CN202116547U - Dedicated incubator for algae cultivation and seed conservation - Google Patents

Dedicated incubator for algae cultivation and seed conservation Download PDF

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Publication number
CN202116547U
CN202116547U CN2011202358304U CN201120235830U CN202116547U CN 202116547 U CN202116547 U CN 202116547U CN 2011202358304 U CN2011202358304 U CN 2011202358304U CN 201120235830 U CN201120235830 U CN 201120235830U CN 202116547 U CN202116547 U CN 202116547U
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incubator
special
algae
guarantor
algae culture
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虞利俊
林家彬
余刚
陈俊
薄海豹
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

The utility model relates to a dedicated incubator for algae cultivation and seed conservation, which consists of an upper cover (2), a transparent box body (17) and a base (10). A light-emitting diode (LED) which is taken as the light source is arranged at the upper part of the transparent box body, a blade (5) is arranged at the lower part of the transparent box body, and a speed-reducing motor (12) which is arranged at the corresponding position outside the transparent box body provides power for the blade to rotate, thereby ensuring the uniform distribution of the algae in culture solution; a heating system is arranged between the base (10) and the transparent box body (17), so that the incubator is heated uniformly by the heat generated. The utility model has the advantages that the dedicated incubator is energy-saving, convenient, reliable and durable, can be heated uniformly, is convenient to dismantle and wash and can be used for a long time.

