CN103981089A - Solar microalgae biological reaction system - Google Patents

Solar microalgae biological reaction system Download PDF

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Publication number
CN103981089A
CN103981089A CN201410251520.XA CN201410251520A CN103981089A CN 103981089 A CN103981089 A CN 103981089A CN 201410251520 A CN201410251520 A CN 201410251520A CN 103981089 A CN103981089 A CN 103981089A
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micro
reaction system
reactor body
sun power
solar
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李纪顺
魏艳丽
任艳
杨合同
张新建
孟庆军
李红梅
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BIOTECHNOLOGY CENTER OF SHANDONG ACADEMY OF SCIENCES
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    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
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    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C12M41/08Means for changing the orientation
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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    • C12M43/00Combinations of bioreactors or fermenters with other apparatus
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    • C12M43/00Combinations of bioreactors or fermenters with other apparatus
    • C12M43/08Bioreactors or fermenters combined with devices or plants for production of electricity

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Abstract

The invention discloses a solar microalgae biological reaction system which comprises a reactor main body, a solar collector, a solar cell illuminating system, a carbon dioxide supply system and a control device. The invention provides a high-density culture system with controllable temperature, pH and rotation speed; and the system uses sunlight as a daytime light source and a solar LED (light-emitting diode) lamp as a nighttime light source, and implements carbon dioxide supply, detection and automatic control. The reactor enhances the photic area of the microalgae culture solution per unit volume in the form of an internal light source, and can obviously enhance the yield of the microalgae.

