CN206684048U - A kind of minimal feeding instrument in double fluorescent emission faces - Google Patents
A kind of minimal feeding instrument in double fluorescent emission faces Download PDFInfo
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- CN206684048U CN206684048U CN201720484766.0U CN201720484766U CN206684048U CN 206684048 U CN206684048 U CN 206684048U CN 201720484766 U CN201720484766 U CN 201720484766U CN 206684048 U CN206684048 U CN 206684048U
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Abstract
A kind of minimal feeding instrument in double fluorescent emission faces, it belongs to the technical field of spectral detection.The detector includes main optical path, cuvette and two parallel legs, parallel legs and is mutually perpendicular to main optical path.A wave band light path in the device can detect the fluorescence intensity of internal standard substance, realize that Ratio-type detects, and have the advantages of detection operation is convenient, repeatability and accuracy are high.The detector is to assemble fluorescence respectively by the cuvette both sides perpendicular with excitation light path, and is realized by the optical filtering of different-waveband；Compared with the multiband fluorescence detection means in single fluorescent emission face, dichroic mirror is avoided in the system structure quantity is set, make fluorescence loss less, light path it is short, there is higher sensitivity and lower detectable limit, available for it is quantitative detect trace materials portable fluorescence detector device.
Description
Technical field
A kind of minimal feeding instrument in double fluorescent emission faces is the utility model is related to, it belongs to the technology neck of spectral detection
Domain.
Background technology
The internal standard method commonly used in analytical chemistry is a kind of indirect or relative calibration method.Certain group in determination sample is analyzed
During point content, a kind of internal standard substance is added to calibrate and eliminate fluctuation for testing conditions and to shadow caused by analysis result
Ring, to improve the degree of accuracy of analysis result, realize quantitative detection.It can simultaneously be detected by the use of double light path fluorescence detecting system and be used as ginseng
The fluorescence intensity of the fluorescence probe transmitting more associated than the fluorescence intensity and detected material of the transmitting of internal standard fluorescent dye, realizes ratio
Type detects, and can eliminate the excitation source lamp luminous intensity between different instruments and error caused by wavelength difference, make mass production
Instrument calibration simplify, improve accuracy rate and repeatability.Patent " a kind of small-sized double light path fluorescence detection device,
201620412985.3 ", the aggregated lens of fluorescence that cuvette one side is launched are converted into directional light, then be divided into two through dichroic mirror
The fluorescence of individual different-waveband, finally reach photodiode via optical filtering and collector lens.Use can low electricity in this device
Pressure starts, inexpensive general photodiode, realizes small-sized Ratio-type fluoroscopic examination.The dichroic mirror damage set due to the device
Part fluorescence intensity is consumed, and light path is longer, reduces the fluorescence intensity for injecting photodiode, it is difficult to meet relatively low detection pole
The trace materials testing requirements of limit.
The content of the invention
To solve problems of the prior art, the present invention provides a kind of minimal feeding in double fluorescent emission faces
Instrument, on the basis of the advantages of keeping the two waveband Ratio-type detection in single fluorescent emission face, it is not provided with dichroic mirror and fluorescence optically focused is saturating
Mirror, using the correspondence from cuvette and the fluorescence strategy for two faces collection, the two beam different-wavebands being parallel to each other, further improve
The sensitivity of instrument, reduce detectable limit.
The technical solution adopted by the present invention：A kind of minimal feeding instrument in double fluorescent emission faces, it includes power supply, data
Collection amplification system, automatically control with network transmission system, display device, it also include excite unit, the first detection unit and
Second detection unit；First detection unit is parallel with the second detection unit, excites unit to be mutually perpendicular to the first detection unit；Institute
State and excite unit to include excitation source, lamp condenser lens, light source filter and cuvette；First detection unit includes the
One fluorescence collector lens, the first fluorescence optical filtering, the first photodiode；Second detection unit includes the second fluorescence optically focused
Lens, the second fluorescence optical filtering, the second photodiode；Cuvette is in same with the first detection unit, the second detection unit
On straight line, the fluorescence for the opposite direction of the launch of two beams that cuvette emits respectively enters the first detection unit, the second detection list
Member；First photodiode, the second photodiode are electrically connected to data acquisition amplification system, automatically controlled and network transmission system
System and display device.