Description

Algae culture, guarantor's kind of special-purpose incubator
Technical field
The utility model is planted and cultured special automatic control incubator to the specially designed guarantor of algae, particularly tenaculat Habenaria characteristic, belongs to aquatic algae culture apparatus category.
Background technology
Phycophyta is the photosynthetic organism that occurs the earliest on the earth.Survived nearly 3,500,000,000 years, its cellularstructure is original simple, is in the bottom of whole food chain, if there is not algae in the water, the animalcule of the numerous species in the water all will be dead because there not being bait.Also just can't build huge biologic chain system.
The scope that algae distributes is extremely wide, envrionment conditions is required not tight, and flexibility is stronger, also can live having only under extremely low nutrient concentration, atomic weak intensity of illumination and the quite low temperature.Rivers, streams, lake and ocean can not only be grown in, and of short duration ponding or moist place can be grown in.From the torrid zone to the two poles of the earth, to warm spring, in not really dark soil, the algae that almost grows on trees distributes from damp ground from the high mountain of accumulated snow.
Kind surplus the known algae 30,000 in the whole world; Some phycophyta protein contnt can be up to 60%, and along with the continuous increase of population on the earth, the grain that needs also constantly increases; Land area is limited; Increasing crop yield is also limited, current global food problem occurred, and people turn one's attention to the algae in lake and the ocean for this reason.The mankind open to cheat phycophyta are developed and studies for this reason, to replenish the deficiency of human wants.Because in recent decades, scientist finds that many algae such as tenaculat Habenaria contain higher protein, biologically active substances such as VITAMINs, mineral substance and unsaturated fatty acids; Contain 8 kinds of amino acid that human body is necessary, and form rationally, can improve lymphocyte activity; Enhancing human immune; Become welcome protective foods in many developed countries, present international algae application in industry gets into the stage of commercially producing, and whole world YO reaches few hundred thousand tonnes of.Wherein, tenaculat Habenaria is our the most familiar edible algae.Tenaculat Habenaria is one type of lower plant, belongs to Cyanophyta, Oscillariaceae.They are the same with bacterium, do not have real nucleus in the cell, and there is a colourless central area protoplastis inside, the nucleus of similar other algae, but seedless Renhe nuclear membrane, so tenaculat Habenaria is the protokaryon plant.The tenaculat Habenaria that is usually used in cultivating has spirulina plalensis, spirulina maxim and salt pool tenaculat Habenaria.Spirulina plalensis is a filament, algal filament spirrillum, no cross wall, blue-greenish colour; Wide 4~5 μ m of algal filament, long 400~600 μ m; The blunt circle of the apical cell of algal filament, no heterocyst.。Except that tenaculat Habenaria, can if the growth of algae uncontrolled ground also can give rise to trouble certainly, be exactly an instance by human use's chlorella, Dunaliella salina, haematococcus pulvialis etc. in addition like the Taihu Lake blue algae bloom.
In algae research and utilizing, the cultivation of various algaes is bred, the algae kind is preserved is very important, owing to there is not this type of special-purpose plant and instrument; Most people are to use general plant incubator, triangular flask, shaking table, and thermostat containers etc. substitute; But because algal grown has its singularity, like specific illumination, temperature, pH value, nutrient substance, dissolved oxygen etc.; Also will water slowly mildly stirred up and down, this be because some algae has last buoyancy, like tenaculat Habenaria.When its growth when reaching certain concentration, the superiors' part can superpose levitating and be higher than the water surface, can dry up at last and dies, and some algae then is a sinking property, like chlorella, can form one and can not accept illumination, derive nutrients, growth effectively fully under water.The stirring of water wanted fully and slowly, and too fast meeting is pulled apart algal filament in a large number and is unfavorable for its growth.As leave the dead angle and still have the algae conglomeration and assemble the growth also be unfavorable for it.Heating also has special requirement, can not use common heating tube to heat, because the heating tube temperature distributing disproportionation is even, the algae that near the high temperature the heating tube can make is scalded dead at short notice.The mode work that should adopt whole even heating of incubator.In addition this type protect kind of incubator all the year round 24h work without cessation, energy-saving convenient, reliability and durability works long hours, and can effectively utilize the incubator volume, can cleaning for convenience detach all be the problem that will consider.
The utility model content
But the purpose of the utility model provides algae culture, guarantor's kind of special-purpose incubator of a kind of energy-saving convenient, reliability and durability, homogeneous heating, cleaning for convenience detach and life-time service.Its technical scheme is: incubator is made up of loam cake, transparent casing and base, and transparent casing internal upper part is equipped with light-emitting device, and blade is equipped with in the bottom, and the reducing motor of the outer correspondence position of casing is that the blade rotation provides power; Between transparent casing and the base heater body is installed.In last cover upper surface the oxygenation air pump being housed is that algae provides oxygen; Lower surface is equipped with singlechip controller; Singlechip controller is connecting the various sensor probes that are deep into incubator inside; Can monitor and control temperature, light intensity in the incubator, the dissolved oxygen of nutrient solution, pH value, transparency, electricity is led and multiple parameter such as ion content.The LED photodiode is equipped with as light source in the top of transparent casing; Blade is equipped with in the incubator bottom; The reducing motor of the outer correspondence position of the paddle wheel of paddle one side and incubator respectively is equipped with two pairs of magnet steel, sees through glass transmission power with magnetic force, drives blade and rotates; Make rotation in nutrient solution the uniform distribution of the interior algae of incubator owing to paddle, paddle is dismountable.Between base and the transparent casing heating system is housed; Heater body with the roughly the same heat conduction aluminium sheet tight riveting of transparent casing bottom glass shape; And the heat conduction aluminium sheet is bonded at transparent bottom half with heat-conducting silicone grease; Separated with the heat-protecting glass cellucotton between heater body and the base, heating system produces heat and makes the incubator even heating.