Description

The micro-algae bio-reaction system of a kind of sun power
Technical field
The present invention relates to the biological technical field that micro-algae is cultivated, especially a kind of sun power that adopts is cultivated optical-biological reaction system as micro-algae of light source.
Background technology
Biofuel is a kind of desirable renewable energy source, and the current positive Devoting Major Efforts To Developing biofuel of China also advances its industrialization process.Certain slightly algae because of its have oleaginousness high, be easy to cultivate, the feature such as yield per unit is large, be the biodiesel raw material of high-quality.Although micro-algae biofuel has huge advantage, utilize at present micro-algae production biofuel to be scarcely out of swaddling-clothes, its Main Bottleneck is just high cultivation cost, and the microalgae culture system of therefore developing a low-cost high-efficiency is to realize a key step utilizing micro-algae production biofuel.
Micro-algae training method mainly contains open and closed two kinds, and it is simple, with low cost and easy and simple to handle that Race-way photobioreactor builds, but has the shortcomings such as vulnerable to pollution, culture condition be unstable.Closed reactor culture condition is stable, can aseptic technique, easily carry out high-density culture, and become developing direction from now on.Closed photo bioreactor has duct type, flat, column air lift type, stirred-tank fermenter, floating bag film etc.At present, the daylighting way of closed photo bioreactor has two kinds of outdoor direct daylighting and artificial light daylightings.Because operation and the construction cost of source of artificial light in bioreactor is the maximum bottleneck that micro algae growth meets with, therefore, how to reduce light source cost, and it is particularly important to promote light utilising efficiency.In all light sources, sun power is one of the abundantest natural resource of the earth, uses sun power not only there is no the problem of source of artificial light power consumption, and has broad optical wavelength, is applicable to very much the cultivation of micro-algae.
Light energy conversion is the important factor that affects micro algae growth, and the selection of light source, installation directly affect reactor light distribution, have influence on frustule absorbing light.
Summary of the invention
Object of the present invention is exactly to provide a kind of sun power micro-algae bio-reaction system for the defect of above-mentioned existence.The micro-algae bio-reaction system of sun power of the present invention comprises reactor body, solar collector, solar cell lighting system, carbonic acid gas plenum system, control device, adopt sunlight as light source on daytime, solar LED (Light Emitting Diode) lamp is as night light source, carbonic acid gas supply, detection and automatization are controlled, and temperature, pH, the controlled high-density culture system of rotating speed.This reactor has improved the light-receiving area of unit volume micro algae culturing liquid with inner light source form, can obviously improve micro-algae output.
The micro-algae bio-reaction system of a kind of sun power of the present invention technical scheme is that this system comprises reactor body, solar collector, solar cell lighting system, carbonic acid gas plenum system, control device; Wherein, reactor body arranged outside has photoconductive fiber, and photoconductive fiber is connected with solar collector; Control device is controlled respectively the work of solar illuminating system and carbonic acid gas plenum system; In reactor body, be provided with temperature sensor, pH sensor, CO 2/ O 2sensor, temperature sensor, pH sensor, CO 2/ O 2sensor is connected with control device respectively.
Reactor body is made with the pmma material of heat-resistant pressure-resistant.
Solar cell lighting system comprises solar panel, store battery, LED auxiliary lighting apparatus, LED light source.This system daytime is electric energy by transform light energy, converts electrical energy into luminous energy night, and utilizes fiber optic conduction system to offer culture systems.
Solar collector is comprised of collecting unit, and each collecting unit comprises condenser lens and photoconductive fiber.Photoconductive fiber adopts the superbright entirely lighting plastics multimode optical fibers 20-100 root of 1-5mm, and one end connects solar collector, and one end is centered around reactor body and carries out light filling around.
Each 30 of the superbright entirely lighting plastics multimode optical fiberss of preferred photoconductive fiber employing 2mm and 3mm, are used in combination, and one end connects solar collector, and one end is centered around reactor body and carries out light filling around.
Carbonic acid gas plenum system comprises carbon dioxide generating device, peristaltic pump, gas transmission pipeline, liquid transmission line.Control device adds by detecting culture tank gas concentration lwevel control peristaltic pump stream, and the carbonic acid gas of generation is by the direct supplied reactor main body of pipeline, for micro-algae photosynthesis is supplied raw materials.
Reactor body below is also provided with heating jacket.
Reactor body is also connected with chilled water tank, and control device is controlled the cooling water flow of chilled water tank by the temperature in monitoring reactor body.
The controller that control device of the present invention adopts can be PLC controller.Control device is processed the temperature, pH, carbonic acid gas and the oxygen signal that receive, and by communication network, data are sent to computer, the fermentation control platform being installed on computer is processed online detection data analysis, according to temperature, pH, carbonic acid gas and the oxygen parameter set up in advance (different parameters can be set according to cultivating algae difference), to control device, send order, comprise and start or stop peristaltic pump, the stream rate of acceleration of peristaltic pump.
