CN106932383A - A kind of bacterium total testing pipe and preparation method thereof - Google Patents
A kind of bacterium total testing pipe and preparation method thereof Download PDFInfo
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- CN106932383A CN106932383A CN201511015810.5A CN201511015810A CN106932383A CN 106932383 A CN106932383 A CN 106932383A CN 201511015810 A CN201511015810 A CN 201511015810A CN 106932383 A CN106932383 A CN 106932383A
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- glass tube
- blotting paper
- glass
- total testing
- bacterium total
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- 238000012360 testing method Methods 0.000 title claims abstract description 39
- 241000894006 Bacteria Species 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000011521 glass Substances 0.000 claims abstract description 61
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 23
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 239000004568 cement Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 241000206575 Chondrus crispus Species 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 6
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 5
- 238000002513 implantation Methods 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 229920002050 silicone resin Polymers 0.000 claims description 5
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical group [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 229920001131 Pulp (paper) Polymers 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 8
- 239000000499 gel Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 235000012206 bottled water Nutrition 0.000 description 4
- 238000004500 asepsis Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- LCJINPXTMHVZGV-UHFFFAOYSA-N 2,3,5-triphenyl-1h-tetrazol-4-ium;chloride Chemical group [Cl-].[NH2+]1N(C=2C=CC=CC=2)N(C=2C=CC=CC=2)N=C1C1=CC=CC=C1 LCJINPXTMHVZGV-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000012376 hot air sterilization Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of bacterium total testing pipe, including glass tube, the blotting paper being placed in the glass tube, the sealing ring that is sealingly disposed in the glass tube two ends, nutrient solution, indicator and cold water gel are adsorbed with the blotting paper.Present invention also offers a kind of preparation method of bacterium total testing pipe.The present invention prepare simple, low cost, small volume, it is easy to carry, using simple, dependable performance, false drop rate be low, preservation is convenient, can wide popularization and application.
Description
Technical field
The present invention relates to a kind of instrument detected to total number of bacteria in water, more particularly to a kind of bacterium total testing pipe and preparation method thereof.
Background technology
Total number of bacteria as the microorganisms in water Pollution such as running water, bottled water, bottled water optimumitem, there is its important meaning drinking water quality secure context is evaluated, degree of the water body by pollutions such as sanitary sewage, industrial and agricultural wastewater, humans and animals excrement can be assessed.The detection of drinking water total plate count has been used up plate count in GB, and process is cumbersome and has requirement higher to operating personnel.2008, test piece method was written with GB food hygiene Micro biological Tests as the second method of total plate count.The method can reduce early stage and prepare the extensive work such as culture medium and later stage cleaning, improve operating efficiency.Can avoid again with plate count due to the too high inactivation of bacteria for causing of the nutrient agar temperature poured into.
But being carried out on smooth table top using needs for bacterium total testing piece, typically can not directly use out of doors, this requires that detected sample must bring laboratory, so as to produce the time to be measured.Time to be measured is more long, and the degree of flase drop is bigger, because most of bacterium can breed a generation in 20 minutes.In order to reduce the degree of flase drop, the degree of accuracy of detection is improved, reduce some detection disputes, the present invention provides a kind of bacterium total testing pipe, detected at once after can making water sample sampling, realizes for zero time to be measured, eliminates flase drop.
The content of the invention
For the demand and related technological difficulties of the detection of current total number of bacteria, present invention offer is a kind of can Direct Analysis are detected after spot sampling bacterium total testing pipe and preparation method thereof.This testing tube can detect the total number of bacteria in the water bodys such as running water, bottled water, bottled water, and simple to operate, accuracy in detection is high, dependable performance, be particularly suited for outdoor water body detection, can wide popularization and application.
The present invention is adopted the following technical scheme that：
A kind of bacterium total testing pipe, including glass tube, the blotting paper being placed in the glass tube, the sealing ring that is sealingly disposed in the glass tube two ends, are adsorbed with nutrient solution, indicator and cold water gel in the blotting paper.
Further, the glass tube internal diameter is the hollow simple glass pipe of 5-10mm.
Further, the blotting paper is stronger using filter paper, lens wiping paper, fibrous absorbent paper, wood pulp fluff, or other water imbibitions, the paper with certain degree of hardness Yu toughness.
Further, by the resilient sealing ring of tool that is formed in glass cement implantation glass pipe, the glass cement can be sealing neutral silicone resin to the sealing ring.
