CN203025088U - Optical detection sensor for microbial growth - Google Patents
Optical detection sensor for microbial growth Download PDFInfo
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- CN203025088U CN203025088U CN 201220752715 CN201220752715U CN203025088U CN 203025088 U CN203025088 U CN 203025088U CN 201220752715 CN201220752715 CN 201220752715 CN 201220752715 U CN201220752715 U CN 201220752715U CN 203025088 U CN203025088 U CN 203025088U
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Abstract
The utility model relates to a medical detection apparatus, and in particular discloses an optical detection sensor for microbial growth. The optical detection sensor comprises a light emitting diode (LED) array (401) composed of four LEDs, a light filter (402), a photoelectric diode (403), a hollow cylindrical base (404) and an LED constant-current power supply (405), wherein the light filter (402) is a vacuum coated light filter. The optical detection sensor can perform high-sensitivity precision detection on microbial growth by utilizing a fluorescence principle and a colorimetric principle in a microbial growth process, and therefore, full-automation growth and detection of blood preparation bacteria are realized.
Description
Technical field
The utility model relates to medical instruments and equipment, in particular to a kind of growth of microorganism optical detecting sensor for Automated blood culture instrument detecting system.
Background technology
Progress due to medical skill, various diagnoses and treatment means grow with each passing day, for example organ transplant, being widely used of antineoplastic, broad-spectrum antibiotic, immunodepressant, various Interventional examination treatments, intravenous hyperalimentation and other catheter retaining etc. are all creating conditions of bacteremia, septicemia.Bacteremia and septicemia disease time are rapid, and mortality ratio is higher.
Over nearly 20 years, the incidence of blood infection obviously increases, and according to authoritative statistics, present 13% by rising to 6% of the end of the year 70, this does not also comprise bacteremia and septicemia that quite a few is caused by anaerobic infection.Because bacteremia and septicemia are seriously to jeopardize clinically the disease of patient's life, mortality ratio is up to 29%, and the blood microbe growth is the important evidence of establishing bacteremia and Diagnosis of Septicemia, patient for the severe infections disease, can effectively cure, be decided by to a great extent microbe growth report promptly and accurately.At present, except minority condition good hospital dependence on import Automated blood culture instrument device, the most domestic hospital clinical carries out the blood microbe growth, or carries out manual static with manual mode and cultivate, and the microbial growth situation detects by an unaided eye.The shortcomings such as this artificial training method exists that growth of microorganism is poor, incubation time is long, positive rate detects low, poor accuracy, pollution rate is higher far can not adapt to the needs of modern clinic medical treatment.
Existing Full-automatic blood culture instrument mainly is comprised of constant-temperature incubation device, detection system and special culture flask three parts.Blood preparation is inoculated in the nutrient solution of special culture flask, special culture flask does not stop concussion to accelerate microbe growth in the constant-temperature incubation device, also be marked with special receptor indicator in bottle, bacterium can produce carbon dioxide and cause redox reaction in growth course, makes receptor indicator fluorescence excitation and produces change color.Determine the growth of microorganism situation by detecting fluorescence and change color.The core component of Automated blood culture instrument detecting system is the growth of microorganism optical detecting sensor, it is arranged at bottom special culture flask, in bottle in microorganism growth process, the growth of microorganism optical detecting sensor accurately detects faint change in fluorescence or colourity variation in good time, by opto-electronic conversion and amplifying circuit, recorded and shown the bacterial growth situation by analysis process system, drawing growth curve, the critical point of the accurate judgement sample positive is also made report.
The utility model content
In order to detect and record microbial growth in special culture flask, the utility model aims to provide a kind of growth of microorganism optical detecting sensor, purpose is to utilize growth of microorganism mechanism and optical principle, provide a kind of high sensitivity, anti-interference, growth of microorganism can be detected and be converted into the growth of microorganism detecting sensor that changes electric signal with growth of microorganism quantity direct proportion.
According to growth of microorganism detecting sensor of the present utility model, it comprises: light emitting diode array, optical filter, photodiode, interior hollow round column body base, light emitting diode constant-current supply, it is characterized in that, described light emitting diode matrix is comprised of a plurality of LED, described optical filter is positioned at the close apical position in hollow round column body center, a little less than the top; Described photodiode is positioned at the vertical lower of optical filter, the sensitive surface of described photodiode towards optical filter and with the optical filter plane parallel.
Preferably, the number of described a plurality of LED is 4.
Preferably, described a plurality of LED evenly are embedded in interior hollow round column body base top surrounding, and the position is a little less than the top plan of right cylinder base, and become miter angle to tilt with top plan.
Preferably, described optical filter plane is vertical with the right cylinder axis, and is fixed by thread complete set.
