CN102519898A - Device utilizing single light source to detect fermentation liquid of shake table - Google Patents
Device utilizing single light source to detect fermentation liquid of shake table Download PDFInfo
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- CN102519898A CN102519898A CN2011104491600A CN201110449160A CN102519898A CN 102519898 A CN102519898 A CN 102519898A CN 2011104491600 A CN2011104491600 A CN 2011104491600A CN 201110449160 A CN201110449160 A CN 201110449160A CN 102519898 A CN102519898 A CN 102519898A
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Abstract
The invention provides a device utilizing a single light source to detect the fermentation liquid of a shake table, which comprises shake table equipment, and a triangular flask which is arranged on the shake table for liquid fermentation, wherein the triangular flask is the glass triangular flask which contains the fermentation liquid and is coated with a black coating, and is fixedly arranged on a silicon frame, the silicon frame is fixedly arranged on a shake table tray, and the residual parts of the triangular flask is coated with the shading black coating expect the bottom and light holes which correspond to two parallel positions; and a light emission electrode and a light receiving electrode which correspond to each other are respectively and fixedly arranged at the two sides of the silicon frame, and are matched with the light holes of the triangular flask which contains the fermentation liquid at the middle part, the light of the light emission electrode at one end penetrates through the fermentation liquid to be received by the light receiving electrode at the other end, so the light signal is changed into the electrical signal, the concentration of the fermentation liquid is increased along with the gradual increasing of the biological bacteria number in the triangular flask, so the transmitted light is changed, the electrical signal valve which is received by the light receiving electrode is also changed consequently, and accordingly, the growing degree of the bacteria of the fermentation liquid in the triangular flask can be determined. The invention has the advantage that the test device is simple and accurate.
Description
Technical field
The present invention relates to biological technical field.
Background technology
Early stage shaking table major function is that the expanding propagation bacterial classification is used for producing, and needs with production, and shaking table has increased accurate revolution and thermostatic control device, but the Based Intelligent Control that still is unrealized; In recent years the someone proposes in each triangular flask on shaking table to increase in automatic extra-nutrition liquid and the adjustment fermentation liquor functions such as ph, gives the function that triangular flask is similar to conventional fermentation tank, and purpose is to create best yeasting, obtains best ferment effect; But this will make the triangular flask interface very complicated, will bring great difficulty for this equipment of suitability for industrialized production.Detect the fermented liquid quality at present, still with the thalline counting method, i.e. sampling and measuring fermented liquid bacterial content, or filtration takes by weighing filter residue and calculate wet thallus weight, or take by weighing dry weight after the oven dry, or chemical composition analysis etc. is carried out in sampling; Be referred to as online contact and detect, its operating process is comparatively complicated, and error is also very big.
Summary of the invention
The present invention is the basis with the colloid photoelectricity principle of dindar, and thalline can produce macromolecular glucide and make the solution colloidization at the growth certain phase, particularly increases with thalline, and the liquid absorbance also increases thereupon.
Scheme of the present invention is; Comprise shaking table equipment, place the triangular flask that carries out liquid fermentation on the shaking table, it is characterized in that: fill fermented liquid and scribble the glass triangle bottle of black coating; Be fixed on the silica gel frame; The silica gel frame is fixed on the shaking table pallet, and triangular flask is except that the light hole of bottom and two horizontal level correspondences, and all the other all scribble the shading black coating; Be fixed with corresponding light emitting electrode and light-receiving electrode in the both sides of silica gel frame; And match with triangular flask light hole position that fermented liquid is equipped with in the centre, the light of an end emitting electrode sees through fermented liquid and is received by other end light-receiving electrode; Make light signal become electric signal; Along with the biological bacteria scale of construction in the triangular flask increases gradually, fermented liquid concentration increases, and the light that sees through is changed; The electric signal numerical value that the light-receiving electrode receives also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in the triangular flask.
