CN201006429Y - Bacteria amount testing bottle - Google Patents

Abstract

The utility model provides a test bottle of bacterial number, which is a germ-free colorless transparent glass bottle provided with a rubber plug and an aluminum cover. The test bottle contains bacterial number test liquid, while the liquid height H<1> and the empty space H<2> are rated as 1:0.3-0.8. The utility model improves bacterial check technique, which packs prepared test liquid into a transparent glass bottle, to be directly used in bacterial test, with portable property, easy operation, simple and quick test, high accuracy, and long storage time. The utility model can be used in qualitative and quantitative test of sulfate reduction bacterial in the water with different qualities.

Description

Bottles for testing total number of bacteria

One, technical field: the utility model relates to a kind of bacterium Testing apparatus, particularly relates to the test bottle of total number of bacteria in the materials such as a kind of water quality, food, medicine, cosmetics.

Two, background technology:

Total number of bacteria is meant water quality, food and medicine etc. through handling, after cultivating under certain condition, and the sum of contained bacterial clump in gained 1g or the 1ml sample.Total number of bacteria is mainly as the sign of differentiating contaminated degree such as water quality, food, can use also that this method observes that bacterium breeds in food dynamically so that foundation is provided when test sample carried out hygienical evaluation.

Total number of bacteria is not represented the sum of in esse all bacteriums in the sample, and total number of bacteria can not be distinguished the wherein kind of bacterium, so be called as assorted bacterium number, aerobic bacteria number etc. sometimes.

At present, the method of testing of total number of bacteria great majority adopt traditional plate count in the sample such as water quality, food, and the key instrument equipment that relates in the plate count is: autoclave sterilizer, oven, biochemical incubator, balance, micro magnifier or colonometer, pH meter or accurate pH test paper, sterilization test tube, plate, measuring pipette and sampling bottle etc.

The main operational steps of plate count is:

The first step: gather water sample and promptly draw a certain amount of water sample with the sterilization suction pipe with the sterile working method;

Second step: the preparation of pure water;

The 3rd step: the dish washing sterilization is about to plate, conical flask, beaker, pipette etc. and puts into autoclave sterilizer, the sterilization certain hour;

The 4th step: culture medium preparation;

The 5th step: inoculation water sample;

The 6th step: cultivate;

The 7th step: colony counting (carrying out in the instrument mode) according to form or color;

The 8th step: calculate.

Traditional plate count is all very strict to laboratory environment and operating personnel's specification requirement, comprises that professional knowledge from Sampling techniques, laboratory condition, instrument and equipment, vessel reagent quality, operating personnel, operative skill, practical experience are until all observation processes and control program such as quality control.And operation sequence is loaded down with trivial details, complicated, tediously long, the error that all might cause test result accidentally of any one link.For example, bacterium is generally the agglomerate aggregation, if the bacterial aggregate aggregation is not fully disperseed in the operating process, then counting is negative error; The sediment that occurs during bacterial growth, particle, bubble etc. are difficult to and the bacterial clump difference sometimes, and then counting is positive error.

Three, utility model content:

The purpose of this utility model: overcome the deficiency in the bacterium total testing method in the prior art, provide a kind of quick, easy, have a simple and easy bottles for testing total number of bacteria than long pot life.

The technical solution of the utility model:

The utility model proposes a kind of bottles for testing total number of bacteria, this test bottle is equipped with bacterium total testing fluid, the height H of test fluid in bottle for aseptic, the colourless transparent glass bottle of band plug and aluminium cover in the test bottle ₁With the height H of leaving a blank ₂Ratio be H ₁: H ₂=1: 0.3～0.8.

Described bottles for testing total number of bacteria, wherein test fluid is unit with the weight content, by peptone 5～15 ‰, chloride 3～10 ‰, beef extract 1～5 ‰, glucose 2～5 ‰, distilled water 970～988 ‰ and micro-colored indicator are formed.

Described bottles for testing total number of bacteria, wherein chloride is sodium chloride or potassium chloride, colored indicator is phenol red or dimethyl diaminophenazine chloride.

Test bottle of the present utility model can be selected 5～15 milliliters of cillin bottles or oral liquid bottle for use.

The method of operating of using the utility model test bottle to measure bacterium in the water quality is: with asepsis injector water sample is drawn 1.0 (or 0.5) milliliter, be injected in the test bottle, be positioned over after shaking up in 37 ℃ of drying boxes or the water-bath 24 hours, (liquid color is originally as redness in the bottle to judge feminine gender or positive reaction according to the variation of liquid color in the bottle, nondiscolouring is negative, become yellow positive), this is qualitative analysis; According to said method do 2 groups or 3 groups of parallel samples, look into the MPN table, can carry out quantitative test by the stepwise dilution method.Other solid sample such as food then must water dissolving or lixiviate, measure as stated above again after being processed into liquid.

Positive beneficial effect of the present utility model:

1. the test fluid in the utility model is that liquid chromogenic culture medium sealing is stored in the vial, has than long storage life, save in the plate count second and third, four operating procedure, can shorten the detection time more than 1/2, alleviated labour intensity greatly.

2. measuring technology of the present utility model has been exempted aseptic condition necessary in the plate count, makes biochemical investigation to carry out under general environment, thereby can save a large amount of infrastructure investments.

