CN202193783U - Microorganism cultivation system - Google Patents

Microorganism cultivation system Download PDF

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Publication number
CN202193783U
CN202193783U CN2011203163765U CN201120316376U CN202193783U CN 202193783 U CN202193783 U CN 202193783U CN 2011203163765 U CN2011203163765 U CN 2011203163765U CN 201120316376 U CN201120316376 U CN 201120316376U CN 202193783 U CN202193783 U CN 202193783U
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bioreactor
solar
microorganism cultivation
solar battery
battery apparatus
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张凯
朱振旗
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ENN Science and Technology Development Co Ltd
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ENN Science and Technology Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00Combinations of bioreactors or fermenters with other apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/59Biological synthesis; Biological purification

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  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The utility model discloses a microorganism cultivation system, which comprises an optical organism reactor and a solar battery device, wherein the optical organism reactor is used for cultivating microorganisms, and the solar battery device is used for regulating solar radiation reaching the optical organism reactor. According to the microorganism cultivation system which is provided with the solar battery device used for regulating solar radiation reaching the optical organism reactor, appropriate sunlight can be provided for cultivation of the microorganisms, and further growth of microalgaes is promoted, and biomass of the microalgaes is enhanced. Simultaneously, the temperature of the optical organism reactor can be decreased, so that the cost of temperature control of the optical organism reactor is reduced. Additionally, the solar battery is concurrently used for generating electricity, so that comprehensive utilization efficiency of solar energy can be enhanced.