Said light-receiving electrode one end is connected with photoelectric sensor, causes the attenuation change of light to cause change in electric owing to fermented liquid concentration changes; Through being wirelessly transmitted to display screen; Or mobile phone, or on the media player, to show the extent of growth of fermentation liquor thalline in the shaking table triangular flask.
Said transmitting illuminant adopts optical filter or grating, and making light source is the ultraviolet light of infrared light or selected wavelength.
Said light hole is positioned at below the liquid level of fermented liquid, corresponding to the horizontal level of triangular flask outer wall both sides.
Utilize the photoelectric absorption principle; Adopt special shading flask; Need not take a sample to obtain the various information of fermented liquid growth conditions, realizes the online non-contact detection of fermented liquid, and can be depicted as standard working curve figure; The best growing condition that is used for accurately studying the thalline fermentation for shaking table brings technological breakthrough, brings great convenience for accuracy instructs industrial fermentation.
Advantage of the present invention is: the digital signal that 1) adopts opto-electronic conversion is as detection means, and the light source propagation distance is far away, and can reach tens of km p.s., and very-short-reach propagates that wherein error is minimum in triangular flask.2) thalline increases with light absorption value and increases to direct ratio, with photodetection liquid absorbance accurately and fast, easy to operate.3) utilize optical filter to obtain light source, easy to be durable, technology maturation is easy to realize suitability for industrialized production.4) compare with the traditional glass Erlenmeyer flask, adopt coating shading triangular flask, many advantages are arranged: at first the black coating light-proofness is good, can avoid diffracted ray to disturb, and guarantees the photoelectricity operating accuracy; Secondly, with the shading of chrome-plated material coating, the triangular flask conduction is good, and the temperature that can effectively utilize shaking table to provide also provides advantage for exploitation bottom alternating temperature shaking table pallet; 5) the silica stationary frame is flexible, and is sturdy and durable, not only prevents the triangular flask displacement and comes off, can also the in-built electrical device.6) single light source of the present invention detects the device of shaker fermentation liquid, can draw the photoelectric absorption curve through revision test, according to curve programming Based Intelligent Control shaking table, repeats to ferment each stage, operates production routine accurately for industrial bulk fermentation provides.7) need not take a sample to detect and to obtain fermentation condition and relevant information, the design of shaker fermentation orthogonal experiment particularly can be provided, accurate experiment parameter is provided, improve scientific research efficient.
Description of drawings:
Fig. 1 detects shaking table, fixed mount and triangular flask structural drawing for single light source of the present invention;
The single triangular flask structural drawing of Fig. 2.
Code description among the figure:
1, triangle shakes bottle; 2, photosensitive area;
3, silica gel frame; 4, light emitting electrode;
5, light absorption electrode; 6, shaking table pallet;
7, set bolt; 8, photoelectric conversion sensor;
9, emission light light hole; 10, absorbing light light hole; 11, shading coating.
Embodiment:
Consult shown in the accompanying drawing; Fill the triangular flask 1 of culture bacteria liquid fermented liquid, be fixed on the silica gel frame 3, the silica gel frame is fixed on the shaking table pallet 6 by set bolt 7; Be fixed with in correspondence with each other light emitting electrode 4 and light-receiving electrode 5 in the both sides of silica gel frame 3, the middle part is used to place triangular flask 1; Triangular flask 1 is except that the light hole 9,10 of at the bottom of the bottle and two location, and all the other all scribble black shading coating 11; Be positioned at below the fermentation liquid level, said smooth emitting electrode 4 and light absorption electrode 5 are arranged, and match with light hole 9,10; When a side of triangular flask, the light that the light emitting electrode sends sees through fermentation liquor and is received by triangular flask opposite side light absorption electrode; Become digital signal through photoelectric conversion sensor 8, increase with thalline content, fermented liquid concentration changes; The light that sees through liquid also can change, thus the extent of growth of bacterium liquid in the true triangular flask.
