CN204097489U - A kind of built-in light source bio-reactor and production cultivation equipment - Google Patents
A kind of built-in light source bio-reactor and production cultivation equipment Download PDFInfo
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- CN204097489U CN204097489U CN201420360069.0U CN201420360069U CN204097489U CN 204097489 U CN204097489 U CN 204097489U CN 201420360069 U CN201420360069 U CN 201420360069U CN 204097489 U CN204097489 U CN 204097489U
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Abstract
The utility model discloses a kind of built-in light source bio-reactor.This built-in light source bio-reactor comprises: reaction vessel, and reaction vessel is provided with cover plate and reaction vessel holds the nutrient solution supplying micro algae growth; Charging/discharging device, charging/discharging device and reaction vessel are tightly connected, and charging/discharging device is provided with valve and switch, and micro-algae is pumped into by charging/discharging device or pumps reaction vessel; Light-emitting device, for generation of the light source needed for micro algae growth; Nutrition distribution device, nutrition distribution device is used for the nutrition provided in reaction vessel needed for micro algae growth; And gas distributor, gas distributor is used for the gas provided in reaction vessel needed for micro algae growth.Built-in light source bio-reactor of the present utility model is not subject to the impact of Changes in weather and micro-algae propagation can be made to carry out stage by stage, controllability is stronger, be conducive to realizing the stable factorial praluction continued, the series connection of bio-reactor and/or parallel system make the large-scale farming of micro-algae become more efficient.
Description
Technical field
The utility model relates to biological technical field, is specifically related to built-in light source bio-reactor.
Background technology
Microalgae biodiesel is the future thrust of liquid biofuel, has that energy conversion efficiency is high, compared with common Lu Sheng farm crop, the productive rate of unit surface can exceed decades of times, thus can realize industrialized culture.The principle of micro-algae liquefaction utilizes micro-algae photosynthesis, the carbon dioxide conversion produced in chemical process is the biomass of micro-algae self thus secures carbon, the carbon species of micro-algae self is made to be converted into grease by induced reaction again, then utilize physics or chemical process that the grease in microalgae cell is transformed into extracellular, carry out refinement processing again, thus produce biofuel, namely by the photosynthesis of algae, be biofuel, protein by the nutritive substance in waste water and gas and carbon dioxide conversion.Significantly rise in oil price, today that food shortage problem is outstanding day by day, this industry has vast potential for future development.
Cultivation for the production of micro-algae of biofuel is mainly divided into two stages, i.e. reproductive stage and produce oil stage.At the reproductive stage of both culturing microalgae, micro-algae dominant absorption reddish yellow light and royal purple light (wavelength region is at 620-700nm and 410-470nm), the elements such as simultaneously stability carbon, nitrogen, phosphorus, carry out breeding and not produce oil; In the produce oil stage of both culturing microalgae, no longer pass into nitrogenous source and keep passing into of a small amount of phosphorus and continue to pass into CO
2, keep illumination, micro-algae because of rich carbon and nitrogen hunger thus produce oil efficiency obviously increase, and reproduction speed significantly reduces.
For improve further microalgae biodiesel unit output, realize three dimensional-breeding, special both culturing microalgae photoreactor must be used.The outdoor direct cultivation such as raceway pond, multistage pond although both culturing microalgae photoreactor of the prior art is compared, cultivation density, yield per unit are all significantly improved, but because the light source of micro algae growth and produce oil is still natural light, micro-algae is insufficient to the utilization of sunlight, there is uncertain and uncontrollability, the requirement stablize with factorial praluction, continued runs in the opposite direction completely, in addition the auxiliary breeding function also unrealized automatization such as nutritional supplementation, carbonic acid gas inflation, makes itself and actual commercial application still have larger distance.
Therefore, current this area can overcome poor controllability, low, the inefficient drawback of level of automation of above-mentioned both culturing microalgae photoreactor of the prior art in the urgent need to a kind of technology.
Utility model content
The purpose of this utility model be to provide a kind of can according to the built-in light source bio-reactor of the key element such as light source, carbonic acid gas, nutrition, temperature, flow velocity of cultivation demand proportioning the best of different sorts algae different steps, thus realize the stable, controlled of super-high density algal cultivation, meet industrialization demand.