Description

The microorganism cultivation system
Technical field
The utility model relates to the microorganism cultivation system, relates in particular to a kind ofly can improve the microorganism cultivation system that solar energy composite utilizes level.
Background technology
Bioreactor is the device that utilizes sunshine that the mikrobe with photosynthetic capacity is cultured.Little algae is as a kind of mikrobe; Can effectively utilize luminous energy, CO2 and inorganic salts synthetic protein, fat, glucide and multiple high added value biologically active substance, can cultivate through little algae and produce protective foods, foodstuff additive, feed, bio-feritlizer, makeup and other natural product.Therefore, utilize the little algae of bioreactor high-density breeding more and more general.
Photosynthetic condition mainly comprises intensity of illumination, temperature, CO2 concentration etc.Present micro algae culturing device can have been controlled the concentration of CO2 in the photosynthetic response gas well, and temperature controlling is relatively more difficult.In water surrounding, temperature is a very important ecological factor.Biological growth needs has certain TR, and in this TR, biology just can carry out vital movements such as its normal growth and breeding, is called comfort zone.When temperature variation exceeds comfort zone, biological vital movement will be affected, even dead.Particularly, the ecologist thinks that high temperature is even more serious to the vital movement influence of biology, causes biological death faster.For example: the comfort zone of micro algae growth is 8-32 degree centigrade, optimum temperature range 20-25 degree centigrade, in the time of 35 degrees centigrade, can in one day, keep recovering the ability of breeding, and can only keep about 2 hours for 38 degrees centigrade, just caused death in 30 minutes in the time of 40 degrees centigrade.Therefore, controlled temperature suitably, with improve mikrobe for example the living weight of little algae be a target of microorganism cultivation.
In order to control the temperature of both culturing microalgae, in the prior art, when sunshine, strong temperature was high; Usually adopt the method for lowering the temperature to bioreactor spray cold water; So consumed energy own, and labor intensive and water resources make the temperature controlled cost of bioreactor improve.
The utility model content
The utility model is devoted to solve above-mentioned the problems of the prior art, proposes a kind of microorganism cultivation system, and it can control the temperature of bioreactor at low cost, and can improve the solar energy composite utilising efficiency.
According to the utility model, a kind of microorganism cultivation system is proposed, comprising: bioreactor, in said bioreactor, culture mikrobe; Solar battery apparatus, said solar battery apparatus are provided for regulating the solar radiation that arrives bioreactor, and said solar battery apparatus is the form of solar panel, and said solar panel covers the top of said bioreactor.
According to an embodiment of the utility model, said solar panel comprises a plurality of solar panels that are arranged side by side.
According to an embodiment of the utility model, said microorganism cultivation system also comprises water receptacle, and said water receptacle is arranged on the surface of solar panel, is used to absorb the heat of solar radiation, gives birth to hot water thereby produce.
According to an embodiment of the utility model, said microorganism cultivation system also comprises store battery, and solar battery apparatus is connected to store battery, gets up with the electrical power storage that photovoltaic generation is produced.
According to an embodiment of the utility model, said microorganism cultivation system also comprises regenerative apparatus, is used for the hot water that the store water container produces.
An embodiment according to the utility model; Adjustable angle joint between solar panel and bioreactor; To regulate the solar radiation that arrives bioreactor, said solar battery apparatus is the form of hull cell, and said hull cell covers on the surface of bioreactor.
According to an embodiment of the utility model, said hull cell is the form that is arranged in lip-deep a plurality of strip cell of bioreactor by the certain distance compartment of terrain.
Based on each embodiment, can regulate the light transmittance 20-70% of solar battery apparatus through selecting solar battery apparatus.
The ratio of the spacing between the width of said strip cell and said strip cell can be preferably 1: 1 for 1: 0.1 to 1: 100.
According to the utility model, owing to utilize solar battery apparatus to regulate the solar radiation that arrives bioreactor, therefore, suitable illumination can be provided for the breed of mikrobe, to promote the growth of little algae, improve the living weight of little algae.Simultaneously,, therefore, can reduce the temperature of bioreactor, thereby reduce the temperature controlled cost of bioreactor because solar cell also plays the effect of sunshade; In addition, solar cell is also used as the generating purpose, can improve the efficient that solar energy composite utilizes.
For purpose, characteristic and the advantage that makes the utility model can be more obviously understandable, the utility model is described further below in conjunction with accompanying drawing and specific embodiment.
Description of drawings
Fig. 1 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of first embodiment of the utility model.
Fig. 2 A and 2B are the synoptic diagram according to the alternate embodiment of the layout of the solar cell of the microorganism cultivation system of Fig. 1.
Fig. 3 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of second embodiment of the utility model.
Fig. 4 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of the variation embodiment of second embodiment of the utility model.
Fig. 5 is the synoptic diagram of an example of microorganism cultivation system that adopts the battery arrangement of Fig. 1.
Embodiment
