CN103063639A - Microbial growth optical detection sensor - Google Patents
Microbial growth optical detection sensor Download PDFInfo
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- CN103063639A CN103063639A CN2012105834231A CN201210583423A CN103063639A CN 103063639 A CN103063639 A CN 103063639A CN 2012105834231 A CN2012105834231 A CN 2012105834231A CN 201210583423 A CN201210583423 A CN 201210583423A CN 103063639 A CN103063639 A CN 103063639A
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Abstract
The invention relates to a medical detection apparatus and especially relates to a microbial growth optical detection sensor. The microbial growth optical detection sensor comprises a light emitting diode array (401) composed of four LEDs, an optical filter (402), a photodiode (403), a hollow cylindrical base (404), and a light-emitting diode constant current power supply (405), wherein the optical filter (402) is a vacuum-coated optical filter. The microbial growth optical detection sensor utilizes fluorescence and chromometry principles of microbial growth, realizes high-sensitivity accurate detection of microbial growth, and realizes full automation of blood specimen bacterium growth and detection.
Description
Technical field
The present invention relates to medical instruments and equipment, in particular to a kind of growth of microorganism optical detecting sensor for Automated blood culture instrument detecting system.
Background technology
Because the progress of medical skill, various diagnoses and treatment means grow with each passing day, for example organ transplant, being widely used of antineoplastic, broad-spectrum antibiotic, immunodepressant, various Interventional examination treatments, intravenous hyperalimentation and other catheter retaining etc. all are creating conditions of bacteremia, septicemia.Bacteremia and septicemia disease time are rapid, and mortality ratio is higher.
The incidence of blood infection obviously increases over nearly 20 years, and according to authoritative statistics, present 13% by rising to 6% of the end of the year 70, this does not also comprise bacteremia and septicemia that quite a few is caused by anaerobic infection.Because bacteremia and septicemia are the diseases that seriously jeopardizes clinically patient's life, mortality ratio is up to 29%, and the blood microbe growth is the important evidence of establishing bacteremia and Diagnosis of Septicemia, patient for the severe infections disease, can effectively cure, be decided by to a great extent microbe growth report promptly and accurately.At present, except the good hospital of minority condition dependence on import Automated blood culture instrument device, the most domestic hospital clinical carries out the blood microbe growth, or carries out manual static with manual mode and cultivate, and the microbial growth situation detects by an unaided eye.The shortcomings such as this artificial training method exists that growth of microorganism is poor, incubation time is long, positive rate detects low, poor accuracy, pollution rate is higher far can not adapt to the needs of modern clinic medical treatment.
Existing Full-automatic blood culture instrument mainly is comprised of constant-temperature incubation device, detection system and special culture flask three parts.Blood preparation is inoculated in the nutrient solution of special culture flask, special culture flask does not stop concussion to accelerate microbe growth in the constant-temperature incubation device, also be marked with special receptor indicator in the bottle, bacterium can produce carbon dioxide and cause redox reaction in growth course, make receptor indicator fluorescence excitation and produce change color.Determine the growth of microorganism situation by detecting fluorescence and change color.The core component of Automated blood culture instrument detecting system is the growth of microorganism optical detecting sensor, it is arranged at special culture flask bottom, in bottle in the microorganism growth process, the growth of microorganism optical detecting sensor accurately detects faint change in fluorescence or colourity variation in good time, by opto-electronic conversion and amplifying circuit, by analysis process system record and demonstration bacterial growth situation, draw growth curve, accurately the critical point of the judgement sample positive is also made report.
Summary of the invention
In order to detect and record microbial growth in the special culture flask, the present invention aims to provide a kind of growth of microorganism optical detecting sensor, purpose is to utilize growth of microorganism mechanism and optical principle, provide a kind of high sensitivity, anti-interference, can detect growth of microorganism and be converted into the growth of microorganism detecting sensor that changes electric signal with growth of microorganism quantity direct proportion.
