CN207662797U - The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe - Google Patents
The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe Download PDFInfo
- Publication number
- CN207662797U CN207662797U CN201721615227.2U CN201721615227U CN207662797U CN 207662797 U CN207662797 U CN 207662797U CN 201721615227 U CN201721615227 U CN 201721615227U CN 207662797 U CN207662797 U CN 207662797U
- Authority
- CN
- China
- Prior art keywords
- cuvette
- quantum dot
- detector
- fluorescence probe
- dual wavelength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
More particularly to a kind of fluorescent collecting device of dual wavelength quantum dot fluorescence probe of the utility model, including luminescence unit, first cuvette and the second cuvette, the luminescence unit projects the identical ultraviolet light of two-way to first, in two cuvettes, dual wavelength quantum dot fluorescence probe solution and solution to be measured are mixed in first cuvette, dual wavelength quantum dot fluorescence probe solution and water blanks are mixed in second cuvette, first, two cuvette sides are both provided with first, two detectors, first, two detectors are respectively used to receive excitation, the different fluorescence of wavelength simultaneously exports after being converted into electric signal；And the concentration detection system based on the fluorescent collecting device.The mixed liquor of solution to be measured and the mixed liquor of water blanks are placed respectively by the way that two cuvettes are arranged, and can be avoided error caused by the unstability of dual wavelength quantum dot fluorescence probe solution itself well, be improved the accuracy of testing result.
Description
Technical field
The utility model is related to heavy metal ion trace detection technical field, more particularly to a kind of dual wavelength quantum dot fluorescence
The fluorescent collecting device and concentration detection system of probe.
Background technology
A large amount of discharges of copper-bearing industrial wastewater cause water body, heavy metal copper in soil ion residues to sharply increase in recent years,
On the one hand excessive copper ion can cause the content of copper ion in crops to be accumulated, then growth retardation is so that yield declines；Separately
The intake of the excessive copper ion of one side can cause the bodies such as liver, the nerve of people damage that can not be converse.It is polluted in copper ion
With improvement, it is to pass to carry out quick, trace and accurate detection to copper ion residual concentration in water quality, soil and crop for monitoring
The first important link.Conventional copper ion detection method such as atomic absorption spectrum, electrochemistry, inductive coupling etc. needs Large expensive
Instrument, longer analytic process, complicated pre-treatment step and professional operation personage intervention, cannot meet the need of above-mentioned detection
It asks.Quantum dot fluorescence detection in recent years achieves preferable progress in the quick trace detection mechanism study of heavy metal ion, detects
Speed disclosure satisfy that detection demand with precision, but not form the detection device based on the principle, can not carry out in actual environment
Using detection.
Utility model content
The primary and foremost purpose of the utility model is to provide a kind of dual wavelength amount that can improve detection of heavy metal ion precision
The fluorescent collecting device of son point fluorescence probe.
In order to achieve the above object, the technical solution adopted in the utility model is：A kind of dual wavelength quantum dot fluorescence probe
Fluorescent collecting device, including luminescence unit, the first cuvette and the second cuvette, it is identical that the luminescence unit projects two-way
In ultraviolet light to the first and second cuvette, dual wavelength quantum dot fluorescence probe solution and solution to be measured are mixed in the first cuvette,
Dual wavelength quantum dot fluorescence probe solution and water blanks are mixed in second cuvette, the first and second cuvette side is all provided with
It is equipped with the first and second detector, the first and second detector is respectively used to fluorescence that receive excitation, that wavelength is different and is converted into telecommunications
It is exported after number.
Compared with prior art, there are following technique effects for the utility model：By the way that two cuvettes, two colorimetrics are arranged
The mixed liquor of the mixed liquor and water blanks of solution to be measured is placed in ware respectively, in this way, by the collection analysis to fluorescence, just
Error caused by the unstability of dual wavelength quantum dot fluorescence probe solution itself can be avoided well, improve testing result
Accuracy.
Another of the utility model is designed to provide a kind of concentration of high-precision dual wavelength quantum dot fluorescence probe
Detecting system.