Below only pass through the embodiment of example shows the utility model.The utility model also can be implemented or use through other different mode, and each item details in this specification sheets can be carried out various adjustment and change under the situation of the general plotting that does not deviate from the utility model.Moreover accompanying drawing is only explained the basic conception of the utility model in a schematic way, thus not necessarily drafting in proportion of diagram, and only show the parts relevant in the diagram with the utility model, but obvious the utility model can comprise other parts according to practical application.In each view, identical Reference numeral is represented identical or similar parts.
Fig. 1 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of first embodiment of the utility model.Fig. 2 A-2B is the synoptic diagram according to the alternate embodiment of the layout of the solar cell of the microorganism cultivation system of Fig. 1.Shown in Fig. 1 and Fig. 2 A-2B, build above bioreactor 2 as ceiling with solar panel 1.In bioreactor 2, culture the for example mikrobe of little algae.Solar panel 1 is provided for regulating the solar radiation that arrives bioreactor 2.
Solar panel 1 shown in Figure 1 is straight plate shape, covers the top of flat bioreactor 2.Solar panel 1 shown in Fig. 2 A is the twisted plate shape, also covers the top of flat bioreactor 2.Though not shown, be appreciated that solar panel 1 also can have other shape, bioreactor 2 also can be the reactor drum of other form, for example tubular type or column reactor.In addition, the solar panel of building above bioreactor 1 can be for a plurality of solar panels side by side, shown in Fig. 2 B.Like this, when needing, can be through piling up a plurality of cell panels or, regulating the solar radiation that arrives bioreactor with the interval between the sliding door mode mobile battery plate.
Solar panel 1 as shown in Figure 1 can be the translucent solar cell plate.Through selecting battery material can obtain different light transmissions.According to an embodiment of the utility model, the translucent solar cell plate is processed by unijunction light-transmission type non-crystalline silicon, and transmittance is 20%~70%, is preferably 20%.Solar panel 1 also can adopt opaque material, in order to carrying out both culturing microalgae with the astigmatism around the bioreactor 2.
In addition, transmittance also can be regulated through the angle of regulating between solar panel 1 and the bioreactor 2.When hope increased transmittance, the sensitive surface that as far as possible makes solar panel and solar ray when hope reduces transmittance, made the sensitive surface of solar panel approaching vertical with solar ray near level as far as possible.About this point, can confirm based on geographical position of living in, locality, such as near the lower place of the latitude in equator, solar panel can with ground near vertical placement, and, can reduce angle of inclination with ground in the higher area of latitude.The angle of inclination is preferably in about 30 degree generally at 5~40 degree.In addition, the situation that can fall based on the sun liter of every day is regulated the angle of inclination of solar panel, realizes sensitive surface and sunshine level as far as possible.
The contriver finds, when intensity of solar radiation acquires a certain degree, particularly for the solar radiation of direct projection to bioreactor, can produce adverse influence to the growth of little algae.And utilize solar panel that the transmittance of solar radiation is dropped to 10%~70%; Particularly 20%; Thereby utilize a part of solar radiation and the bioreactor astigmatism on every side that see through solar panel to carry out both culturing microalgae; Suitable illumination can be provided for the breed of mikrobe,, improve the living weight of little algae to promote the growth of little algae.Simultaneously,, therefore, can reduce the temperature of bioreactor, thereby reduce the temperature controlled cost of bioreactor because solar cell also plays the effect of sunshade; In addition, solar cell is also used as the generating purpose, can improve the efficient that solar energy composite utilizes.
Fig. 3 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of second embodiment of the utility model.Solar battery apparatus shown in Figure 3 is the form of hull cell 1 ', and said hull cell 1 ' covers on the surface of bioreactor 2.Said hull cell 1 ' can be a unijunction light-transmission type amorphous silicon photovoltaic battery, also can be non-light-transmission type binode silicon-base thin-film battery.Identical with first embodiment shown in Fig. 1 and 2, can obtain different light transmissions through selecting battery material.For example select unijunction light-transmission type amorphous silicon photovoltaic battery, transmittance is 10%~70%, is preferably 20%.This embodiment can obtain the effect identical with the embodiment shown in Fig. 1 and 2.
Fig. 4 is the synoptic diagram according to the layout of the solar cell of the microorganism cultivation system of the change embodiment of second embodiment of the utility model.Hull cell as solar battery apparatus as shown in Figure 4 is the lip-deep a plurality of strip cell 1 that are arranged in bioreactor 2 by the certain distance compartment of terrain " form.Strip cell 1 " can be unijunction light-transmission type amorphous silicon photovoltaic battery; also can be non-light-transmission type binode silicon-base thin-film battery. institute's joint strip shape battery 1 " width and the ratio at the interval between the battery be 1: 0.1~1: 100; Preferred 1: 0.1~1: 10, preferably 1: 1.Equally, can be through piling up or the sliding door mode is controlled the width of strip cell and the ratio at the interval between the strip cell, thus the ratio of pasting battery part and expose portion on the surface of control photoreactor 2.Because cell is in the continuous motion; Sometimes by the battery partial occlusion, expose through expose portion sometimes, therefore; Through design strip cell 1 " width and the ratio at the interval between the battery; can be so that the cells in the bioreactor 2 be seen through time length of solar radiation irradiation of expose portion in the 1-100ms scope, and the time length of partly being covered by battery in the 10-1000 scope, promptly cell is illuminated long than being controlled at greater than in 0.1 the scope with the light and shade time of covering.Identical with first embodiment with second embodiment, can obtain different light transmissions through selecting battery material.