According to growth of microorganism detecting sensor of the present invention, it comprises: light emitting diode matrix, optical filter, photodiode, interior hollow round column body base, light emitting diode constant-current supply, it is characterized in that, described light emitting diode matrix is comprised of a plurality of LED, described optical filter is positioned at hollow round column body center near apical position, a little less than the top; Described photodiode is positioned at the vertical lower of optical filter, the sensitive surface of described photodiode towards optical filter and with the optical filter plane parallel.
Preferably, the number of described a plurality of LED is 4.
Preferably, described a plurality of LED evenly are embedded in around the interior hollow round column body base top, and the position is a little less than the top planes of right cylinder base, and become miter angle to tilt with top planes.
Preferably, described optical filter plane is vertical with the right cylinder axis, and is fixed by thread complete set.
Preferably, the output line of described photodiode connects high sensitivity low noise linear amplifier circuit.
Preferably, described light emitting diode matrix is connected in series and is connected to the light emitting diode constant-current supply, to guarantee the stability of LED brightness.
Preferably, described light emitting diode selects the highlighted LED of same model, same parameter, emission center wavelength 535nm as excitation source.
Preferably, described optical filter is the vacuum coating optical filter.
Preferably, the centre wavelength of described vacuum coating optical filter is 590nm, and bandwidth 10nm is with the interference of effective filtering veiling glare.
Preferably, described photodiode is installed on the photodiode seat, and the photodiode seat is placed on and detects in the base.
More preferably, described detection base is by being screwed on the interior hollow round column body base, and described optical filter is placed on and detects on the base, is fixed on by the detector tube lid and detects on the base.
Compared with prior art, the invention has the beneficial effects as follows:
1, realized the high sensitivity of growth of microorganism is accurately detected, thereby realized the full-automation to blood preparation bacterial growth and detection, solved that the growth of microorganism that the manual mode manual static cultivates is poor, incubation time long, positive rate detects low, the shortcomings such as pollution rate is high, poor accuracy.
2, improved the sensitivity that detects growth of microorganism, sensitivity reaches≤10CFU, and testing result is stable accurately and reliably.
3, antijamming capability is strong, avoids false positive and false negative, and the testing process positive rate is stable, detection time is short, be not easy pollution, and 90% positive sample can be detected in 24 hours, and the Full-automatic blood culture instrument indices that my company is produced reaches international standards.
Description of drawings
Fig. 1 is the three-dimensional perspective that adopts the Full-automatic blood culture instrument of growth of microorganism optical detecting sensor of the present invention;
Fig. 2 is the structural representation according to growth of microorganism optical detecting sensor of the present invention, and wherein, 401 is that LED, 402 is that optical filter, 404 is that interior hollow round column body base, 405 is the light emitting diode constant-current supply;
Fig. 3 is the sectional view according to growth of microorganism optical detecting sensor of the present invention.
Embodiment
The present invention is further described below in conjunction with drawings and Examples, but not as a limitation of the invention.
Consult now Fig. 1, Fig. 1 is the three-dimensional perspective that adopts the Full-automatic blood culture instrument of growth of microorganism optical detecting sensor of the present invention.In Fig. 1, Full-automatic blood culture instrument comprises: the rotating disk that is comprised of rotating disk header board 101, sleeve 103 and end cap 102; The machine driven system that turning axle 204, shaft coupling 201, reductor 202 and stepper motor 203 form; The position control system that locating stop piece 301, start bit position transducer 302 form; Growth of microorganism optical detecting sensor 4 and firm banking 5.Rotating disk links to each other with stepper motor 203 by turning axle 204, locating stop piece 301 is fixed on disk edge, start bit position transducer 302 is fixed on the rotating disk below, and locating stop piece 301, start bit position transducer 302 all link to each other with the signaling control unit (not shown) of Full-automatic blood culture instrument with stepper motor 203.