In order to achieve the above object, the technical solution adopted in the utility model is：It is a kind of based on the dual wavelength quantum dot
The concentration detection system of the fluorescent collecting device of fluorescence probe, including fluorescent collecting device, Signal-regulated kinase, data processing mould
Block and power module, the first and second detector in fluorescent collecting device export electric signal to Signal-regulated kinase, signal condition
Module is amplified signal, filters, output is to data processing module after linearization process, and data processing module is to receiving
Signal carries out ratio proccessing and exports copper ion concentration after ratio is substituted into the interior standard curve stored, and standard curve is with to be measured molten
A concentration of abscissa of liquid, using ratio as ordinate；Power module is fluorescent collecting device, Signal-regulated kinase and data processing
Module for power supply.
Compared with prior art, there are following technique effects for the utility model：By the way that two cuvettes, two colorimetrics are arranged
The mixed liquor of the mixed liquor and water blanks of solution to be measured is placed in ware respectively, in this way, by the collection analysis to fluorescence, just
Error caused by the unstability of dual wavelength quantum dot fluorescence probe solution itself can be avoided well, improve testing result
Accuracy.
Description of the drawings
Fig. 1 is the structural schematic diagram of fluorescent collecting device embodiment one；
Fig. 2 is the vertical view of Fig. 1；
Fig. 3 is the structural schematic diagram of fluorescent collecting device embodiment two；
Fig. 4 is the functional block diagram of concentration detection system；
Fig. 5 is the circuit diagram of concentration detection system；
Fig. 6 is the schematic diagram of standard curve；
Fig. 7 is the work flow diagram of concentration detection system.
Specific implementation mode
With reference to Fig. 1 to Fig. 7, the utility model is described in further detail.
Refering to fig. 1, Fig. 3, a kind of fluorescent collecting device of dual wavelength quantum dot fluorescence probe, including luminescence unit 11,
One cuvette 12 and the second cuvette 13, the luminescence unit 11 project the identical ultraviolet light of two-way to the first and second cuvette
12, in 13, dual wavelength quantum dot fluorescence probe solution and solution to be measured, the second cuvette 13 are mixed in the first cuvette 12
In be mixed with dual wavelength quantum dot fluorescence probe solution and water blanks, the first and second cuvette 12,13 sides are both provided with
First and second detector 14,15, the first and second detector 14,15 are respectively used to fluorescence that receive excitation, that wavelength is different and conversion
At being exported after electric signal.By the way that two cuvettes are arranged, i.e. the first and second cuvette 12,13 is placed in two cuvettes and is waited for respectively
The mixed liquor of the mixed liquor and water blanks of solution is surveyed, in this way, by the collection analysis to fluorescence, can be avoided well double
Error caused by the unstability of wavelength quantum dot fluorescence probe solution itself, improves the accuracy of testing result.It needs
Bright is：Here " the first and second detector 14,15 " is writing a Chinese character in simplified form for " the first detector 14, the second detector 15 ", the same below
Reason.
In order to avoid the light between two cuvettes influences each other, in the utility model preferably, including shell 16, institute
The shell 16 stated includes two box chambers being disposed adjacent, and shell 16 is made of light-proof material, one of shell 16 side
On open up and be respectively communicated with two box chambers there are two through-hole, the first and second cuvette 12,13 is individually positioned in two box chambers of shell
In, the ultraviolet light that luminescence unit 11 projects distinguishes the first and second cuvette of directive 12,13 by two through-holes.By being arranged two
Adjacent box chamber facilitates and places the first and second cuvette 12,13, generally can be directly made of a box centre plus a partition board,
It can also be composed with two etuis.
In the utility model preferably, there are two for fixed setting on another side opposite with through-hole on the shell 16
Three detectors 17, the ultraviolet light that luminescence unit 11 projects expose to one of third after through-hole, the first cuvette 12 and visit
It surveys on device 17, the ultraviolet light that luminescence unit 11 projects exposes to another third detector after through-hole, the second cuvette 13
On 17, the corresponding peaks of third detector 17 are equal with the excitation wavelength of luminescence unit 11.After setting third detector 17,
The numerical value of two third detectors 17 is compared one by the stability that can detect the light source of the first and second cuvette of directive 12,13
The stability of light source can accurately just be quantified down.In this way, when light source stability is good, the data detected can be used as most
Terminate fruit；If when detection, it is found that light source stability is bad, then can detect again, to improve the accuracy of detection.