In addition; Present embodiment is through pressing certain distance arranged spaced solar cell on the bioreactor surface; Can the continuous light that shine on the bioreactor be transformed into discontinuous light; Thereby suitable illumination can be provided for the breed of mikrobe,, improve the living weight of little algae to promote the growth of little algae.Simultaneously,, therefore, can reduce the temperature of bioreactor, thereby reduce the temperature controlled cost of bioreactor because solar cell also plays the effect of sunshade; In addition, solar cell is also used as the generating purpose, can improve the efficient that solar energy composite utilizes.
Fig. 5 is the synoptic diagram of an example of microorganism cultivation system that adopts the battery arrangement of Fig. 1.As shown in Figure 5, the microorganism cultivation system also comprises water receptacle 3, and said water receptacle 3 is arranged on the surface of solar panel 1, is used to absorb the heat of solar radiation, thereby produces hot water.Said water receptacle 3 selects to see through the transparent material of sunshine.Said microorganism cultivation system also comprises store battery 4, and solar panel 1 is connected to store battery 4, and it is subsequent use to get up with the electrical power storage that photovoltaic generation is produced, and for example be that the source of artificial light of bioreactor 2 provides electric power night.Said microorganism cultivation system also comprises regenerative apparatus 5, be used for the hot water that store water container 3 produces, thereby store heat is subsequent use, and for example be that bioreactor 2 is heated night.
The advantage of the utility model has several respects:
1, solar cell and both culturing microalgae are combined together, improved light utilization efficiency.
2, the soil of same area, not only cultivating microalgae but also carry out solar electrical energy generation, conserve space improves land utilization ratio.
3, realized low-cost temperature control in the both culturing microalgae process, made reactor temperature can reach stable state, avoided the little algae survival of influence too high or too low for temperature and the speed of growth, the both culturing microalgae cycle is shortened.
4, because can low-cost controlled temperature, therefore, in office where manage position, any season all can cultivating microalgae, YO is improved.
Test
The contriver has carried out according to the microorganism cultivation system of the utility model and according to the test of the bioreactor culturing micro-organisms of prior art, and test conditions is following:
With chlorella (Chlorella) is object (other algae all can); Respectively in microorganism cultivation system like Fig. 5 as bioreactor and the utility model of the prior art of contrast; Adopt improvement SM substratum (medium component and content thereof see the following form 1-6); With equal densities inoculation logarithmic phase algae kind (change the training back formed in 5-7 days), initial OD 750 (cell quantity) is 0.2.Under natural lighting, cultivate this green alga, through aeration head bubbling air and CO2 mixed gas, gas-liquid volume ratio is 1: 16 from reactor bottom.Culture cycle is 6 days.
The composition and the content thereof of table 1. improvement SM substratum
# Composition Working concentration
1 Spir solution 1 500mL/L
2 Spir solution 2 500mL/L
Annotate: underscore is represented to be mixed with mother liquor in advance, and the preparation of said mother liquor sees table 2-3.
The composition and the content thereof of table 2.Spir solution 1
Figure BDA0000086375030000071
The composition and the content thereof of table 3.Spir solution 2
# Composition Working concentration Final concentration
1 NaNO 3 2.5g/500mL 36.2mM
2 K 2SO 4 1g/500mL 5.73mM
3 NaCl 1g/500mL 17.1mM
4 MgSO 4·7H 2O 0.2g/500mL 0.81mM
5 CaCl 2·2H 2O 0.04g/500mL 0.36mM
6 The P-IV metallic solution 6mL/0.5L
7 Chu micronutrient solution 1mL/0.5L
8 Vitamins B 12 1mL/0.5L
Annotate: underscore is represented to be mixed with mother liquor in advance, and the preparation of said mother liquor sees table 4-6.
The composition and the content thereof of table 4.P-IV metallic solution
# Composition Working concentration Final concentration
1 Na 2EDTA·2H 2O 0.75g/L 2mM
2 FeCl 3·6H 2O 0.097g/L 0.36mM
3 MnCl 2·4H 2O 0.041g/L 0.21mM
4 ZnCl 2 0.005g/L 0.037mM
5 CoCl 2·6H 2O 0.002g/L 0.0084mM
6 Na 2MoO 4·2H 2O 0.004g/L 0.017mM
The composition and the content thereof of table 5.Chu micronutrient solution
# Composition Working concentration Final concentration
1 CuSO 4·5H 2O 0.02g/L 0.78μM
2 ZnSO 4·7H 2O 0.044g/L 0.15μM
3 CoCl 2·6H 2O 0.02g/L 0.084μM
4 MnCl 2·4H 2O 0.012g/L 0.064μM
5 Na 2MoO 4·2H 2O 0.012g/L 0.052μM
6 H 3BO 3 0.62g/L 10μM
7 Na 2EDTA·2H 2O 0.05g/L 0.13μM
Table 6. vitamins B 12The composition of liquid storage and content thereof
Annotate: it should be appreciated by those skilled in the art that except as otherwise noted, the used reagent of the utility model all can be bought from common biological reagent company or chemical reagents corporation, its compound method also is that those skilled in the art is known.
Table 7. chlorella cultivation results (OD750)
Figure BDA0000086375030000092
Table 7 shows that chlorella is under with the temperature control method condition of the utility model and the growth result in the bioreactor under the sunshine direct projection condition.The result shows that this algae living weight under this cultivating system condition has improved about 1.8 times than control group living weight.
Though should be appreciated that and described the utility model with reference to embodiment and accompanying drawing, embodiment and accompanying drawing are not to be used to limit the utility model.Under the situation of the essence that does not depart from the utility model, any distortion that the utility model is done is all in the scope of the utility model.

