CN205449803U - Appearance detecting system is cultivateed to full -automatic body fluid - Google Patents
Appearance detecting system is cultivateed to full -automatic body fluid Download PDFInfo
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- CN205449803U CN205449803U CN201620127467.7U CN201620127467U CN205449803U CN 205449803 U CN205449803 U CN 205449803U CN 201620127467 U CN201620127467 U CN 201620127467U CN 205449803 U CN205449803 U CN 205449803U
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- sensor
- circuit
- body fluid
- detecting system
- light source
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- 210000001124 body fluid Anatomy 0.000 title claims abstract description 21
- 239000010839 body fluid Substances 0.000 title claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 230000005284 excitation Effects 0.000 claims abstract description 8
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 6
- 230000035945 sensitivity Effects 0.000 abstract description 10
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 20
- 244000005700 microbiome Species 0.000 description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 description 10
- 239000001569 carbon dioxide Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 208000031729 Bacteremia Diseases 0.000 description 1
- 206010048612 Hydrothorax Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002696 acid base indicator Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000001795 light effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to an appearance detecting system is cultivateed to full -automatic body fluid. The detection module who is located bottom the culture tank who cultivates appearance box inner room by a plurality of branches constitutes, detection module including respectively from two orientations incide the blake bottle the sensor bottom, respectively as two LED light sources of incident light source and excitation light source, all be equipped with collimating lens at the exit end of each LED light source, be equipped with in the below of sensor and carry out the collector lens that assembles to the light via the sensor scattering, be equipped with photodiode in collector lens's focus department, still be equipped with the light filter between sensor and collector lens, still include detection circuitry, detection circuitry is including the preamplification circuit, main amplifier circuit, demodulation circuit and the AD converting circuit that connect in order, photodiode and preamplification circuit electric connection. The utility model discloses an its positive rate of accuracy of detecting system, sensitivity and stability are high to provide the testing result of clinical body fluid sample in the time short as far as possible, simultaneously reduce cost.
Description
Technical field
This utility model belongs to medical facility technology field, particularly relates to a kind of full-automatic body fluid and cultivates instrument detecting system.
Background technology
It is for detecting a kind of microbiological test method existed in human sample with or without microorganism that body fluid is cultivated, including hemoculture, Sputum culturing, urine cultivation and other body fluid (hydrothorax, ascites, cerebrospinal fluid etc.) and the cultivation of analog.The septicemia of patient vitals, bacteremia are the most seriously jeopardized for quickly detection, whether patient body fluid has bacterial growth with highly important effect of having clarified a diagnosis, be the key of clinical effectively treatment.So the detection of pathogenic microorganism should be carried out within the shortest time in body fluid or the like.In recent years, along with scientific and technological progress, the combination of microbiology, computer and engineering, the culture systems of multiple automatization has been createed.
The culture systems of these automatizatioies, is made up of the culture bottle of the instrument cultivated and detect with matching used belt sensor, and one is to utilize optical detection principle, the growing state of microorganism in continuous print monitoring culture bottle.This detecting system is at one carbon dioxide sensor with aqueous indicator of each culture bottle bottom device, when the culture medium in culture bottle has growth of microorganism, produce carbon dioxide, along with the raising of gas concentration lwevel can cause the color of indicator to change, above sensor light emitting diode send light and project on sensor with 45° angle, measured its scattered light by a photodetector again, determine whether growth of microorganism according to the intensity of scattered light.This system can carry out the growing state automatically detecting culture bottle microorganism of continuous print Noninvasive, but is the most necessarily restricted, and causes the sensitivity of detecting system and stability relatively low, and flashing will also result in the wrong report of result surely.Another kind is containing fluorescent dye in sensor, when the growth of microorganism in culture bottle produces carbon dioxide, carbon dioxide reacts with fluorescent dye, excitation source above sensor excites fluorescence, bottom photodetector measures the intensity of fluorescence, determines whether growth of microorganism according to fluorescence intensity.Although this detecting system is more stable, but its can not by artificial interpretation, can only in instrument Aulomatizeted Detect, but its detection equipment manufacturing cost higher.
Utility model content
This utility model provides a kind of full-automatic body fluid to cultivate instrument detecting system for solving technical problem present in known technology, solve positive accuracy rate, sensitivity and the stability problem of detection equipment, and within the shortest time, provide the positive findings whether containing pathogenic microorganism in clinic body fluid sample, reduce cost simultaneously.