A kind of detection method of the minimal feeding instrument in double fluorescent emission faces：
（a）The light of excitation source transmitting is irradiated to cuvette via collector lens and optical filtering, and the detection in cuvette is glimmering
Light probe and reference internal standard fluorescent dye are fired simultaneously, and launch the fluorescence of different wave length；First detection unit and the second inspection
The fluorescence for surveying unit is two beams by being come out perpendicular to two cuvette surface launchings that are corresponding and being parallel to each other of excitation light path
The fluorescence composition of the opposite direction of the launch；
（b）The fluorescence for detecting fluorescence probe transmitting filters through the first collector lens in the first detection unit and the first fluorescence
Mirror, electric signal is converted into by the first photodiode；
（c）The fluorescence of reference internal standard fluorescent dye transmitting is through the second collector lens and the second fluorescence in the second detection unit
Optical filtering, electric signal is converted into by photodiode；
（d）The electric signal that first detection unit and the second detection unit obtain is amplified through data acquisition amplification system, then
Automatically controlled by data and handle to obtain result with network transmission system, as a result shown on the display device.
（e）The fluorescence intensity that the wave band of each tested substance detects is measured glimmering with reference internal standard fluorescent dye wave band
The ratio of luminous intensity, establish respective standard curve with corresponding tested substance concentration respectively.
To launch 350-800 nm light source, excitation source is selected from Single wavelength LED, multi-wavelength LED, swashed the excitation source
Light or mercury lamp；Described lamp condenser lens, selected from planoconvex lens, plano-concave mirror, meniscus lens, biconvex mirror, biconcave mirror, it is irregular thoroughly
Mirror；The photodiode is selected from general-purpose diode, multiplication diode or avalanche diode；The cuvette material is optics glass
Glass, quartz glass, PS, PE, PMMA or PP, cuvette are shaped as square column, cylinder or taper；The display device is matched somebody with somebody for instrument
Standby display, mobile phone, tablet personal computer or PC computers.
The data acquisition amplification system is used to amplify the electric signal after photodiode is changed；It is described automatically control with
The method of network transmission system processing data is：The ratio of light signal strength is detected with the photodiode of two detection units
Standard curve is established with tested substance concentration in cuvette；The electric supply system and excitation source, photodiode, number
It is electrically connected according to gathering amplification system, automatically controlling with network transmission system, display device；The light of excitation source transmitting passes through
Cuvette of the light source optical filter collector lens extremely equipped with fluorescence probe and reference internal standard fluorescent dye；Fluorescence probe in cuvette
Correspondence of the caused fluorescence through cuvette and two faces being parallel to each other are sent, sequentially passes through collector lens and optical filtering is injected
Photodiode, photodiode convert optical signals into electric signal, and electric signal amplifies through data acquisition amplification system, then passes through
Data automatically control to be handled to obtain result with network transmission system, is as a result shown on the display device.
Method concrete operations comprise the steps of：
（1）Light path is set：The fluorescence gone out from the correspondence of cuvette and two surface launchings being parallel to each other is divided into 2 light paths,
Corresponding reference internal standard fluorescent dye emitted luminescence intensity detection and corresponding another fluorescence probe emitted luminescence intensity detection；
（2）Establish the standard curve of tested substance：2 detection units detect the fluorescence of 2 tested substances respectively
The fluorescence intensity of intensity and 1 reference internal standard fluorescent dye, the fluorescence intensity detected by tested substance and reference dye
The ratio for the fluorescence intensity measured, respective standard curve is established with tested substance concentration；
（3）The Concentration Testing of tested substance：Tested substance and corresponding detection probe and reference fluorescent dyestuff are placed in
In cuvette, the light of excitation source transmitting passes through lamp condenser lens and optical filter to cuvette；Caused fluorescence in cuvette
2 faces through cuvette, through fluorescence collector lens and optical filtering, detection respectively obtains the fluorescence of tested substance and reference dye
Intensity；The ratio of fluorescence intensity is corresponding with the standard curve of corresponding tested substance again, obtains the dense of corresponding tested substance
Degree.
Single wavelength LED, multi-wavelength LED, laser, mercury lamp can be selected in excitation source, according to fluorescence probe excitation wavelength needs,
From the light source that can launch 350 ~ 800 nm.Preferentially from multi-wavelength LED as excitation source.
Launch the wavelength of light according to the excitation source of selection, the bandpass filter, narrow in the range of 300 ~ 800 nm can be selected
Band optical filter etc..
Light can be will transmit through from light lens requirement to be gathered in a bit, and planoconvex lens, plano-concave mirror, meniscus lens, biconvex can be selected
Mirror, biconcave mirror, irregular lens etc., preferentially from planoconvex lens.
The cuvette material is optical glass, quartz glass, PS（Polystyrene）、PMMA（Polymethyl methacrylate）
Or PP（Polypropylene）, shape may be designed as square column, cylinder, taper etc..
The wave-length coverage of fluorescence is passed through as requested, the bandpass filter in the range of 350-800 nm can be selected, arrowband is filtered
Mating plate etc..
Diode, multiplication diode, avalanche diode etc. can be selected according to fluorescence intensity power is received in photodiode.