According to an aspect of the present utility model, provide a kind of built-in light source bio-reactor, this built-in light source bio-reactor comprises:
Reaction vessel, reaction vessel is provided with cover plate and reaction vessel holds the nutrient solution supplying micro algae growth;
Charging/discharging device, charging/discharging device and reaction vessel are tightly connected, and charging/discharging device is provided with valve and switch;
Light-emitting device, light-emitting device is located at reaction vessel interior and when reactor works, the nutrient solution that this light-emitting device is immersed at least partly or all, thus the light needed for micro algae growth is provided in reaction vessel, wherein the light intensity of light that sends of light-emitting device is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel; With
Gas distributor, gas distributor is used for the gas provided in reaction vessel needed for micro algae growth.
In another preference, the light intensity of the light that light-emitting device sends is substantially even in the horizontal direction (comprising X-axis and Y direction) of reaction vessel.
In another preference, described " uniformly or roughly uniform " refers to the light intensity D1 of arbitrary depth and meets following formula at the average intensity Dm of entire depth scope:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8; More preferably 1.1 >=D1/Dm >=0.9.
In another preference, the surrounding of reaction vessel is provided with temperature control unit, and this temperature control unit is in the scope of applicable micro algae growth for the liquid environment temperature maintained in reaction vessel.
In another preference, reactor is also provided with nutrition distribution device, and this nutrition distribution device is used for the nutrition provided in reaction vessel needed for micro algae growth.
In another preference, this temperature control unit is temperature control water pipe.
In another preference, the scope being applicable to micro algae growth refers to 15-45 DEG C, preferably 20-40 DEG C.
In another preference, built-in light source bio-reactor is also provided with Monitoring systems, and for the parameter of monitoring liquid environment, parameter is selected from: pH value, temperature and/or nutriment concentration.
In another preference, light-emitting device comprises light guiding plate and luminescence unit, and the light transmission light guiding plate conduction that luminescence unit produces makes light guiding plate Integral luminous.
In another preference, light-emitting device also comprises support, and support is used for fixed support light guiding plate, and support is removably connected with reaction vessel and/or cover plate, and luminescence unit is embedded in light guiding plate.
In another preference, be on the cover board provided with production well.
In another preference, this luminescence unit is LED luminescence unit.
In another preference, be provided with polylith light guiding plate in this built-in light source bio-reactor, preferably 3-100 block, more preferably 4-80 block, best 5-50 block.
In another preference, light guiding plate is made up of the transparent organic material with anti-weak acid performance.
In another preference, during light-emitting device work, launch the light of at least 2 different wave lengths.
In another preference, the light of aforementioned different wave length comprises: the light that the light that wavelength is 600-800 (preferably 650-750) nm, wavelength are 400-480 (preferably 430-470) nm.
In another preference, the colour temperature of the light that luminescence unit sends is 1000-20000K, preferably 1500-6000K.
In another preference, in light-emitting device, luminescence unit is LED, and the quantity of LED is 1-10000/ light guiding plate; It is preferably 10-1000/ light guiding plate.
In another preference, gas distributor is rotary gas sparger, is passing in the process of gas in reaction vessel, and rotary gas sparger rotates, thus impels gas and nutritive substance to be scattered in liquid culture system.
According to second aspect of the present utility model, provide a kind of production cultivation equipment, this production cultivation equipment comprises the built-in light source bio-reactor of the utility model first aspect.
In another preference, at least two built-in light source bio-reactors are series connection and/or parallel connection.
In another preference, interconnective reactor is connected by charging/discharging device.
In another preference, this production cultivation equipment is used as the production cultivating system of the micro-algae of Chlorophyta, Cyanophyta, Chrysophyta and rhodophyta.
According to the third aspect of the present utility model, provide a kind of cultural method, the method comprising the steps of:
A () provides aforesaid built-in light source bio-reactor;
B () cultivates micro-algae in built-in light source bio-reactor.
According to fourth aspect of the present utility model, provide a kind of method of micro-algae for the preparation of production biodiesel, the method comprising the steps of:
A () provides aforesaid built-in light source bio-reactor;
B () cultivates the micro-algae for the production of biodiesel in built-in light source bio-reactor,
Wherein, cultivate and comprise the first cultivation stage and the second cultivation stage, in the first stage of micro algae growth, the wavelength of the light that luminescence unit sends is 350-900nm, preferably 570-800 and 400-500nm; In the subordinate phase of micro algae growth, the wavelength of the light that luminescence unit sends is 350-900nm, preferably 600-800 and 400-480nm;
C () reclaims the micro-algae cultivated from built-in light source bio-reactor.
In another preference, in the first stage of micro algae growth, the wavelength that luminescence unit sends is 570-800nm; In the subordinate phase of micro algae growth, the wavelength that luminescence unit sends is 400-480nm.