In order to make the algae in the incubator can obtain the needed sunlight of growing; Its casing adopts transparent glass to process, and under the room temperature condition with higher, takes away the outer stay-warm case of incubator; Sunlight just can make algae obtain good illumination condition through in top and the glassine cartonning all around.In order under the lower situation of room temperature, to heat for water tank, can't make algae dead because of local temperature is too high, adopt the bottom half gross area evenly to heat; Method is that toughened glass is adopted in the bottom; Toughened glass has good anti-mechanical impact and temperature resistance difference ability, and bottom glass can not make glass broken because of flexible difference that temperature head produced like this, and the method for even heating is with hot-plate and a thick aluminium sheet tight riveting roughly the same with the bottom glass shape; With heat-conducting silicone grease this piece aluminium sheet is bonded at the incubator bottom then; The heat that heating board is sent is distributed to aluminium sheet everywhere through the good aluminium sheet that conducts heat, and passes on the toughened glass of incubator bottom through heat-conducting silicone grease again, sees through toughened glass; Heat just is dispersed into water equably and has suffered; Water is under the effect of thermal convection and stirring runner, and the water in the whole box body is just heated equably, and the temperature difference is very little.Toughened glass and bonding between glass all around with acid Zylox, the binding property between them is fabulous, and can not come off medium-term and long-term immersion of water yet, and can tolerate 180 ℃ high temperature, be a kind of plan preferably.
For the water in the incubator can slowly mildly be moved, can too much not pull apart algal filament and have influence on algae grows, and make come-up and the algae that sinks at incubator uniform distribution can both be arranged; The utility model adopts sweep leaf Rotation Design at a slow speed, and blade grows to the both sides of incubator, and blade is pass on and moved the interior water of incubator and will move at leisure like this; Algae in the incubator is evenly distributed in water, can in incubator, can obtains good growth in order to make algae, the velocity of rotation of blade is an important factor; Rotating speed is too fast, is unfavorable for the growth of algae, and the kinetic energy of too slow current is not enough; There is not enough hydromechanics kinetic energy to make the algae uniform distribution; Algae is grown preferably, prove through protracted experience and orthogonal experiment, be 8cm at the paddle wheel diameter; When slurry length was 30cm, paddle wheel circular frequency was advisable at 25~36 commentaries on classics/min.Because paddle wheel is to work under water, when the power of paddle wheel transmitted, the dynamic seal of axle was very important; How to accomplish that motor will make runner rotation under water, prevent again to leak, also will dismantle easily; To be convenient to clean be the thing of difficulty, and the utility model has adopted the method for the easy magnetic field transferring power of ten minutes to solve problem dexterously, with the relative magnet steel of a pair of south poles is installed on the motor runner; Side at paddle wheel is also installed a pair of identical magnet steel, because glass does not have shielding effect to magnetic, magnetic force passes glass power is passed in the incubator; The transmission of power is separated by glass fully like this, any problem of leaking can not occur.The top of incubator is automatic control system, and operation such as transparency measurement is all accomplished by it in illumination, temperature, air pump oxygenation, the water.Power consumption is too big because incandescent light is worked all the year round; Do not meet the principle of environmental protection and saving, and the heat that incandescent light sends can influence the variation of water temperature, so abandoned this method; Luminescent lamp can not stepless dimming; Can only regulate light intensity with the method for opening many several, have big light intensity dead angle, not adopt so go out.The utility model adopts the White LED photodiode as light source, and a plurality of LED are combined by series and parallel, and by the direct supply driven for emitting lights, the duty through regulating power pulse is the step-less adjustment light intensity recently, can let light intensity be arranged on any one numerical value.Also can add transparency detector, photoconductive detector, pH value detector, different kinds of ions detector of dissolved oxygen detector in the water, hygrosensor, Light-Intensity Detector, water etc. in addition as required in addition.When the parameter in the water surpasses the upper lower limit value of setting, can control temperature adjustment by microcontroller system, operations such as air pump are started in light modulation, and can report to the police by the type of alarm that sets.Also can microcontroller system be imported desk-top computer or notebook computer through the mode of 485 serial communications, carry out more accurately and more complicated control by the numerical value of the various reference numbers of computer record and to environmental parameter.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the utility model is done further detailed explanation.
Fig. 1 is the utility model algae culture, the structural representation of protecting kind of special-purpose incubator.
Embodiment
Algae culture shown in Figure 1, guarantor's kind of special-purpose incubator are made up of loam cake 2, transparent casing 17 and base 10.At loam cake 2 upper surfaces oxygenation air pump 1 is housed and oxygen is provided for algae; Lower surface is equipped with singlechip controller 3; Singlechip controller 3 is connecting the various sensor probes 16 that are deep into incubator inside; Can monitor and control temperature, light intensity in the incubator, the dissolved oxygen of nutrient solution, pH value, transparency, electricity is led and multiple parameter such as ion content.LED photodiode 4 is equipped with as light source in the top of transparent casing; LED photodiode 4 by a plurality of when LED diode parallel connection or be composed in series; By the direct supply driven for emitting lights, the duty through regulating power pulse is the step-less adjustment light intensity recently, can let light intensity be arranged on any one numerical value; Blade 5 is equipped with in the bottom; Transmit power through the magnet steel of on paddle wheel 15 and reducing motor 12, installing 14 with magnetic force with the reducing motor 12 of the outer correspondence position of incubator; Driving blade 5 is that rotate in the axle center with adjustable rotating shaft 13, makes the algae uniform distribution in nutrient solution in the incubator.Paddle is dismountable.Between base 10 and the transparent casing 17 heating system is housed; Heater body 11 with roughly the same heat conduction aluminium sheet 7 tight rivetings of transparent casing 17 bottom glass shapes size; And heat conduction aluminium sheet 7 is bonded at transparent casing 17 bottoms with heat-conducting silicone grease 6; Separated with heat-protecting glass cellucotton 9 between heater body 11 and the base 10, heating system produces heat can make the incubator even heating.
Under the lower situation of room temperature, can when room temperature is higher, can remove stay-warm case at the incubator outer cover with stay-warm case, sunlight can make algae obtain good illumination condition through in top and the glassine cartonning all around.Toughened glass can be adopted in the bottom of transparent casing 17, makes bottom not rising and the situation such as break of Yin Wendu, and toughened glass is bonding with acid Zylox with transparent casing 17 glass all around.
For helping algae grows, the paddle wheel diameter is 8cm, and when slurry length was 30cm, paddle wheel circular frequency was advisable at 25~36 commentaries on classics/min.
Singlechip controller 3 can be imported desk-top computer or notebook computer through the mode of 485 serial communications, carries out more accurately and more complicated control by the numerical value of the various reference numbers of computer record and to environmental parameter.