Beneficial effect of the present invention is: the micro-algae bio-reaction system of sun power of the present invention comprises reactor body, solar collector, solar cell lighting system, carbonic acid gas plenum system, control device, adopt sunlight as light source on daytime, solar LED (Light Emitting Diode) lamp is as night light source, carbonic acid gas supply, detection and automatization are controlled, and temperature, pH, the controlled high-density culture system of rotating speed.This reactor has improved the light-receiving area of unit volume micro algae culturing liquid with inner light source form, can obviously improve micro-algae output.
accompanying drawing explanation:
Figure 1 shows that the structural representation of the micro-algae bio-reaction system of sun power of the present invention.
Wherein, 1-solar collector, 2-light source, 3-solar panel, 4-photoconductive fiber, 5-temperature sensor, 6-pH sensor, 7-CO 2/ O 2sensor, 8-control device, 9-chilled water tank, 10-reactor body, 11-heating jacket, 12-carbonic acid gas plenum system.
embodiment:
In order to understand better the present invention, with specific examples, describe technical scheme of the present invention in detail below, but the present invention is not limited thereto.
Embodiment 1
The micro-algae bio-reaction system of a kind of sun power of the present invention comprises reactor body 10, solar collector 1, solar cell lighting system, carbonic acid gas plenum system 12, control device 8; Wherein, reactor body 10 arranged outside have photoconductive fiber 4, and photoconductive fiber 4 is connected with solar collector 1; Control device 8 is controlled respectively the work of solar illuminating system and carbonic acid gas plenum system 12; In reactor body 10, be provided with temperature sensor 5, pH sensor 6, CO 2/ O 2sensor 7, temperature sensor 5, pH sensor 6, CO 2/ O 2sensor 7 is connected with control device 8 respectively.
Reactor body 10 is made with the pmma material of heat-resistant pressure-resistant.
Solar cell lighting system comprises solar panel 3, store battery, LED auxiliary lighting apparatus, LED light source 2.This system daytime is electric energy by transform light energy, converts electrical energy into luminous energy night, and utilizes fiber optic conduction system to offer culture systems.
Solar collector 1 is comprised of collecting unit, and each collecting unit comprises condenser lens, photoconductive fiber 4, has guaranteed optical throughput, for micro-algae provides enough rayed.Each 30 of the superbright entirely lighting plastics multimode optical fiberss of photoconductive fiber 4 employing 2mm and 3mm, are used in combination, and one end connects solar collector 1, and one end is centered around reactor body 10 and carries out light filling around.
Carbonic acid gas plenum system 12 comprises carbon dioxide generating device, peristaltic pump, gas transmission pipeline, liquid transmission line.Control device 8 adds by detecting culture tank gas concentration lwevel control peristaltic pump stream, and the carbonic acid gas of generation is by the direct supplied reactor main body 10 of pipeline, for micro-algae photosynthesis is supplied raw materials.
Reactor body 10 belows are also provided with heating jacket 11.
Reactor body 10 is also connected with chilled water tank 9, and control device 8 is controlled the cooling water flow of chilled water tank 9 by the temperature in monitoring reactor body 10.
The controller that control device 8 of the present invention adopts can be PLC controller.The temperature of 8 pairs of receptions of control device, pH, carbonic acid gas and oxygen signal are processed, and by communication network, data are sent to computer, the fermentation control platform being installed on computer is processed online detection data analysis, according to temperature, pH, carbonic acid gas and the oxygen parameter set up in advance (different parameters can be set according to cultivating algae difference), to control device, send order, comprise and start or stop peristaltic pump, the stream rate of acceleration of peristaltic pump.
Embodiment 2
Nitzschia closterium minutissima inoculum density is 2.8 * 10 5cell/mL, culture temperature is 20 ℃, intensity of illumination is 54 μ E/ (m 2s), continuous illumination autotrophy is cultivated 6d to logarithmic phase, collects frond, and secondary inoculation is in reactor body 10, and inoculum density is 10 * 10 5, to cultivate, intensity of illumination is 54 μ E/ (m 2s), provide 15% CO 2gas and air gas mixture pass in reactor body 10 continuously, and air flow is 300mlmin -1, rotating speed is 120rpm, keeps pH value between 7-8,20d is cultivated in continuous illumination, utilizes tail-gas analyzer to record CO in reactor body 10 2content, and regularly sampling, counting.
Result shows: utilize optical-biological reaction system of the present invention to cultivate Nitzschia closterium minutissima, by detecting CO in tail gas 2concentration studies the growth rhythm of frustule, the maximum amount of gathering is 42.0 * 10 11cell, adopts flat bioreactor to cultivate 3.12 * 10 of Nitzschia closterium minutissima acquisition apparently higher than (2009) such as Sun Liqin 12biomass.
Embodiment 3
Select pollution-free, well-grown chlamydomonas kind, utilize illumination reaction device of the present invention to carry out expanding species cultivation, the substratum of employing is SE substratum.The algae liquid inoculation of getting exponential phase of growth, inoculum density is 2.56 * 10 6cell/mL, culture temperature is 28 ℃, intensity of illumination is 108 μ E/ (m 2s), provide 15%CO 2gas and air gas mixture pass in reactor body 10 continuously, and air flow is 300mlmin -1, rotating speed is 120rpm, keeps pH value between 7-8,14d is cultivated in continuous illumination, utilizes tail-gas analyzer to record CO in reactor body 10 2content, and regularly sampling, counting.
Result shows, uses bioreactor of the present invention, and chlamydomonas is cultivated its biomass after 14 days can reach 3.2g/L.