3. test fluid generation metachromasia in opsonigenous substance and the test bottle, more directly perceived with this basis for estimation as positive reaction, thus remove the plate count operating procedure in the 7th step from; Need not simultaneously to use instruments such as microscope, tester, make the layman also can grasp this biochemical investigation technology.

4. the feature chromogenic reaction of the test fluid in bacterium and the utility model is to carry out automatically in aseptic bacterium test bottle, can avoid pollution and interference in the rapid operating process of conventional method multistep, has significantly reduced operating procedure; Reduce the error that causes easily in the operating process, thereby improved the accurate reliability of testing result to a great extent.

5. the utility model can carry out qualitative test to the activated bacterial sum and also can carry out quantitative test, and minimum detectability meets national water standard requirement.

6. the utility model can be applicable to the simple and easy quick test of total number of bacteria in the various water quality, and layman's number minute can be learned operation.

7. the utility model is loaded on Bacteria Detection technical productsization, commercialization in the Clear glass bottles and jars with pre-configured mensuration fluid-tight, can be directly used in the mensuration of bacterium, carries, easy to use, measure simple fast, accurately, the holding time is long.Can be used for the bacterium qualitative and quantitative analysis of multiple materials such as water quality, food, medicine, cosmetics.

Four, description of drawings:

Fig. 1: the structural representation of bottles for testing total number of bacteria

Five, the specific embodiment:

The following examples are only in order to further specify the utility model, rather than limit scope of the present utility model.

Embodiment one:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid ₁Be 3 centimetres, the height H of leaving a blank ₂It is 1.5 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 5 ‰, and sodium chloride 10 ‰, beef extract 5 ‰, glucose 5 ‰, distilled water 975 ‰ adds micro-phenol red colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 4.5 milliliters every bottle, gets final product through autoclaving.

Method of operating of the present utility model: water sample is drawn 1.0 (or 0.5) milliliter with asepsis injector, be injected in the test bottle, be positioned over after shaking up in 37 ℃ of drying boxes or the water-bath 24 hours, (liquid color is originally as redness in the bottle to judge feminine gender or positive reaction according to the variation of liquid color in the bottle, nondiscolouring is negative, become yellow positive), this is qualitative analysis; According to said method do 2 groups or 3 groups of parallel samples, look into the MPN table, can carry out quantitative test by the stepwise dilution method.

Embodiment two: no longer narrate with embodiment one something in common, difference is:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid ₁Be 2 centimetres, the height H of leaving a blank ₂It is 1.0 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 15 ‰, and sodium chloride 3 ‰, beef extract 1 ‰, glucose 3 ‰, distilled water 978 ‰ adds micro-dimethyl diaminophenazine chloride colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 4.5 milliliters every bottle, and autoclaving got final product in 15 minutes.

Embodiment three: no longer narrate with embodiment one something in common, difference is:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 9.0 milliliters in the present embodiment, the height H of test fluid ₁Be 3.5 centimetres, the height H of leaving a blank ₂It is 2.8 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 10 ‰, and potassium chloride 7 ‰, beef extract 3 ‰, glucose 2 ‰, distilled water 978 ‰ adds micro-phenol red colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 9.0 milliliters every bottle, and autoclaving got final product in 15 minutes.

Embodiment four: no longer narrate with embodiment one something in common, difference is:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 9.0 milliliters in the present embodiment, the height H of test fluid ₁Be 4.5 centimetres, the height H of leaving a blank ₂It is 2.7 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 5 ‰, and sodium chloride 3 ‰, beef extract 2 ‰, glucose 2 ‰, distilled water 988 ‰ adds micro-dimethyl diaminophenazine chloride colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 9.0 milliliters every bottle, gets final product through autoclaving.

Embodiment five: no longer narrate with embodiment one something in common, difference is:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid ₁Be 5.0 centimetres, the height H of leaving a blank ₂It is 2.0 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 7 ‰, and potassium chloride 5 ‰, beef extract 4 ‰, glucose 4 ‰, distilled water 980 ‰ adds micro-phenol red colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 4.5 milliliters every bottle, gets final product through autoclaving.

Embodiment six: no longer narrate with embodiment one something in common, difference is:

The bottles for testing total number of bacteria implementation method: referring to Fig. 1,1 is aluminium cover, and 2 is plug, and 3 is the uiform section vial, and 4 is bacterium total testing fluid; H ₁Be the height of test fluid, H ₂Be the height of leaving a blank, test bottle is 4.5 milliliters in the present embodiment, the height H of test fluid ₁Be 4 centimetres, the height H of leaving a blank ₂It is 1.2 centimetres.

The bacterium total testing fluid prescription is in the test bottle: peptone 12 ‰, and sodium chloride 3 ‰, beef extract 2 ‰, glucose 3 ‰, distilled water 980 ‰ adds micro-phenol red colored indicator.

Above-mentioned test fluid is prepared the back, and to regulate pH value be 6.8-7.0, is packaged in aseptic, the water white transparency vial by 4.5 milliliters every bottle, gets final product through autoclaving.

Claims (1)

1. bottles for testing total number of bacteria, this test bottle is characterized in that: bacterium total testing fluid is housed, the height H of test fluid in bottle in the test bottle for aseptic, the colourless transparent glass bottle of band plug and aluminium cover ₁With the height H of leaving a blank ₂Ratio be H ₁: H ₂=1: 0.3～0.8.

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