In order to facilitate each detector is arranged, in the utility model preferably, 12 side of the first cuvette setting
The vertical line between through-hole, third detector 17 of line between first and second detector 14,15, specific structure is from Fig. 2
In can be clearly seen that：First cuvette 12 is located at centre, and third detector, through-hole are located at the first cuvette 12
Front and back position, the first and second detector 14,15 are located at the left and right position of the first cuvette.The first and second of second cuvette, 13 side
Detector 14,15 is arranged in the same way.In this way, can ensure processing data to avoid the interference between each detector
Accuracy, meanwhile, it is fixedly mounted also very convenient.
There are many kinds of the structures of luminescence unit 11, provides two kinds of preferred embodiments here for reference.
Embodiment one, as shown in Figure 1 and Figure 2, the luminescence unit 11 include laser 111,112 and of half-reflecting half mirror
The light half that laser 111 emits is reflexed to the first cuvette 12 by total reflective mirror 113, half-reflecting half mirror 112, the other half transmission
After half-reflecting half mirror 112 the second cuvette 13 is reflexed to by total reflective mirror 113.The light path is simple, reliable, and the phase same sex of light is non-
Chang Hao.
Embodiment two, as shown in figure 3, the luminescence unit 11 includes strip ultraviolet lamp 114, strip ultraviolet lamp 114 is flat
Row distinguishes the first and second colorimetric of directive in that side for offering through-hole on shell 16, the light that strip ultraviolet lamp 114 projects
Ware 12,13.In the present embodiment, need the strip ultraviolet lamp 114 for selecting paralleling effect good just can be with light path is seldom, user
Just.
Refering to Fig. 4, Fig. 5, additionally provided in the utility model a kind of based on the glimmering of the dual wavelength quantum dot fluorescence probe
The concentration detection system of optical acquisition device, including fluorescent collecting device 10, Signal-regulated kinase 20, data processing module 30 and
Power module 40, the first and second detector 14,15 in fluorescent collecting device 10 export electric signal to Signal-regulated kinase 20, letter
Number conditioning module 20 is amplified signal, filters, after linearization process output to data processing module 30, data processing module
30 pairs of signals received carry out ratio proccessing and export copper ion concentration, standard after ratio is substituted into the interior standard curve stored
Curve using solution concentration to be measured as abscissa, using ratio as ordinate, it is shown in fig. 6 be exactly using copper ion concentration as abscissa,
The standard curve drawn as ordinate using ratio K；Power module 40 is fluorescent collecting device 10, Signal-regulated kinase 20 and number
It powers according to processing module 30.This system can not only be used for detecting copper ion concentration, can also be used to detect other heavy metals
Ion concentration, it is only necessary to compatible dual wavelength quantum dot fluorescence probe solution and first and second are selected according to solution to be measured
Detector 14,15, and different standard curves is stored in data processing module 30.
Patent of invention filed in 2 days June in 2017 of applicant《Dual wavelength quantum dot fluorescence probe furans phenolic ketone detection dress
It sets and its detection method》In disclose it is a kind of such as preceding dual wavelength quantum dot fluorescence probe furans phenolic ketone detection method including as follows
Step：(A) start luminescence unit, read detection initial value m11, m21 of the first and second photodetector；It is added into cuvette mixed
The dual wavelength quantum dot fluorescence probe solution and sample solution being combined read again the first and second light after waiting for reaction 2 minutes
Detected value m12, m22 of electric explorer；Ratio k=(m22/m12)/(m21/m11)；(B) by multiple known furans phenolic ketone concentration
Solution executes step A as sample solution and obtains multiple ratios, is drawn as ordinate using a concentration of abscissa of furans phenolic ketone, ratio
Standard curve；(C) solution to be measured is executed into step A as sample solution and obtains ratio K, ratio K is corresponding on standard curve
Abscissa is exactly the furans phenolic ketone concentration of solution to be measured.Improvement obtains on the step of scheme in the application is above：It is right
First and second cuvette 12,13 obtains ratio k 1 and k2 by processing of step A respectively, take k1/k2 as K values come execute step B and
C, specific flow are as shown in Figure 7.
