CN202322848U - Photosynthetic microorganism culture equipment - Google Patents

Photosynthetic microorganism culture equipment Download PDF

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Publication number
CN202322848U
CN202322848U CN 201120455587 CN201120455587U CN202322848U CN 202322848 U CN202322848 U CN 202322848U CN 201120455587 CN201120455587 CN 201120455587 CN 201120455587 U CN201120455587 U CN 201120455587U CN 202322848 U CN202322848 U CN 202322848U
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photosynthetic microorganism
temperature
culture vessel
culture
culture device
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CN 201120455587
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王明利
仵浩
王琳
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ENN Science and Technology Development Co Ltd
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ENN Science and Technology Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses photosynthetic microorganism culture equipment, which comprises a culture container, a centralized temperature control chamber, a centralized storage device and a pumping device, wherein the centralized storage device is arranged in the centralized temperature control chamber and is communicated with the culture container through a pipeline; when external environment temperature of the culture container exceeds a preset temperature range which is suitable for growth of photosynthetic microorganisms, photosynthetic microorganism culture solution in the culture container flows by the pipeline and flows into the centralized storage device; the pumping device is arranged on the pipeline; and when the external environment temperature of the culture container is in the preset temperature range which is suitable for growth of photosynthetic microorganisms, the photosynthetic microorganism culture solution stored in the centralized storage device is conveyed into the culture container by the pumping device. According to the photosynthetic microorganism culture equipment, the temperature control space is reduced and the energy is saved.

Description

The photosynthetic microorganism culture device
Technical field
The utility model relates to a kind of photosynthetic microorganism culture device.
Background technology
Little algae is one type of photosynthetic autotrophs unicellular organism that individuality is small, has extensive, of a great variety, the characteristics such as photosynthetic efficiency is high, fast growth, flexibility are strong that distribute.The annual fixed CO of little algae 2Account for 40% of global net photosynthesis output; Add it and be rich in high value natural pigments such as ester class, hydro carbons, albumen, soluble polysaccharide, astaxanthin, β-Hu Luobusu; Therefore in today that problems such as environmental protection, the energy and health attract tremendous attention, little algae more and more receives people's attention.
Micro-algae large-scale cultivation is restricted by factors such as temperature, illumination, environment cleanliness; Particularly temperature condition when the algae liquid temp is higher or lower than the suitable growth temperature range of little algae, will produce micro algae growth to press down to effect; When too high or too low for temperature, even cause little algae dead.In order to strengthen to temperature control in the micro-algae large-scale cultivation process; The modes of building the greenhouse that adopt can guarantee injecting of sufficient sunlight and clean environment more; Can realize controllability again to chamber environment temperature; Thereby the temperature of guaranteeing algae liquid in the greenhouse is in the suitable growth scope of little algae all the time, is easy to micro algae growth.
In traditional greenhouse temperature control method, mainly adopt blower fan, cascade summer; Mostly be gilled radiator or central air-conditioning winter, though temperature control is had certain effect, energy consumption is high, the high bottleneck that thereupon becomes restriction micro-algae large-scale cultivation and industrialized development of working cost.
Photosynthetic microorganism (little algae) large-scale cultivation technology is mainly utilized blower fan to lean on the air draft cooling, utilizes wet curtain to lean on the moisture evaporation cooling and is utilized the sunshade net by intercepting the solar radiation cooling summer in the existing known greenhouse; Generally utilize gilled radiator (and various forms of heating installation), central air-conditioning heating winter, or utilize modes such as covering and heat insulating quilt or employing hollow (heat insulation) material to be incubated.
A kind of greenhouse body and energy saving and environment friendly semiclosed algae bio-reactor that declines that is placed on the transparent vessel in the greenhouse of comprising disclosed among the Chinese patent No.CN200820005341.8.This reactor drum sun power capable of using, underground heat, industrial exhaust heat are the heat supply of greenhouse body, thereby are that the interior little algae of transparent vessel provides suitable growth temperature.
Above-mentioned variety of way is cultivated the mass-producing of photosynthetic microorganism (little algae) and has all been adopted the mode to culture entire space temperature control of living in, promptly to whole greenhouse temperature control.But greenhouse space is big, heat radiation/cooling area is big, and heat-conduction coefficient is big, and the required energy that provides is that most of energy all is converted into environment potential energy to the summation of greenhouse space and culture temperature control institute energy requirement, and the energy that supplies culture to utilize is less relatively.
Though utilized sun power, underground heat and industrial exhaust heat among the Chinese patent No.CN200820005341.8 in the disclosed technology,, reached the purpose of energy-conserving and environment-protective to avoid the use of traditional energies such as coal, combustion gas.But it is still to whole greenhouse temperature control; Still exist " greenhouse space is big, heat radiation/cooling area is big; heat-conduction coefficient is big, and the required energy that provides is that most of energy all is converted into environment potential energy to the summation of greenhouse space and culture temperature control institute energy requirement; the energy that supplies culture to utilize is less relatively " such problem, what just in this patent, waste is renewable energy source.
The utility model content
A purpose of the utility model provides a kind of energy-conservation photosynthetic microorganism culture device.
An aspect according to the utility model provides a kind of photosynthetic microorganism culture device, comprises culture vessel.Wherein, said photosynthetic microorganism culture device also comprises: concentrate temperature-controlling chamber; Be arranged on the pooled storage in the said concentrated temperature-controlling chamber; Said pooled storage is communicated with said culture vessel through pipeline; When the ambient temperature of culture vessel exceeded the predetermined temperature range of suitable photosynthetic microorganism growth, the photosynthetic microorganism nutrient solution in the culture vessel flow in the said pooled storage via said pipeline; With the pumping installation that is arranged on the said pipeline; When the ambient temperature of culture vessel was positioned at the predetermined temperature range of suitable photosynthetic microorganism growth, said pumping installation was transported to the photosynthetic microorganism nutrient solution of storing in the said pooled storage in the said culture vessel.
According to a preferred embodiment of the utility model, said culture vessel can be placed on the indoor indoor culture of carrying out, and also can be placed on the outdoor outdoor breeding that carries out.
According to another preferred embodiment of the utility model, said culture vessel can be placed in the greenhouse and culture, and the space in the said concentrated temperature-controlling chamber is far smaller than the space in the said greenhouse.
A preferred embodiment according to the utility model; The position of said culture vessel is higher than the position of said pooled storage; And said pipeline is provided with switch-valve; When switch-valve was opened, the photosynthetic microorganism nutrient solution in the said culture vessel can rely on deadweight to flow back into automatically in the said pooled storage via said pipeline.
According to another preferred embodiment of the utility model, the wall of said concentrated temperature-controlling chamber, floor and roof are provided with heat insulation/thermal insulation material.
According to another preferred embodiment of the utility model, be provided with temperature controlling system in the said concentrated temperature-controlling chamber.
According to another preferred embodiment of the utility model, said temperature controlling system comprises at least a in air-conditioning, earth source heat pump, industrial exhaust heat interchanger, heating installation, solar energy collector and the cascade.
According to another preferred embodiment of the utility model, said concentrated temperature-controlling chamber is arranged on underground in the said greenhouse.
According to another preferred embodiment of the utility model, the wall of the one side that faces south at least in said greenhouse or/and the roof partly or entirely be to process by light transmissive material.
According to another preferred embodiment of the utility model, said culture vessel is at least a in coiled container, flexible thin membrane type container, plate shaped container or the runway pond formula container.
Another preferred embodiment according to the utility model is provided with aerating apparatus in the said culture vessel, is used to stir said photosynthetic microorganism nutrient solution and supplies its growth required gas to said photosynthetic microorganism nutrient solution.
According to another preferred embodiment of the utility model, said pooled storage is processed by heat insulation/thermal insulation material.
Another preferred embodiment according to the utility model is provided with light compensating apparatus in the said culture vessel.
According to another preferred embodiment of the utility model, the said predetermined temperature range of suitable photosynthetic microorganism growth is that 15 degree are to 25 degree.
According to another preferred embodiment of the utility model, the temperature in the said concentrated temperature-controlling chamber is controlled between the lower value of predetermined temperature range of Schwellenwert and the growth of suitable photosynthetic microorganism of the temperature that photosynthetic microorganism can tolerate.
According to another preferred embodiment of the utility model, the Schwellenwert of the temperature that said photosynthetic microorganism can tolerate is 5 degree.
According to another preferred embodiment of the utility model, the temperature in the said concentrated temperature-controlling chamber is controlled at 10 degree.
In the utility model; When the temperature of warm indoor/outdoor exceeded the predetermined temperature range of suitable photosynthetic microorganism growth, the photosynthetic microorganism nutrient solution in the culture vessel flow in the pooled storage of concentrating temperature-controlling chamber, therefore via pipeline; Only need the less concentrated temperature-controlling chamber in internal space is carried out temperature control; And need not carry out temperature control to bigger whole greenhouse, internal space, and therefore, effective conserve energy.And; At some in particular cases; Even can not give and concentrate temperature-controlling chamber that any other heating or refrigeration equipment are provided; Only needing provide insulation with the thermal insulation material of concentrating temperature-controlling chamber, just can be with concentrating temperature in the temperature-controlling chamber to remain within the ultimate temperature that photosynthetic microorganism can tolerate, and any extra energy need be provided.
Description of drawings
Fig. 1 shows the photosynthetic microorganism culture device structure principle chart according to a preferred embodiment of the utility model.
Embodiment
Pass through embodiment below, and combine accompanying drawing, do further bright specifically the technical scheme of the utility model.In specification sheets, same or analogous drawing reference numeral is indicated same or analogous parts.Followingly the explanation of the utility model embodiment is intended to the overall utility model design of the utility model is made an explanation, and is not to be understood that and is a kind of restriction the utility model with reference to accompanying drawing.
Fig. 1 shows the photosynthetic microorganism culture device structure principle chart according to a preferred embodiment of the utility model.
Of Fig. 1, according to an embodiment of the utility model, the photosynthetic microorganism culture device mainly comprises greenhouse 1, is arranged on culture vessel 3, the concentrated temperature-controlling chamber 2 in the greenhouse 1 and is arranged on the pooled storage of concentrating in the temperature-controlling chamber 24.
As shown in Figure 1, concentrate the internal space of temperature-controlling chamber 2 must be far smaller than the internal space in greenhouse 1, this is in order need to reduce the space of temperature control, thereby can effectively reduce the waste of energy, the energy of saves valuable.In an embodiment of the utility model, the internal space ratio of the internal space in greenhouse 1 and concentrated temperature-controlling chamber 2 can be 125: 1.
Although in the embodiment shown in fig. 1, culture vessel 3 is placed on indoor culture,, need to prove that culture vessel 3 also can directly be placed on outdoor the breed.
As shown in Figure 1, pooled storage 4 is communicated with culture vessel 3 through pipeline 5.In a preferred embodiment of the utility model, the pipeline 5 that the pipeline 5 of pumping photosynthetic microorganism nutrient solution is provided with pumping installation 6 and backflow photosynthetic microorganism nutrient solution is provided with switch-valve 7.Simultaneously, in illustrated embodiment, the placement location of culture vessel 3 is higher than the position of pooled storage 4, and therefore, when switch-valve 7 was opened, the photosynthetic microorganism nutrient solution in the culture vessel 3 can rely on deadweight to flow back into automatically in the pooled storage 4 via pipeline 5.
As shown in Figure 1, when the temperature in greenhouse 1 exceeded the predetermined temperature range of suitable photosynthetic microorganism growth, switch-valve 7 was opened, and the photosynthetic microorganism nutrient solution in the culture vessel 3 flow back in the pooled storage 4 via pipeline 5.When the temperature in greenhouse 1 was positioned at the predetermined temperature range of suitable photosynthetic microorganism growth, switch-valve 7 cut out, and pumping installation 6 starts, and was used for the photosynthetic microorganism nutrient solution of pooled storage 4 storages is transported in the culture vessel 3.
In an embodiment of the utility model, in order to guarantee to concentrate temperature-controlling chamber 2 to have good heat-proof quality ability, wall, floor and the roof of concentrated temperature-controlling chamber 2 can be provided with heat insulation/thermal insulation material or directly processed by heat insulation/thermal insulation material, like foam, plastics etc.Like this, under some normal circumstancess, can only rely on the heat-insulating property of concentrating temperature-controlling chamber's 2 materials self, just can will concentrate temperature in the temperature-controlling chamber 2 to be controlled in the temperature range limit that photosynthetic microorganism can tolerate, for example 5 degree are to 40 degree, thereby realize zero energy consumption.But under some extreme environment, for example outside temperature is extremely crossed low or too high; At this moment; Just need in concentrating temperature-controlling chamber 2, increase extra temperature controlling system, for example, at least a in central air-conditioning, earth source heat pump, industrial exhaust heat interchanger, heating installation, solar energy collector and the cascade.Like this, regardless of external environment, always can perhaps be controlled at a constant temp with concentrating the temperature in the temperature-controlling chamber 2 to be controlled in the reasonable range.
In order further to improve insulation and control thermal property, in an embodiment of the utility model, pooled storage 4 is processed by thermal insulation/lagging material, for example, and pottery etc.
In addition, in a preferred embodiment of the utility model, concentrate temperature-controlling chamber 2 to be arranged on underground in the greenhouse 1, so also can further improve the insulation of concentrating temperature-controlling chamber 2 and control thermal property.But, note that the utility model is not limited to this embodiment, concentrate temperature-controlling chamber 2 also can be arranged on the ground or be arranged on the outside in greenhouse 1.
In order to have guaranteed that sufficient sunlight injects in the greenhouse 1, in an embodiment of the utility model, the wall of the one side that faces south at least in greenhouse 1 or/and the roof partly or entirely be to process by light transmissive material, for example, glass or plastics.
In an embodiment of the utility model, what culture vessel 3 can be in coiled container, flexible thin membrane type container, plate shaped container or the runway pond formula container is at least a.
In another embodiment of the utility model, be provided with light compensating apparatus in the culture vessel 3, for example the LED lamp is used for when extraneous sunlight is not enough, and the photosynthetic microorganism in culture vessel 3 replenishes light, promotes its growth.
In another embodiment of the utility model, can be provided with aerating apparatus in the culture vessel 3, be used to stir the photosynthetic microorganism nutrient solution and supply its growth required gas to the photosynthetic microorganism nutrient solution, for example, CO 2Gas.
To combine a concrete application example to specify the utility model below.
[instance]
This instance is primarily aimed in northern greenhouse mass-producing both culturing microalgae.Greenhouse 1 adopts the glass heliogreenhouse, and the internal space for example can be 500m 3, culture vessel 3 for example can be the tubular type bioreactor, its cubic capacity can be 2m 3, culture is that little algae, total internal space of concentrating temperature-controlling chamber 2 can be 4m 3, be lined with warming plate all around.Pooled storage 4 can be 2.5m 3Sugared porcelain storage tank.Little algae optimum growth temperature can be 15 ℃-25 ℃, and tolerable temperature can be 5 ℃-40 ℃, with one month November culture experiment be example.
Daytime the 9-10 point, temperature can reach about 15 ℃ in the glasshouse, this moment, the algae liquid that is stored in the pooled storage 4 was delivered in the tubular type bioreactor 3 via pipeline 5 with pump 6, in greenhouse 1, carried out photosynthesis; Afternoon, 4-5 o'clock greenhouse 1 interior temperature was lower than 15 ℃, opened valve 7, made algae liquid under action of gravity, 5 flow to voluntarily in the pooled storage 4 by the road.Concentrate temperature-controlling chamber 2 heat to be provided, the algae liquid temp is controlled at about 10 ℃ by earth source heat pump.After temperature rises to the optimal temperature of micro algae growth in the next day 9-10 point glasshouse 1, again the algae liquid pump in the pooled storage 4 is gone in the bioreactor 3 in the greenhouse 1 to culture.
Compare with control group (adopt the heat pump air conditioner heating, temperature maintains about 20 ℃ all the time), central heating mode energy consumption is 0.5% of a control group, and output improves 7%.
Cultivating microalgae is concentrated in the mass-producing in winter; Reduced the temperature control space greatly, reduced area of dissipation, reduced heat-conduction coefficient with respect to prior art; Reduced the conversion of heating-amount, reduced heating energy consumption, thereby a nearly step is reduced the aquaculture cost of little algae to environment potential energy direction; On the other hand, but dark, low temperature, high dissolved oxygen environment have little algae respiration to effect, help the accumulation of culture living weight.
Compared with prior art, the utility model has the following advantages at least:
1, the utility model comprises a concentrated temperature-controlling chamber, is positioned at the underground of greenhouse, and being used in the greenhouse when outside temperature is lower than algae liquid suitable growth temperature is that algae liquid is concentrated insulation or temperature control.
2, comprise pooled storage, be positioned at and concentrate temperature-controlling chamber, be used for the algae liquid of centralized stores insulation or temperature control process.
3, the present invention avoids the energy input to culture environment of living in culture collection central heating and refrigeration together, and the utilization of energy is more effective.
4, together, place dark, low temperature, high dissolved oxygen environment effectively to press down, increase the culture biomass accumulation to the culture respiration with culture collection.
Though in conjunction with accompanying drawing the utility model is illustrated, disclosed embodiment is intended to the utility model preferred implementation is carried out exemplary illustration in the accompanying drawing, and can not be interpreted as a kind of restriction to the utility model.
Though some embodiment of this overall utility model design are shown and explain; Those skilled in the art will appreciate that; Under the situation of principle that does not deviate from this overall utility model design and spirit; Can make a change these embodiment, the scope of the utility model limits with claim and their equivalent.

Claims (12)

1. a photosynthetic microorganism culture device comprises culture vessel (3),
It is characterized in that said photosynthetic microorganism culture device also comprises:
Concentrate temperature-controlling chamber (2);
Be arranged on the pooled storage (4) in the said concentrated temperature-controlling chamber (2); Said pooled storage (4) is communicated with said culture vessel (3) through pipeline (5); When the ambient temperature of culture vessel (3) exceeded the predetermined temperature range of suitable photosynthetic microorganism growth, the photosynthetic microorganism nutrient solution in the culture vessel (3) flow in the said pooled storage (4) via said pipeline (5); With
Be arranged on the pumping installation (6) on the said pipeline (5); When the ambient temperature of culture vessel (3) was positioned at the predetermined temperature range of suitable photosynthetic microorganism growth, said pumping installation (6) was transported to the photosynthetic microorganism nutrient solution of storage in the said pooled storage (4) in the said culture vessel (3).
2. photosynthetic microorganism culture device according to claim 1 is characterized in that: said culture vessel (3) is placed on indoor or outdoors.
3. photosynthetic microorganism culture device according to claim 2 is characterized in that: said culture vessel (3) is placed in the greenhouse (1), and the space in the said concentrated temperature-controlling chamber (2) is less than the space in said greenhouse (1).
4. photosynthetic microorganism culture device according to claim 3 is characterized in that:
The position of said culture vessel (3) is higher than the position of said pooled storage (4), and
Said pipeline (5) is provided with switch-valve (7), and when switch-valve (7) when opening, the photosynthetic microorganism nutrient solution in the said culture vessel (3) can rely on deadweight to flow back into automatically in the said pooled storage (4) via said pipeline (5).
5. photosynthetic microorganism culture device according to claim 3 is characterized in that: wall, floor and the roof of said concentrated temperature-controlling chamber (2) are provided with heat insulation/thermal insulation material.
6. photosynthetic microorganism culture device according to claim 5 is characterized in that: be provided with temperature controlling system in the said concentrated temperature-controlling chamber (2).
7. photosynthetic microorganism culture device according to claim 6 is characterized in that: said temperature controlling system comprises at least a in air-conditioning, earth source heat pump, industrial exhaust heat interchanger, heating installation, solar energy collector and the cascade.
8. photosynthetic microorganism culture device according to claim 3 is characterized in that: said concentrated temperature-controlling chamber (2) is arranged on underground in the said greenhouse (1).
9. photosynthetic microorganism culture device according to claim 3 is characterized in that: the wall of the one side that faces south at least in said greenhouse or/and the roof partly or entirely be to process by light transmissive material.
10. photosynthetic microorganism culture device according to claim 3 is characterized in that: said culture vessel (3) is at least a in coiled container, flexible thin membrane type container, plate shaped container or the runway pond formula container.
11. photosynthetic microorganism culture device according to claim 3 is characterized in that: said culture vessel is provided with aerating apparatus in (3), is used to stir said photosynthetic microorganism nutrient solution and supplies its growth required gas to said photosynthetic microorganism nutrient solution.
12. photosynthetic microorganism culture device according to claim 3 is characterized in that: said pooled storage (4) is processed by heat insulation/thermal insulation material.
CN 201120455587 2011-11-16 2011-11-16 Photosynthetic microorganism culture equipment Expired - Lifetime CN202322848U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783385A (en) * 2012-08-17 2012-11-21 沈阳农业大学 Water curtain system of solar greenhouse
CN105087374A (en) * 2015-08-19 2015-11-25 东台市赐百年生物工程有限公司 Carbon source supply system for spirulina cultivated in pool

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783385A (en) * 2012-08-17 2012-11-21 沈阳农业大学 Water curtain system of solar greenhouse
CN105087374A (en) * 2015-08-19 2015-11-25 东台市赐百年生物工程有限公司 Carbon source supply system for spirulina cultivated in pool

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