CN102539366B - Device for detecting swinging bed fermentation liquid by double light sources - Google Patents

Device for detecting swinging bed fermentation liquid by double light sources Download PDF

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Publication number
CN102539366B
CN102539366B CN 201110449182 CN201110449182A CN102539366B CN 102539366 B CN102539366 B CN 102539366B CN 201110449182 CN201110449182 CN 201110449182 CN 201110449182 A CN201110449182 A CN 201110449182A CN 102539366 B CN102539366 B CN 102539366B
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light
triangular flask
fermentation
liquid
silica gel
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CN102539366A (en
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刘志强
沈水兴
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SHANGHAI ZHICHENG ANAIYTICAL INSTRUMENT MANUFACTURING Co Ltd
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SHANGHAI ZHICHENG ANAIYTICAL INSTRUMENT MANUFACTURING Co Ltd
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Abstract

The invention provides a device for detecting swinging bed fermentation liquid by double light sources. The device comprises swinging bed equipment, a liquid fermentation triangular bottle and a glass triangular bottle, wherein the liquid fermentation triangular bottle is arranged on a swinging bed, the glass triangular bottle contains fermentation liquid, is coated with a black coating and is fixedly arranged on a silica gel frame, the silica gel frame is fixedly arranged on a swinging bed tray, the inner side of the silica gel frame is provided with two light emitting electrodes and corresponding light receiving electrodes, the two light emitting electrodes mutually form an angle being 90 degrees, in addition, the light emitting electrodes and the light receiving electrodes are anastomotic with the positions of light transmission holes arranged on the triangular bottle containing fermentation liquid in the middle, simultaneously, the inner side of the silica gel frame at the other end is also provided with two light emitting electrodes and corresponding light receiving electrodes, the two light emitting electrodes mutually form an angle being 90 degrees, in addition, the light emitting electrodes and the light receiving electrodes are anastomotic with the other two through holes arranged on the triangular bottle containing the fermentation liquid in the middle, received light signals are converted into electric signals by the light receiving electrodes, the fermentation liquid concentration is increased along with the gradual increase of the biological thallus quantity in the triangular body, the transmitted light is changed, and the electric signals of the light receiving electrodes are also changed, so the growth degree of the fermentation thalli in the triangular bottle is judged. Through the double light sources, the detection sensitivity can be improved, and simultaneously, the device is also suitable for being used for detecting the work states of various kinds of fermentation liquid in industry.

