CN105548128A - Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method - Google Patents
Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method Download PDFInfo
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- CN105548128A CN105548128A CN201610093590.6A CN201610093590A CN105548128A CN 105548128 A CN105548128 A CN 105548128A CN 201610093590 A CN201610093590 A CN 201610093590A CN 105548128 A CN105548128 A CN 105548128A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
- G01N2201/12746—Calibration values determination
- G01N2201/12761—Precalibration, e.g. for a given series of reagents
Abstract
The invention discloses a method and device for detecting chlorophyll of a coastal zone water body in situ through a double optical path method. According to the method, the concentration of chlorophyll alpha is detected on line on the basis of a fluorescence method detection principle by directly measuring the fluorescence intensity; the device is composed of a light source module, a measuring module, a reference module and a signal processing module. Under excitation of an LED light source, the intensity of fluorescence emitted by the chlorophyll is measured, and the chlorophyll concentration of the coastal zone water body to be detected is calculated through a linearly dependent coefficient calculated by a system model and a compensation value for correcting yellow matter CDOM. The device can quickly detect the concentration of the chlorophyll in the water body on site in real time in a large area and is small in weight and size, convenient to carry, high in accuracy and sensitivity, easy to operate, low in cost and capable of being applied to multiple detection fields.
Description
Technical field
The invention belongs to water environment optical method in situ detection technical field, specifically a kind of bifocal path technique coastal waters chlorophyllogen position monitoring method and realize the device of the method.
Background technology
Chlorophyll is almost present in all phytoplanktons, is one of Content and distribution situation leading indicator estimating phytoplankton and algae.The concentration of chlorophyll a can be used for monitoring station and research institution understands, analyzes Water Eutrophication degree and halobiontic distribution etc., and closely related with culture fishery and fishery etc., is the conventional sense project detecting water quality.
The main method that water body chlorophyll content is measured by current China has spectrophotometric method, high performance liquid chromatography and fluorescence method etc.Spectrophotometric method and high performance liquid chromatography all need lab samples, extract water sample Determination of Chlorophyll is extracted after carry out analysis by spectrophotometric method again and measure, although test data accurately and reliably, but measuring process is loaded down with trivial details, the time is long, data are few, restricted when being applied to real time monitoring.Along with the continuous progress of fluorescence measurement amount water body chlorophyll research, research and development have high accurancy and precision, highly sensitive real-time online chlorophyll analyser has been a focus in present Water-environment Ecological System monitoring field.The fluorescence method that the present invention adopts is more suitable for the extremely low situation of chlorophyll content than spectrophotometric method, detection sensitivity is higher, and can be used for the various water bodies Site Detections such as river, lake and ocean, can Real-Time Monitoring phytoplankton, algae equal distribution, growth and variable condition.
Tradition chlorophyll analyser major part is cabinet type structure, and volume is large, and cost is high, extracts water sample and carries out collecting test.
Summary of the invention
The present invention adopts Fluorometric assay principle, and primarily of light source module, measurement module, referrer module and signal processing module four part composition, the scene that achieves is real-time, quick, the object of large area, precisely monitoring water body chlorophyll concentration.Because tested water body Determination of Chlorophyll α is under the exciting of additional blue-light source, fluorescence can be discharged near longer wavelength 685nm, can on-line monitoring chlorophyll α concentration by directly measuring fluorescence intensity.The dichroic light splitting piece of the blue light that light source module sends in measurement module, major part light reflected after by convex lens in quartz window prefocusing, the collimated light formed irradiates water body chlorophyll by optical window and produces fluorescence, and the fluorescence be reflected back is detected by the photodetector one in signal processing module after the transmission of dichroic light splitting piece.The fraction light beam that light source module sends is transmitted into through dichroic light splitting piece the reference signal that in referrer module, photodetector two changes as evaluation energy of light source.