Further, the indicator is 2,3,5- triphenyl tetrazolium chloride indicator.
Further, the cold water gel is carragheen, Sodium Polyacrylate.
A kind of preparation method of the pipe of bacterium total testing as described, including step：
1）Prepare nutrient solution, indicator and cold water gel mixed liquor：Peptone, yeast extract, glucose, dipotassium hydrogen phosphate, sodium chloride, 2,3,5- triphenyl tetrazolium chloride indicator is added to be respectively 2.590g in per 100mL sterile pure waters, 1.050g, 0.239g, 0.250g, 0.450g, 0.004g, adds carragheen to be respectively 1.000g, 0.300g with Sodium Polyacrylate；
2）The blotting paper of clip suitable dimension, in immersion mixed liquor, after being picked up with tweezers, lies on pallet, is subsequently placed in drying in baking oven；
3）One section of suitable glass tube of interception, internal diameter is about 5-10mm, length is about 20-25cm, during blotting paper after drying is filled in glass tube, the width of blotting paper is just long with glass tube inner circumferential identical, blotting paper is become a circle, is close on glass tube inner side, blotting paper require the length 1cm shorter than glass tube of blotting paper in the equal retraction 0.5cm in glass tube two ends；
4）In glass tube two ends implantation glass glue, thickness is 0.5cm, then stands 20-24 h, allows glass cement to solidify to form sealing ring；
5）The testing tube for preparing is put into baking oven, discontinuous sterilization is carried out, sterilising temp is 70 ± 5 DEG C, and number of times is 3 times, every time after sterilizing, after room temperature places 20-24 h, then carry out sterilizing next time.
Further, step 2）Described in baking oven drying temperature be less than 60 DEG C.
Further, step 2）Described in immersion mixed liquor, after being picked up with tweezers, lie on pallet, be subsequently placed in the process dried in baking oven and repeat 2-3 times.
The bacterium total testing pipe that the present invention is provided includes glass tube, blotting paper, sealing ring.Preparation process is：The blotting paper for speckling with nutrient solution, indicator and cold water gel is dried, is then filled in simple glass pipe, glass tube two ends are sealed with glass cement.After Deng glass cement solidification, glass tube is placed in after carrying out hot air sterilization in baking oven, that is, product is obtained.When using, 0.5mL or 1mL water samples are taken with asepsis injector, slowly injected in testing tube, allow water sample to stick on blotting paper.
Compared to existing technology, the present invention provides this bacterium total testing pipe and preparation method thereof and has the following advantages that：
1, this testing tube is prepared simply, and low cost, small volume is easy to carry, dependable performance, is preserved in normal temperature.
2, this testing tube uses simple, it is only necessary to injected in testing tube with asepsis injector water sampling.Treat that water sample is fixed on blotting paper, then testing tube is put into incubator by relevant criterion method culture time enough, take out several red points.
3, after being finished, after removing sealing ring with physical method, glass tube is recycling.
4, preparation process is simple and easy to apply.
Brief description of the drawings
Fig. 1 is the structural representation of the bacterium total testing pipe of the embodiment of the present invention.
Fig. 2 is the schematic cross-section of Fig. 1.
It is shown in figure：1 --- sealing ring；2 --- glass tube；3 --- blotting paper.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of bacterium total testing pipe, including glass tube 2, the blotting paper 3 being placed in the glass tube 2, the sealing ring 1 that is sealingly disposed in the two ends of the glass tube 2, is adsorbed with nutrient solution, indicator and cold water gel in the blotting paper 3.
The internal diameter of the glass tube 2 is the hollow simple glass pipe of 5-10mm.
The blotting paper 3 is stronger using filter paper, lens wiping paper, fibrous absorbent paper, wood pulp fluff, or other water imbibitions, the paper with certain degree of hardness Yu toughness.
By the resilient sealing ring of tool that is formed in glass cement implantation glass pipe, the glass cement can be sealing neutral silicone resin to the sealing ring 1.
The indicator is 2,3,5- triphenyl tetrazolium chlorides indicator (abbreviation TTC).
The cold water gel is carragheen, Sodium Polyacrylate.