Continuation referring to figs. 2 and 3, Fig. 2 is the structural representation according to growth of microorganism optical detecting sensor of the present invention, Fig. 3 is the sectional view according to growth of microorganism optical detecting sensor of the present invention.Growth of microorganism optical detecting sensor of the present invention comprises: light emitting diode matrix 401, optical filter 402, photodiode 403, interior hollow round column body base 404, light emitting diode constant-current supply 405.Light emitting diode matrix 401 is composed in series by 3-8 LED, is preferably 4, evenly is embedded in around the top of interior hollow round column body base 404, and the position is a little less than interior hollow round column body base 404 top planes, and becomes miter angle to tilt with top planes.Photodiode 403 is installed on the photodiode seat 406, photodiode seat 406 is placed on and detects in the base 407, detect base 407 by being screwed on the interior hollow round column body base 404, optical filter 402 is placed on and detects on the base 407, is fixed on by detector tube lid 408 and detects on the base 407.Photodiode 403 is fixed on the bottom of optical filter 402, sensitive surface towards optical filter 402 and with optical filter 402 plane parallel, photodiode output line 409 connects high sensitivity low noise linear amplifier circuit.
Described 4 light emitting diodes are connected in series and are connected to the constant current source power supply circuit of LED, to guarantee the stability of LED brightness.It is the highlighted LED of 535nm that 4 light emitting diodes are selected same model, same parameter, emission center wavelength, as excitation source.
Described optical filter 402 is the vacuum coating optical filter, and centre wavelength is 590nm, bandwidth 10nm, and narrow bandwidth is the interference of filtering veiling glare effectively.
Light emitting diode illuminates at the bottom of the special culture flask with miter angle, for the voltage sensor indicator in the special culture flask polymeric membrane provides excitation source, adopt 4 just in order to have enough brightness and brightness even, simultaneously also in order to guarantee that photodiode is in the linear work district.The light emitting diode constant-current supply guarantees stable luminescence.
Growth of microorganism detecting sensor of the present invention cooperates a kind of special culture flask usually, at the bottom of this culture flask bottle one deck polymeric membrane is arranged, evenly mixed a kind of voltage sensor indicator in the film, bacterium can produce redox reaction in growth course, cause the variation of nutrient culture media inner potential, the receptor indicator is stimulated produces fluorescence and colourity variation, the variation of this variation and micro organism quantity and the change list of oxidation-reduction potential reveal good consistance.This redox potentiometry detects than the carbon dioxide chromatmetry, has higher stability and sensitivity.The excitation wavelength of this preferred receptor indicator is 550nm, and the fluorescent emission wavelength is 590nm.
Above-described embodiment, just the preferred embodiments of the disclosure is a kind of, and the common variation that those skilled in the art carries out in the technical solution of the present invention scope and replacement all should be included in protection scope of the present invention.
Claims (11)
1. growth of microorganism optical detecting sensor, it comprises: light emitting diode matrix (401), optical filter (402), photodiode (403), interior hollow round column body base (404), light emitting diode constant-current supply (405), it is characterized in that, described light emitting diode matrix (401) is comprised of a plurality of LED, described optical filter (402) is positioned at hollow round column body center near apical position, a little less than the top; Described photodiode (403) is positioned at the vertical lower of optical filter (402), the sensitive surface of described photodiode (403) towards optical filter (402) and with the optical filter plane parallel.
2. growth of microorganism optical detecting sensor according to claim 1 is characterized in that, the number of described a plurality of LED is 4.
3. growth of microorganism optical detecting sensor according to claim 1 and 2, it is characterized in that, described a plurality of LED evenly is embedded in around interior hollow round column body base (404) top, the position is a little less than the top planes of interior hollow round column body base (404), and becomes miter angle to tilt with top planes.
4. growth of microorganism optical detecting sensor according to claim 3 is characterized in that, described optical filter plane is vertical with right cylinder base (404) axis, and is fixed by thread complete set.