Said light-receiving electrode one end; Because fermented liquid concentration changes attenuation change and the change in electric cause light, through be wirelessly transmitted to display screen and (or) mobile phone; (or) on the media player, to show the extent of growth of fermentation liquor thalline in the shaking table triangular flask.
Said transmitting illuminant adopts optical filter or grating, and making light source is the ultraviolet light of infrared light or selected wavelength.
Said except that triangular flask except that bottom and light hole, all be painted with the black of shading; Light hole is positioned at below the liquid level of fermented liquid, and level is corresponding to the both sides of triangular flask outer wall.
Prolong with the thalline fermented incubation time, bacterial concentration increases, and thalline content increases, and is directly proportional with the absorbance increase; Through observing the variation of absorbance, can confirm the product quality of shaker fermentation liquid.The biological shaking table triangular flask of photoelectricity removes the employing shading and films and leave the sensitization position, and other and common Erlenmeyer flask are identical; Single light source detects fermentation shaking table workflow: 1) Ensure Liquid fermentation liquor in triangular flask, according to the different strain growth needs, accurately prepare nutrient matrix; When using 250ml shading triangular flask, add nutrient liquid 100ml or 120ml, loading amount error＜± 0.1%; 2) fermented liquid sterilization, with the shading triangular flask that is added with fermented liquid, through high pressure steam sterilization, in 121 ℃, pressure 0.15Mpa kept 15 minutes, can insert bacterial classification when being cooled to 23 ~ 26 ℃; 3) inoculation, when inoculating spherical liquid spawn, bacterium ball concentration is controlled at 10 in the bacterium liquid
6 ~ 7Individual/ml, every bottle adds 5 ~ 10ml bacterium liquid; When inoculating thread thalline, use 0.5cm
2Card punch is got 5 ~ 10 of mycoderms, inserts to contain in the shading triangular flask of nutrient liquid; During inoculation wherein at least one triangular flask do not add bacterial classification, be contrast; 4) open lighting electrode adjustment electroluminescent lamp; The light source that lamp source is sent sees through triangular flask photosensitive area position and gets into nutrient liquid; Received by the opposite side light absorption electrode of triangular flask, change electric signal into, handle obtaining tangible numerical signal again through the multiplication amplifying circuit.
For satisfying L
9(3)
4Orthogonal test needs, and is one group with 10 triangular flasks usually, and wherein 9 are inserted bacterial classifications, and 1 does not connect bacterial classification as contrast, and triplicate is averaged and done regretional analysis.The silica stationary frame is anchored on the shaking table pallet 6, and lighting electrode, absorbing light electrode and cable place in the silica gel frame, draws outside the machine with the grooved socket at last.
Just timing is through the fine setting turn-knob, photoelectricity received the display number put all and set to zero, and starts shaking table after setting revolution (150 ~ 160 rev/mins), temperature (25 ~ 28 ℃); With shaking table work time lengthening; Changing appears in numerical value on the photoelectricity receptor, when numerical value is constant till, at this moment numerical value is big; Thalline content is maximum in the corresponding triangular flask, and control bottle numerical value still is zero.
Embodiment 1:
Bacterial classification: get three kinds of different barmses, BO21, BO22 and BO23, processing bacteria containing amount respectively is 10
5 ~ 6Individual/ml suspension bacteria liquid is subsequent use;
Sterilization: get 250ml shading triangular flask, respectively add PDA potato nutrient solution 100ml＜± 0.1% after the numbering, sterilization cooling back with pipettor to wherein three each accurately add reference numeral bacterial classification 5ml; More than every bottle of triplicate; Only one does not add bacterium liquid, is used for contrast, totally 10 bottles;
Install: the shading triangular flask is fixed on the silica gel frame, and the turn-knob of screwing, optoelectronic pole and triangular flask photosensitive region are matched.Open photovoltaic power source on the control panel, make the lighting electrode of silica gel frame one side send specific light wave, see through triangular flask photosensitive area position through optical filter, to the light absorption electrode of silica gel frame opposite side,
The start operation: open shaking table control panel photoelectricity master switch, through fine setting, make all triangular flask photoelectricity receptor absorbances be zero, adjustment shaking table temperature is 26 ℃, and revolution is 160 rev/mins, starts shaking table;
Observe: every measured an absorbance value at a distance from three hours, up to absorbance value decline appears and when constant till.With time is horizontal ordinate, is ordinate with the absorbance value, and curve plotting is the best fermentation time of this mushroom type with the light absorption peak value when the highest; With light absorption peak value the maximum, be Cui dominant strain.