In another preference, in the first stage of micro algae growth, in built-in light source bio-reactor, provide nitrogenous source; In the subordinate phase of micro algae growth, stop providing nitrogenous source in built-in light source bio-reactor.
In another preference, method also comprise: to reclaim micro-algae process (such as drying, broken wall, extraction, transesterify etc.) thus obtain biodiesel.
According to the 5th aspect of the present utility model, provide a kind of micro-algae that can be used for producing biodiesel, this micro-algae is prepared by the method described in fourth aspect.
In another preference, this micro-algae has following characteristic:
I the size of () micro-algae is 5-500 micron;
(ii) oil length (dry weight) 10%-70%.
According to the 6th aspect of the present utility model, provide a kind of method preparing biodiesel, it is characterized in that, comprise step: utilize micro-algae described in the utility model the 5th aspect as raw material, process, thus obtained biodiesel.
In another preference, the processing of micro-algae comprises: the processing steps such as drying, extraction, extraction, transesterify.
Accompanying drawing explanation
Fig. 1 is the three-dimensional cutaway view of the built-in light source bio-reactor according to the utility model embodiment;
Fig. 2 is the perspective cross-sectional schematic diagram of the built-in light source bio-reactor according to the utility model embodiment;
Fig. 3 looks cross-sectional schematic according to the master of the built-in light source bio-reactor of the utility model embodiment;
Fig. 4 overlooks cross-sectional schematic according to the built-in light source bio-reactor of the utility model embodiment;
Fig. 5 is the schematic perspective view of the light-emitting device of built-in light source bio-reactor according to the utility model embodiment;
Fig. 6 a is the front view of the light-emitting device of built-in light source bio-reactor according to the utility model embodiment;
Fig. 6 b is the side-view of the light-emitting device of built-in light source bio-reactor according to the utility model embodiment;
Fig. 6 c is the vertical view of the light-emitting device of built-in light source bio-reactor according to the utility model embodiment; And
Fig. 6 d is the enlarged view according to part B in Fig. 6 b.
Embodiment
Contriver is through extensive and deep research, develop a kind of built-in light source bio-reactor first, by structures such as the light-emitting devices of particular design, reactor of the present utility model not only shortens incubation time, and the biomass density of micro-algae and total effectively fat content can be significantly improved, thus the better biofuel of quality can be prepared.Complete the utility model on this basis.
Below with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail, understands the purpose of this utility model, feature and advantage so that clearer.It should be understood that embodiment shown in the drawings is not the restriction to the utility model scope, and the connotation just in order to technical solutions of the utility model are described.
Bio-reactor
As depicted in figs. 1 and 2, described built-in light source bio-reactor comprises reaction vessel 1, light-emitting device 2, charging/discharging device 3, cover plate 5, gas distributor 6, nutrition distribution device, temperature control unit 7 and monitor and forecast system etc.Reaction vessel 1 is provided with cover plate 5, reaction vessel 1 and charging/discharging device 3 are tightly connected, and charging/discharging device is provided with valve and switch, for controlling the carrying out of input and output material or stopping, light-emitting device 2 is provided with for generation of the stabilized light source needed for micro algae growth each stage in reaction vessel 1, the bottom of reaction vessel 1 is provided with gas distributor 6 for passing into the gas needed for micro algae growth each stage, nutrition distribution device is used for supplying nutrients in reaction vessel, the surrounding of reaction vessel is provided with temperature control unit 7 (being illustrated as temperature control water pipe) for keeping the temperature of solution in reaction vessel in the temperature range of applicable micro-algae propagation and produce oil.
The bottom (height of according to appointment 30% ~ 90%, or 50% ~ 70%) of this bio-reactor can be embedded in underground.
This bio-reactor can be arranged on around power station, and the waste water and gas utilizing generating to produce is originated as the matter and energy of both culturing microalgae, has the effect of environmental emission reduction.This reaction vessel 1 can be lighttight encloses container, also can carry out luminescence by the light-emitting device of self overcast and rainy with night, used for micro algae growth.Monitoring systems is provided with, for solution pH value and temperature etc. in monitoring reactor in built-in light source bio-reactor.
In addition, built-in light source bio-reactor of the present utility model also can be used for an algae, dinoflagellate or other hydrobiological high-density industrialization cultivation.
Fig. 3 is that the master of this built-in light source bio-reactor looks cross-sectional schematic; As shown in Figure 3, be provided with temperature control unit 7 on the walls of the reaction vessel, temperature control unit 7 can be any suitable form, such as temperature control spacer.Preferably, temperature control unit 7 is temperature control water pipe, and the waste water with certain temperature that power station is discharged is used in temperature control water pipe and circulates to keep temperature in bio-reactor between 15 DEG C ~ 45 DEG C.
