregate aggregate is not fully disperseed in the operating process, then counting is negative error; The throw out that occurs during bacterial growth, particle, bubble etc. are difficult to and the bacterial colony difference sometimes, and then counting is positive error.
Three, summary of the invention:
Purpose of the present invention: overcome the deficiency in the bacterium total testing method in the prior art, provide a kind of quick, easy, have simple and easy bacterium total testing fluid and a test bottle thereof than long pot life.
Technical scheme of the present invention:
The invention provides a kind of bacterium total testing fluid, this test fluid is called the liquid color developing culture medium, is unit with the weight content, by peptone 5～15 ‰, muriate 3～10 ‰, beef extract 1～5 ‰, glucose 2～5 ‰, distilled water 970～988 ‰ and micro-colored indicator are formed.
Muriate is sodium-chlor or Repone K in the described bacterium total testing fluid, and colored indicator is phenol red or toluylene red.
A kind of bottles for testing total number of bacteria, this test bottle is equipped with bacterium total testing fluid, the height H of test fluid in bottle for aseptic, the colourless transparent glass bottle of band plug and aluminium cover in the test bottle
1With the height H of leaving a blank
2Ratio be H
1: H
2=1: 0.3～0.8.
Test fluid in the described bottles for testing total number of bacteria is a unit with the weight content, by peptone 5～15 ‰, and muriate 3～10 ‰, beef extract 1～5 ‰, glucose 2～5 ‰, distilled water 970～988 ‰ and micro-colored indicator are formed.
Test bottle of the present invention can be selected 5～15 milliliters of cillin bottles or oral liquid bottle for use.
The working method of using bacterium total testing fluid of the present invention and test bottle thereof to measure bacterium in the water quality is: with asepsis injector water sample is drawn 1.0 (or 0.5) milliliter, be injected in the test bottle, be positioned over after shaking up in 37 ℃ of loft drier or the water-bath 24 hours, (liquid color is originally as redness in the bottle to judge feminine gender or positive reaction according to the variation of liquid color in the bottle, nondiscoloration is negative, become yellow positive), this is qualitative analysis; According to said method do 2 groups or 3 groups of parallel samples, look into the MPN table, can carry out quantitative test by the stepwise dilution method.Other solid sample such as food then must be with water dissolution or lixiviate, measures as stated above after being processed into liquid again.
Positive beneficial effect of the present invention:
1. test fluid of the present invention is that liquid color developing culture medium sealing is stored in the vial, has than long storage life, save in the plate count second and third, four operation steps, can shorten the detection time more than 1/2, alleviated labour intensity greatly.
2. testing method of the present invention has been exempted aseptic condition necessary in the plate count, makes biochemical investigation to carry out under general environment, thereby can save a large amount of infrastructure investments.
3. opsonigenous substances and test fluid are liquid color developing culture medium generation metachromasia, and be more directly perceived with this basis for estimation as positive reaction, thereby remove the plate count operation steps in the 7th step from; Need not simultaneously to use instruments such as microscope, tester, make the non-specialised staff also can grasp this biochemical investigation technology.
4. bacterium and test fluid are that the feature color reaction of reagent in the liquid color developing culture medium is to carry out automatically in aseptic bacterium test bottle, can avoid pollution and interference in the rapid operating process of traditional method multistep, have significantly reduced operation steps; Reduce the error that causes easily in the operating process, thereby improved the accurate reliability of detected result to a great extent.
5. the present invention can carry out qualitative test to the activated bacterial sum and also can carry out quantitative test, and minimum detectability meets national water quality standard requirement.
6. the present invention can be applicable to the simple and easy quick test of total plate count in the various water quality, and non-specialised staff's number minute can be learned operation.
7. the present invention is loaded on Bacteria Detection technical productsization, commercialization in the clear-glass bottle with pre-configured mensuration fluid-tight, can be directly used in the mensuration of bacterium, carries, easy to use, measure simple fast, accurately, the shelf time is long.Can be used for the bacterium qualitative and quantitative analysis of multiple materials such as water quality, food, medicine, makeup.
Four, description of drawings:
Fig. 1: the structural representation of bottles for testing total number of bacteria
Five, embodiment:
The following examples are only in order to further specify the present invention, rather than limit the scope of the invention.
Embodiment one:
The bacterium total testing fluid prescription is: peptone 5 ‰, and sodium-chlor 10 ‰, beef extract 5 ‰, glucose 5 ‰, distilled water 975 ‰ adds micro-phenol red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 4.5 milliliters every bottle, gets final product through autoclaving.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid
1Be 3 centimetres, the height H of leaving a blank
2It is 1.5 centimetres.
Embodiment two:
The bacterium total testing fluid prescription is: peptone 15 ‰, and sodium-chlor 3 ‰, beef extract 1 ‰, glucose 3 ‰, distilled water 978 ‰ adds micro-toluylene red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 4.5 milliliters every bottle, and autoclaving got final product in 15 minutes.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid
1Be 2 centimetres, the height H of leaving a blank
2It is 1.0 centimetres.