Embodiment 2:
Get the advantage yeast strain among the embodiment 1, processing bacteria containing amount is 10
5 ~ 6Individual/ml suspension bacteria liquid is subsequent use; Sterilization, installation and start operation are with embodiment 1; Whenever at a distance from absorbance of detection in 3 hours, sample analysis thalline content, alcohol content,, suitably add nutrient solution and adjustment ph after the work of photoelectric intelligent shaking table according to analysis result; Data storage; Repeated experiments is three times continuously, average, and with time horizontal ordinate; With absorbance, thalline content and alcohol content is that ordinate is figure, draws this bacterial strain photoelectric intelligent and detects the shaking table working curve.Its method of application is: the nutrient formulation that provides by this shaking table; Bottle, sterilization, cooling inoculation; Installation, start etc., the corresponding result of absorbance value and typical curve absorbance value in the close observation fermentation time, close in value shows that fermentation system is in proper working order; And press typical curve prompting suitably adding nutrient solution and adjustment ph, until end; If numerical value differs greatly, have unusual that warning and prompting possibly occur, shutdown inspection is avoided loss in case of necessity.
Claims (4)
1. a single light source detects the device of shaker fermentation liquid; Comprise shaking table equipment, place the triangular flask that carries out liquid fermentation on the shaking table, it is characterized in that: fill fermented liquid and scribble the glass triangle bottle of black coating; Be fixed on the silica gel frame; The silica gel frame is fixed on the shaking table pallet, and triangular flask is except that the light hole of bottom and two horizontal level correspondences, and all the other all scribble the shading black coating; Be fixed with corresponding light emitting electrode and light-receiving electrode in the both sides of silica gel frame; And match with triangular flask light hole position that fermented liquid is equipped with in the centre, the light of an end emitting electrode sees through fermented liquid and is received by other end light-receiving electrode; Make light signal become electric signal; Along with the biological bacteria scale of construction in the triangular flask increases gradually, fermented liquid concentration increases, and the light that sees through is changed; The electric signal numerical value that the light-receiving electrode receives also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in the triangular flask.
2. single light source according to claim 1 detects the device of shaker fermentation liquid, it is characterized in that said light-receiving electrode one end; Be connected with photoelectric sensor; Owing to changing, fermented liquid concentration cause the attenuation change of light to cause change in electric, through being wirelessly transmitted to display screen, or mobile phone; Or on the media player, to show the extent of growth of fermentation liquor thalline in the shaking table triangular flask.
3. single light source according to claim 1 detects the device of shaker fermentation liquid, it is characterized in that, said transmitting illuminant adopts optical filter or grating, and making light source is the ultraviolet light of infrared light or selected wavelength.
