CN204945038U - A kind of non-intrusion type biomass on-line measuring device for Shaking culture - Google Patents
A kind of non-intrusion type biomass on-line measuring device for Shaking culture Download PDFInfo
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- CN204945038U CN204945038U CN201520672183.1U CN201520672183U CN204945038U CN 204945038 U CN204945038 U CN 204945038U CN 201520672183 U CN201520672183 U CN 201520672183U CN 204945038 U CN204945038 U CN 204945038U
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Abstract
The utility model discloses a kind of non-intrusion type biomass on-line measuring device for Shaking culture, comprise main control unit, the shaking flask can holding biological suspension and the fixed cover covered on outside shaking flask, fixed cover is connected with detection probe in the bottom outside of shaking flask, detection probe comprise can with the substrate of fitting bottom shaking flask and the light source be located on substrate, photoelectric sensor and acceleration transducer, light source can produce the light path tilting to inject in shaking flask, the scattered light produced after the below that photoelectric sensor is positioned at light path is radiated at biological suspension with optical path, light source, photoelectric sensor and acceleration transducer are all connected with described main control unit.The utility model uses the method for turbidimetry, back scattering optical measurement techniques is adopted to realize the on-line checkingi of Shaking culture bacterial concentration, can when not interrupting culture device, the concentration of suspension biological in shaking flask is detected, its simple structure, cost are low, can effectively improve detection efficiency and precision, avoid sample contamination.
Description
Technical field
The utility model is used for cell or thalline culture technique field, particularly relates to a kind of non-intrusion type biomass on-line measuring device for Shaking culture.
Background technology
Shaking flask because its easily process, cost are low, the feature such as simple is set is widely used in microbial cell and cultivates.In biology laboratory, the monitoring of Shaking culture thalli growth often adopts repeatedly sampling and measuring, draws its optical density (OD), dry cell weight (CDW) or cell concentration.Due to can not the incubation of whole-process automatic monitoring cell, the cultivation of each cell usually all needs to measure off-line value for several times and, until concentration reaches requirement, and then carries out a series of subsequent experimental.For conventional optical density (absorbance) method, along with the continuous increase of turbid liquid concentration, the Spectrophotometric range of linearity (usual OD
600between 0.1-0.7) be not enough to meet the requirement of measuring, need to carry out dilution process to sample.Meanwhile, the shaking flask that shaking table is used or Erlenmeyer flask unspecial sample exit port, and every sub-sampling both increases the risk of bacteria infection.If a laboratory technician monitors the cultivation of several different cell or thalline simultaneously, the measuring process of this repetition will become and waste time and energy, and frequently interrupts the current culture device run.
Utility model content
For solving the problem, the utility model provides a kind of non-intrusion type biomass on-line measuring device for Shaking culture, use the method for turbidimetry, back scattering optical measurement techniques is adopted to realize the on-line checkingi of Shaking culture bacterial concentration, can when not interrupting culture device, detect the concentration of suspension biological in shaking flask, its simple structure, cost are low, can effectively improve detection efficiency and precision, avoid sample contamination.
The utility model solves the technical scheme that its technical matters adopts: a kind of non-intrusion type biomass on-line measuring device for Shaking culture, comprise main control unit, the shaking flask can holding biological suspension and the fixed cover covered on outside described shaking flask, described fixed cover is connected with detection probe in the bottom outside of shaking flask, described detection probe comprises with the substrate of fitting bottom shaking flask and can establish light source on the substrate, photoelectric sensor and acceleration transducer, described light source can produce the light path tilting to inject in shaking flask, described photoelectric sensor is positioned at the below of described light path to measure the scattered light produced after described light path is radiated at biological suspension, described light source, photoelectric sensor and acceleration transducer are all connected with described main control unit.
Be further used as the improvement of technical solutions of the utility model, the PC terminal that described main control unit comprises circuit board, the single-chip microcomputer be connected with described circuit board and is connected with described single-chip microcomputer, described light source, photoelectric sensor are all connected with circuit board by circuit with acceleration transducer.