Embodiment three:
The bacterium total testing fluid prescription is: peptone 10 ‰, and Repone K 7 ‰, beef extract 3 ‰, glucose 2 ‰, distilled water 978 ‰ adds micro-phenol red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 9.0 milliliters every bottle, and autoclaving got final product in 15 minutes.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 9.0 milliliters in the present embodiment, the height H of test fluid
1Be 3.5 centimetres, the height H of leaving a blank
2It is 2.8 centimetres.
Embodiment four:
The bacterium total testing fluid prescription is: peptone 5 ‰, and sodium-chlor 3 ‰, beef extract 2 ‰, glucose 2 ‰, distilled water 988 ‰ adds micro-toluylene red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 9.0 milliliters every bottle, gets final product through autoclaving.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 9.0 milliliters in the present embodiment, the height H of test fluid
1Be 4.5 centimetres, the height H of leaving a blank
2It is 2.7 centimetres.
Embodiment five:
The bacterium total testing fluid prescription is: peptone 7 ‰, and Repone K 5 ‰, beef extract 4 ‰, glucose 4 ‰, distilled water 980 ‰ adds micro-phenol red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 4.5 milliliters every bottle, gets final product through autoclaving.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid
1Be 5.0 centimetres, the height H of leaving a blank
2It is 2.0 centimetres.
Embodiment six:
The bacterium total testing fluid prescription is: peptone 12 ‰, and sodium-chlor 3 ‰, beef extract 2 ‰, glucose 3 ‰, distilled water 980 ‰ adds micro-phenol red colored indicator.
Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency phial by 4.5 milliliters every bottle, gets final product through autoclaving.
The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uniform cross section vial, and 4 is bacterium total testing fluid; H
1Be the height of test fluid, H
2Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid
1Be 4 centimetres, the height H of leaving a blank
2It is 1.2 centimetres.
Claims (4)
1. bacterium total testing fluid, this test fluid is called the liquid color developing culture medium, it is characterized in that: be unit with the weight content, by peptone 5～15 ‰, muriate 3～10 ‰, beef extract 1～5 ‰, glucose 2～5 ‰, distilled water 970～988 ‰ and micro-colored indicator are formed.
2. bacterium total testing fluid according to claim 1 is characterized in that: described muriate is sodium-chlor or Repone K, and colored indicator is phenol red or toluylene red.
3. bottles for testing total number of bacteria, this test bottle is characterized in that: bacterium total testing fluid is housed, the height H of test fluid in bottle in the test bottle for aseptic, the colourless transparent glass bottle of band plug and aluminium cover
1With the height H of leaving a blank
2Ratio be H
1: H
2=1: 0.3～0.8.
4. bottles for testing total number of bacteria according to claim 3 is characterized in that: described test fluid is a unit with the weight content, by peptone 5～15 ‰, muriate 3～10 ‰, beef extract 1～5 ‰, glucose 2～5 ‰, distilled water 970～988 ‰ and micro-colored indicator are formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610128313 CN101029332A (en) | 2006-11-30 | 2006-11-30 | Bacterium total testing fluid and its testing bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610128313 CN101029332A (en) | 2006-11-30 | 2006-11-30 | Bacterium total testing fluid and its testing bottle |
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| Publication Number | Publication Date |
|---|---|
| CN101029332A true CN101029332A (en) | 2007-09-05 |
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| CN 200610128313 Pending CN101029332A (en) | 2006-11-30 | 2006-11-30 | Bacterium total testing fluid and its testing bottle |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104046563A (en) * | 2013-03-13 | 2014-09-17 | 长江大学 | Heterotrophic bacteria test bottle production method |
| CN104726377A (en) * | 2015-03-27 | 2015-06-24 | 贵州省轻工业科学研究所 | Meat juice peptone glucose culture medium for producing bacterium |
| CN106868091A (en) * | 2017-04-07 | 2017-06-20 | 滕州合易食品有限公司 | A kind of improved bacterium viable count method |
| CN106932383A (en) * | 2015-12-30 | 2017-07-07 | 华南理工大学 | A kind of bacterium total testing pipe and preparation method thereof |
| CN109439536A (en) * | 2017-08-18 | 2019-03-08 | 黄国仁 | Drink water bacteria fast culture device and total plate count rapid detection system |
-
2006
- 2006-11-30 CN CN 200610128313 patent/CN101029332A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104046563A (en) * | 2013-03-13 | 2014-09-17 | 长江大学 | Heterotrophic bacteria test bottle production method |
| CN104726377A (en) * | 2015-03-27 | 2015-06-24 | 贵州省轻工业科学研究所 | Meat juice peptone glucose culture medium for producing bacterium |
| CN106932383A (en) * | 2015-12-30 | 2017-07-07 | 华南理工大学 | A kind of bacterium total testing pipe and preparation method thereof |
| CN106868091A (en) * | 2017-04-07 | 2017-06-20 | 滕州合易食品有限公司 | A kind of improved bacterium viable count method |
| CN109439536A (en) * | 2017-08-18 | 2019-03-08 | 黄国仁 | Drink water bacteria fast culture device and total plate count rapid detection system |
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