4. single light source according to claim 1 detects the device of shaker fermentation liquid, it is characterized in that said light hole is positioned at below the liquid level of fermented liquid, corresponding to the horizontal level of triangular flask outer wall both sides.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102539366A (en) * | 2011-12-29 | 2012-07-04 | 上海智城分析仪器制造有限公司 | Device for detecting swinging bed fermentation liquid by double light sources |
| CN103323433A (en) * | 2013-06-20 | 2013-09-25 | 上海智城分析仪器制造有限公司 | Shaking bed device of non-contact-type multichannel online detection fermentation liquid as well as testing method |
| CN104007054A (en) * | 2014-06-13 | 2014-08-27 | 南京工业大学 | Device and method for detecting cell concentration on line in biological fermentation process |
| CN105907636A (en) * | 2016-05-20 | 2016-08-31 | 长沙湘资生物科技有限公司 | Automatic instrument for microorganism high-flux culture and large concentration range real-time detection |
| CN108064338A (en) * | 2017-11-06 | 2018-05-22 | 广州中国科学院先进技术研究所 | A kind of immersion type biomass on-line measuring device |
| CN108686732A (en) * | 2018-07-27 | 2018-10-23 | 山东大学齐鲁医院 | A kind of rotatable test tube holder |
| CN109385363A (en) * | 2017-08-14 | 2019-02-26 | 宁波方太厨具有限公司 | A kind of installation for fermenting |
| CN109486650A (en) * | 2018-09-20 | 2019-03-19 | 宁波新芝生物科技股份有限公司 | A kind of microbial growth curve analyzer |
| CN112557323A (en) * | 2020-12-04 | 2021-03-26 | 殷国松 | Spectrophotometer and gas detection method |
| CN114235685A (en) * | 2021-11-03 | 2022-03-25 | 广州先进技术研究所 | Shake flask cultured biomass on-line measuring device |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539366B (en) * | 2011-12-29 | 2013-12-18 | 上海智城分析仪器制造有限公司 | Device for detecting swinging bed fermentation liquid by double light sources |
| CN102539366A (en) * | 2011-12-29 | 2012-07-04 | 上海智城分析仪器制造有限公司 | Device for detecting swinging bed fermentation liquid by double light sources |
| CN103323433A (en) * | 2013-06-20 | 2013-09-25 | 上海智城分析仪器制造有限公司 | Shaking bed device of non-contact-type multichannel online detection fermentation liquid as well as testing method |
| CN103323433B (en) * | 2013-06-20 | 2016-08-10 | 上海智城分析仪器制造有限公司 | The shaking table device of contactless multichannel on-line checking fermentation liquid and method of testing |
| CN104007054A (en) * | 2014-06-13 | 2014-08-27 | 南京工业大学 | Device and method for detecting cell concentration on line in biological fermentation process |
| CN105907636A (en) * | 2016-05-20 | 2016-08-31 | 长沙湘资生物科技有限公司 | Automatic instrument for microorganism high-flux culture and large concentration range real-time detection |
| CN105907636B (en) * | 2016-05-20 | 2017-02-22 | 杰灵仪器制造（天津）有限公司 | Automatic instrument for microorganism high-flux culture and large concentration range real-time detection |
| CN109385363B (en) * | 2017-08-14 | 2024-01-16 | 宁波方太厨具有限公司 | Fermentation device |
| CN109385363A (en) * | 2017-08-14 | 2019-02-26 | 宁波方太厨具有限公司 | A kind of installation for fermenting |
| CN108064338A (en) * | 2017-11-06 | 2018-05-22 | 广州中国科学院先进技术研究所 | A kind of immersion type biomass on-line measuring device |
| CN108686732A (en) * | 2018-07-27 | 2018-10-23 | 山东大学齐鲁医院 | A kind of rotatable test tube holder |
| CN109486650B (en) * | 2018-09-20 | 2022-03-25 | 宁波新芝生物科技股份有限公司 | Microorganism growth curve tester |
| CN109486650A (en) * | 2018-09-20 | 2019-03-19 | 宁波新芝生物科技股份有限公司 | A kind of microbial growth curve analyzer |
| CN112557323A (en) * | 2020-12-04 | 2021-03-26 | 殷国松 | Spectrophotometer and gas detection method |
| CN114235685A (en) * | 2021-11-03 | 2022-03-25 | 广州先进技术研究所 | Shake flask cultured biomass on-line measuring device |
| CN114235685B (en) * | 2021-11-03 | 2023-08-08 | 广州先进技术研究所 | On-line biomass detection device for shake flask culture |
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