Be further used as the improvement of technical solutions of the utility model, described substrate is provided with the first jack embedded for described light source and the second jack embedded for photoelectric sensor, described first jack favours at the bottom of shaking flask bottle and arranges, second jack is arranged perpendicular at the bottom of shaking flask bottle, and between described first jack and the second jack, angle is 30 °-60 °.
Be further used as the improvement of technical solutions of the utility model, described substrate is provided with light-blocking block between light source and photoelectric sensor, close contact bottom the top of described light-blocking block and shaking flask.
Be further used as the improvement of technical solutions of the utility model, opening is offered in the side of described fixed cover, and opening is provided with slide fastener, and fixed cover forms the inner conical surface can fitted with described shaking flask outer wall after slide fastener drawing in inside.
Be further used as the improvement of technical solutions of the utility model, described fixed cover above shaking flask bottleneck and/or side be provided with ventilating opening.
Be further used as the improvement of technical solutions of the utility model, described fixed cover is provided with the covering can closing described ventilating opening.
Be further used as the improvement of technical solutions of the utility model, described light source is laser diode, the front end of described laser diode is provided with collimation lens, described photoelectric sensor is the photodiode of two integrated transporting dischargings, two photodiodes are all positioned at the scattered light receiving different distance immediately below light path respectively, and the sensitive surface of two photodiodes is provided with optical filter.
The beneficial effects of the utility model: during utility model works, add the biological suspension of needs cultivation in shaking flask after, start shaking table, main control unit extrapolates the motion state of shaking flask and interior biological suspension thereof by acceleration transducer, i.e. the position of shaking flask at a time residing shaking table movement locus.When shaking flask moves to optimum measurement angle, open light source, main control unit reads scattered light intensity signal measured by photoelectric sensor, change through I/V, the measured value of this measurement is obtained after amplification filtering, the utility model uses the method for turbidimetry, back scattering optical measurement techniques is adopted to realize the on-line checkingi of Shaking culture bacterial concentration, can when not interrupting culture device, the concentration of suspension biological in shaking flask is detected, its simple structure, cost are low, can effectively improve detection efficiency and precision, avoid sample contamination.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described in further detail:
Fig. 1 is the utility model theory structure schematic diagram;
Fig. 2 is A-A place cut-open view in Fig. 1;
Fig. 3 is fixed cover structural representation.
Embodiment
Referring to figs. 1 through Fig. 3, that show the concrete structure of the preferred embodiment of the utility model.The design feature of each element of the utility model will be described in detail below, if and when being described to direction (upper and lower, left and right, before and after), be with the structure shown in Fig. 1 for reference describes, but actual user of the present utility model is to being not limited thereto.
The utility model provides a kind of non-intrusion type biomass on-line measuring device for Shaking culture, comprise main control unit, the shaking flask 1 can holding biological suspension 11 and the fixed cover 2 covered on outside described shaking flask 1, described fixed cover 2 is connected with detection probe 3 in the bottom outside of shaking flask 1, described detection probe 3 comprise can with the substrate 31 of fitting bottom shaking flask 1 and the light source 32 be located on described substrate 31, photoelectric sensor 33 and acceleration transducer 34, acceleration transducer 34 is 3-axis acceleration sensor, when multiple detection probe 3 is run simultaneously, can only detect as motion by detection probe 3 Integrated Accelerometer 34, all the other are not Integrated Accelerometer 34 then, to reduce costs.Described light source 32 can produce the light path tilting to inject in shaking flask 1, light source 32 lighting angle should be little as far as possible, be preferably ± 4 °, described photoelectric sensor 33 is positioned at the below of described light path to measure the scattered light produced after described light path is radiated at biological suspension 11, photoelectric sensor 33 sensitivity and wavelength response range and light source 32 match, and described light source 32, photoelectric sensor 33 are all connected with described main control unit with acceleration transducer 34.The PC terminal 43 that described main control unit comprises circuit board 41, the single-chip microcomputer 42 be connected with described circuit board 41 and is connected with described single-chip microcomputer 42, described circuit board 41 is made up of light source driving circuit, signal processing circuit and power circuit, and described light source 32, photoelectric sensor 33 are all connected with circuit board 41 by circuit with acceleration transducer 34.Described single-chip microcomputer 42 is Arduino single-chip microcomputer, single-chip microcomputer 42 independently can carry out data sampling and processing, preservation and display, also data can be uploaded in real time by serial ports the PC terminal 43 being provided with data analysis and mapping software, store for data and display in real time.