Preferably, the output line of described photodiode connects high sensitivity low noise linear amplifier circuit.
Preferably, described light emitting diode array is connected in series and is connected to the light emitting diode constant-current supply, to guarantee the stability of LED brightness.
Preferably, described light emitting diode selects the highlighted LED of same model, same parameter, emission center wavelength 535nm as excitation source.
Preferably, described optical filter is the vacuum coating optical filter.
Preferably, the centre wavelength of described vacuum coating optical filter is 590nm, and bandwidth 10nm is with the interference of effective filtering veiling glare.
Preferably, described photodiode is arranged on the photodiode seat, and the photodiode seat is placed on and detects in base.
More preferably, described detection base is by being screwed on interior hollow round column body base, and described optical filter is placed on and detects on base, is fixed on by the detector tube lid and detects on base.
Compared with prior art, the beneficial effects of the utility model are:
1, realized the high sensitivity of growth of microorganism is accurately detected, thereby realized the full-automation to blood preparation bacterial growth and detection, solved that the growth of microorganism that the manual mode manual static cultivates is poor, incubation time long, positive rate detects low, the shortcomings such as pollution rate is high, poor accuracy.
2, improved the sensitivity that detects growth of microorganism, sensitivity reaches≤10CFU, and testing result is stable accurately and reliably.
3, antijamming capability is strong, avoids false positive and false negative, and the testing process positive rate is stable, detection time is short, be not easy pollution, and 90% positive sample can be detected in 24 hours, and the Full-automatic blood culture instrument indices that my company is produced reaches international standards.
Description of drawings
Fig. 1 is the three-dimensional perspective that adopts the Full-automatic blood culture instrument of growth of microorganism optical detecting sensor of the present utility model;
Fig. 2 is the structural representation according to growth of microorganism optical detecting sensor of the present utility model, and wherein, 401 is that light emitting diode LED, 402 is that optical filter, 404 is that interior hollow round column body base, 405 is the light emitting diode constant-current supply;
Fig. 3 is the sectional view according to growth of microorganism optical detecting sensor of the present utility model.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further illustrated, but not as to restriction of the present utility model.
Consult now Fig. 1, Fig. 1 is the three-dimensional perspective that adopts the Full-automatic blood culture instrument of growth of microorganism optical detecting sensor of the present utility model.In Fig. 1, Full-automatic blood culture instrument comprises: the rotating disk that is comprised of rotating disk header board 101, sleeve 103 and end cap 102; The machine driven system that turning axle 204, shaft coupling 201, reductor 202 and stepper motor 203 form; The position control system that locating stop piece 301, start bit position transducer 302 form; Growth of microorganism optical detecting sensor 4 and firm banking 5.Rotating disk is connected with stepper motor 203 by turning axle 204, locating stop piece 301 is fixed on disk edge, start bit position transducer 302 is fixed on below rotating disk, and locating stop piece 301, start bit sensor 302 all are connected with the signaling control unit (not shown) of Full-automatic blood culture instrument with stepper motor 203.
Continuation referring to figs. 2 and 3, Fig. 2 is the structural representation according to growth of microorganism optical detecting sensor of the present utility model, Fig. 3 is the sectional view according to growth of microorganism optical detecting sensor of the present utility model.Growth of microorganism optical detecting sensor of the present utility model comprises: light emitting diode array 401, optical filter 402, photodiode 403, interior hollow round column body base 404, light emitting diode constant-current supply 405.Light emitting diode matrix 401 is composed in series by 3-8 LED, is preferably 4, evenly is embedded in the top surrounding of interior hollow round column body base 404, and the position is a little less than interior hollow round column body base 404 top plan, and becomes miter angle to tilt with top plan.Photodiode 403 is arranged on photodiode seat 406, photodiode seat 406 is placed on and detects in base 407, detect base 407 by being screwed on interior hollow round column body base 404, optical filter 402 is placed on and detects on base 407, is fixed on by detector tube lid 408 and detects on base 407.Photodiode 403 is fixed on the bottom of optical filter 402, sensitive surface towards optical filter 402 and with optical filter 402 plane parallel, photodiode output line 409 connects high sensitivity low noise linear amplifier circuit.
Described 4 light emitting diodes are connected in series and are connected to the constant current source power supply circuit of LED, to guarantee the stability of LED brightness.It is the highlighted LED of 535nm that 4 light emitting diodes are selected same model, same parameter, emission center wavelength, as excitation source.
Described optical filter 402 is the vacuum coating optical filter, and centre wavelength is 590nm, bandwidth 10nm, and narrow bandwidth is the interference of filtering veiling glare effectively.
