CN102147365A - Handheld bioluminescent detector and detection method - Google Patents
Handheld bioluminescent detector and detection method Download PDFInfo
- Publication number
- CN102147365A CN102147365A CN2010106074602A CN201010607460A CN102147365A CN 102147365 A CN102147365 A CN 102147365A CN 2010106074602 A CN2010106074602 A CN 2010106074602A CN 201010607460 A CN201010607460 A CN 201010607460A CN 102147365 A CN102147365 A CN 102147365A
- Authority
- CN
- China
- Prior art keywords
- module
- signal
- photomultiplier
- optical filter
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a handheld bioluminescent detector and a detection method. The handheld bioluminescent detector is characterized by comprising an optical detection module 1, a signal acquisition module 2, a signal processing module 3, a signal display and transmission module 4 and a power supply module 5. The detection method for the handheld bioluminescent detector is characterized by comprising the steps: converting bioluminescent signals to be detected into electric signals by the optical detection module 1, transmitting the electric signals to the signal processing module 3 by the signal acquisition module 2, displaying and transmitting the completed result by the signal processing module, and simultaneously providing feedback control quantity to the optical detection module 1 by the signal processing module. The power supply module 5 is used for supplying a proper working voltage to all the modules. By means of the detector and the detection method provided by the invention, the traditional problem that weak bioluminescent signals are difficult to detect by using a handheld instrument is solved effectively.
Description
Technical field
The present invention relates to a kind of hand-held bioluminescence detector and detection method.Detection method provided by the present invention and instrument can be used for the highly sensitive detection and the analysis of faint bioluminescence, belong to advanced and make and technical field of automation.
Background technology
Detection technique of fluorescence is a kind of detection technique commonly used in the analytical chemistry, and the fluorescence that reflects this substance characteristics that this technology utilizes some material (back is excited) to be induced carries out qualitative or quantitative test.At present, detection technique of fluorescence has been widely used in fields such as biology, analytical chemistry, exploration and environmental monitoring.In these fields, fluorescence and analysis and detecting instrument commonly used comprise: fluorescent microscope, confocal fluorescent microscope, fluorospectrophotometer and fluorescence spectrophotometer etc.Yet these instruments are function singleness often, and bulky, can't be portable, and this can't satisfy detecting in real time fast of the on-the-spot sample of outdoor complexity.Simultaneously, the bioluminescence signal in the environment is more complicated often: the light intensity of fluorescence changes greatly, the spectral range broad of fluorescence (the characteristic peak differences of different samples).
The photoelectric conversion module that fluorescence light intensity in the particular range of wavelengths is converted to electric signal is one of gordian technique that realizes hand-held fluoroscopic examination process.In recent years, the appearance successively of miniaturization photo-electricity switching device, this is comprising photoelectric tube (Phototube), photoelectric diode (Photo-Diode) etc.At present, the total number of bacteria detector that uses these photoelectric conversion modules to make hand-held is arranged, as the SystemSURE series of products employing photoelectric tube of Hygiena company.Yet photoelectric tube and photodiode dark current are bigger, and particularly its sensitivity is lower, and need the fluorescence signal of detection often fainter, have so just limited the detection performance and the usable range of instrument.(Photomultiplier Tube PMT) is a kind of high sensitivity that has, the light-detecting device of low noise and ultrafast time response to photomultiplier.Optics that the photomultiplier transit pipe jointing of miniaturization is suitable and electricity module make the fluorescence detecting system of hand-held become possibility.Yet, do not see the example that uses the miniaturization photo-electricity multiplier tube to make the hand-held fluorescence detecting system so far yet.The invention provides the handheld instrument that a kind of bioluminescence detects, the photomultiplier of this instrument integration of compactization, in conjunction with suitable optics and electricity module, the quick real-time fluorescence that satisfies the on-the-spot sample of outdoor complexity detects.