Described substrate 31 is provided with the first jack embedded for described light source 32 and the second jack embedded for photoelectric sensor 33, described first jack favours at the bottom of shaking flask 1 bottle and arranges, second jack is arranged perpendicular at the bottom of shaking flask 1 bottle, between described first jack and the second jack, angle is 30 °-60 °, namely backward 120 ° of-150 ° of scattered lights measured by photoelectric sensor 33, to reduce the impact of liquid level reflection, ambient light noise, improve the measuring accuracy of under extreme measuring condition (the low consumption of the liquid slow-speed of revolution or low consumption of the liquid height rotating speed).In the present embodiment, light source 32 is preferably 45 ° with the angle of photoelectric sensor 33, and photoelectric sensor 33 receives backward 135 ° of scattered lights.
Due to bottom shaking flask 1 with certain radian, therefore the surface of contact of substrate 31 and shaking flask 1 is generally " annulus ", light source 32 is positioned at the inner side of surface of contact " annulus ", and photoelectric sensor 33 is positioned at " annulus " outside, at the transition arc place receiving scattered light of shaking flask 1 bottom surface and the conical surface.Described substrate 31 is provided with light-blocking block 35 between light source 32 and photoelectric sensor 33, and light-blocking block 35 adopts light tight soft material to make, and close contact bottom the top of described light-blocking block 35 and shaking flask 1, avoids light direct irradiation on photoelectric sensor 33.
Described fixed cover 2 can eliminate the impact of surround lighting, fixes shaking flask 1 simultaneously, to ensure stablizing under motion state between shaking flask 1 and detection probe 3.Opening is offered in the side of described fixed cover 2, and opening is provided with slide fastener 21, and fixed cover 2 forms the inner conical surface can fitted with described shaking flask 1 outer wall after slide fastener 21 drawing in inside.Described fixed cover 2 above shaking flask 1 bottleneck and/or side be provided with ventilating opening 22.Described fixed cover 2 is provided with the covering can closing described ventilating opening 22, directly enters shaking flask 1 by top portion ventilation mouth 22 to prevent foreign material and liquid.
Described light source 32 is laser diode, the front end of described laser diode is provided with collimation lens, and temperature compensation module is installed, described photoelectric sensor 33 is the photodiode of two integrated transporting dischargings, two photodiodes are all positioned at the scattered light receiving different distance immediately below light path respectively, and the sensitive surface of two photodiodes is provided with optical filter.
The principle of a kind of non-intrusion type biomass on-line measuring device for Shaking culture that the utility model provides is as follows:
First, the detection probe 3 of this device is fixed on shaking table.Before shaking table starts, static shift correction and compensation are carried out to acceleration transducer 34, and by offset data stored in single-chip microcomputer 42.Add the biological suspension 11 of needs cultivation in shaking flask 1 after, place it in fixed cover 2, tension slide fastener 21 is also fixing.Start shaking table, shaking table movement locus is circular, single-chip microcomputer 42 is by reading acceleration transducer 34 fast in horizontal X, the vector value of Y both direction acceleration also extrapolates the motion state of shaking flask 1 and interior biological suspension 11 thereof by anti-triangle or arctan function, i.e. the position of shaking flask 1 at a time residing shaking table movement locus.When shaking flask 1 moves to optimum measurement angle, open light source 32, single-chip microcomputer 42 reads scattered light intensity signal measured by photoelectric sensor 33 fast by its AD conversion module, change through current/voltage (I/V), the measured value of this measurement is obtained after amplification filtering, and this measured value is preserved and display, by serial communication, measurement data is uploaded to PC terminal 43 simultaneously and carry out drawing and showing.Along with constantly carrying out of cultivating, the concentration of biological suspension 11 becomes large gradually, and the scattered light signal measured by photoelectric sensor 33 is constantly strengthened, and just can be extrapolated the concentration of liquid in current bottle by the measurement correction model of the biological suspension of correspondence 11.