Production well is provided with, for discharging the oxygen that micro-algae photosynthesis produces at the top of the cover plate 5 of bio-reactor.
Fig. 4 be this built-in light source bio-reactor overlook cross-sectional schematic.As shown in the figure, be provided with gas distributor 6 in the bottom of built-in light source bio-reactor, gas distributor 6 can be rotary, slot type, tubular type or other any suitable forms.In microalga cultivation process, in reaction vessel, pass into carbonic acid gas, air or other gas by gas distributor.Preferably, adopt rotary gas sparger in this example, in the process passing into gas, gas distributor 6 rotarily drives the disturbance of liquid in the gas and bottom container that pass into, thus is conducive to gas and nutritive substance is uniformly distributed in the solution.
In one or more positions (on such as wall of container, inside container bottom, container top and cover plate etc.) of reaction vessel, nutrition distribution device is installed.Nutrition distribution device provides different nutriment in the different steps of micro algae growth.
In reaction vessel, other nutritive substances passes into speed and CO
2pass into the ratio that speed can become certain, pass through CO
2on-line monitoring system can determine CO in solution
2meltage, know the potential of hydrogen of solution by the pH value of Monitoring systems monitoring solution simultaneously.The CO that Controlling System can control
2with nutritive substance pass into speed, ensure that the meltage of solution Middle nutrition material and pH value are in the scope of applicable micro algae growth breeding or produce oil.
Light-emitting device
Fig. 5 is the schematic perspective view of light-emitting device 2.Light-emitting device be arranged so that bio-reactor can when carrying out the normal operations such as breeding production without when sunlight or other external light sources.As described in Figure 5, described light-emitting device is made up of support 9, LED luminescence unit group 10 and light guiding plate 11, and light guiding plate 11 is fixed by support 9, and the top of support 9 is fixed on cover plate 5, LED luminescence unit group 10 is positioned at the top of light guiding plate 11, light guiding plate 11 or all stretch under liquid level.In bio-reactor of the present utility model, be provided with multiple light-emitting device 2, each light guiding plate all can install or remove individually.
Light guiding plate can be made with transparent organic material (such as acrylic), and has anti-weak acid performance.LED luminescence unit group directly contacts with light guiding plate at the top of light guiding plate, and the light that LED luminescence unit group sends can pass through light guiding plate and conducts and make light guiding plate Integral luminous.
The top of the support 9 of light-emitting device 2 is removably connected with cover plate 5, thus be convenient to light-emitting device dismounting, change or reinstall; Light-emitting device 2 is suspended on the bottom of not contacting container in reaction vessel, does not thus affect the rotation of container bottom gas distributor.Should be understood that the support of light-emitting device also can be fixed in any suitable position and reaction vessel in other any suitable modes.
Fig. 6 a-6c is the front view of the light-emitting device of built-in light source bio-reactor, side-view and vertical view respectively; Fig. 6 d is the enlarged view of part B in Fig. 6 b.
LED luminescence unit group 10 comprises LED luminescence unit little one by one, and the light that luminescence unit produces can be tertiary colour or monochromatic ray, and monochromatic LED emission wavelength range is 350-900nm, and complex light reference color temperature is 1500-20000K.The quantity of the LED luminescence unit on single light guiding plate can be 1-10000.
Wherein the light intensity of light that sends of light-emitting device is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel; The light intensity of the light that light-emitting device sends is substantially even in the horizontal direction (comprising X-axis and Y direction) of reaction vessel." uniformly or roughly uniform " refers to the light intensity D1 of arbitrary depth and meets following formula at the average intensity Dm of entire depth scope:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8; More preferably 1.1 >=D1/Dm >=0.9.
The light of at least 2 different wave lengths can be launched during light-emitting device work.The light of this different wave length comprises: wavelength is the light of 570-800nm, wavelength is the light of 400-500nm.
In fact, LED luminescence unit group 10 can be positioned at the bottom of light guiding plate 11 or be embedded in other suitable positions of luminescent screen, makes monoblock light guiding plate luminous as long as ensure that its light sent can conduct in monoblock light guiding plate.
Preferably, the energy derive of LED luminescence unit group is the electric energy that sun power tabula rasa absorption sun power produces.So, though bio-reactor of the present utility model does not directly utilize sun power, but by sun power tabula rasa, the solar energy collecting of instability is got up for generating, the electricity produced stably is supplied to the luminescence unit group of bio-reactor to make it luminous constantly, ensures stable, the persistence of both culturing microalgae.