Concrete technical scheme is as follows:
A kind of bifocal path technique coastal waters chlorophyllogen position monitoring method, based on Fluorometric assay principle, by directly measuring fluorescence intensity on-line monitoring chlorophyll α concentration; Concrete grammar is:
(1) excitation source in light source module adopts the blue LED of superbright;
(2) double light path test macro is adopted, the parallel light that LED light source is launched is divided into two-beam after inciding the dichroic light splitting piece installed through 45 ° of directions, a branch of through the dichroic light splitting piece reflection detection light at a right angle with incident light and a branch of reference light parallel with incident light;
(3) dichroic light splitting piece is that 45 ° of directions are installed, the light of reflection 400-480nm wavelength the light of transmission 500-700nm, ensure reflect detection light and make it impinge perpendicularly on water body, the fluorescence excited is received by photodetector one through light splitting piece, the incident light detecting fluorescence and LED light source is rectangular, with detection parallel light;
(4) light is detected perpendicular through condenser lens one and optical quartz window, become collimated light and be irradiated into water sample, the chlorophyll fluorescence sent that is stimulated is received by photodetector one through optical quartz window, condenser lens one, dichroic light splitting piece and condenser lens two again;
(5) the dichroic light splitting piece of light in measurement module that light source module sends is divided into two-beam, and a branch of reference light is wherein detected by photodetector two, then is processed by data acquisition unit;
(6) control module and data acquisition unit control light source luminescent, and tested water body chlorophyll is stimulated emitting fluorescence; The fluorescence received is converted to corresponding electric signal by photodetector one, and data processing unit processes the electric signal that photodetector one is changed again, and calculates the content of tested water body chlorophyll a, sends data to control module simultaneously and shows;
(7) data processing unit is calculated the content of tested water body chlorophyll a according to following formula by the fluorescence signal gathered:
C＝a×(E-F)
Wherein, C is tested water body algae chlorophyll-a concentration, and unit is mg/L; E is the fluorescence intensity being positioned at characteristic center wavelength 685nm place calculated; A is linearly dependent coefficient; F is the compensation numerical value correcting yellow substance CDOM in tested water body, compensates numerical value F and is stored in control treatment inside modules.
After the pre-service of tested water body fluorescent signal data, through system mathematic model analyzing and processing, calculate fluorescence intensity E, and according to being stored in the offset value value coefficient F of yellow substance CDOM of data processing module inside in conjunction with on-the-spot CDOM measured value, carry out chlorophyll fluorescence intensity correction, eliminate the interference of other materials that fluoresce of tested water body.Chlorophyll fluorescence signal data is again by after the drift of system mathematic model inner light source, partial least square method scheduling algorithm analyzing and processing, calculate the linearly dependent coefficient a between correction of fluorescence intensity (E-F) and chlorophyll concentration C, the last content being drawn tested water body chlorophyll a by retrieving concentration algorithm, sends data to control module simultaneously and shows.
The present invention further discloses a kind of device realizing described method, comprise signal processing module, photodetector one, condenser lens two, light source, photodetector two, dichroic light splitting piece, condenser lens one, quartzy optical window, shell, shell and front end main body adopt silicon bi-layer seal with elastometic washer, light source and photodetector two are all arranged in the main body of front end with holder, the below of front end main body is quartzy optical window, be water sample pond below quartz optical window, condenser lens one is arranged on the top of quartzy optical window; Dichroic light splitting piece adopts 45 ° of directions to be arranged on above condenser lens one, light source and photodetector two lay respectively at dichroic light splitting piece both sides, and the LED of light source module and photodetector two parallel concentric, ensure that the light that LED light source sends is divided into detection light and this two-beam of reference light after dichroic light splitting piece, photodetector one is fixed on above condenser lens two, and condenser lens two is fixed on the top of dichroic light splitting piece; Signal processing module is connected with photodetector two with photodetector one, light source module respectively; Reference light is detected by photodetector two, and detection light line focus lens one gather water sample pond, and the fluorescence line focus lens one excited in water sample pond and condenser lens two are received by photodetector one; Signal processing module arranges RS232 digital simulation output port, and connecting system uses together with thermometer, degree of depth instrument, data acquisition unit or other parameter sensors etc., setting changing gain coefficient, data average time and calibration factor in the controller.
Dichroic light splitting piece thickness is 1.2mm.
Advantage of the present invention is as follows:
The present invention adopts the blue LED of superbright as excitation source, and have luminous intensity high, volume is little, the feature of long service life.