Preferentially from general photodiode as photoelectric conversion device.
Photodiode and data acquisition amplification system link with data processing unit respectively, and data processing unit can be with
Dedicated display connects, and can also be connected by bluetooth, USB or wifi with displays such as mobile phone, ipad or PCs.
Beneficial effects of the present invention are：Instrument include optical system, electric supply system, amplifying device, automatically control with
Network transmission system, display device and detection unit；Optical system is by excitation source, lamp condenser lens, light source filter, ratio
Color ware, collector lens, optical filtering, photoelectric conversion device etc. form.The light of light source transmitting is through optical filtering and collector lens rate of induced polarization
Fluorescent probe molecule in color ware, the fluorescence that probe is sent are assembled through 2 lens in vertical 2 direction with exciting light, profit
Short-wave band fluorescence and long-wave band fluorescence are converted into electric signal in 2 photodiodes respectively with optical filtering.Electric signal is amplified,
Data processing, show tested substance concentration.Compared with the two waveband fluorescence intensity ratio type detecting system in single fluorescent emission face,
On the basis of the advantages of keeping detection stability and accuracy rate, dichroic mirror and fluorescence collector lens are not provided with the system structure,
Make fluorescence loss less, light path it is short, have higher sensitivity, lower detectable limit.Under the equal conditions of detection copper ion,
Double fluorescent emission faces compare with the optical system in single fluorescent emission face, fluoroscopic examination intensity improve 1.2 times, detectable limit by
0.03 mg/kg is reduced to 0.015 mg/kg.Therefore, the detector has higher sensitivity and lower detectable limit.This
The equipment instrument of invention is smaller, simple to operate, and the quick analysis available for live real-time quantitative detects.
Brief description of the drawings
The schematic diagram of the double fluorescent emission face fluorescence detectors of Fig. 1.
In figure：1st, excitation source, 2, lamp condenser lens, 2a, the first fluorescence collector lens, 2b, the second fluorescence optically focused are saturating
Mirror, 3, light source filter, 3a, the first fluorescence optical filtering, 3b, the second fluorescence optical filtering, 4, cuvette, 5a, the pole of the first photoelectricity two
Pipe, 5b, the second photodiode, 6, power supply, 7, data acquisition amplification system, 8, automatically control and network transmission system, 9, aobvious
Show equipment.
Embodiment
Embodiment 1：The fluorescence intensity of the two waveband detector in single fluorescent emission face and double single fluorescent emission faces
The two waveband detector experiment condition in single fluorescent emission face：Copper ion fluorescence probe（10 μM, Chinese invention patent
Compound 1 in ZL200410082745.3, the nm of maximum excitation wavelength 450, the fluorescence of the nm wavelength of probe emission 525, probe
Launch the fluorescence of 475 nm wavelength, LED excitation sources after being combined with copper ion（The nm of wavelength 450, the mW of rated power 40）, excite
The mW of power 40,50 mM HEPES buffered waters-ethanol solution（PH 7.4,30oC）, copper ion concentration（10 µM）, side
Shape standard colorimetric ware, various fluorescence collector lenses, dichroic mirror（500 nm）, A roads fluorescence optical filtering（475 nm, narrow-band-filter
Piece）, B roads fluorescence optical filtering（525 nm, bandpass filter）, collector lens（Planoconvex lens）, the M of multiplication factor 4.
The two waveband detector experiment condition in double fluorescent emission faces：Copper ion fluorescence probe（10 μM, Chinese invention patent
Compound 1 in ZL200410082745.3, the nm of maximum excitation wavelength 450, the fluorescence of the nm wavelength of probe emission maximum 525,
Probe is combined the rear nm of maximum emission wavelength 475 with copper ion）, LED excitation sources（The nm of wavelength 450, the mW of rated power 40）,
The mW of excitation power source power 40,50 mM HEPES buffered waters-ethanol solution（PH 7.4,30oC）, copper ion concentration（10 µ
M）, square standard colorimetric ware, the first fluorescence optical filtering（475 nm, narrow band pass filter）, the second fluorescence optical filtering（525 nm, band
Pass filter）, collector lens（Planoconvex lens）, the M of multiplication factor 4.
Experimental result：
The above results are shown, under the conditions of blank（Without copper ion）, the optical system in double fluorescent emission faces of the application patent
The B roads intensity of system detection copper fluorescence probe（Fluorescence intensity is 1197 mV）Than the result of single fluorescent emission face optical system（Fluorescence
Intensity is 550 mV）It is higher by 1.2 times.The result of copper ions situation is also similar.
Embodiment 2：The two waveband detector detection copper ion detectable limit in single or double fluorescent emission face
Experiment condition is the same as embodiment 1.