Produce cultivating system
The breeding of micro-algae and produce oil stage can carry out in same bio-reactor, but need the carrying out along with growth, change light-emitting device, and the kind that change nutritive substance passes into and speed, process is comparatively complicated.
In order to realize efficient large-scale farming, multiple bio-reactor serial or parallel connection can be adopted to form and to produce cultivating system on a large scale.Serial or parallel connection between bio-reactor is connected by feed inlet and outlet, can complete charging between bio-reactor and discharging by pump system.
Consider the number change in microalga cultivation process, the demand of light source and nutritive substance is also constantly being changed, the cultivating system that multiple bio-reactor is connected can be adopted, such as, adopt the system of three bio-reactor series connection to complete whole process of growth of micro-algae propagation and produce oil:
Appropriate algae kind is put into first step bio-reactor, and breeding is increased to some amount, enters second stage bio-reactor carry out further growth breeding by feed inlet and outlet, then enter third stage bio-reactor again, no longer breed, only mainly carry out produce oil, and less breeding.
The light-emitting device that emission wavelength is only applicable to being conducive to micro-algae propagation is provided with in the first step and second stage bio-reactor, and nutrition distribution device passes into the oxynitride such as ammonium phosphate, potassium primary phosphate or dipotassium hydrogen phosphate as nitrogen phosphorus source in reaction vessel, add the element such as iron, zinc simultaneously, gas distributor passes into oxynitride and CO2 in reaction vessel, in the bio-reactor of the second stage luminous intensity and nutritive substance delivery rate large compared with first step bio-reactor, to adapt to the growth demand of breeding micro-algae of increasing; The light-emitting device that emission wavelength is only applicable to being conducive to micro-algae produce oil is provided with in third stage bio-reactor, PH and temperature etc. are adjusted to the condition of applicable micro-algae produce oil simultaneously, and nutrition distribution device does not provide nitrogenous source, gas distributor passes into CO2 in reaction vessel.
Bio-reactor not at the same level can select different sizes according to demand, and the comparatively rear two-stage of the such as size of first step bio-reactor is less.
After micro-algae in first step bio-reactor is expelled to second stage bio-reactor, new algae kind can be passed into first step bio-reactor, carry out new round cultivation simultaneously.In order to improve the purity of algae kind, the micro-algae can discharged from second stage bio-reactor, filter out the algae kind of part as first step bio-reactor.
Cultural method
The utility model provides a kind of method of cultivating micro-algae, and the method comprising the steps of:
1. built-in light source bio-reactor of the present utility model is provided;
2. algae kind is invested in this built-in light source bio-reactor, nutriment (comprising nitrogenous source, phosphorus source, inorganic salt (such as iron, zinc) etc.) needed for the existence of micro-algae be provided in reactor and carbonic acid gas or air are provided, opening the light of light-emitting device generation needed for micro algae growth.
Especially, the utility model provides a kind of method for cultivating the micro-algae producing biodiesel, and the method comprising the steps of:
1. built-in light source bio-reactor of the present utility model is provided;
2. algae kind is invested in this built-in light source bio-reactor, nutriment (comprising nitrogenous source, phosphorus source, inorganic salt (such as iron, zinc) etc.) needed for the existence of micro-algae is provided in reactor and carbonic acid gas or air are provided, open the light of light-emitting device generation needed for micro algae growth, wherein
In the first stage of micro algae growth, the wavelength of the light that luminescence unit sends is 350-900nm, preferably 570-800 and 400-500nm; In the subordinate phase of micro algae growth, the wavelength of the light that luminescence unit sends is 350-900nm, preferably 600-800 and 400-480nm.
Preferably, adopt the light of different wave length as light source in first stage of micro algae growth and subordinate phase, coordinate the supply of nutritive substance and gas, micro-algae was mainly bred in the first stage, mainly carries out produce oil in subordinate phase.Such as, adopt ruddiness to pass into nitrogenous source as light source in the first stage, and subordinate phase adopt blue light as light source and stop passing into nitrogenous source.
It is applicable to microorganism of the present utility model be not particularly limited, as long as can utilize light source to grow.Representational microorganism comprises (but being not limited to): the micro-algae of Chlorophyta, Cyanophyta, Chrysophyta and rhodophyta.A kind of preferred microorganism is Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species.