The present invention adopts double light path test macro, and the light that light source sends is divided into detection light and reference light through dichroic light splitting piece, and detection optical excitation fluorescence, reference light is detected by photodetector two.The method eliminates light source fluctuation and light source and to decay the impact brought, and improves system stability and accuracy.
Fluorescence method mathematical model of the present invention, by the linearly dependent coefficient calculated, adds yellow substance CDOM compensate function, can correct yellow substance CDOM in tested water body and, on the impact of the fluorescence signal gathered, accurately calculate tested water body chlorophyll concentration.
Bifocal path technique coastal waters chlorophyllogen level monitoring system of the present invention and method volume little, lightweight, easy to carry, power consumption is little, accuracy and highly sensitive, is applicable to on-line monitoring.
Technique effect of the present invention: bifocal path technique coastal waters chlorophyllogen level monitoring system of the present invention and method can on-the-spot in real time, fast, large area monitoring water body Determination of Chlorophyll concentration, and lightweight, volume is little, accuracy and highly sensitive.Compared with the chlorophyllous instrument of traditional detection, the measurement of quick real-time continuous can be realized, be more convenient for carrying, use simple to operate, effectively decrease and produce and measure cost, applied range, can be used for the key areas such as marine environment investigation, mariculture, contamination index's appraisal and red tide monitoring, is beneficial to and realizes industrialization.
Accompanying drawing explanation
Fig. 1 is the structural representation of device of the present invention, wherein 1, signal processing module, 2, photodetector one, 3, condenser lens two, 4, light source, 5, photodetector two, 6, dichroic light splitting piece, 7, condenser lens one, 8, quartzy optical window, 9, shell; 10, front end main body, 11, water sample pond.
Fig. 2 is the workflow schematic diagram of present system device;
Fig. 3 is the Data Management Analysis process schematic of bifocal path technique coastal waters chlorophyllogen level monitoring system model.
Embodiment
The present invention by directly measure water body Determination of Chlorophyll be excited discharge fluorescence intensity detect chlorophyll concentration, primarily of light source module, measurement module, referrer module and signal processing module four part composition.
As shown in Figure 1, device of the present invention comprises signal processing module 1, photodetector 1, condenser lens 23, light source 4, photodetector 25, dichroic light splitting piece 6, condenser lens 1, quartzy optical window 8, shell 9, shell 9 and front end main body 10 adopt silicon bi-layer seal with elastometic washer, light source 4 and photodetector 25 are all arranged in the main body of front end with holder, the below of front end main body 10 is quartzy optical window 8, be water sample pond 11 below quartz optical window 8, condenser lens 1 arranges the top of quartzy optical window 8; Dichroic light splitting piece 6 adopts 45 ° of directions to be arranged on above condenser lens 1, light source 4 and photodetector 25 lay respectively at dichroic light splitting piece 6 both sides, and the LED of light source module 4 and photodetector 25 parallel concentric, ensure that the light that LED light source 4 sends is divided into detection light and this two-beam of reference light after dichroic light splitting piece 6, photodetector 1 is fixed on above condenser lens 23, and condenser lens 23 is fixed on the top of dichroic light splitting piece 6; Signal processing module 1 is connected with photodetector 25 with photodetector 1, light source module 4 respectively; Reference light is detected by photodetector 25, and detection light line focus lens 1 gather water sample pond 11, and the fluorescence line focus lens 1 excited in water sample pond and condenser lens 23 are received by photodetector 1; Signal processing module 1 arranges RS232 digital simulation output port, and connecting system uses together with thermometer, degree of depth instrument, data acquisition unit or other parameter sensors etc., setting changing gain coefficient, data average time and calibration factor in the controller.
Dichroic light splitting piece 6 thickness is 1.2mm.