Description

The device of two light source detection shaker fermentation liquid
Technical field
The present invention relates to a kind of device of tracer liquid sweat.
Technical background
Detect at present the fermented liquid on shaking table, be all to see through the Erlenmeyer flask range estimation directly to observe or take out fermented liquid and carry out the thalline counting, or filter centrifugal, take the weight of solid content, determine the means such as biomass, the analytic process complexity, time is long, and error is larger.
content of the present invention
Analyze in order to solve above-mentioned fermentation the problem existed, the invention provides the device of a kind of pair of light source detection shaker fermentation liquid, comprise shaking table equipment, be placed in the liquid fermentation triangular flask on shaking table, fill fermented liquid and scribble the Erlenmeyer flask of black coating, be fixed on the silica gel frame, the silica gel frame is fixed on the shaking table pallet, silica gel frame inboard arranges two and is in the light emitting electrode of an angle of 90 degrees and corresponding light-receiving electrode, and match with the light hole position that be equipped with on the triangular flask of fermented liquid centre, other end silica gel frame inboard also arranges two and is in the light emitting electrode of an angle of 90 degrees and corresponding light-receiving electrode simultaneously, and match with another two open-works that be equipped with on the triangular flask of fermented liquid centre, the light-receiving electrode is converted to electric signal by the light signal of reception, with the biological bacteria scale of construction in triangular flask, increase gradually, fermented liquid concentration increases, and the light seen through is changed, the electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in triangular flask.
Described triangular flask, except being mutually four light holes of an angle of 90 degrees, and, outside the triangular flask bottom, all the other all scribble the black light screening material, and light hole is all below the fermentation liquid level.
Described two ultraviolet rays that transmitting illuminant is infrared ray or selected wavelength, two utilizing emitted lights are identical wavelength or different wave lengths.
Described two light-receiving electrodes are connected with photoelectric sensor, and the light signal of reception is converted to electric signal, by being wirelessly transmitted to display screen, or mobile phone, or on media player, the extent of growth of fermentation liquor thalline in instant playback shaking table triangular flask.
Advantage of the present invention is: 1) adopt photoelectricity as detection means, photoelectricity is propagated distance, can reach several km, and minimum for very-short-reach propagated error in bottle.2) two light sources can detect two samples in fermented liquid simultaneously, can distinguish solution and colloid, ultraviolet light wave as infrared light and can confirm the group molecule, and thalline increases can change strength of fluid, light absorption value is had a direct impact, therefore two light sources are surveyed fermented liquid, highly sensitive, easily apply.3) utilize the solid neutral colour filter to obtain light source, method is simple, economy and durability, and technology maturation, be easy to realize suitability for industrialized production.4) compare with the traditional glass Erlenmeyer flask, adopt the shading triangular flask, many advantages are arranged: at first the black coating light-proofness is good, can avoid diffracted ray to disturb, and guarantees the Photoelectric work precision; Secondly, chrome-plated material shading triangular flask conduction is good, and the temperature that can effectively utilize shaking table to provide also provides advantage for exploitation bottom alternating temperature shaking table pallet; 5) the silica gel fixed mount is flexible, sturdy and durable, prevents the triangular flask displacement and comes off, and can also place in inside light emission and light absorption electric elements.6) device of two light source detection shaker fermentation liquid, can draw the photoelectric absorption curve by revision test, and according to the curve programming, the Based Intelligent Control shaking table, repeat the stages that ferments, and for industrial bulk fermentation provides, operates accurately production routine.7) do not need sampling detect can by display screen and (or) mobile phone, (or) multimedia etc. shows fermentation liquor terms and conditions and relevant information, particularly can provide the shaker fermentation orthogonal, accurate experiment parameter is provided, improve scientific research efficiency and experimental precision.
The accompanying drawing explanation
Fig. 1 is the two light source detection shaker fermentation liquid mounting structure figure of the present invention;
The two light source triangular flask structural drawing of Fig. 2.
code name explanation in figure
1, triangle shaking flask; 2, light hole;
3, silica gel frame; 4,7, emitting electrode;
5,9 receiving electrodes (9 4 the back side); 8,10 photoelectric sensors (10 4 the back side);
6, shaking table pallet, 11, shading coating; 12,13,14,15 light holes (15 14 the back side).
Embodiment
The present invention includes shaking table equipment, be placed in the liquid fermentation triangular flask 1 on shaking table, fill fermented liquid and scribble the Erlenmeyer flask of black coating, be fixed on silica gel frame 3, silica gel frame 3 is fixed on shaking table pallet 6, silica gel frame 3 inboards arrange two the light emitting electrode 4 and the light-receiving electrodes 5 that are in an angle of 90 degrees, and with centre, the light hole 14 on the triangular flask 1 of fermented liquid is housed, 12 positions match, simultaneously other end silica gel frame 3 inboards also arrange two light emitting electrodes 7 that are in an angle of 90 degrees and light-receiving electrode 9(9 at 4 the back side), and with centre, another two open-works 13 on the triangular flask of fermented liquid are housed, 15 match, light-receiving electrode 5,9 is converted to electric signal by the light signal of reception, with the biological bacteria scale of construction in triangular flask, increase gradually, fermented liquid concentration increases, and the light seen through is changed, the electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in triangular flask.
Described triangular flask, except being mutually four light holes 12,13,14,15 of an angle of 90 degrees, and, outside the triangular flask bottom, all the other all scribble black light screening material 11, and light hole is all below the fermentation liquid level.
Described two ultraviolet rays that transmitting illuminant is infrared ray or selected wavelength, two utilizing emitted lights are identical wavelength or different wave lengths.
Described two light-receiving electrodes 5,9 are connected with photoelectric sensor 8,10, and the light signal of reception is converted to electric signal, by being wirelessly transmitted to display screen, or mobile phone, or on media player, the extent of growth of fermentation liquor thalline in instant playback shaking table triangular flask.
Embodiment 1:
