CN101403695A - Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration - Google Patents
Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration Download PDFInfo
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- CN101403695A CN101403695A CNA2008101529657A CN200810152965A CN101403695A CN 101403695 A CN101403695 A CN 101403695A CN A2008101529657 A CNA2008101529657 A CN A2008101529657A CN 200810152965 A CN200810152965 A CN 200810152965A CN 101403695 A CN101403695 A CN 101403695A
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Abstract
The invention relates to a fluorometer used for online measuring the concentration of water chlorophyll quickly, in particular to an underwater fluorometer, belonging to the water quality analysis field; wherein, the underwater fluorometer is used for the in-situ quick measurement of the chlorophyll a, thus obtaining the real-time data of a water body. The technical proposal adopted by the fluorometer is that the fluorometer comprises an LED light source which carries out output by a turning mirror, and a postpositive photodiode used for receiving the fluorescence by a condensation lens group; the output of the postpositive photodiode is sequentially output by a signal automatic gain adjustment and signal collecting circuit, a control and signal processing module, a serial port and finally an output cable to a PC or a data collection system; the signal automatic gain adjustment and signal collecting circuit, the control and signal processing module and an RS-232 serial port are arranged on a circuit board in the fluorometer; and the circuit board in the fluorometer is also provided with a light source driving circuit which is used for driving the LED light source. The fluorometer is mainly applied to the water quality analysis.
Description
Technical field
The present invention relates to the water analysis field, specifically relate to fluorescence instrument for fast on-line measurement of water body chlorophyll concentration.
Background technology
In recent years, water crises such as Taihu Lake, Wuhu appear in China year after year, because blue green algae continues outburst in water body, make that phytoplankton increases severely in the water, and the water oxygen branch exhausts, and water body presents the large tracts of land anaerobic condition, organism in water so mass mortality.Because the quick growth of algae in the water, green alga is piled up on the water surface, causes the current difficulty, and water body can't carry out air with the external world and exchange, and the stench that becomes has day by day all brought great harm for people's production, life and life and health.
At present the relevant monitoring station and the Environmental Research Institute of domestic each lake region reservoir are mostly studied the eutrophication degree of water body, comprise single index such as chemical oxygen demand (COD), total nitrogen, total phosphorus, chlorophyll a content and by the eutrophication evaluation of the aggregative index of certain Model Calculation.But its assay method mostly is and brings to the laboratory after the sampling and analyze, or is monitoring and studying artificial the setting under the condition of culture.The former is owing to exist certain hysteresis quality and the error in the experimentation, and accuracy is relatively poor; Then because be ideal model, also there is certain gap in the latter with current conditions, and repeatability is difficult to guarantee.
The chlorophyll-a concentration of water body all is one of important parameter of estimating the lake eutrophication degree all the time.Domestic most this type of Monitoring Data all is to be finished by traditional laboratory method at present.The deficiency of laboratory method is very tangible.Its whole process comprises water sample is taken to the laboratory, and chlorophyll is extracted in the phytoplankton body, monitors with spectrophotometric method at last, and workload is very big.And, obtain wrong data at last probably owing to disturbing factor in whole process is a lot.
Summary of the invention
For overcoming the deficiencies in the prior art, the object of the invention is to provide a kind of fluorescent instrument under water, is used for the fast-field evaluation of chlorophyll a, thereby can obtain the real time data of water body.
The technical solution used in the present invention is that fluorescence instrument for fast on-line measurement of water body chlorophyll concentration comprises:
Be used for lasing led light source, led light source is exported through deviation mirror;
Be used for rearmounted photodiode by the fluorescence of collector lens group of received chlorophyll Stimulated Light irradiation generation;
Rearmounted photodiode outputs to microcontroller by signal circuit, and microcontroller outputs to PC or data acquisition system (DAS) by the RS-232 serial ports;
Signal circuit comprises wave filter, amplifier, automatic gain adjustment circuit and the difference engine that links to each other successively;
Described signal circuit, microcontroller, RS-232 serial port setting also are provided with the light source driving circuit that is used for the driving LED light source on the instrument internal circuit board on the instrument internal circuit board.
Microcontroller is single-chip microcomputer or frequency divider.
Led light source is that wavelength is a 350nm ultraviolet LED light source.
The collector lens group comprises an optical filter that is used to receive the wavelength 620nm-715nm of fluorescence.
Led light source, deviation mirror, collector lens group, rearmounted photodiode, instrument internal circuit board place in the stainless steel cylinder, stainless steel column diameter 22mm, length 120mm, deviation mirror, collector lens group and rearmounted photodiode are arranged on the same end of stainless steel cylinder.
The present invention can bring following technique effect:
1) the maximum feature of highly sensitive fluorometry is to have very high absolute sensitivity.Be that other spectroscopic methodology is inaccessiable under certain conditions.Its minimum detectability is between 10-6～10-9 order of magnitude at massfraction, than highly sensitive 2～4 orders of magnitude of UV-VIS spectrophotometry.Because chlorophyll specifically has specificity fluorescent, can improve sensitivity for analysis by increasing incident intensity and reducing spectral bandwidth.2) the high fluorescence of selectivity is because after material molecule absorbs the radiation energy of certain wavelength, the light that sends when the ground state transition from excited state.Yet can might not produce fluorescence by light absorbing material, and under the exciting of certain wavelength light, the wavelength that can produce the material institute emitting fluorescence of fluorescence is not quite similar also.According to the excitation spectrum and the emission spectrographic analysis of chlorophyll fluorescence, have only and select the exciting light of certain wavelength just can obtain chlorophyllous fluorescence, so the fluorescence signal that this method obtains can be relevant with chlorophyll content exactly.3) method is easy fast, sample size is few.Because fluorescence phenomenon takes place rapidly, the condition simple controllable so measure rapidly, is convenient to realize on-site measurement.Compare the spectrophotometry chlorophyll a, extract concentrate from a large amount of water samples, processing time while is very long, needs a large amount of organic solvents, and this method really can realize field quick detection.
Description of drawings
Accompanying drawing is general structure of the present invention and principle of work synoptic diagram, among the figure: the 1st, led light source, the 2nd, deviation mirror, the 3rd, collector lens group, the 4th, rearmounted photodiode, the 5th, instrument internal circuit board, the 6th, output cable.
Embodiment
Most phytoplankton has a large amount of basic pigments, comes to give chlorophyll a the NE BY ENERGY TRANSFER that absorbs, though these pigments also can send very strong fluorescence, does not have the fluorescent characteristic of certain rule.And main fluorescence spectral characteristic is determined by chlorophyll a.In case so we when the fluorescence spectrum that will monitor is set at the feature of chlorophyll a, just can obtain the content of chlorophyll a more exactly.
The peak value of two tangible absorbing bands of chlorophyll a is respectively near 420nm and 665nm, and near the absorption band broad 420nm.The existence of phytoplankton body inner chlorophyll has determined the absorption Spectrum characteristic of phytoplankton basically like this, and the peak wavelength of two strong absorption bands of phytoplankton is basically near 440nm and 670nm.And the photoluminescence peak wavelength of phytoplankton body inner chlorophyll a is near 685nm.
Phytoplankton not only demonstrates chlorophyllous absorption characteristic, and optically equivalence is a particulate matter, plays a part scattered light.Chlorophyllous absorption of phytoplankton and scattering properties quite stable, the shape of its absorption spectrum and back scattering spectrum under any circumstance all are invariable, and its amplitude is the function of chlorophyll content.This has also improved reliability for fluorometry.
Based on this, this patent adopts fluorescence detection method, with the ultraviolet LED of peak wavelength 350nm as exciting light, adopt the optical filter of 620nm-715nm to receive chlorophyll fluorescence, instrument internal is equipped with light source driving circuit, signal circuit, possesses optical detection, signal analysis and data-transformation facility.Signal circuit comprises wave filter, amplifier, automatic gain adjustment circuit and difference engine.Instrument is integrated in light source, deviation mirror, collector lens group, rearmounted broadcasting and TV diode, instrument internal circuit board etc. in the stainless steel cylinder, and its size has only diameter 22mm, and length 120mm has really realized high-performance, miniaturization monitoring system.Further describe the present invention below in conjunction with accompanying drawing.
Instrument internal is equipped with the led light source 1 that peak wavelength is 350nm, its exciting light that sends enters current water through deviation mirror 2, the chlorophyll that contains in the water body produces the fluorescence of 620nm-715nm, received by rearmounted photodiode 4 by collector lens group 3 backs, the height of chlorophyll content has positive correlation with photosignal output.Instrument internal circuit board 5 has embedded light source driving circuit, signal automatic gain adjustment and has been used for the microcontroller of signals collecting, signal analysis, signal Processing, signal controlling, and exports the digital signal of chlorophyll content by the RS-232 serial ports.Be connected with PC or data acquisition system (DAS) by output cable 6 at last.Microcontroller of the present invention adopts single-chip microcomputer, and frequency divider is also tested, and both effects are identical.
Eutrophy degree in the chlorophyll fluorescence analyzing water body of this patent employing high sensitivity, high accuracy.Be different from spectrophotometric method and laboratory fluorescence analysis method, only need by measuring that chlorophyll fluorescence intensity promptly can be used for estimating in the water body because algae reproduction and phytoplankton increase peculiar smell in the water that causes, algae is piled up and relevant water body quality such as fish kills.Do not need reagent, can directly apply to quick on-the-spot water quality detection monitoring after certain demarcation.
Claims (5)
1. a fluorescence instrument for fast on-line measurement of water body chlorophyll concentration is characterized in that, comprising:
Be used for lasing led light source, led light source is exported through deviation mirror;
Be used for rearmounted photodiode by the fluorescence of collector lens group of received chlorophyll Stimulated Light irradiation generation;
Rearmounted photodiode outputs to microcontroller by signal circuit, and microcontroller outputs to PC or data acquisition system (DAS) by the RS-232 serial ports;
Signal circuit comprises wave filter, amplifier, automatic gain adjustment circuit and the difference engine that links to each other successively;
Described signal circuit, microcontroller, RS-232 serial port setting also are provided with the light source driving circuit that is used for the driving LED light source on the instrument internal circuit board on the instrument internal circuit board.
2. a kind of fluorescence instrument for fast on-line measurement of water body chlorophyll concentration according to claim 1 is characterized in that, microcontroller is single-chip microcomputer or frequency divider.
3. a kind of fluorescence instrument for fast on-line measurement of water body chlorophyll concentration according to claim 1 is characterized in that, led light source is that wavelength is a 350nm ultraviolet LED light source.
4. a kind of fluorescence instrument for fast on-line measurement of water body chlorophyll concentration according to claim 1 is characterized in that, the collector lens group comprises an optical filter that is used to receive the wavelength 620nm-715nm of fluorescence.
5. a kind of fluorescence instrument for fast on-line measurement of water body chlorophyll concentration according to claim 1, it is characterized in that, led light source, deviation mirror, collector lens group, rearmounted photodiode, instrument internal circuit board place in the stainless steel cylinder, stainless steel column diameter 22mm, length 120mm, deviation mirror, collector lens group and rearmounted photodiode are arranged on the same end of stainless steel cylinder.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101529657A CN101403695A (en) | 2008-11-12 | 2008-11-12 | Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101529657A CN101403695A (en) | 2008-11-12 | 2008-11-12 | Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration |
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| CN101403695A true CN101403695A (en) | 2009-04-08 |
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| CNA2008101529657A Pending CN101403695A (en) | 2008-11-12 | 2008-11-12 | Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102564967A (en) * | 2010-12-13 | 2012-07-11 | 依诺特生物能量控股公司 | Chlorophyll fluorescent spectroscopy analyzer and method for measuring chlorophyll concentration |
| CN102749313A (en) * | 2012-07-05 | 2012-10-24 | 同济大学 | Remote measuring system for vertical distribution concentration of chlorophyll in seawater |
| CN102928390A (en) * | 2012-09-27 | 2013-02-13 | 中国农业大学 | On-line detection method and device for chlorophyll concentration in water body based on two detectors |
| CN103278482A (en) * | 2013-04-28 | 2013-09-04 | 燕山大学 | Dual-probe type optical detection device for detecting chlorophyll-a in water |
| CN103472045A (en) * | 2013-09-22 | 2013-12-25 | 王雅娜 | Quick on-line water body fluorescence meter |
| CN105203481A (en) * | 2015-09-21 | 2015-12-30 | 上海光谱仪器有限公司 | Spectrophotometer data collecting system and method based on pulse xenon lamp |
| CN112881349A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Optical probe applied to underwater fluorescence detection |
-
2008
- 2008-11-12 CN CNA2008101529657A patent/CN101403695A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102564967A (en) * | 2010-12-13 | 2012-07-11 | 依诺特生物能量控股公司 | Chlorophyll fluorescent spectroscopy analyzer and method for measuring chlorophyll concentration |
| CN102749313A (en) * | 2012-07-05 | 2012-10-24 | 同济大学 | Remote measuring system for vertical distribution concentration of chlorophyll in seawater |
| CN102928390A (en) * | 2012-09-27 | 2013-02-13 | 中国农业大学 | On-line detection method and device for chlorophyll concentration in water body based on two detectors |
| CN102928390B (en) * | 2012-09-27 | 2015-04-08 | 中国农业大学 | On-line detection device for chlorophyll concentration in water body based on two detectors |
| CN103278482A (en) * | 2013-04-28 | 2013-09-04 | 燕山大学 | Dual-probe type optical detection device for detecting chlorophyll-a in water |
| CN103472045A (en) * | 2013-09-22 | 2013-12-25 | 王雅娜 | Quick on-line water body fluorescence meter |
| CN105203481A (en) * | 2015-09-21 | 2015-12-30 | 上海光谱仪器有限公司 | Spectrophotometer data collecting system and method based on pulse xenon lamp |
| CN112881349A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Optical probe applied to underwater fluorescence detection |
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