The bacterium total testing pipe production procedure includes step：
1）Prepare nutrient solution, indicator and cold water gel mixed liquor：100mL sterile pure waters are taken, adds peptone, yeast extract, glucose, dipotassium hydrogen phosphate, sodium chloride, TTC to be respectively 2.590g, 1.050g, 0.239g, 0.250g, 0.450g, 0.004g, add carragheen to be respectively 1.000g, 0.300g with Sodium Polyacrylate；
2）The blotting paper of clip suitable dimension, in immersion mixed liquor, after being picked up with tweezers, lies on pallet, it is subsequently placed in baking oven, is dried with the temperature less than 60 DEG C, in order to blotting paper adheres to more mixed liquor compositions, mixed liquor is soaked after can drying again, then is dried, this process is repeated 2~3 times；
3）One section of suitable glass tube of interception, internal diameter is about 5mm, length is about 20cm, during the blotting paper 3 after drying is filled in glass tube 2, water suction, 3 width is just long identical with the inner circumferential of glass tube 2, blotting paper 3 is become a circle, be close on the inner side of glass tube 2, water suction, 3 in the equal retraction 0.5cm in the two ends of glass tube 2, that is, require the length 1cm shorter than glass tube of blotting paper；
4）Sealing neutral silicone resin is injected at the two ends of glass tube 2, thickness is 0.5cm, then stands 24h, allows sealing neutral silicone resin to solidify to form sealing ring 1；
5）The testing tube for preparing is put into baking oven, discontinuous sterilization is carried out, sterilising temp is 70 ± 5 DEG C, and number of times is 3 times, every time after sterilizing, after room temperature places 24 h, then carry out sterilizing next time.
Gone out the testing tube after bacterium, you can for the detection of water sample.
The bacterium total testing pipe water sample detection flow that the present invention is provided is as follows：
Testing process：
1, take 0.5mL or 1mL water samples, pierce through sealing ring 1 with asepsis injector（Because sealing ring has elasticity, can tighten pin hole airtight, make tube environment with the outer isolation of pipe）Slowly in injection testing tube, water sample is allowed to stick on blotting paper 3, concrete water quality can not be excessive, it is impossible in glass, with the presence of the water of flowing in 2, if with the presence of the water of flowing, should reduce concrete water quality.
2, when test tube reclaimed water sample to be measured does not flow, that is, think to be fixed, then testing tube is put into incubator culture, general cultivation temperature is 32 ± 1 DEG C, and the time is 48 ± 3h.
3, after the completion of culture, testing tube is taken out, read the total number of bacteria that the red points in pipe are in water sample.
4, if total number of bacteria is surveyed again than larger after can diluting in water sample.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although being described in detail to the present invention with reference to the foregoing embodiments, it will be understood by those within the art that：It can still modify to the technical scheme described in foregoing embodiments, or carry out equivalent to which part technical characteristic；And these modifications or replacement, do not make the spirit and scope of the essence disengaging various embodiments of the present invention technical scheme of appropriate technical solution.
Claims (9)
1. a kind of bacterium total testing pipe, it is characterised in that：Including glass tube
(2) nutrient solution, indicator and cold water gel are adsorbed with the blotting paper (3), being placed in the glass tube (2), the sealing ring (1) for being sealingly disposed in the glass tube (2) two ends, the blotting paper (3).
2. bacterium total testing pipe according to claim 1, it is characterised in that：The glass tube (2) is with the hollow simple glass pipe that internal diameter is 5-10mm.
3. bacterium total testing pipe according to claim 1, it is characterised in that：The blotting paper (3) is using filter paper, lens wiping paper, fibrous absorbent paper, wood pulp fluff.
4. bacterium total testing pipe according to claim 1, it is characterised in that：By the interior resilient sealing ring of tool for being formed of glass cement implantation glass pipe (2), the glass cement can be sealing neutral silicone resin to the sealing ring (1).
5. bacterium total testing pipe according to claim 1, it is characterised in that：The indicator is 2,3,5- triphenyl tetrazolium chloride indicator.
6. bacterium total testing pipe according to claim 1, it is characterised in that：The cold water gel is carragheen, Sodium Polyacrylate.