As shown in Figure 2, when instrument of the present invention is started working, system program first completes initialization, then the control module in signal processing module and data acquisition unit control LED light source and export energy and launch blue light, because the present invention adopts double light path test macro, the parallel light that LED light source is launched is divided into two-beam after inciding the dichroic light splitting piece installed in 45 ° of directions, a branch of is detection light at a right angle with incident light after the reflection of dichroic light splitting piece, and another bundle is the reference light of incident light after the transmission of dichroic light splitting piece.Wherein, reference light is detected by photodetector two, then processed by the data acquisition unit in signal processing module, with the impact removing system major part parasitic light, improves system stability and accuracy.Detect light perpendicular through condenser lens one in the prefocusing of optical quartz window, become collimated light and be irradiated into water sample Determination of Chlorophyll, chlorophyll is stimulated injection fluorescence, the fluorescence of reflection is collected by same condenser lens one through optical quartz window again, by photodetector one is received after focusing on coupling through dichroic light splitting piece and condenser lens two.Reflected fluorescent light, before being accepted by photodetector one, also needs the light first being departed from specific wavelength 685nm by Red lightscreening plate filtering, makes the light signal entering photodetector one be all chlorophyll fluorescence signal, removes external stray light impact.The fluorescence signal received is converted to corresponding electric signal by photodetector one, data processing unit again to photodetector one change electric signal and process, and calculate the content of tested water body chlorophyll a, send data to control module simultaneously and show.
The data processing centre (DPC) of present system is single-chip microcomputer, and photodetector one is converted into potential pulse fluorescence signal, then after being exported by integrator by A/D converter converting transmission to single-chip microcomputer, be used for the collection of paired data, calculating and calibration etc.
Instrument of the present invention has RS232 digital simulation output port, can use by connecting system together with thermometer, degree of depth instrument, data acquisition unit and other parameter sensors etc.In the controller can setting changing gain coefficient, data average time and calibration factor etc.
As shown in Figure 3, the fluorescence that the present invention is excited to reflect by measurement water body Determination of Chlorophyll detects chlorophyll concentration, the Data Management Analysis step of the system model of this process as shown in Figure 3, control module controls light source luminescent and obtains reflected fluorescent light, data acquisition is carried out by photodetector one by data acquisition unit, the data collected are carried out pre-service by data processing unit again, and pre-service denoising is by level and smooth, signal averaging scheduling algorithm.Pretreated data finally calculate chlorophyll fluorescence related coefficient by system mathematic model analyzing and processing, the Data Analysis Services algorithm of system mathematic model mainly contains light source drift calibration, partial least square method, and according to being stored in the offset value value coefficient of yellow substance CDOM of data processing module inside in conjunction with on-the-spot CDOM measured value, carry out chlorophyll fluorescence intensity correction, the last content being drawn tested coastal waters chlorophyll a by retrieving concentration algorithm, sends data to control module simultaneously and shows.
Instrument calibration
Bifocal path technique coastal waters chlorophyllogen level monitoring system device of the present invention can be immersed in the water work completely, also can be arranged in pipeline and use.When using present system to test, first determine that sensor has been energized and has started normal work, if instrument normally works, time test pure water (not containing yellow substance CDOM), reading should be zero.Inaccurate for preventing directly testing water sample, can first calibrate with pure water test.Further calibration can be dissolved in chlorophyll α in 90% acetone and make the titer that concentration is 10,20,100 and 200 μ g/L respectively, and titer is placed in glass container, and instrument distance container bottom at least 8-10cm is tested, and obtains calibration factor.After instrument calibration, can be immersed in tested water body completely and measure, the control module of system controls light source luminescent and excites water body Determination of Chlorophyll to obtain reflected fluorescent light, data acquisition is carried out by photodetector one again by data acquisition unit, and the data of collection are carried out pre-service, then calculated the content of tested water body chlorophyll a by data processing unit, send data to control module simultaneously and show.It is little that native system structural design possesses volume, low in energy consumption, simple to operate, be convenient to boat-carrying, system adopts double photo paths system, has deducted most of interference parasitic light with reference light, and by the offset value value coefficient of the yellow substance CDOM that is stored in data processing module inside in conjunction with on-the-spot CDOM measured value, carry out chlorophyll concentration correction, make native system detect chlorophyll concentration reliable and stable.
The signal processing module adopted in the present invention, photodetector one, condenser lens two, light source, photodetector are second-class all can adopt commercial products.