The present embodiment, using off-line optical density (OD) value as the control reference of on-line measurement value, sets up different correction models to corresponding bacterium liquid.The value of the absorbance of the described off-line optical density liquid that to be spectrophotometer record at specific wavelength.Meanwhile, moving average filtering process can be adopted to measured value, with level and smooth experiment curv, reduce measurement noises.
Certainly, the invention is not limited to above-mentioned embodiment, those of ordinary skill in the art also can make equivalent variations or replacement under the prerequisite without prejudice to the utility model spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (8)
1. the non-intrusion type biomass on-line measuring device for Shaking culture, it is characterized in that: comprise main control unit, the shaking flask can holding biological suspension and the fixed cover covered on outside described shaking flask, described fixed cover is connected with detection probe in the bottom outside of shaking flask, described detection probe comprises with the substrate of fitting bottom shaking flask and can establish light source on the substrate, photoelectric sensor and acceleration transducer, described light source can produce the light path tilting to inject in shaking flask, described photoelectric sensor is positioned at the below of described light path to measure the scattered light produced after described light path is radiated at biological suspension, described light source, photoelectric sensor and acceleration transducer are all connected with described main control unit.
2. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 1, it is characterized in that: the PC terminal that described main control unit comprises circuit board, the single-chip microcomputer be connected with described circuit board and is connected with described single-chip microcomputer, described light source, photoelectric sensor are all connected with circuit board by circuit with acceleration transducer.
3. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 1, it is characterized in that: described substrate is provided with the first jack embedded for described light source and the second jack embedded for photoelectric sensor, described first jack favours at the bottom of shaking flask bottle and arranges, second jack is arranged perpendicular at the bottom of shaking flask bottle, and between described first jack and the second jack, angle is 30 °-60 °.
4. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 1, is characterized in that: described substrate is provided with light-blocking block between light source and photoelectric sensor, close contact bottom the top of described light-blocking block and shaking flask.
5. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 1, it is characterized in that: opening is offered in the side of described fixed cover, opening is provided with slide fastener, and fixed cover forms the inner conical surface can fitted with described shaking flask outer wall after slide fastener drawing in inside.
6. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 5, is characterized in that: described fixed cover above shaking flask bottleneck and/or side be provided with ventilating opening.
7. the non-intrusion type biomass on-line measuring device for Shaking culture according to claim 6, is characterized in that: described fixed cover is provided with the covering can closing described ventilating opening.
8. the non-intrusion type biomass on-line measuring device for Shaking culture according to any one of claim 1-7, it is characterized in that: described light source is laser diode, the front end of described laser diode is provided with collimation lens, described photoelectric sensor is the photodiode of two integrated transporting dischargings, two photodiodes are all positioned at the scattered light receiving different distance immediately below light path respectively, and the sensitive surface of two photodiodes is provided with optical filter.
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| CN201520672183.1U CN204945038U (en) | 2015-08-31 | 2015-08-31 | A kind of non-intrusion type biomass on-line measuring device for Shaking culture |
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| CN201520672183.1U CN204945038U (en) | 2015-08-31 | 2015-08-31 | A kind of non-intrusion type biomass on-line measuring device for Shaking culture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044038A (en) * | 2015-08-31 | 2015-11-11 | 广州中国科学院先进技术研究所 | Non-invasive biomass online detection device used for shake-flask culture |
| CN108064338A (en) * | 2017-11-06 | 2018-05-22 | 广州中国科学院先进技术研究所 | A kind of immersion type biomass on-line measuring device |
-
2015
- 2015-08-31 CN CN201520672183.1U patent/CN204945038U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044038A (en) * | 2015-08-31 | 2015-11-11 | 广州中国科学院先进技术研究所 | Non-invasive biomass online detection device used for shake-flask culture |
| CN108064338A (en) * | 2017-11-06 | 2018-05-22 | 广州中国科学院先进技术研究所 | A kind of immersion type biomass on-line measuring device |
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