7. a kind of preparation method of the bacterium total testing pipe as any one of claim 1 to 6, it is characterised in that including step：
1）Prepare nutrient solution, indicator and cold water gel mixed liquor：Peptone, yeast extract, glucose, dipotassium hydrogen phosphate, sodium chloride, 2,3,5- triphenyl tetrazolium chloride indicator is added to be respectively 2.590g in per 100mL sterile pure waters, 1.050g, 0.239g, 0.250g, 0.450g, 0.004g, adds carragheen to be respectively 1.000g, 0.300g with Sodium Polyacrylate；
2）The blotting paper (3) of clip suitable dimension, in immersion mixed liquor, after being picked up with tweezers, lies on pallet, is subsequently placed in drying in baking oven；
3）One section of suitable glass tube (2) of interception, internal diameter is 5-10mm, length is 20-25cm, during blotting paper (3) after drying is filled in glass tube (2), the width of blotting paper (3) is just long identical with glass tube (2) inner circumferential, blotting paper is set to become a circle, it is close on glass tube (2) inner side, blotting paper (3) require the length of blotting paper (3) than glass tube (2) short 1cm in the equal retraction 0.5cm in glass tube (2) two ends；
4）In glass tube (2) two ends implantation glass glue, thickness is 0.5cm, then stands 20-24 h, allows glass cement to solidify to form sealing ring (1)；
5）The testing tube for preparing is put into baking oven, discontinuous sterilization is carried out, sterilising temp is 70 ± 5 DEG C, and number of times is 3 times, every time after sterilizing, 20-24 is placed in room temperature
After h, then carry out sterilizing next time.
8. preparation method according to claim 7, it is characterised in that：Step 2）Described in baking oven drying temperature be less than 60 DEG C.
9. preparation method according to claim 7, it is characterised in that：Step 2）Described in immersion mixed liquor, after being picked up with tweezers, lie on pallet, be subsequently placed in the process dried in baking oven and repeat 2-3 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511015810.5A CN106932383A (en) | 2015-12-30 | 2015-12-30 | A kind of bacterium total testing pipe and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511015810.5A CN106932383A (en) | 2015-12-30 | 2015-12-30 | A kind of bacterium total testing pipe and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106932383A true CN106932383A (en) | 2017-07-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511015810.5A Pending CN106932383A (en) | 2015-12-30 | 2015-12-30 | A kind of bacterium total testing pipe and preparation method thereof |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB779514A (en) * | 1954-10-04 | 1957-07-24 | Friedrich Josef Forg | Means for carrying out bacteriological tests |
| GB1055387A (en) * | 1963-04-10 | 1967-01-18 | Wilmot Castle Co | Biological sterility indicator and method for using same |
| CN1051758A (en) * | 1990-10-13 | 1991-05-29 | 哈尔滨市卫生防疫站 | Total number of bacterial colony detects glued membrane and preparation method |
| CN1544925A (en) * | 2003-11-14 | 2004-11-10 | 广东工业大学 | Color developing reagent and detector tube for detecting carbon monoxide, fabricating method and application thereof |
| CN2736760Y (en) * | 2004-07-28 | 2005-10-26 | 北京联合大学生物化学工程学院 | Formaldehyde gas detector tube |
| CN101029332A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Bacterium total testing fluid and its testing bottle |
| CN201007706Y (en) * | 2006-11-30 | 2008-01-16 | 白莉 | Colour comparison detecting tube of hydrogen sulfide test |
| CN104611403A (en) * | 2014-12-18 | 2015-05-13 | 湖北工业大学 | Food and tableware coliform bacteria rapid detection scrip preparation and application |
-
2015
- 2015-12-30 CN CN201511015810.5A patent/CN106932383A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB779514A (en) * | 1954-10-04 | 1957-07-24 | Friedrich Josef Forg | Means for carrying out bacteriological tests |
| GB1055387A (en) * | 1963-04-10 | 1967-01-18 | Wilmot Castle Co | Biological sterility indicator and method for using same |
| CN1051758A (en) * | 1990-10-13 | 1991-05-29 | 哈尔滨市卫生防疫站 | Total number of bacterial colony detects glued membrane and preparation method |
| CN1544925A (en) * | 2003-11-14 | 2004-11-10 | 广东工业大学 | Color developing reagent and detector tube for detecting carbon monoxide, fabricating method and application thereof |
| CN2736760Y (en) * | 2004-07-28 | 2005-10-26 | 北京联合大学生物化学工程学院 | Formaldehyde gas detector tube |
| CN101029332A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Bacterium total testing fluid and its testing bottle |
| CN201007706Y (en) * | 2006-11-30 | 2008-01-16 | 白莉 | Colour comparison detecting tube of hydrogen sulfide test |
| CN104611403A (en) * | 2014-12-18 | 2015-05-13 | 湖北工业大学 | Food and tableware coliform bacteria rapid detection scrip preparation and application |
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Application publication date: 20170707 |