At the bottom of light emitting diode illuminates special culture flask with miter angle, for the voltage sensor indicator in special culture flask polymeric membrane provides excitation source, adopt 4 just in order to have enough brightness and brightness even, simultaneously also in order to guarantee that photodiode is in the linear work district.The light emitting diode constant-current supply guarantees stable luminescence.
Growth of microorganism detecting sensor of the present utility model coordinates a kind of special culture flask usually, at the bottom of this culture flask bottle, one deck polymeric membrane is arranged, evenly mixed a kind of voltage sensor indicator in film, bacterium can produce redox reaction in growth course, cause the variation of nutrient culture media inner potential, the receptor indicator is stimulated produces fluorescence and colourity variation, the variation of this variation and micro organism quantity and the change list of oxidation-reduction potential reveal good consistance.This redox potentiometry detects than the carbon dioxide chromatmetry, has higher stability and sensitivity.The excitation wavelength of this preferred receptor indicator is 550nm, and the fluorescent emission wavelength is 590nm.
Above-described embodiment, just the preferred embodiment of the utility model is a kind of, and the common variation that those skilled in the art carries out in the technical solutions of the utility model scope and replacement all should be included in protection domain of the present utility model.
Claims (11)
1. growth of microorganism optical detecting sensor, it comprises: light emitting diode matrix (401), optical filter (402), photodiode (403), interior hollow round column body base (404), light emitting diode constant-current supply (405), it is characterized in that, described light emitting diode matrix (401) is comprised of a plurality of LED, described optical filter (402) is positioned at the close apical position in hollow round column body center, a little less than the top; Described photodiode (403) is positioned at the vertical lower of optical filter (402), the sensitive surface of described photodiode (403) towards optical filter (402) and with the optical filter plane parallel.
2. growth of microorganism optical detecting sensor according to claim 1, is characterized in that, the number of described a plurality of LED is 4.
3. growth of microorganism optical detecting sensor according to claim 1 and 2, it is characterized in that, described a plurality of LED evenly is embedded in interior hollow round column body base top surrounding, and the position is a little less than the top plan of right cylinder base (404), and becomes miter angle to tilt with top plan.
4. growth of microorganism optical detecting sensor according to claim 3, is characterized in that, described optical filter plane is vertical with interior hollow round column body base (404) axis, and is fixed by thread complete set.
5. growth of microorganism optical detecting sensor according to claim 3, is characterized in that, the output line of described photodiode (403) connects high sensitivity low noise linear amplifier circuit.
6. growth of microorganism optical detecting sensor according to claim 1 and 2, is characterized in that, described light emitting diode matrix (401) is connected in series and is connected to light emitting diode constant-current supply (405), to guarantee the stability of LED brightness.
7. growth of microorganism optical detecting sensor according to claim 6, is characterized in that, described light emitting diode selects the highlighted LED of same model, same parameter, emission center wavelength 535nm as excitation source.
8. growth of microorganism optical detecting sensor according to claim 1, is characterized in that, described optical filter (402) is the vacuum coating optical filter.
9. growth of microorganism optical detecting sensor according to claim 8, is characterized in that, the centre wavelength of described vacuum coating optical filter is 590nm, and bandwidth 10nm is with the interference of effective filtering veiling glare.
10. growth of microorganism optical detecting sensor according to claim 1 and 2, it is characterized in that, described photodiode (403) is arranged on photodiode seat (406), and photodiode seat (406) is placed on and detects in base (407).
11. growth of microorganism optical detecting sensor according to claim 10, it is characterized in that, described detection base (407) is by being screwed on interior hollow round column body base (404), described optical filter (402) is placed on and detects on base (407), is fixed on by detector tube lid (408) and detects on base (407).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220752715 CN203025088U (en) | 2012-12-31 | 2012-12-31 | Optical detection sensor for microbial growth |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220752715 CN203025088U (en) | 2012-12-31 | 2012-12-31 | Optical detection sensor for microbial growth |
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| CN203025088U true CN203025088U (en) | 2013-06-26 |
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| CN 201220752715 Expired - Lifetime CN203025088U (en) | 2012-12-31 | 2012-12-31 | Optical detection sensor for microbial growth |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063639A (en) * | 2012-12-31 | 2013-04-24 | 山东鑫科生物科技股份有限公司 | Microbial growth optical detection sensor |
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2012
- 2012-12-31 CN CN 201220752715 patent/CN203025088U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063639A (en) * | 2012-12-31 | 2013-04-24 | 山东鑫科生物科技股份有限公司 | Microbial growth optical detection sensor |
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Addressee: Du Junwei Document name: Notification of Acceptance of Patent Application |
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| GR01 | Patent grant | ||
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| CX01 | Expiry of patent term |
Granted publication date: 20130626 |