Claims (3)
1. a bifocal path technique coastal waters chlorophyllogen position monitoring method, is characterized in that: based on Fluorometric assay principle, by directly measuring fluorescence intensity on-line monitoring chlorophyll α concentration; Concrete grammar is:
(1) excitation source in light source module adopts the blue LED of superbright;
(2) double light path test macro is adopted, the parallel light that LED light source is launched is divided into two-beam after inciding the dichroic light splitting piece installed in 45 ° of directions, a branch of is through the dichroic light splitting piece reflection detection light at a right angle with incident light, the reference light that another Shu Weiyu incident light is parallel;
(3) dichroic light splitting piece is that 45 ° of directions are installed, the light of reflection 400-480nm wavelength the light of transmission 500-700nm, ensure reflect detection light and make it impinge perpendicularly on water body, the fluorescence excited is received by photodetector one through light splitting piece, the incident light detecting fluorescence and LED light source is rectangular, with detection parallel light;
(4) light is detected perpendicular through condenser lens one and optical quartz window, become collimated light and be irradiated into water sample, the chlorophyll fluorescence sent that is stimulated is received by photodetector one through optical quartz window, condenser lens one, dichroic light splitting piece and condenser lens two again;
(5) reference light is detected by photodetector two, then is processed by data acquisition unit;
(6) control module and data acquisition unit control light source luminescent, and tested water body chlorophyll is stimulated emitting fluorescence; The fluorescence received is converted to corresponding electric signal by photodetector one, and data processing unit processes the electric signal that photodetector one is changed again, and calculates the content of tested water body chlorophyll a, sends data to control module simultaneously and shows;
(7) data processing unit is calculated the content of tested water body chlorophyll a according to following formula by the fluorescence signal gathered:
C＝a×(E-F)
Wherein, C is tested water body algae chlorophyll-a concentration, and unit is mg/L; E is the fluorescence intensity being positioned at characteristic center wavelength 685nm place calculated; A is linearly dependent coefficient; F is the compensation numerical value correcting yellow substance CDOM in tested water body, compensates numerical value F and is stored in control treatment inside modules.
2. one kind realizes the device of method described in claim 1, it is characterized in that: comprise signal processing module, photodetector one, condenser lens two, light source, photodetector two, dichroic light splitting piece, condenser lens one, quartzy optical window, shell, shell and front end main body adopt silicon bi-layer seal with elastometic washer, light source and photodetector two are all arranged in the main body of front end with holder, the below of front end main body is quartzy optical window, be water sample pond below quartz optical window, condenser lens one is arranged on the top of quartzy optical window; Dichroic light splitting piece adopts 45 ° of directions to be arranged on above condenser lens one, light source and photodetector two lay respectively at dichroic light splitting piece both sides, and the LED of light source module and photodetector two parallel concentric, ensure that the light that LED light source sends is divided into detection light and this two-beam of reference light after dichroic light splitting piece, photodetector one is fixed on above condenser lens two, and condenser lens two is fixed on the top of dichroic light splitting piece; Signal processing module is connected with photodetector two with photodetector one, light source module respectively; Reference light is detected by photodetector two, and detection light line focus lens one gather water sample pond, and the fluorescence line focus lens one excited in water sample pond and condenser lens two are received by photodetector one; Signal processing module arranges RS232 digital simulation output port, and connecting system uses together with thermometer, degree of depth instrument, data acquisition unit or other parameter sensors, sets in the controller and changes gain coefficient, data average time and calibration factor.