bacterial classification: get three kinds of different lactic acid bacteria culturers, L 1, L 2and L 3, making respectively bacteria containing amount is 10 5 ~ 6individual/ml suspension bacteria liquid is standby; sterilizing: get 250ml shading triangular flask, after numbering, respectively add beef broth potato nutrient solution 100ml<± 0.1%(gets 10% beef broth nutrient solution 20ml and adds in PDA potato nutrient solution), after sterilizing is cooling, be that the nutritious fermentation liquor body is standby; inoculation:measure the bacteria suspension 5ml of different strain with pipettor, add in the shading triangular flask that contains the nutritious fermentation liquor body, above each bacterial classification fills three bottles (being triplicate), and only a fermented liquid triangular flask does not add bacterial classification, for contrast, above totally 10 bottles; install: the shading triangular flask is fixed on the silica gel frame, screws turn-knob, optoelectronic pole and triangular flask light hole are matched.Open photoelectricity power switch on control panel, make on the silica gel frame light of two light source luminescent electrodes, see through the triangular flask nutrient liquid, receive to the light-receiving electrode of opposite side; the start operation: open shaking table control panel photoelectricity master switch, by fine setting, the light absorption number of all triangular flasks is put and be zero; Adjusting the shaking table temperature is 32 ℃, and revolution is 250 rev/mins, starts shaking table; observe: measured an absorbance value every 30 minutes, until absorbance value only occurs when on a declining curve behind peak.Relatively two light source acquired results, take the time as horizontal ordinate, take absorbance value as ordinate, draws absorption curves, when the light absorption peak value of take is the highest, is the best fermentation time of this mushroom type; With light absorption peak value the maximum, it is best dominant strain.More than can be the basic operation standard of Intelligent Measurement shaker fermentation liquid bio quality.
Embodiment 2:
Get the advantage lactic acid bacteria strains in embodiment 1, making bacteria containing amount is 10 5 ~ 6individual/ml suspension bacteria liquid is standby; Sterilizing, inoculation, installation and start operation are with embodiment 1; Wherein two optical source wavelengths 1 are set as infrared light, detect the fermented liquid colloid concentration; Wavelength 2 is set as ultraviolet light, detects the glucose residual volume in fermented liquid, and initial light absorption value is made as zero, and with the growth of fermentation thalline, glucose content descends, and wavelength 2 light absorption values can present negative value; Detected absorbance, sample analysis thalline content, glucose residual after shaking table work every 30 minutes, according to analysis result, suitably add nutrient solution and adjust ph, data are stored, repeated experiments is three times continuously, averages, and take the time as horizontal ordinate, take absorbance, thalline content and glucose residual is figure as ordinate, draws the two light source light electro-detection shaking table working curves of this bacterial strain.The working curve using method is: the nutrient formulation provided by this shaking table, bottled, sterilizing, cooling inoculation, Installation and Debugging, setting wavelength 1 and wavelength 2, started shooting etc., absorbance value and the corresponding typical curve result of absorbance value in the close observation fermentation time, numerical value is close shows that fermentation system is working properly, and press the typical curve operation indicating, add in time nutrient solution and adjust ph, until finish, finally obtaining desirable fermentation results; If online numerical value and typical curve differ greatly, can also point out may occur abnormal by automatic alarm, shutdown inspection, avoid loss in case of necessity.
Two electrodes of two light source light electro-detection shaking tables can send the light of identical wavelength or different wave length, are conducive to detect detected materials two or more in fermentation liquor simultaneously, as the colloid concentration in fermented liquid or certain biopreparate; With the thalline incubation time, extend, changing appears in bacterial concentration, and bacterial concentration is high, shows that thalline content is many, to the absorbance increase, is directly proportional; By observing the variation of two light source absorbances, be conducive to analyze more accurately, faster fermentation condition to the impact of fermented liquid microbial activity and the product quality of definite fermented liquid, no longer need sampling to detect.Two light source light electro-detection shaker fermentation liquid, triangular flask used is except adopting the black shading film and have the sensitization position, and other and common triangular flask are identical; The course of work of two light source detection shaker fermentation liquid is: 1) prepare liquid spawn: according to the different experiments needs, prepare liquid spawn, prepare suspension bacteria liquid standby; 2) preparation nutritious fermentation liquor body: according to the different strain growth needs, accurately prepare the nutrient matrix of suitable growth; While using 250ml shading triangular flask, add nutrient liquid 100 and (or) 120ml, loading amount error<± 0.1%; 3) sterilizing: the shading triangular flask of be added with nutrient liquid is carried out to high-temp steam sterilizing, and in 121 ℃, pressure 0.15Mpa keeps 15 minutes, while being cooled to 23 ~ 26 ℃, can access bacterial classification; 4) inoculation: bacterium liquid suspension concentration is controlled at 10 6 ~ 7individual/ml, with pipettor accurately add 5 and (or) 10ml bacterium liquid/every bottle; While inoculating thread thalline, use 0.5cm 2card punch get mycoderm 5 and (or) 10 slices, in the shading triangular flask that access contains the nutritious fermentation liquor body; During inoculation wherein the nutrient liquid of at least one triangular flask do not add bacterial classification, be contrast; 5) start debugging: open general supply, adjust electroluminescent lamp, the light source that electroluminescent lamp is sent sees through the triangular flask nutrient liquid, by triangular flask opposite side light-receiving electrode, is received, and changes electric signal into, then processes and obtain obvious numerical signal through the multiplication amplifying circuit; 6) wavelength set of two optoelectronic poles: detect sample according to difference and need to set the light that two optoelectronic poles send identical wavelength or different wave length, as the concentration of measuring thalline in fermented liquid and the concentration of glucose remnants simultaneously, can set respectively that an electrode light source sends the 100nm infrared wavelength and another sends the 260nm ultraviolet wavelength; For meeting L 9(3) 4orthogonal test needs, and usually take 10 as one group, can detect three different samples, and wherein 9 are accessed bacterial classifications, and 1 does not connect bacterial classification in contrast.Usually triplicate, average and do regretional analysis.The silica gel fixed mount is anchored on the shaking table pallet, and light emitting electrode, light absorption photoelectric commutator be placed in the silica gel frame, with wireless transmit, and by display screen, and (or) mobile phone, and (or) demonstration such as multimedia.Two light source detection shaking table liquid fermentation principle of work: fermented liquid will be housed and access the shading triangular flask of bacterial classification, and be fixed on shaking table silica gel frame, the frame turn-knob that is tightened, make two light emitting electrodes and two light-receiving electrodes and four light holes of triangular flask automatically identical, opening optoelectronic switch makes two emitting electrodes all luminous, and send specific light wave by optical filter, see through nutrient liquid until the triangular flask opposite side, by two light-receiving electrodes, received, initial by the fine setting turn-knob, photoelectricity is received to the display number and put that all to be adjusted to be zero, set revolution (150 ~ 160 rev/mins), start shaking table after temperature (25 ~ 28 ℃), with shaking table work time lengthening, the fermented liquid concentration change, changing appears in the numerical value on the light inductance receiver, until numerical value is when constant, at this moment numerical value is large, in corresponding triangular flask, thalline content is maximum, the also significant change of biochemical metabolism index, and contrast triangular flask numerical value is still zero.Thus, can determine the product quality of shaker fermentation liquid.