Preparation method
The utility model provides a kind of method preparing biodiesel, and the method adopts the micro-algae for the production of biodiesel of being prepared by preceding method as raw material, processes, thus obtained biodiesel, a kind of typical course of processing comprises step:
The method utilizing filtration, press filtration or bubble to suspend collects micro-algae, then by being separated algae oil and frond after the method broken wall of cellulase hydrolysis and homogeneous, through extraction, leach or obtain after press filtration pure algae oil, after adopting ester-interchange method, solvent cut method or thermal decomposition method, obtain the biofuel that can be directly used in diesel motor.
Advantage of the present utility model
Compared to prior art, the utility model has following major advantage:
1. bio-reactor of the prior art depends on natural light outside, and under the weather condition that overcast and rainy isocandela is more weak, micro-algae cannot obtain sufficient light source and slow down growth, and built-in light source bio-reactor of the present utility model is not by the impact of Changes in weather;
2. prior art is not suitable for big pool cultivated, because only have the micro-algae close to pond upper surface could obtain more sufficient light source, and built-in multiple equally distributed light-emitting device in bio-reactor of the present utility model, and gas distributor in bio-reactor of the present utility model and nutrition distribution device are all conducive to being uniformly distributed of nutritive substance in reaction vessel, make it possible to realize big pool cultivated, three-dimensional culture, improve cultivation efficiency;
3. bio-reactor of the present utility model can adopt the condition such as different built-in light sources and nutrition, temperature, PH according to the Different growth phases of micro-algae, and micro-algae propagation is carried out stage by stage, and controllability is comparatively strong, is conducive to realizing the stable factorial praluction continued;
4. the series connection of bio-reactor of the present utility model and/or parallel system make the large-scale farming of micro-algae become more efficient.
Below in conjunction with specific embodiment, set forth the utility model further.Should be understood that these embodiments are only not used in restriction scope of the present utility model for illustration of the utility model.The experimental technique of unreceipted actual conditions in the following example, usually conveniently condition, or according to the condition that manufacturer advises.Unless otherwise indicated, otherwise per-cent and number are weight percent and parts by weight.
In embodiment 1-3, adopt built-in light source bio-reactor of the present utility model as shown in Figure 1.
Embodiment 1
Choose conventional Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species, initial cultivation density is 0.1g/L, and reactor level is 80cm (sectional area is 1m × 1.5m).First stage adopts the red-light LED of wavelength 706nm to be light source, and homo(io)thermism is 24 DEG C, adds micro-ammonium phosphate and the trace element fertilizer such as zinc, iron in advance, and pass into a certain amount of CO respectively in nutrient solution
2(about 90%) and NO or NO as nitrogenous source
2put into after one hour and put into algae kind Deng oxynitride by initial cultivation density, start cultivation.Within 36 hours, record biomass density later and increase to 6.86g/L (dry weight/solution, lower same), biomass density 9.71g/L is recorded after 72 hours, algae micro-in reactor is moved to another reactor, and (level is 80cm, sectional area is 2m × 3m) in, stop oxynitride supply, keep CO
2supply, the blue-ray LED that wavelength is 450nm is used to be light source, homo(io)thermism is 28 DEG C, moves to another reactor after 24 hours, and water sampling detects, nitrogenous source exhausts substantially, biomass density is 10.64g/L, and move to another reactor after 72 hours, biomass density increases to 12.88g/L, cultivation terminates, and amounts to 144 hours.
In visual inspection reactor, micro-algae is evenly distributed.Obtain micro-algae after filtration, after detecting, total lipid content (dry weight) is 45.08%, and triacylglycerol fatty acid ester (triglyceride level) accounts for total lipid content 89.6%, total effectively fat content 40.39%, total effectively fat content density 5.20g/L.
Embodiment 2
Choose Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species, initial cultivation density is 0.1g/L, and reactor level is 80cm (sectional area is 1m × 1.5m).First stage adopts the blue-ray LED of wavelength 450nm to be light source, and homo(io)thermism is 24 DEG C, adds micro-ammonium phosphate and the trace element fertilizer such as zinc, iron in advance, and pass into a certain amount of CO respectively in nutrient solution
2(about 90%) and NO or NO as nitrogenous source
2put into after one hour and put into algae kind Deng oxynitride by initial cultivation density, start cultivation.Within 36 hours, record biomass density later and increase to 5.66g/L, record biomass density 8.03g/L after 72 hours, algae micro-in reactor is moved in another reactor (level is 80cm, and sectional area is 2m × 3m), stop oxynitride supply, keep CO
2supply, the red-light LED using wavelength 706nm is light source, homo(io)thermism is 30 DEG C, moves to another reactor after 24 hours, and water sampling detects, nitrogenous source exhausts substantially, biomass density is 9.21g/L, and move to another reactor after 72 hours, biomass density increases to 10.52g/L, cultivation terminates, and amounts to 144 hours.