Claims (8)

1. an algae culture, guarantor plant a special-purpose incubator; Form by loam cake (2), transparent casing (17) and base (10); It is characterized in that: transparent casing (17) internal upper part is equipped with light-emitting device (4); Blade (5) is equipped with in the bottom, and the reducing motor (12) of the outer correspondence position of casing provides power for blade rotates; Between transparent casing (17) and the base (10) heater body (11) is installed.
2. according to the described a kind of algae culture of claim 1, guarantor's kind of special-purpose incubator; It is characterized in that: heater body (11) with roughly the same heat conduction aluminium sheet (7) tight riveting of transparent casing (17) bottom tempering glass shape; And heat conduction aluminium sheet (7) is bonded at transparent casing (17) bottom with heat-conducting silicone grease (6), separated between heater body (11) and the base (10) with heat-protecting glass cellucotton (9).
3. according to the described a kind of algae culture of claim 1, guarantor's kind of special-purpose incubator, it is characterized in that: paddle wheel (15) and reducing motor (12) respectively are equipped with 2 magnet steel (14) at correspondence position.
4. according to the described a kind of algae culture of claim 1, guarantor's kind of special-purpose incubator, it is characterized in that: paddle is dismountable.
5. according to the described a kind of algae culture of claim 1, guarantor's kind of special-purpose incubator, it is characterized in that: light-emitting device (4) is made up of a plurality of LED luminotron serial or parallel connections.
6. according to the described a kind of algae culture of claim 1, guarantor's kind of special-purpose incubator; It is characterized in that: oxygenation air pump (1) is housed at loam cake (2) upper surface; Lower surface is equipped with singlechip controller (3), and singlechip controller (3) is connecting the sensor probe (16) that is deep into incubator inside.
According to any described a kind of algae culture in the claim 1 to 5, protect kind of a special-purpose incubator, it is characterized in that: at the incubator outer cover with stay-warm case.
8. according to the described a kind of algae culture of claim 6, guarantor's kind of special-purpose incubator, it is characterized in that: singlechip controller (3) is connected with desk-top computer or notebook computer through 485 serial modes.
CN2011202358304U 2011-07-06 2011-07-06 Dedicated incubator for algae cultivation and seed conservation Expired - Fee Related CN202116547U (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630494A (en) * 2012-04-25 2012-08-15 中国科学院武汉植物园 Cultural method and device for common nostoc
CN103449854A (en) * 2013-08-27 2013-12-18 浙江海洋学院 Outdoor fermentor for high-concentration marine biological bacterial fertilizer
CN107881088A (en) * 2017-12-07 2018-04-06 河西学院 A kind of artificial light source both culturing microalgae equipment
CN107927495A (en) * 2017-11-15 2018-04-20 王孔旭成 A kind of algae culture machine algae delivery of filtered reflux control system
CN108485913A (en) * 2018-04-03 2018-09-04 浙江大学 Double paddle wheel tablet photosynthetic reactors and microalgae Carbon fixation method
CN109679840A (en) * 2019-01-16 2019-04-26 上海海洋大学 Microalgae batch conservation culture apparatus and method by the device Batch Culture microalgae
WO2022147616A1 (en) * 2021-01-06 2022-07-14 Deane Arthur Apparatus and method for algae growth

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630494A (en) * 2012-04-25 2012-08-15 中国科学院武汉植物园 Cultural method and device for common nostoc
CN103449854A (en) * 2013-08-27 2013-12-18 浙江海洋学院 Outdoor fermentor for high-concentration marine biological bacterial fertilizer
CN103449854B (en) * 2013-08-27 2015-08-19 浙江海洋学院 Outdoor fermentor for high-concentration marine biological bacterial fertilizer
CN107927495A (en) * 2017-11-15 2018-04-20 王孔旭成 A kind of algae culture machine algae delivery of filtered reflux control system
CN107881088A (en) * 2017-12-07 2018-04-06 河西学院 A kind of artificial light source both culturing microalgae equipment
CN107881088B (en) * 2017-12-07 2020-10-16 河西学院 Artificial light source microalgae breeding equipment
CN108485913A (en) * 2018-04-03 2018-09-04 浙江大学 Double paddle wheel tablet photosynthetic reactors and microalgae Carbon fixation method
CN108485913B (en) * 2018-04-03 2021-08-06 浙江大学 Double-paddle wheel flat plate photosynthetic reactor and microalgae carbon sequestration method
CN109679840A (en) * 2019-01-16 2019-04-26 上海海洋大学 Microalgae batch conservation culture apparatus and method by the device Batch Culture microalgae
WO2022147616A1 (en) * 2021-01-06 2022-07-14 Deane Arthur Apparatus and method for algae growth

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Granted publication date: 20120118

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