Claims (9)

1. the micro-algae bio-reaction system of sun power, is characterized in that, this system comprises reactor body, solar collector, solar cell lighting system, carbonic acid gas plenum system, control device; Wherein, reactor body arranged outside has photoconductive fiber, and photoconductive fiber is connected with solar collector; Control device is controlled respectively the work of solar illuminating system and carbonic acid gas plenum system; In reactor body, be provided with temperature sensor, pH sensor, CO 2/ O 2sensor, temperature sensor, pH sensor, CO 2/ O 2sensor is connected with control device respectively.
2. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, reactor body is made with the pmma material of heat-resistant pressure-resistant.
3. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, solar cell lighting system comprises solar panel, store battery, LED auxiliary lighting apparatus, LED light source.
4. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, solar collector is comprised of collecting unit, and each collecting unit comprises condenser lens and photoconductive fiber.
5. the micro-algae bio-reaction system of a kind of sun power according to claim 4, it is characterized in that, photoconductive fiber adopts the superbright entirely lighting plastics multimode optical fibers 20-100 root of 1-5mm, and one end connects solar collector, and one end is centered around reactor body and carries out light filling around.
6. the micro-algae bio-reaction system of a kind of sun power according to claim 4, it is characterized in that, each 30 of the superbright entirely lighting plastics multimode optical fiberss of photoconductive fiber employing 2mm and 3mm, are used in combination, one end connects solar collector, and one end is centered around reactor body and carries out light filling around.
7. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, carbonic acid gas plenum system comprises carbon dioxide generating device, peristaltic pump, gas transmission pipeline, liquid transmission line.
8. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, reactor body below is also provided with heating jacket.
9. the micro-algae bio-reaction system of a kind of sun power according to claim 1, is characterized in that, reactor body is also connected with chilled water tank, and control device is controlled the cooling water flow of chilled water tank.
CN201410251520.XA 2014-06-09 2014-06-09 Solar microalgae biological reaction system Pending CN103981089A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106635782A (en) * 2016-11-30 2017-05-10 黑龙江省能源环境研究院 Oleaginous microalgae integrated culture reactor and culture method
CN110697986A (en) * 2019-10-25 2020-01-17 北京城市排水集团有限责任公司 Bacteria-algae coupling sewage treatment device based on energy recycling and use method thereof
CN113455246A (en) * 2021-08-04 2021-10-01 毋俊华 Environment-friendly system for absorbing carbon dioxide gas
GB2614560A (en) * 2022-01-07 2023-07-12 Nature Based Solutions Global Ltd Algae cultivation apparatus

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CN201198474Y (en) * 2008-04-02 2009-02-25 林健峯 Apparatus for cultivating oleaginous microalgae
KR20090038313A (en) * 2007-10-15 2009-04-20 주식회사 바이오트론 High efficiency photo-bioreactor for culturing micro algae
CN101550394A (en) * 2009-05-15 2009-10-07 武汉钢铁(集团)公司 Airlift type photobioreactor
CN101709262A (en) * 2009-12-10 2010-05-19 中国科学院广州能源研究所 Solar spectral photosynthetic bioreactor system for culturing microalgae in high density
CN202440492U (en) * 2012-02-23 2012-09-19 北京昊业怡生科技有限公司 Photobiological reactor for culturing oil-producing microalgae

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Publication number Priority date Publication date Assignee Title
KR20090038313A (en) * 2007-10-15 2009-04-20 주식회사 바이오트론 High efficiency photo-bioreactor for culturing micro algae
CN201198474Y (en) * 2008-04-02 2009-02-25 林健峯 Apparatus for cultivating oleaginous microalgae
CN101550394A (en) * 2009-05-15 2009-10-07 武汉钢铁(集团)公司 Airlift type photobioreactor
CN101709262A (en) * 2009-12-10 2010-05-19 中国科学院广州能源研究所 Solar spectral photosynthetic bioreactor system for culturing microalgae in high density
CN202440492U (en) * 2012-02-23 2012-09-19 北京昊业怡生科技有限公司 Photobiological reactor for culturing oil-producing microalgae

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106635782A (en) * 2016-11-30 2017-05-10 黑龙江省能源环境研究院 Oleaginous microalgae integrated culture reactor and culture method
CN110697986A (en) * 2019-10-25 2020-01-17 北京城市排水集团有限责任公司 Bacteria-algae coupling sewage treatment device based on energy recycling and use method thereof
CN110697986B (en) * 2019-10-25 2021-10-15 北京城市排水集团有限责任公司 Bacteria-algae coupling sewage treatment device based on energy recycling and use method thereof
US11702354B2 (en) 2019-10-25 2023-07-18 Beijing Drainage Group Co., Ltd Bacterium-alga coupled sewage treatment device based on energy recycling and use method thereof
CN113455246A (en) * 2021-08-04 2021-10-01 毋俊华 Environment-friendly system for absorbing carbon dioxide gas
GB2614560A (en) * 2022-01-07 2023-07-12 Nature Based Solutions Global Ltd Algae cultivation apparatus
GB2614560B (en) * 2022-01-07 2024-02-07 Nature Based Solutions Global Ltd Algae cultivation apparatus

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Application publication date: 20140813