In visual inspection reactor, micro-algae is evenly distributed.Obtain micro-algae after filtration, after detecting, total lipid content (dry weight) is 43.98%, and triacylglycerol fatty acid ester (triglyceride level) accounts for total lipid content 77.7%, total effectively fat content 34.17%, total effectively fat content density 3.59g/L.
Embodiment 3
Choose Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species, initial cultivation density is 0.1g/L, and reactor level is in 80cm (sectional area is 2m × 3m).First stage adopts the red-light LED of wavelength 706nm to be light source, and homo(io)thermism is 24 DEG C, adds micro-ammonium phosphate and the trace element fertilizer such as zinc, iron in advance, and pass into a certain amount of CO respectively in nutrient solution
2(about 90%) and NO or NO as nitrogenous source
2put into after one hour and put into algae kind Deng oxynitride by initial cultivation density, start cultivation.By certain hour sampling, measure biomass density.After 144 hours, cultivation terminates.
Result: record biomass density after 36 hours and increase to 6.86g/L, recording biomass density after 72 hours is 9.91g/L, and recording biomass density after 108 hours is 12.22g/L, and after 144 hours, cultivation records final biomass density after terminating is 14.34g/L.In visual inspection reactor, micro-algae is evenly distributed.Obtain micro-algae after filtration, after detecting, total lipid content (dry weight) is 24.52%, and triacylglycerol fatty acid ester (triglyceride level) accounts for total lipid content 81.6%, total effectively fat content 20.01%, total effectively fat content density 2.45g/L.
Comparative example 1
Choose Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species, initial cultivation density is 0.1g/L, and reactor level is in 80cm (sectional area is 2m × 3m).Employing natural light is light source, Laboratory culture, adds micro-ammonium phosphate and the trace element fertilizer such as zinc, iron in advance, and pass into a certain amount of CO respectively in nutrient solution
2(about 90%) and NO or NO as nitrogenous source
2deng oxynitride, after 144 hours, cultivation terminates.Unless stated otherwise, other culture condition is with embodiment 1.
Result: the micro-algae density in naked-eye observation liquid level surface is obviously larger, and forms Guinier-Preston zone, records final average organism metric density and is only 3.01g/L, total effectively fat content 19.99%, total effectively fat content density is only 0.60g/L.
Comparative example 2
Choose Nannochloropsis oceanica (Nannochloropsis limnetica) fresh water species, initial cultivation density is 0.1g/L, and reactor level is 80cm (sectional area is 2m × 3m).Employing natural light is light source, Laboratory culture, adds micro-ammonium phosphate and the trace element fertilizer such as zinc, iron in nutrient solution in advance, and passes into a certain amount of air and NO or NO as nitrogenous source respectively
2deng oxynitride, after 144 hours, cultivation terminates.Unless stated otherwise, other culture condition is with embodiment 1.
Result: the micro-algae density in naked-eye observation liquid level surface is obviously larger, and forms Guinier-Preston zone, records final average organism metric density and is only 1.29g/L, total effectively fat content 28.32%, total effectively fat content density is only 0.37g/L.
More clearly, the part of test results of above-described embodiment and comparative example contrasts as shown in table 1:
Table 1
| ? | Biomass density (g/L) | Total effectively fat content (%) | Total effectively fat content density (g/L) |
| Embodiment 1 | 12.88 | 40.39 | 5.20 |
| Embodiment 2 | 10.52 | 34.17 | 3.59 |
| Embodiment 3 | 14.34 | 20.01 | 2.45 |
| Comparative example 1 | 3.01 | 19.99 | 0.60 |
| Comparative example 2 | 1.29 | 28.32 | 0.37 |
Conclusion
The experimental result of embodiment above and comparative example can be found out:
1. adopt the utility model embodiment of built-in light source compared to the comparative example adopting natural light as light source, through the identical time (144 hours), as shown in table 1, biomass density adds about 3-10 doubly, total effectively fat content density adds about 4-14 doubly, and cultivation efficiency and produce oil efficiency all significantly improve;
2. in embodiment 1 and embodiment 2, both culturing microalgae carries out in different bio-reactors stage by stage and different steps adopts different light sources, in embodiment 3, micro-algae cultivates all the time under the same conditions, as can be seen from the result of these three embodiments, the total lipid content that micro-algae cultivates stage by stage and always effective fat content are all higher, and produce oil efficiency is high;
3. embodiment 1 is with the difference of embodiment 2, in embodiment 1, the first stage adopts ruddiness as light source, subordinate phase adopts blue light, in embodiment 2, the first stage adopts blue light, subordinate phase adopts ruddiness, as can be seen from experimental result, the culture efficiency of embodiment 1 is better than embodiment 2, can think, adopt the light source of different wave length can cause the difference of result for different algae kind in first stage of both culturing microalgae and subordinate phase, that be applicable to, different light sources can be selected according to algae kind feature respectively in above-mentioned two stages, thus improve integral production efficiency.