5. growth of microorganism optical detecting sensor according to claim 3 is characterized in that, the output line of described photodiode (403) connects high sensitivity low noise linear amplifier circuit.
6. growth of microorganism optical detecting sensor according to claim 1 and 2 is characterized in that, described light emitting diode array (401) is connected in series and is connected to light emitting diode constant-current supply (405), to guarantee the stability of LED brightness.
7. growth of microorganism optical detecting sensor according to claim 6 is characterized in that, described light emitting diode selects the highlighted LED of same model, same parameter, emission center wavelength 535nm as excitation source.
8. growth of microorganism optical detecting sensor according to claim 1 is characterized in that, described optical filter (402) is the vacuum coating optical filter.
9. growth of microorganism optical detecting sensor according to claim 8 is characterized in that, the centre wavelength of described vacuum coating optical filter is 590nm, and bandwidth 10nm is with the interference of effective filtering veiling glare.
10. growth of microorganism optical detecting sensor according to claim 1 and 2, it is characterized in that, described photodiode (403) is installed on the photodiode seat (406), and photodiode seat (406) is placed on and detects in the base (407).
11. growth of microorganism optical detecting sensor according to claim 10, it is characterized in that, described detection base (407) is by being screwed on the interior hollow round column body base (404), described optical filter (402) is placed on and detects on the base (407), is fixed on by detector tube lid (408) and detects on the base (407).
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| CN2012105834231A CN103063639A (en) | 2012-12-31 | 2012-12-31 | Microbial growth optical detection sensor |
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| CN2012105834231A CN103063639A (en) | 2012-12-31 | 2012-12-31 | Microbial growth optical detection sensor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555562A (en) * | 2013-11-01 | 2014-02-05 | 山东鑫科生物科技股份有限公司 | Blood culture instrument |
| CN108139326A (en) * | 2015-09-30 | 2018-06-08 | 剑桥显示技术有限公司 | With low background signal based on organic fluorescent optical sensor |
| CN109880879A (en) * | 2019-02-28 | 2019-06-14 | 张家港高品诚医械科技有限公司 | A kind of Bacteria Culture detection method |
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| JP2002350349A (en) * | 2001-05-22 | 2002-12-04 | Matsushita Electric Ind Co Ltd | Fluorescence detector |
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| JP2012255707A (en) * | 2011-06-08 | 2012-12-27 | Olympus Corp | Fluorescence sensor |
| CN203025088U (en) * | 2012-12-31 | 2013-06-26 | 山东鑫科生物科技股份有限公司 | Optical detection sensor for microbial growth |
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2012
- 2012-12-31 CN CN2012105834231A patent/CN103063639A/en active Pending
Patent Citations (6)
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| JP2002350349A (en) * | 2001-05-22 | 2002-12-04 | Matsushita Electric Ind Co Ltd | Fluorescence detector |
| JP2006098227A (en) * | 2004-09-29 | 2006-04-13 | Fuji Photo Film Co Ltd | Reflected light measuring instrument and biochemical analyzer |
| CN201152848Y (en) * | 2007-10-31 | 2008-11-19 | 杨力 | Bionic intelligent type blood rheogeniometer |
| CN201890881U (en) * | 2010-11-18 | 2011-07-06 | 山东鑫科生物科技股份有限公司 | Automated blood culture instrument detecting system |
| JP2012255707A (en) * | 2011-06-08 | 2012-12-27 | Olympus Corp | Fluorescence sensor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555562A (en) * | 2013-11-01 | 2014-02-05 | 山东鑫科生物科技股份有限公司 | Blood culture instrument |
| CN108139326A (en) * | 2015-09-30 | 2018-06-08 | 剑桥显示技术有限公司 | With low background signal based on organic fluorescent optical sensor |
| CN109880879A (en) * | 2019-02-28 | 2019-06-14 | 张家港高品诚医械科技有限公司 | A kind of Bacteria Culture detection method |
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Application publication date: 20130424 |