This utility model is adopted the technical scheme that by solving technical problem present in known technology: a kind of full-automatic body fluid is cultivated the detection modules that instrument detecting system is positioned at by multiple points bottom the culture tank of cultivation instrument casing room and constituted；Detection module include respectively from both direction incide culture bottle sensor base, respectively as two LED light sources of incident light source and excitation source, exit end at each LED light source is equipped with collimating lens, be provided with the collecting lens converging the light scattered via sensor and the fluorescent light excited in the lower section of sensor, the focal point at collecting lens is provided with photodiode；Light path between the bottom and collecting lens of sensor is additionally provided with optical filter；Also including testing circuit, testing circuit includes that pre-amplification circuit, main amplifying circuit, demodulator circuit and the A/D change-over circuit being sequentially connected with, photodiode are electrically connected with pre-amplification circuit.
Advantage of the present utility model and good effect be: this utility model provides the full-automatic body fluid of a kind of reasonable in design and cultivates the detecting system of instrument, compared with existing detecting system, the technical program is by using the double light source of LED as incident light source and excitation source, ensure that the strength and stability of light source, improve the sensitivity of detection.And utilize photodiode to convert light signals into the signal of telecommunication to process；What double light signal collections gathered the scattered light of sensor and fluorescent material respectively excites fluorescence, existing two kinds of technology are combined, decrease signal disturbing, improve sensitivity and accuracy that light is differentiated, and then improve accuracy rate and the sensitivity of detection equipment.Detection device sensitivity and the raising of accuracy rate, can shorten the Clinical Laboratory cycle, reduces the patient treatment time, and the accuracy rate of raising can make patient be given treatment in time, it is to avoid the state of an illness is delayed.
Preferably: two LED light sources all project on sensor with 45° angle.
Preferably: described testing circuit is double testing circuit structures.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structured flowchart of testing circuit in this utility model.
In figure: 1, LED light source；2, collimating lens；3, collecting lens；4, photodiode；5, testing circuit；6, sensor；7, optical filter；8, pre-amplification circuit；9, main amplifying circuit；10, demodulator circuit；11, A/D change-over circuit；12, control system.
Detailed description of the invention
For summary of the invention of the present utility model, feature and effect can be further appreciated that, hereby lift following example and describe in detail as follows:
Referring to Fig. 1 and Fig. 2, full-automatic body fluid of the present utility model is cultivated the detecting system of instrument and is made up of the multiple points of detection modules being positioned at bottom the culture tank cultivating instrument casing room.
Detection module includes inciding the LED light source 1 bottom the sensor 6 of culture bottle from both direction respectively, one of them LED light source 1 as incident light source, another LED light source 1 as excitation source, there is scattering and form scattered light in the incident ray that incident light source produces at sensor 6, the excitation line that excitation source produces makes sensor 6 produce fluorescence.Exit end at each LED light source 1 is equipped with collimating lens 2, the collecting lens 3 that the light scattered via sensor 6 is converged it is provided with in the lower section of sensor 6, focal point at collecting lens 3 is provided with photodiode 4, collecting lens 3, by scattered light and fluorescent foci to photodiode 4, is formed the signal of telecommunication by photodiode 4.In the present embodiment, two LED light sources 1 all project on sensor 6 with 45° angle.
Optical filter 7 it is additionally provided with, for scattered light and fluorescence are filtered in light path between the bottom of sensor 6 and collecting lens 3.By optical filter is adjusted, scattered light light and fluorescent light alternately through and detected.Two LED light sources 1 select a wave band that can simultaneously work as incident illumination and exciting light effect, incide the bottom of sensor 6, scattered light and the fluorescence excited improve the sensitivity of equipment by double light source detection systems, fluorescence signal, and scattered light signal improves the stability of equipment.
Also include testing circuit 5, pre-amplification circuit 8 that testing circuit 5 includes being sequentially connected with, main amplifying circuit 9, demodulator circuit 10 and A/D change-over circuit 11, photodiode 4 is electrically connected with pre-amplification circuit 8, and A/D change-over circuit 11 is connected to cultivate the control system 12 of instrument.In the present embodiment, testing circuit 5 is double testing circuit structures, and double testing circuits 5 can improve the stability of detecting system.
Its testing circuit of detecting system that full-automatic body fluid cultivates instrument includes pre-amplification circuit 8, main amplifying circuit 9, demodulator circuit 10 and A/D change-over circuit 11.The amplitude of signal and noise is improved to certain level by pre-amplification circuit 8, main amplifying circuit 9 carries out linear amplification, signal after demodulator circuit 10 will amplify carries out square wave demodulation, carries out A/D conversion, then signal metering-in control system 12 in the way of digital quantity at A/D change-over circuit 11 afterwards.After two kinds of light signal metering-in control systems 12, through double light source processing systems, it is fitted to together, forms growth curve intuitively.