Claims (4)

1. the device of two light source detection shaker fermentation liquid, comprise shaking table, be placed in the liquid fermentation triangular flask on shaking table, it is characterized in that: described triangular flask is the Erlenmeyer flask that fills fermented liquid and scribble black coating, be fixed on the silica gel frame, the silica gel frame is fixed on the shaking table pallet, silica gel frame inboard arranges two and is in the light emitting electrode of an angle of 90 degrees and corresponding light-receiving electrode, and match with the light hole position that be equipped with on the triangular flask of fermented liquid centre, other end silica gel frame inboard also arranges two and is in the light emitting electrode of an angle of 90 degrees and corresponding light-receiving electrode simultaneously, and match with another two light holes that be equipped with on the triangular flask of fermented liquid centre, the light-receiving electrode is converted to electric signal by the light signal of reception, with the biological bacteria scale of construction in triangular flask, increase gradually, fermented liquid concentration increases, and the light seen through is changed, the electric signal of light-receiving electrode also changes thereupon, thereby judges the extent of growth of fermentation liquor thalline in triangular flask.
2. the device of according to claim 1 pair of light source detection shaker fermentation liquid, is characterized in that, described triangular flask, except being mutually four light holes of an angle of 90 degrees, and, outside the triangular flask bottom, all the other all scribble the black light screening material, light hole is all below fermentation liquor dignity.
3. the device of according to claim 1 pair of light source detection shaker fermentation liquid, is characterized in that, the ultraviolet ray that the light of described smooth emitting electrode emission is infrared ray or selected wavelength, and two utilizing emitted lights are identical wavelength or different wave lengths.
4. the device of according to claim 1 pair of light source detection shaker fermentation liquid, it is characterized in that, described two light-receiving electrodes are connected with photoelectric sensor, the light signal of reception is converted to electric signal, by being wirelessly transmitted to display screen, or mobile phone, or on media player, the extent of growth of fermentation liquor thalline in instant playback shaking table triangular flask.
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CN103323433B (en) * 2013-06-20 2016-08-10 上海智城分析仪器制造有限公司 The shaking table device of contactless multichannel on-line checking fermentation liquid and method of testing

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CN102519898A (en) * 2011-12-29 2012-06-27 上海智城分析仪器制造有限公司 Device utilizing single light source to detect fermentation liquid of shake table

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