3. device according to claim 2, is characterized in that: dichroic light splitting piece thickness is 1.2mm.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954192A (en) * | 2016-07-20 | 2016-09-21 | 中国科学院烟台海岸带研究所 | Online dual-light-path water environment measurement device based on spectral measurement technology |
| CN106932373A (en) * | 2017-04-01 | 2017-07-07 | 燕山大学 | Total organic carbon optics home position sensing |
| CN107340237A (en) * | 2017-09-08 | 2017-11-10 | 中清盈创(深圳)科技有限公司 | Water quality on-line monitoring device based on light emitting diode |
| CN107543800A (en) * | 2017-10-10 | 2018-01-05 | 安徽英凯环境技术有限公司 | A kind of Light Source Compensation update the system |
| CN108414477A (en) * | 2018-01-12 | 2018-08-17 | 山东省科学院海洋仪器仪表研究所 | Seawater chlorophyll a, phycocyanin and phycoerythrin parameter measuring apparatus and method |
| CN109682787A (en) * | 2019-02-28 | 2019-04-26 | 中国科学院烟台海岸带研究所 | A kind of phase demodulating formula petroleum hydrocarbon class pollutant identification method and device |
| CN110426359A (en) * | 2019-08-09 | 2019-11-08 | 山东省科学院海洋仪器仪表研究所 | A kind of detection device and method of concentration self-adapting seawater total nitrogen/total phosphorus |
| CN112129716A (en) * | 2020-08-25 | 2020-12-25 | 江苏大学 | Green plant identification probe with adjustable sensitivity |
| CN112577900A (en) * | 2020-11-02 | 2021-03-30 | 青岛光流软件技术有限公司 | Water body ecological environment parameter scanning and monitoring system based on ocean optics |
| EP3943918A1 (en) | 2020-07-22 | 2022-01-26 | Universidade do Minho | Device for identification and quantification of phytoplankton, methods and uses thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060121614A1 (en) * | 2001-09-12 | 2006-06-08 | Hitachi, Ltd. | Multichannel fluorosensor |
| CN101413893A (en) * | 2008-11-27 | 2009-04-22 | 吉林大学 | Plant chlorophyll measuring instrument and chlorophyll measuring method |
| CN103105284A (en) * | 2013-01-14 | 2013-05-15 | 中国科学院光电技术研究所 | Lithography machine illuminating system optical module transmittance measuring device and method |
| CN103472045A (en) * | 2013-09-22 | 2013-12-25 | 王雅娜 | Quick on-line water body fluorescence meter |
| CN104990902A (en) * | 2015-06-24 | 2015-10-21 | 石家庄经济学院 | Plant chlorophyll fluorescence detection device based on LED |
-
2016
- 2016-02-20 CN CN201610093590.6A patent/CN105548128A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060121614A1 (en) * | 2001-09-12 | 2006-06-08 | Hitachi, Ltd. | Multichannel fluorosensor |
| CN101413893A (en) * | 2008-11-27 | 2009-04-22 | 吉林大学 | Plant chlorophyll measuring instrument and chlorophyll measuring method |
| CN103105284A (en) * | 2013-01-14 | 2013-05-15 | 中国科学院光电技术研究所 | Lithography machine illuminating system optical module transmittance measuring device and method |
| CN103472045A (en) * | 2013-09-22 | 2013-12-25 | 王雅娜 | Quick on-line water body fluorescence meter |
| CN104990902A (en) * | 2015-06-24 | 2015-10-21 | 石家庄经济学院 | Plant chlorophyll fluorescence detection device based on LED |
Non-Patent Citations (1)
| Title |
|---|
| 夏达英等: "海水叶绿素a现场测量仪研究", 《海洋与湖沼》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105954192A (en) * | 2016-07-20 | 2016-09-21 | 中国科学院烟台海岸带研究所 | Online dual-light-path water environment measurement device based on spectral measurement technology |
| CN105954192B (en) * | 2016-07-20 | 2019-07-30 | 中国科学院烟台海岸带研究所 | A kind of double light path water body environment on-line measurement device based on spectral measurement methods |
| CN106932373A (en) * | 2017-04-01 | 2017-07-07 | 燕山大学 | Total organic carbon optics home position sensing |
| CN106932373B (en) * | 2017-04-01 | 2023-09-29 | 燕山大学 | Seawater total organic carbon optical in-situ sensor |
| CN107340237A (en) * | 2017-09-08 | 2017-11-10 | 中清盈创(深圳)科技有限公司 | Water quality on-line monitoring device based on light emitting diode |
| CN107543800A (en) * | 2017-10-10 | 2018-01-05 | 安徽英凯环境技术有限公司 | A kind of Light Source Compensation update the system |
| CN108414477A (en) * | 2018-01-12 | 2018-08-17 | 山东省科学院海洋仪器仪表研究所 | Seawater chlorophyll a, phycocyanin and phycoerythrin parameter measuring apparatus and method |
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| CN110426359A (en) * | 2019-08-09 | 2019-11-08 | 山东省科学院海洋仪器仪表研究所 | A kind of detection device and method of concentration self-adapting seawater total nitrogen/total phosphorus |
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Application publication date: 20160504 |