The method that full-automatic body fluid cultivates instrument detection: in the body fluid culture bottle with sensor 6 and culture medium, the sample having microorganism is suspected in inoculation, is loaded into full-automatic body fluid and cultivates in instrument；When there is growth of microorganism in sample and producing carbon dioxide, carbon dioxide, through the semipermeable membrane of sensor 6, changes the acid or alkali environment of sensor 6, makes the acid-base indicator color in sensor 6 change, such as, become yellow from blueness or become light color from dark color；Color change number be converted into digital quantity through detecting system and show in control system, to determine the situation of growth of microorganism.Carbon dioxide is as the by-product of growth of microorganism, the generation amount of every kind of microorganism is the most different, the carbon dioxide produced during some growth of microorganism is less, or the carbon dioxide that microbial activity is poor and produces is less, the color change making sensor 6 is less, and then detecting system is when judging signal, may think that and is not enough to constitute the condition that report is positive, and now microorganism grown really, thus result in false negative.
In order to detect color change delicately, use double LED light source 1 incident from both direction, light intensity can be increased, and then increase the intensity of scattered light.The light of scattering is received by probe unit, is easier to monitor the color change of sensor 6, improves the sensitivity of detection when the light intensity of reception is bigger.Probe unit can not only accept the scattered light of sensor, and has the function receiving fluorescence, carbon dioxide and the reacted fluorogenic dye in sensor 6, generates fluorescent material, and the incident illumination of LED light source 1 can excite fluorescent material, send fluorescence.Receive scattered light and fluorescence, it is possible to reduce noise jamming simultaneously, improve sensitivity and the accuracy rate of detecting system, reduce false negative.
Embodiment described above is merely to illustrate technological thought of the present utility model and feature, its object is to make those skilled in the art it will be appreciated that content of the present utility model implementing according to this, only can not limit the scope of the claims of the present utility model with the present embodiment, what the spirit disclosed in the most all this utility model was made changes on an equal basis or modifies, and still falls in the scope of the claims of the present utility model.
Claims (3)
1. full-automatic body fluid cultivates an instrument detecting system, it is characterized in that: be made up of the multiple points of detection modules being positioned at bottom the culture tank cultivating instrument casing room；Detection module includes inciding two LED light sources (1) bottom the sensor (6) of culture bottle, respectively as incident light source and excitation source respectively from both direction, exit end at each LED light source (1) is equipped with collimating lens (2), be provided with the collecting lens (3) converging the light scattered via sensor (6) and the fluorescent light excited in the lower section of sensor (6), the focal point at collecting lens (3) is provided with photodiode (4)；Optical filter (7) it is additionally provided with in light path between the bottom of sensor (6) and collecting lens (3)；Also include testing circuit (5), testing circuit (5) includes that pre-amplification circuit (8), main amplifying circuit (9), demodulator circuit (10) and the A/D change-over circuit (11) being sequentially connected with, photodiode (4) are electrically connected with pre-amplification circuit (8).
2. full-automatic body fluid as claimed in claim 1 cultivates instrument detecting system, it is characterized in that: two LED light sources (1) all project on sensor (6) with 45° angle.
3. full-automatic body fluid as claimed in claim 1 cultivates instrument detecting system, it is characterized in that: described testing circuit (5) is double testing circuit structures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620127467.7U CN205449803U (en) | 2016-02-18 | 2016-02-18 | Appearance detecting system is cultivateed to full -automatic body fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620127467.7U CN205449803U (en) | 2016-02-18 | 2016-02-18 | Appearance detecting system is cultivateed to full -automatic body fluid |
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| Publication Number | Publication Date |
|---|---|
| CN205449803U true CN205449803U (en) | 2016-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620127467.7U Expired - Fee Related CN205449803U (en) | 2016-02-18 | 2016-02-18 | Appearance detecting system is cultivateed to full -automatic body fluid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109880879A (en) * | 2019-02-28 | 2019-06-14 | 张家港高品诚医械科技有限公司 | A kind of Bacteria Culture detection method |
-
2016
- 2016-02-18 CN CN201620127467.7U patent/CN205449803U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109880879A (en) * | 2019-02-28 | 2019-06-14 | 张家港高品诚医械科技有限公司 | A kind of Bacteria Culture detection method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 |
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| CF01 | Termination of patent right due to non-payment of annual fee |