Experimental result：
The above results are shown, in the case of the optical system detection copper fluorescence probe in single fluorescent emission face, copper content by
When 0.03 mg/kg drops to 0.02 mg/kg, the fluorescence intensity on A and B roads only changes 1 mV, quick less than photodiode
Feel error range, therefore, this optical system detection limiting value is 0.03 mg/kg；With double fluorescent emission faces of the application patent
Optical system detection copper fluorescence probe in the case of, copper content by 0.015 mg/kg it is elevated to 0.020 mg/kg when, A and B
The mV of fluorescence intensity change 7 ~ 9 on road, also in the sensitive range of photodiode, therefore, this optical system detection limit
Value is less than 0.015 mg/kg.
Embodiment 3
The course of work of the detector is as shown in Figure 1：The light that excitation source 1 is launched is via collector lens 2 and optical filtering 3
It is irradiated to cuvette 4, the detection fluorescence probe and reference internal standard fluorescent dye in cuvette are fired simultaneously, and launch different ripples
Long fluorescence；The fluorescence of fluorescence probe transmitting is detected through the first fluorescence collector lens 2a and the first fluorescence in the first detection unit
Optical filtering 3a, electric signal is converted into by the first photodiode 5a；The fluorescence of reference internal standard fluorescent dye transmitting is through the second detection
The second fluorescence collector lens 2b and the second fluorescence optical filtering 3b in unit, telecommunications is converted into by the second fluorescence photodiode 5b
Number；Amplify in the electric signal that two detection units of A and B obtain through data acquisition amplification system 7, then by data automatically control with
The processing of network transmission system 8 obtains result, is as a result shown on display device 9.
Embodiment 4：Establish copper ion detection working curve
It is dense only to change copper ion with reference to the two waveband detector part in double fluorescent emission faces in embodiment 1 for experiment condition
Degree（0 ~ 10 µM）.
Experimental result：
Calculation formula：Y=k × A+b, A strength Calculation Results：K=- 4.1455, b=10.081 is related
Coefficients R2 = 0.9937；
Embodiment 5：Content of copper ion detects in running water
The two waveband detector part in double fluorescent emission faces in experiment condition reference embodiment 1,5 water samples are derived from difference
Place.The following results being calculated using k the and c values in example 4, compared with ICP-MS testing results, error 5% with
It is interior.
Claims (1)
1. a kind of minimal feeding instrument in double fluorescent emission faces, it includes power supply（6）, data acquisition amplification system（7）, it is automatic
Control and network transmission system（8）, display device（9）, it is characterised in that：It also include excite unit, the first detection unit and
Second detection unit；First detection unit is parallel with the second detection unit, excites unit to be mutually perpendicular to the first detection unit；Institute
State and excite unit to include excitation source（1）, lamp condenser lens（2）, light source filter（3）And cuvette（4）；First inspection
Surveying unit includes the first fluorescence collector lens（2a）, the first fluorescence optical filtering（3a）, the first photodiode（5a）；Described second
Detection unit includes the second fluorescence collector lens（2b）, the second fluorescence optical filtering（3b）, the second photodiode（5b）；Cuvette
（4）With at the first detection unit, the second detection unit on the same line；First photodiode（5a）Data are electrically connected to adopt
Collect amplification system（7）, automatically control and network transmission system（8）And display device（9）, the second photodiode（5b）Electrical connection
To data acquisition amplification system（7）, automatically control and network transmission system（8）And display device（9）.
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| CN201720484766.0U CN206684048U (en) | 2017-05-04 | 2017-05-04 | A kind of minimal feeding instrument in double fluorescent emission faces |
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| CN201720484766.0U CN206684048U (en) | 2017-05-04 | 2017-05-04 | A kind of minimal feeding instrument in double fluorescent emission faces |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970058A (en) * | 2017-05-04 | 2017-07-21 | 苏州尚稷电子科技有限公司 | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face |
| CN111693497A (en) * | 2020-05-15 | 2020-09-22 | 核工业北京化工冶金研究院 | Portable aquatic uranium detector |
-
2017
- 2017-05-04 CN CN201720484766.0U patent/CN206684048U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970058A (en) * | 2017-05-04 | 2017-07-21 | 苏州尚稷电子科技有限公司 | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face |
| CN111693497A (en) * | 2020-05-15 | 2020-09-22 | 核工业北京化工冶金研究院 | Portable aquatic uranium detector |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230627 Address after: 201102 room 801, No. 56, Lane 1266, Gudai Road, Minhang District, Shanghai Patentee after: Wang Zheng Address before: No. 6, Anmin Road, Huangdai Town, Xiangcheng District, Suzhou City, Jiangsu Province, 215131 Patentee before: SUZHOU SHANGJI ELECTRONIC TECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |