CN201007706Y - Colour comparison detecting tube of hydrogen sulfide test - Google Patents
Colour comparison detecting tube of hydrogen sulfide test Download PDFInfo
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- CN201007706Y CN201007706Y CNU200620134861XU CN200620134861U CN201007706Y CN 201007706 Y CN201007706 Y CN 201007706Y CN U200620134861X U CNU200620134861X U CN U200620134861XU CN 200620134861 U CN200620134861 U CN 200620134861U CN 201007706 Y CN201007706 Y CN 201007706Y
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- hydrogen sulfide
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Abstract
The utility model relates to a color comparison detecting tube for rapid detection of hydrogen sulfide test. The color comparison detecting tube for hydrogen sulfide test includes a uniform cross-section and vacuum tightness glass tube. One end of the glass tube is provided with a section of sealed and communicating capillary. Testing fluid for hydrogen sulfide test with some height is sealed inside the color comparison detecting tube for hydrogen sulfide test. The ratio between testing fluid height H1 of the uniform cross-section and vacuum tightness glass tube and the remaining-space height H2 is H1: H2 =1:5-10.The utility model productizes and standardizes testing technology. When the utility model is in usage, the only need is to break the capillary of the testing tube, and then samples ready for testing are automatically and quantitatively sucked to produce color bleeding reaction. The testing is simple, rapid, without need of testing in sterility condition, and the workload is low. The testing tube of the utility model has the advantages of long storage time, economy of testing time, better use-value and easy publicity.
Description
One. technical field: the utility model relates to a kind of microbial biochemical Testing apparatus, particularly relates to a kind of quick test hydrogen sulfide production test colorimetric testing tube.
Two, background technology:
Hydrogen sulfide production test is one of microbial biochemical test method of using always, its principle is: some bacterium can reduce sodium thiosulfate in the nutrient solution or sodium sulphate, sodium sulphite and generate sulfuretted hydrogen, and the feminine gender of sulfuretted hydrogen or positive reaction can be used as the discriminating foundation to different bacterium.For example: detection of Salmonella, Citrobacter freundii, slowly Edwardsiella, Le Kelai Pseudomonas and the positive reaction of Proteus, the negative reaction of other Pseudomonas.
At present, in the microbial biochemical test, the conventional test methodologies of hydrogen sulfide production test is to add ferrous sulphate or Ferric Ammonium Citrate in nutrient culture media, get bacterium pure culture to be checked, with the transfer needle percutaneous puncture-inoculation in nutrient culture media, under 36 ℃ of conditions, cultivated 24～48 hours, generate the iron protosulfide precipitation of black with sulfuretted hydrogen and judge.But there is following some problem in this kind hydrogen sulfide production test conventional test methodologies:
1. the sensitivity of this hydrogen sulfide production test method of testing is lower, could form black precipitate when the sulfuretted hydrogen of generation must reach higher concentration, and then judge.
2. this method of testing requires comparatively strictness to operating personnel's professional technique, and must operate under strict aseptic environments condition, and the influence factor that relates in the operating process is more, is prone to false negative or weak positive reaction.
3. this method of testing required time is longer, and only the test duration just needs 24～48 hours generally speaking, does not still comprise vessel sterilization, sterilization preparation routine and post processor.
Three, utility model content:
The purpose of this utility model: at the deficiency in the background technology,, simplify testing procedure, accelerate test speed, the utility model proposes a kind of hydrogen sulfide production test colorimetric testing tube for improving the sensitivity of hydrogen sulfide production test test.
The technical solution of the utility model is:
The utility model provides a kind of hydrogen sulfide production test colorimetric testing tube, be vacuum sealing tube, contain uniform cross section vacuum seal glass tube, be provided with one section sealing at glass tube one end and be communicated with capillary tube segment, in uniform cross section vacuum seal glass tube, be packaged with the hydrogen sulfide production test test fluid of certain altitude, the height H of test fluid
1With the testing tube uniform cross section height H of leaving a blank
2Ratio be H
1: H
2=1: 5～10.
Described hydrogen sulfide production test colorimetric testing tube, wherein said hydrogen sulfide production test test fluid are by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein said hydrogen sulfide production test test fluid are by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 15～20 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein said hydrogen sulfide production test test fluid are by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 10～15 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein said hydrogen sulfide production test test fluid are by 2% pair-amino-dimethyl benzene amine salt, 1～2 unit volume, and 6% sulfuric acid solution, 20～25 unit volumes and 10% iron salt solutions, 4～6 unit volumes are formed.
Described hydrogen sulfide production test colorimetric testing tube, wherein said right-the amino-dimethyl benzene amine salt is right-amino-dimethyl benzene amine hydrochlorate, or is right-amino-dimethyl benzene amine sulfate; Described molysite is a ferric trichloride, or is ammonium ferric sulfate.
Positive beneficial effect of the present utility model:
1. the utility model is packaged in the glass tube with vacuum test fluid for preparing standby, capillary tube segment fractures during use, sample liquid automatic ration charges in the testing tube, test fluid is fully mixed with sample liquid, whether question response back observation color fully changes, if the color no change still is the then negative reaction of yellow,, can judge thus whether certain bacterium exists if become the then positive reaction of blue-green.The utility model product detection sensitivity height, only needing to produce 0.1mg/L sulfuretted hydrogen can detect, can realize fast detecting, disturbing factor is few, use simply, simplify greatly and detect step, the test duration was reduced to 6～10 hours by original 24～48 hours, alleviated labour intensity to a great extent, testing result accurately and reliably.
2. compare with traditional hydrogen sulfide production test method of testing, the utility model is with detection technique commercialization, standardization, need not when each test, to prepare relevant reagent, only need during use to fracture in the determinand capillary tube segment of testing tube, determinand is to react with test fluid in the automatic ration tail pipe.The utility model is simple to operate, quick; And need not under aseptic condition, test, need not carry out vessel sterilization preparation routine and post processor, workload is low; Testing tube, test fluid holding time are long, are easy to promote the practical value height.
3. the chromogenic reaction of the utility model test fluid and determinand is to carry out automatically in the glass tube with vacuum under specific acid condition, can avoid stochastic error or personal error effectively, helps improving accuracy of detection.
The utility model carry, easy to use, carry out the scene and detect and not need miscellaneous equipment instrument and sterile working platform, economize on the use of funds, save human and material resources, reduce integrated cost.The utility model is easy to promotion and implementation, has good social and economic benefit.
Four, description of drawings:
Fig. 1: hydrogen sulfide production test colorimetric testing tube structural representation
Five, embodiment:
The following examples are only in order to further specify the utility model, rather than limit scope of the present utility model.
Embodiment one:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6 millimeters in the present embodiment, 13.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 2 centimetres, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
When the utility model is practical, the capillary tube segment of testing tube is immersed in the analyte sample fluid, capillary tube segment fractures, sample liquid automatic ration charges in the testing tube, test fluid is fully mixed with sample liquid, and whether question response back observation color fully changes, if the color no change still is the then negative reaction of yellow, if become the then positive reaction of blue-green, can judge thus whether certain bacterium exists.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: with the unit volume example, and by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, 10 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% liquor ferri trichloridi.
Embodiment two: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 7.5 millimeters in the present embodiment, 12 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 9 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 25 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% liquor ferri trichloridi.
Embodiment three: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 8 millimeters in the present embodiment, 11.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 8 centimetres, capillary tube segment length is 2.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 18 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment four: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 13 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1.6 centimetres, the height H of leaving a blank
2Be 9.6 centimetres, capillary tube segment length is 1.8 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 15 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment five: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 9 millimeters in the present embodiment, 10 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 7 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 20 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment six: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 12.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 18 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% liquor ferri trichloridi.
Embodiment seven: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6 millimeters in the present embodiment, 13.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 2 centimetres, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: with the unit volume example, and by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, 15 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% liquor ferri trichloridi.
Embodiment eight: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 7.5 millimeters in the present embodiment, 12 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 9 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts in 2% pair-amino-dimethyl benzene amine sulfate, and 12 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% liquor ferri trichloridi.
Embodiment nine: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 8 millimeters in the present embodiment, 11.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 8 centimetres, capillary tube segment length is 2.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1 part in 2% pair-amino-dimethyl benzene amine sulfate, and 15 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment ten: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 13 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1.6 centimetres, the height H of leaving a blank
2Be 9.6 centimetres, capillary tube segment length is 1.8 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 2 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 20 parts of 6% sulfuric acid solutions and 6 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment 11: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 9 millimeters in the present embodiment, 10 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 7 centimetres, capillary tube segment length is 2 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1 part of 2% pair-amino-dimethyl benzene amine hydrochlorate, and 20 parts of 6% sulfuric acid solutions and 4 parts of compositions of 10% ammonium ferric sulfate solution.
Embodiment 12: no longer narrate with embodiment one something in common, difference is:
Hydrogen sulfide production test colorimetric testing tube implementation method: referring to Fig. 1,1 is the uniform cross section glass tube with vacuum among the figure, and 2 is capillary tube segment, and 3 is the hydrogen sulfide production test test fluid.H
1Be test fluid height, H
2Leave a blank highly for the uniform cross section vacuum tube, uniform cross section glass tube with vacuum external diameter is 6.5 millimeters in the present embodiment, 12.5 centimetres of overall heights, the height H of glass tube build-in test liquid
1Be 1 centimetre, the height H of leaving a blank
2Be 10 centimetres, capillary tube segment length is 1.5 centimetres.
Hydrogen sulfide production test test fluid prescription is in the present embodiment testing tube: by 1.5 parts of 2% pair-amino-dimethyl benzene amine hydrochlorates, and 22 parts of 6% sulfuric acid solutions and 5 parts of compositions of 10% liquor ferri trichloridi.
Claims (1)
1. hydrogen sulfide production test colorimetric testing tube, be vacuum sealing tube, contain uniform cross section vacuum seal glass tube, be provided with one section sealing at glass tube one end and be communicated with capillary tube segment, it is characterized in that: in uniform cross section vacuum seal glass tube, be packaged with the hydrogen sulfide production test test fluid of certain altitude, the height H of test fluid
1With the testing tube uniform cross section height H of leaving a blank
2Ratio be H
1: H
2=1: 5～10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200620134861XU CN201007706Y (en) | 2006-11-30 | 2006-11-30 | Colour comparison detecting tube of hydrogen sulfide test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200620134861XU CN201007706Y (en) | 2006-11-30 | 2006-11-30 | Colour comparison detecting tube of hydrogen sulfide test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201007706Y true CN201007706Y (en) | 2008-01-16 |
Family
ID=39003653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU200620134861XU Expired - Fee Related CN201007706Y (en) | 2006-11-30 | 2006-11-30 | Colour comparison detecting tube of hydrogen sulfide test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201007706Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932383A (en) * | 2015-12-30 | 2017-07-07 | 华南理工大学 | A kind of bacterium total testing pipe and preparation method thereof |
-
2006
- 2006-11-30 CN CNU200620134861XU patent/CN201007706Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932383A (en) * | 2015-12-30 | 2017-07-07 | 华南理工大学 | A kind of bacterium total testing pipe and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080116 Termination date: 20091230 |