CN100565192C - Hydrogen sulfide production test test fluid and colorimetric testing tube thereof - Google Patents
Hydrogen sulfide production test test fluid and colorimetric testing tube thereof Download PDFInfo
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- CN100565192C CN100565192C CNB2006101283145A CN200610128314A CN100565192C CN 100565192 C CN100565192 C CN 100565192C CN B2006101283145 A CNB2006101283145 A CN B2006101283145A CN 200610128314 A CN200610128314 A CN 200610128314A CN 100565192 C CN100565192 C CN 100565192C
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Abstract
The present invention relates to a kind of fast measuring hydrogen sulfide production test test fluid and colorimetric testing tube thereof.The hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volume mixed preparing form.The hydrogen sulfide production test testing tube contains uniform cross section vacuum seal glass tube, be provided with one section sealing at glass tube one end and be communicated with capillary tube segment, wherein in uniform cross section vacuum seal glass tube, be packaged with the hydrogen sulfide production test test fluid of certain altitude, the height H of test fluid in the vacuum seal glass tube
1With the testing tube uniform cross section height H of leaving a blank
2Ratio be H
1: H
2=1: 5～10.The present invention is with detection technique commercialization, standardization, only need during use in treating test sample, the to fracture capillary tube segment of testing tube, testing sample be in the automatic ration tail pipe with test fluid generation metachromasia, measure simple, fast, need not test under aseptic condition, workload reduces; Testing tube of the present invention, test fluid holding time are long, and use value is better, are easy to promote.
Description
One. technical field:
The present invention relates to a kind of microbial biochemical method of testing and Testing apparatus thereof, particularly relate to a kind of quick test hydrogen sulfide production test test fluid and colorimetric testing tube thereof.
Two, background technology:
Hydrogen sulfide production test is one of microbial biochemical test method of using always, its principle is: some bacterium can reduce sodium thiosulfate in the nutrient solution or sodium sulphate, sodium sulphite and generate sulfuretted hydrogen, and the feminine gender of sulfuretted hydrogen or positive reaction can be used as the discriminating foundation to different bacterium.For example: detection of Salmonella, Citrobacter freundii, slowly Edwardsiella, Le Kelai Pseudomonas and the positive reaction of Proteus, the negative reaction of other Pseudomonas.
At present, in the microbial biochemical test, the conventional test methodologies of hydrogen sulfide production test is to add ferrous sulphate or Ferric Ammonium Citrate in nutrient culture media, get bacterium pure culture to be checked, with the transfer needle percutaneous puncture-inoculation in nutrient culture media, under 36 ℃ of conditions, cultivated 24～48 hours, generate the iron protosulfide precipitation of black with sulfuretted hydrogen and judge.But there is following some problem in this kind hydrogen sulfide production test conventional test methodologies:
1. the sensitivity of this hydrogen sulfide production test method of testing is lower, could form black precipitate when the sulfuretted hydrogen of generation must reach higher concentration, and then judge.
2. this method of testing requires comparatively strictness to operating personnel's professional technique, and must operate under strict aseptic environments condition, and the influence factor that relates in the operating process is more, is prone to false negative or weak positive reaction.
3. this method of testing required time is longer, and only the test duration just needs 24～48 hours generally speaking; Still do not comprise vessel sterilization, sterilization preparation routine and post processor.
Three, summary of the invention:
Purpose of the present invention: at the deficiency that exists in the hydrogen sulfide production test method of testing in the background technology, for improving the sensitivity of hydrogen sulfide production test test, simplify testing procedure, accelerate test speed, the present invention proposes a kind of hydrogen sulfide production test test fluid and colorimetric testing tube thereof.
The principle of hydrogen sulfide production test method of testing of the present invention be sulfuretted hydrogen when under acid condition, having molysite to exist and right-amino-dimethyl benzene amine salt reaction generate the complex compound of blue look.
Technical scheme of the present invention is:
The invention provides a kind of hydrogen sulfide production test test fluid, by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volume mixed preparing form.
Described hydrogen sulfide production test test fluid, by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 15～20 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test test fluid, by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 10～15 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test test fluid, by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 20～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Right in the described hydrogen sulfide production test test fluid-amino-dimethyl benzene amine salt is right-amino-dimethyl benzene amine hydrochlorate, or is right-amino-dimethyl benzene amine sulfate; Described molysite is a ferric trichloride, or is ammonium ferric sulfate.
A kind of hydrogen sulfide production test colorimetric testing tube, be vacuum sealing tube, contain uniform cross section vacuum seal glass tube, be provided with one section sealing at glass tube one end and be communicated with capillary tube segment, in uniform cross section vacuum seal glass tube, be packaged with the hydrogen sulfide production test test fluid of certain altitude, the height H of test fluid
1With the testing tube uniform cross section height H of leaving a blank
2Ratio be H
1: H
2=1: 5～10; Described hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volume mixed preparing form.
Described hydrogen sulfide production test colorimetric testing tube, wherein the hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 15～20 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein the hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～15 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein the hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 20～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Above-mentioned hydrogen sulfide production test colorimetric testing tube, right in the described hydrogen sulfide production test test fluid-amino-dimethyl benzene amine salt is right-amino-dimethyl benzene amine hydrochlorate, or is right-amino-dimethyl benzene amine sulfate; Described molysite is a ferric trichloride, or is ammonium ferric sulfate.
Positive beneficial effect of the present invention:
1. the present invention is packaged in the glass tube with vacuum test fluid for preparing standby, capillary tube segment fractures during use, sample liquid automatic ration charges in the testing tube, test fluid is fully mixed with sample liquid, whether question response back observation color fully changes, if the color no change still is the then negative reaction of yellow,, can judge thus whether certain bacterium exists if become the then positive reaction of blue-green.Product detection sensitivity height of the present invention, only needing to produce 0.1mg/L sulfuretted hydrogen can detect, can realize fast detecting, disturbing factor is few, use simply, simplify greatly and detect step, the test duration was reduced to 6～10 hours by original 24～48 hours, alleviated labour intensity to a great extent, testing result accurately and reliably.
2. compare with traditional hydrogen sulfide production test method of testing, the present invention is with detection technique commercialization, standardization, need not when each test, to prepare relevant reagent, the capillary tube segment of the testing tube that only need in determinand, fracture during use, determinand is to react with test fluid in the automatic ration tail pipe.The present invention is simple to operate, quick; And need not under aseptic condition, test, need not carry out vessel sterilization preparation routine and post processor, workload is low; Testing tube, test fluid holding time are long, are easy to promote the practical value height.
3. the chromogenic reaction of test fluid of the present invention and determinand is to carry out automatically in the glass tube with vacuum under specific acid condition, can avoid stochastic error or personal error effectively, helps improving accuracy of detection.
The present invention carry, easy to use, carry out the scene and detect and not need miscellaneous equipment instrument and sterile working platform, economize on the use of funds, save human and material resources, reduce integrated cost.The present invention is easy to promotion and implementation, has good social and economic benefit.
Four, description of drawings:
Fig. 1: hydrogen sulfide production test colorimetric testing tube structural representation
Five, embodiment:
The following examples are only in order to further specify the present invention, rather than limit the scope of the invention.
Embodiment one:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, 10 parts of 6% sulfuric acid solutions and 4 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6 millimeters in the present embodiment, 13.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 2 centimetres, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: with the unit volume example, and by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, 10 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% liquor ferri trichloridi.
Embodiment two:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 2 parts in 2% pair-amino-dimethyl benzene amine sulfate, 25 parts of 6% sulfuric acid solutions and 6 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 7.5 millimeters in the present embodiment, 12 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 9 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 25 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% liquor ferri trichloridi.
Embodiment three:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, 18 parts of 6% sulfuric acid solutions and 5 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 8 millimeters in the present embodiment, 11.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 8 centimetres, capillary tube segment length is 2.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 18 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment four:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 15 parts of 6% sulfuric acid solutions and 5 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 13 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1.6 centimetres, the height H of leaving a blank
2Be 9.6 centimetres, capillary tube segment length is 1.8 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 15 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment five:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 20 parts of 6% sulfuric acid solutions and 5 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 9 millimeters in the present embodiment, 10 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 7 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 20 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment six:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 18 parts of 6% sulfuric acid solutions and 4 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 12.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 18 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% liquor ferri trichloridi.
Embodiment seven:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 15 parts of 6% sulfuric acid solutions and 6 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6 millimeters in the present embodiment, 13.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 2 centimetres, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: with the unit volume example, and by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 15 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% liquor ferri trichloridi.
Embodiment eight:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, 12 parts of 6% sulfuric acid solutions and 5 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 7.5 millimeters in the present embodiment, 12 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 9 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 12 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% liquor ferri trichloridi.
Embodiment nine:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1 part in 2% pair-amino-dimethyl benzene amine sulfate, 15 parts of 6% sulfuric acid solutions and 4 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 8 millimeters in the present embodiment, 11.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 8 centimetres, capillary tube segment length is 2.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1 part in 2% pair-amino-dimethyl benzene amine sulfate, and 15 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment ten:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 20 parts of 6% sulfuric acid solutions and 6 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 13 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1.6 centimetres, the height H of leaving a blank
2Be 9.6 centimetres, capillary tube segment length is 1.8 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 20 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment 11:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, 20 parts of 6% sulfuric acid solutions and 4 parts of mixed preparing of 10% ammonium ferric sulfate solution form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 9 millimeters in the present embodiment, 10 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 7 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, and 20 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment 12:
Hydrogen sulfide production test test fluid prescription is: be example with the unit volume, by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 22 parts of 6% sulfuric acid solutions and 5 parts of mixed preparing of 10% liquor ferri trichloridi form.
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 12.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 22 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% liquor ferri trichloridi.
Claims (9)
1. hydrogen sulfide production test test fluid, it is characterized in that: by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volume mixed preparing form.
2. hydrogen sulfide production test test fluid according to claim 1 is characterized in that: by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 15～20 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
3. hydrogen sulfide production test test fluid according to claim 1 is characterized in that: by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 10～15 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
4. hydrogen sulfide production test test fluid according to claim 1 is characterized in that: by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, 6% sulfuric acid solution, 20～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
5. according to each described hydrogen sulfide production test test fluid of claim 1～4, it is characterized in that: described right-the amino-dimethyl benzene amine salt is right-amino-dimethyl benzene amine hydrochlorate, or is right-amino-dimethyl benzene amine sulfate; Described molysite is a ferric trichloride, or is ammonium ferric sulfate.
6. hydrogen sulfide production test colorimetric testing tube, be vacuum sealing tube, contain uniform cross section vacuum seal glass tube, be provided with one section sealing at glass tube one end and be communicated with capillary tube segment, it is characterized in that: in uniform cross section vacuum seal glass tube, be packaged with the hydrogen sulfide production test test fluid of certain altitude, the height H of test fluid
1With the testing tube uniform cross section height H of leaving a blank
2Ratio be H
1: H
2=1: 5～10; Described hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volume mixed preparing form.
7. hydrogen sulfide production test colorimetric testing tube according to claim 6, it is characterized in that: described hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 15～20 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
8. hydrogen sulfide production test colorimetric testing tube according to claim 6, it is characterized in that: described hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～15 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
9. hydrogen sulfide production test colorimetric testing tube according to claim 6, it is characterized in that: described hydrogen sulfide production test test fluid is by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 20～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
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| CN102313733B (en) * | 2010-07-05 | 2013-07-31 | 中国石油集团川庆钻探工程有限公司川东钻探公司 | Method for rapid testing hydrogen sulfide content in drilling fluid |
| CN102507552B (en) * | 2011-10-24 | 2013-06-12 | 北京市药品检验所 | Method for rapidly detecting tadalafil or derivatives thereof |
| CN103411899B (en) * | 2013-05-15 | 2016-03-02 | 中国科学院烟台海岸带研究所 | Sulfuretted hydrogen kit in sensitive on-the-spot colorimetric detection air and detection method thereof |
| CN103776786A (en) * | 2014-02-22 | 2014-05-07 | 云南健牛生物科技有限公司 | Method for detecting hydrogen sulfide in blood and urine |
| CN107037041B (en) * | 2016-02-03 | 2020-04-21 | 中国科学院大连化学物理研究所 | Promote H2Method for detecting sensitivity of S gas sensor |
| CN109946249B (en) * | 2019-03-18 | 2021-06-01 | 江苏科技大学 | Method for colorimetric determination of hydrogen sulfide by using headspace single-drop liquid phase microextraction method and intelligent equipment |
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Non-Patent Citations (2)
| Title |
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| 亚甲基蓝比色法测定大气中的硫化氢. 龙金满.云南环境科学 增刊，第22卷. 2003 |
| 亚甲基蓝比色法测定大气中的硫化氢. 龙金满.云南环境科学 增刊，第22卷. 2003 * |
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Assignee: ZHENGZHOU WATER TESTING TECHNOLOGY CO., LTD. Assignor: Bai Li Contract record no.: 2012410000012 Denomination of invention: Liquid for testing hydrogen sulfide and its colorimetric testing tube Granted publication date: 20091202 License type: Exclusive License Open date: 20070905 Record date: 20120306 |
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