Summary of the invention
The purpose of this invention is to provide a kind of hand-held bioluminescence detector and detection method, described bioluminescence detection method can be used for the bioluminescence signal, and (luminous flux of fluorescence signal is at 1 skin lumen～1 milli lumen, the detection of the spectral range 300nm of bioluminescence signal～750nm).
The objective of the invention is to reach by following measure: described hand-held bioluminescence detector comprises optical detection module, signal acquisition module, signal processing module, transport module and power module.Integrated rotary-type optical filter in the optical detection module (covering 300nm～750nm scope) selects for use suitable optical filter to finish the filter task of fluorescence signal in the specific spectra scope by circuit control.Photomultiplier is selected suitable sensitivity through peripheral circuit, finishes opto-electronic conversion, and this fluorescence signal is changed to electric signal.Signal acquisition module and signal processing module are finished the collection of signal, filtering and Processing tasks, and the result fed back in the optical detection module, automatically control the detected parameters of optical detection module; Power module has multichannel output, and the operating voltage of above-mentioned each module is provided.
1. described rotary-type optical filter covers the 300-750nm wavelength coverage, and the response wave length scope of photomultiplier is consistent with the optical filter wavelength coverage, also is 300-750nm;
2. the sensitivity of photomultiplier is adjustable in 50A/ lumen-0.0001A/ lumen range;
3. signal acquisition module adopts resistance and low frequency filtering mode, by the signals collecting amplifying circuit of two-level concatenation structure, effectively gathers the current signal of skin peace magnitude;
4. described power module adopts MAX 856 or MAX 743 chips, provide respectively 5V and ± the voltage output of 12V, have multichannel output, be respectively applied for the operating voltage of signal processor and operational amplifier.
Detector of the present invention and detection method can solve the traditional problem that handheld instrument is difficult to detect hypofluorescence signal effectively.The detection step of described detector is:
(1) detected biological sample is placed in the sample cell in the optical detection module;
(2) select suitable optical filter by control circuit;
(3) photomultiplier is gathered the fluorescence signal of biological sample, and fluorescence signal is converted to current output signal;
(4) signal acquisition module realizes photomultiplier current signals and amplification;
(5) signal processing module has digital filtering function and feedback function, adopt single-chip microcomputer to finish reading in of signal and processing procedure in conjunction with modulus conversion chip, and the result is fed back to the optical detection module by another analog-digital chip, select and regulate photomultiplier detection sensitivity and gain for use with control output voltage, optical filter;
(6) show by signal at last and the control procedure and the testing result of transport module step display (5).
Description of drawings
Fig. 1 is the principle schematic of total number of bacteria pick-up unit structure;
Fig. 2 is the principle schematic of optical detection module;
Fig. 3 is the circuit diagram of signal acquisition module;
Fig. 4 is the circuit diagram of signal processing module.
Embodiment
At the demand of micro-atriphos (ATP) detection, the detecting instrument and the detection method that adopt the present invention to propose in conjunction with the accompanying drawings, illustrate specific embodiments according to the invention below.
Detecting instrument comprises that mainly optical detection module 1, signal acquisition module 2, signal processing module 3, signal show and transport module 4 and power module 5.Optical detection module 1 mainly comprises optical filter 8 and photomultiplier 9 two parts, and wherein photomultiplier PMT selects the H6780 photomultiplier of the loose company in Japanese shore for use.The responding range of this photomultiplier is between 300nm～750nm, and sensitivity is the 50A/ lumen.Optical detection module 1 is assembled in the seal chamber of being processed by metallic aluminum material 10, shields the influence of ambient light and electromagnetic interference (EMI) effectively, has improved detection sensitivity; During detection, biological sample is put into the sample cell 6 of optical detection module 1, by control motor 7 rotating wheels, choose suitable optical filter 8 (～600nm).Then, photomultiplier is gathered the fluorescence signal of biological sample, is converted into current output signal.Signal acquisition module 2 is used to realize that the PMT output current signal is gathered and amplification.Because it is extremely faint that low concentration ATP reaction discharges fluorescence signal, PMT effectively is output as the current signal of the pA～nA level of low frequency.Therefore signal acquisition module 2 adopts the signals collecting amplifying circuit based on sampling resistor 11 and low frequency filtering 12 modes, two-level concatenation structure, and wherein, the first order 11 enlargement factors are 1 * 10
8V/A, the second level 12 enlargement factors are 100 times.Signal processing module 3 adopts single-chip microcomputer STC89C5213 to finish reading in of signal and processing procedure in conjunction with modulus conversion chip MAX19715, analog-digital chip MAX53014 is used for output control voltage, and that finishes optical filter selects and regulate PMT module detection sensitivity and gain for use.Transport module 4 adopts the LCD MODULE YM12864J of the good apparent Electronics Co., Ltd. in Dalian, is used to show control procedure and testing result.Power module 5 adopts MAX856 and MAX743 chips, provide respectively 5V and ± the voltage output of 12V.