Claims (11)

1. a microorganism cultivation system is characterized in that, comprising: bioreactor, in said bioreactor, culture mikrobe; Solar battery apparatus, said solar battery apparatus are provided for regulating the solar radiation that arrives bioreactor, and said solar battery apparatus is the form of solar panel, and said solar panel covers the top of said bioreactor.
2. microorganism cultivation according to claim 1 system is characterized in that said solar panel comprises a plurality of solar panels that are arranged side by side.
3. microorganism cultivation according to claim 1 and 2 system is characterized in that, the adjustable angle joint between solar panel and bioreactor is to regulate the solar radiation that arrives bioreactor.
4. microorganism cultivation according to claim 1 system is characterized in that, said microorganism cultivation system also comprises water receptacle, and said water receptacle is arranged on the surface of solar panel, is used to absorb the heat of solar radiation, gives birth to hot water thereby produce.
5. microorganism cultivation according to claim 1 system is characterized in that, said microorganism cultivation system also comprises store battery, and solar battery apparatus is connected to store battery, gets up with the electrical power storage that photovoltaic generation is produced.
6. microorganism cultivation according to claim 1 system is characterized in that, said microorganism cultivation system also comprises regenerative apparatus, is used for the hot water that the store water container produces.
7. a microorganism cultivation system is characterized in that, comprising: bioreactor, in said bioreactor, culture mikrobe; Solar battery apparatus, said solar battery apparatus are provided for regulating the solar radiation that arrives bioreactor, and said solar battery apparatus is the form of hull cell, and said hull cell covers on the surface of bioreactor.
8. microorganism cultivation according to claim 7 system is characterized in that said hull cell is the form that is arranged in lip-deep a plurality of strip cell of bioreactor by the certain distance compartment of terrain.
9. according to claim 1 or 7 described microorganism cultivation systems, it is characterized in that, is 20-70% through the transmittance of selecting solar battery apparatus to regulate solar battery apparatus.
10. microorganism cultivation according to claim 8 system is characterized in that the ratio of the spacing between the width of said strip cell and said strip cell is 1: 0.1 to 1: 100.
11. microorganism cultivation according to claim 8 system is characterized in that the ratio of the spacing between the width of said strip cell and said strip cell is 1: 1.
CN2011203163765U 2011-08-26 2011-08-26 Microorganism cultivation system Expired - Lifetime CN202193783U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105039138A (en) * 2015-08-19 2015-11-11 东台市赐百年生物工程有限公司 Microalgae culture system with solar cell panels and culture method thereof
CN105087374A (en) * 2015-08-19 2015-11-25 东台市赐百年生物工程有限公司 Carbon source supply system for spirulina cultivated in pool
CN105782880A (en) * 2016-03-02 2016-07-20 管天培 Solar charging and lamplight energy recovering type LED illuminating lamp

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105039138A (en) * 2015-08-19 2015-11-11 东台市赐百年生物工程有限公司 Microalgae culture system with solar cell panels and culture method thereof
CN105087374A (en) * 2015-08-19 2015-11-25 东台市赐百年生物工程有限公司 Carbon source supply system for spirulina cultivated in pool
CN105782880A (en) * 2016-03-02 2016-07-20 管天培 Solar charging and lamplight energy recovering type LED illuminating lamp

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