The all documents mentioned at the utility model are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the utility model after having read above-mentioned teachings of the present utility model, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a built-in light source bio-reactor, is characterized in that, described built-in light source bio-reactor comprises:
Reaction vessel, described reaction vessel is provided with cover plate and described reaction vessel holds the nutrient solution supplying micro algae growth;
Charging/discharging device, described charging/discharging device and described reaction vessel are tightly connected;
Light-emitting device, described light-emitting device is located at reaction vessel interior and when the work of described reactor, described light-emitting device is immersed in described nutrient solution at least partly or all, thus the light needed for micro algae growth is provided in reaction vessel, the light intensity of the light that wherein said light-emitting device sends is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel; With
Gas distributor, described gas distributor is used for the gas provided in described reaction vessel needed for micro algae growth.
2. built-in light source bio-reactor as claimed in claim 1, it is characterized in that, the surrounding of described reaction vessel is provided with temperature control unit, and described temperature control unit is in the scope of applicable micro algae growth for the liquid environment temperature maintained in reaction vessel; And/or
Described reactor is also provided with nutrition distribution device, and described nutrition distribution device is used for the nutrition provided in described reaction vessel needed for micro algae growth.
3. built-in light source bio-reactor as claimed in claim 1, it is characterized in that, built-in light source bio-reactor is also provided with Monitoring systems, and for monitoring the parameter of described liquid environment, described parameter is selected from: pH value, temperature and/or nutriment concentration.
4. built-in light source bio-reactor as claimed in claim 1, it is characterized in that, described light-emitting device comprises light guiding plate and luminescence unit, and the light transmission light guiding plate conduction that described luminescence unit produces makes light guiding plate Integral luminous.
5. built-in light source bio-reactor as claimed in claim 4, it is characterized in that, described light-emitting device also comprises support, and described support is used for light guiding plate described in fixed support, described support is removably connected with described reaction vessel and/or described cover plate, and described luminescence unit is embedded in described light guiding plate.
6. built-in light source bio-reactor as claimed in claim 5, is characterized in that, launches the light of at least 2 different wave lengths during described light-emitting device work.
7. built-in light source bio-reactor as claimed in claim 5, it is characterized in that, the colour temperature of the complex light that described luminescence unit sends is 1000-20000K, preferably 1500-6000K.
8. built-in light source bio-reactor as claimed in claim 5, it is characterized in that, in described light-emitting device, luminescence unit is LED, and the quantity of LED is 1-10000/ light guiding plate; It is preferably 10-1000/ light guiding plate.
9. built-in light source bio-reactor as claimed in claim 1, it is characterized in that, described gas distributor is rotary gas sparger, passing in the process of gas in reaction vessel, described rotary gas sparger rotates, thus impels gas and nutritive substance to be scattered in liquid culture system.
10. produce a cultivation equipment, it is characterized in that, described production cultivation equipment comprises two or more built-in light source bio-reactors according to claim 1.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017726A (en) * | 2014-06-30 | 2014-09-03 | 张玟籍 | Built-in light source bioreactor and microalgae culture method |
| CN105316235A (en) * | 2015-04-17 | 2016-02-10 | 上海希明生物科技有限公司 | Freshwater eukaryoticmicroalgae culture method |
| CN108138101A (en) * | 2015-07-29 | 2018-06-08 | 藻类研究中心公司 | Light emitting diode bioreactor and application method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017726A (en) * | 2014-06-30 | 2014-09-03 | 张玟籍 | Built-in light source bioreactor and microalgae culture method |
| CN105316235A (en) * | 2015-04-17 | 2016-02-10 | 上海希明生物科技有限公司 | Freshwater eukaryoticmicroalgae culture method |
| WO2016165660A1 (en) * | 2015-04-17 | 2016-10-20 | 上海希明生物科技有限公司 | Method for farming freshwater eukaryotic microalgae |
| CN108138101A (en) * | 2015-07-29 | 2018-06-08 | 藻类研究中心公司 | Light emitting diode bioreactor and application method |
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