Claims (8)
1. a hand-held bioluminescence detector is characterized in that described detector comprises optical detection module, signal acquisition module, signal processing module, demonstration and transport module and power module; Integrated rotary-type optical filter in the optical detection module selects suitable optical filter to finish the task that leaches of fluorescence signal in the specific spectra scope by circuit control; Photomultiplier is selected suitable sensitivity through peripheral circuit, finishes opto-electronic conversion, and transfers this fluorescence signal to electric signal; Signal acquisition module and signal processing module are finished the collection of signal, filtering and processing, and the result fed back in optical module, automatically control the detected parameters of optical module; Power module has multichannel output, and the operating voltage of above-mentioned module is provided.
2. by the described detector of claim 1, it is characterized in that described rotary-type optical filter covers the 300-750nm wavelength coverage, the response wave length scope of photomultiplier is consistent with the optical filter wavelength coverage, also is 300-750nm.
3. by the described detector of claim 2, it is characterized in that the sensitivity of photomultiplier is adjustable in 50A/ lumen-0.0001A/ lumen range.
4. by the described detector of claim 1, it is characterized in that signal acquisition module adopts resistance and low frequency filtering mode,, effectively gather the current signal of skin peace magnitude by the signals collecting amplifying circuit of two-level concatenation structure.
5. by the described detector of claim 1, it is characterized in that first order resistance enlargement factor is 1 * 10
8V/A, second level enlargement factor is 100 times.
6. by the described detector of claim 1, it is characterized in that described power module adopts MAX856 or MAX 743 chips, provide respectively 5V and ± the voltage output of 12V, have multichannel output, be respectively applied for the operating voltage of signal processor and operational amplifier.
7. use the detection method of each described detector among the claim 1-6, it is characterized in that concrete steps are:
(1) detected biological sample is placed in the sample cell in the optical detection module;
(2) select suitable optical filter by control circuit;
(3) photomultiplier is gathered the fluorescence signal of biological sample, and fluorescence signal is converted to current output signal;
(4) signal acquisition module realizes photomultiplier current signals and amplification;
(5) signal processing module has digital filtering function and feedback function, adopt single-chip microcomputer to finish reading in of signal and processing procedure in conjunction with modulus conversion chip, and the result is fed back to the optical detection module by another analog-digital chip, select and regulate photomultiplier detection sensitivity and gain for use with control output voltage, optical filter;
(6) show by signal at last and the control procedure and the testing result of transport module step display (5).
8. by the described method of claim 7, when it is characterized in that detecting atriphos, 1. selecting photomultiplier for use is the H6780 photomultiplier of Japanese Bin Song company; 2. selecting the optical filter wavelength for use is 600nm; 3. the single-chip microcomputer of signal processing module employing is STC89C52; The analog-digital chip model that is used to export control voltage is MAX530, and the model of reading in the modulus conversion chip of processing procedure of finishing signal is MAX197.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106074602A CN102147365A (en) | 2010-12-22 | 2010-12-22 | Handheld bioluminescent detector and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106074602A CN102147365A (en) | 2010-12-22 | 2010-12-22 | Handheld bioluminescent detector and detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102147365A true CN102147365A (en) | 2011-08-10 |
Family
ID=44421740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010106074602A Pending CN102147365A (en) | 2010-12-22 | 2010-12-22 | Handheld bioluminescent detector and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102147365A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063642A (en) * | 2012-12-26 | 2013-04-24 | 西北农林科技大学 | Portable multi-index fluorescent biosensor device |
| CN103424716A (en) * | 2012-05-17 | 2013-12-04 | 安徽宏实光机电高科有限公司 | Lamp tube detection system based on application of color sorter |
| CN105203481A (en) * | 2015-09-21 | 2015-12-30 | 上海光谱仪器有限公司 | Spectrophotometer data collecting system and method based on pulse xenon lamp |
| CN106979941A (en) * | 2017-05-11 | 2017-07-25 | 陕西金士盾警用装备有限公司 | The fluorescence signal detection means and its detection method of a kind of fluorescence explosive detection instrument |
| CN107607514A (en) * | 2017-11-06 | 2018-01-19 | 合肥中科迪沃宁科技有限公司 | A kind of multichannel miRNA fast detectors |
| CN107632004A (en) * | 2017-10-24 | 2018-01-26 | 浙江大学宁波理工学院 | A kind of online atriphos detection means and its detection method |
| CN108507986A (en) * | 2018-03-17 | 2018-09-07 | 杨佳苗 | The discrete fluorescence spectrum of differential confocal and fluorescence lifetime detection method and device |
| CN108844930A (en) * | 2018-05-14 | 2018-11-20 | 杨佳苗 | It is divided the confocal discrete fluorescence spectrum of pupil and fluorescence lifetime detection method and device |
| TWI702397B (en) * | 2019-05-29 | 2020-08-21 | 麗寶大數據股份有限公司 | Fluorescent microscopic imaging device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435794A (en) * | 2002-01-30 | 2003-08-13 | 无锡市朗珈生物医学工程有限公司 | Bio-chip fluorescent detection scanning device with light filter rotary disk |
| US20050157304A1 (en) * | 2004-01-20 | 2005-07-21 | General Electric Company | Handheld device with a disposable element for chemical analysis of multiple analytes |
| CN1727879A (en) * | 2004-07-29 | 2006-02-01 | 中国科学院大连化学物理研究所 | Portable instrument for detecting pesticide residue through fluorescent probe |
| CN101038256A (en) * | 2007-04-03 | 2007-09-19 | 西安天隆科技有限公司 | Hand-held adenosine triphosphate fluorescent detecting instrument |
| CN101105455A (en) * | 2007-07-04 | 2008-01-16 | 四川大学 | Laser induction fluorescence detector |
-
2010
- 2010-12-22 CN CN2010106074602A patent/CN102147365A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435794A (en) * | 2002-01-30 | 2003-08-13 | 无锡市朗珈生物医学工程有限公司 | Bio-chip fluorescent detection scanning device with light filter rotary disk |
| US20050157304A1 (en) * | 2004-01-20 | 2005-07-21 | General Electric Company | Handheld device with a disposable element for chemical analysis of multiple analytes |
| CN1727879A (en) * | 2004-07-29 | 2006-02-01 | 中国科学院大连化学物理研究所 | Portable instrument for detecting pesticide residue through fluorescent probe |
| CN101038256A (en) * | 2007-04-03 | 2007-09-19 | 西安天隆科技有限公司 | Hand-held adenosine triphosphate fluorescent detecting instrument |
| CN101105455A (en) * | 2007-07-04 | 2008-01-16 | 四川大学 | Laser induction fluorescence detector |
Non-Patent Citations (1)
| Title |
|---|
| 周爱玉等: "手持式ATP生物荧光检测仪研制", 《传感技术学报》, vol. 21, no. 4, 30 April 2008 (2008-04-30), pages 543 - 546 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424716A (en) * | 2012-05-17 | 2013-12-04 | 安徽宏实光机电高科有限公司 | Lamp tube detection system based on application of color sorter |
| CN103063642A (en) * | 2012-12-26 | 2013-04-24 | 西北农林科技大学 | Portable multi-index fluorescent biosensor device |
| CN105203481A (en) * | 2015-09-21 | 2015-12-30 | 上海光谱仪器有限公司 | Spectrophotometer data collecting system and method based on pulse xenon lamp |
| CN106979941A (en) * | 2017-05-11 | 2017-07-25 | 陕西金士盾警用装备有限公司 | The fluorescence signal detection means and its detection method of a kind of fluorescence explosive detection instrument |
| CN106979941B (en) * | 2017-05-11 | 2023-03-14 | 陕西金士盾防务技术有限公司 | Fluorescent signal detection device and method for fluorescent explosive detector |
| CN107632004A (en) * | 2017-10-24 | 2018-01-26 | 浙江大学宁波理工学院 | A kind of online atriphos detection means and its detection method |
| CN107607514A (en) * | 2017-11-06 | 2018-01-19 | 合肥中科迪沃宁科技有限公司 | A kind of multichannel miRNA fast detectors |
| CN108507986A (en) * | 2018-03-17 | 2018-09-07 | 杨佳苗 | The discrete fluorescence spectrum of differential confocal and fluorescence lifetime detection method and device |
| CN108844930A (en) * | 2018-05-14 | 2018-11-20 | 杨佳苗 | It is divided the confocal discrete fluorescence spectrum of pupil and fluorescence lifetime detection method and device |
| TWI702397B (en) * | 2019-05-29 | 2020-08-21 | 麗寶大數據股份有限公司 | Fluorescent microscopic imaging device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102147365A (en) | Handheld bioluminescent detector and detection method | |
| CN104730054B (en) | A kind of integrated probe formula photoelectricity multi-parameter water-quality on-line measurement system | |
| US8004674B2 (en) | Data collection system and method for a flow cytometer | |
| CN103529006B (en) | A kind of Portable fluorescence based on micro-fluidic chip detection device | |
| CN202351177U (en) | Aflatoxin detector based on ultraviolet LED light source | |
| CN111308289A (en) | Partial discharge multispectral weak light detection device and method | |
| US7301158B1 (en) | Method and apparatus for measuring active fluorescence | |
| CN204556499U (en) | The multi-channel high-speed data acquisition and processing system of tuning diode absorption spectrum | |
| CN103424387A (en) | Fluorescence spectrum detection device for pesticide residue | |
| CN204101462U (en) | A kind of Raman spectrum water quality in-situ monitoring device | |
| CN101403695A (en) | Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration | |
| CN108872141B (en) | Near infrared spectrum analysis-based rapid nondestructive testing circuit, device and method for water content of rice leaves | |
| CN203310753U (en) | Multi-wavelength capillary electrophoresis fluorescence excitation device | |
| CN101598667A (en) | The multi-excitation multi-emission wavelength underwater in-situ organic matter fluorescence automatic detector | |
| CN204462021U (en) | Fluorescence analyser | |
| CN201607408U (en) | ATP fluorescence detection device | |
| CN107490432A (en) | A kind of Feebleness Light Signal Examining system | |
| CN106908426A (en) | Specified plant oil safe screen is checked and surveys method, apparatus and system | |
| CN104181133A (en) | Multi-wavelength capillary electrophoresis fluorescence excitation device | |
| CN206684048U (en) | A kind of minimal feeding instrument in double fluorescent emission faces | |
| CN204575538U (en) | A kind of laser-induced fluorescence (LIF) food detector | |
| CN205301156U (en) | Soil heavy metal detection device | |
| CN214011057U (en) | Underwater multi-wavelength backscatter and fluorescence monitoring probe | |
| CN204514810U (en) | Laser-induced fluorescence detection system | |
| Pan et al. | A submersible in-situ highly sensitive chlorophyll fluorescence detection system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110810 |