Furans phenolic ketone concentration if to be detected, the peak value of response of the first and second detector 14,15 be respectively 417nm,
550nm, 17 peak value of response of third detector are 355nm；If to be detected is copper ion concentration, the first and second detector
14,15 corresponding peaks are respectively 550nm, 650nm, and the peak value of response of third detector 17 is 265nm.If it is other from
Sub- concentration can select corresponding corresponding peaks, no longer illustrate one by one here.
Preferably, it is fixedly installed on another side opposite with through-hole on the shell 16 there are two third detector 17,
The ultraviolet light that luminescence unit 11 projects exposes to after through-hole, the first cuvette 12 on one of third detector 17, sends out
The ultraviolet light that light unit 11 projects exposes to after through-hole, the second cuvette 13 on another third detector 17, and third is visited
The corresponding peaks for surveying device 17 are equal with the excitation wavelength of luminescence unit 11；The electric signal that third detector 17 exports is by letter
Number conditioning module 20 is amplified, filters, output is to data processing module 30 after linearization process, and data processing module 30 is by two
The data that a third detector 17 exports are compared to judge the stability of light source.Third detector 17 is set, it can be fine
Ground is detected the stability of light source, ensures that system is operated in the state of light stability.
Further, including USB connector 50 and portable terminal 60, the copper ion concentration that data processing module 30 exports
By the output of USB connector 50 on portable terminal 60, portable terminal 60 be used to show, store, upload, check the copper of detection from
Sub- concentration.By the way that portable terminal 60 is arranged, the personnel that can be conveniently operated handle data.
Claims (9)
1. a kind of fluorescent collecting device of dual wavelength quantum dot fluorescence probe, it is characterised in that：Including luminescence unit (11), first
Cuvette (12) and the second cuvette (13), the luminescence unit (11) project the identical ultraviolet light of two-way to the first and second ratio
In color ware (12,13), dual wavelength quantum dot fluorescence probe solution and solution to be measured, the second ratio are mixed in the first cuvette (12)
Dual wavelength quantum dot fluorescence probe solution and water blanks, the first and second cuvette (12,13) side are mixed in color ware (13)
Be both provided with the first and second detector (14,15), the first and second detector (14,15) be respectively used to receive excitation, wavelength it is different
Fluorescence and export after being converted into electric signal.
2. the fluorescent collecting device of dual wavelength quantum dot fluorescence probe as described in claim 1, it is characterised in that：Including shell
(16), the shell (16) includes two box chambers being disposed adjacent, and shell (16) is made of light-proof material, shell (16)
Through-hole is respectively communicated with two box chambers there are two being opened up on one of side, and the first and second cuvette (12,13) is individually positioned in outer
In two box chambers of shell, ultraviolet light that luminescence unit (11) projects by two through-holes distinguish the first and second cuvettes of directive (12,
13)。
3. the fluorescent collecting device of dual wavelength quantum dot fluorescence probe as claimed in claim 2, it is characterised in that：The shell
(16) there are two the purples that third detector (17), luminescence unit (11) project for fixed setting on the another side opposite with through-hole on
Outer light is exposed to after through-hole, the first cuvette (12) on one of third detector (17), and luminescence unit (11) projects
Ultraviolet light exposed to after through-hole, the second cuvette (13) on another third detector (17), third detector (17)
Corresponding peaks it is equal with the excitation wavelength of luminescence unit (11).
4. the fluorescent collecting device of dual wavelength quantum dot fluorescence probe as claimed in claim 3, it is characterised in that：Described
One cuvette (12) side setting the first and second detector (14,15) between line vertically with through-hole, third detector (17)
Between line；First and second detector (14,15) of the second cuvette (13) side is arranged in the same way.
5. the fluorescent collecting device of dual wavelength quantum dot fluorescence probe as claimed in claim 3, it is characterised in that：The hair
Light unit (11) includes laser (111), half-reflecting half mirror (112) and total reflective mirror (113), and half-reflecting half mirror (112) is by laser
The light half of device (111) transmitting reflexes to the first cuvette (12), the other half through after half-reflecting half mirror (112) by being all-trans
Mirror (113) reflexes to the second cuvette (13).
6. the fluorescent collecting device of dual wavelength quantum dot fluorescence probe as claimed in claim 3, it is characterised in that：The hair
Light unit (11) includes strip ultraviolet lamp (114), and strip ultraviolet lamp (114) is parallel to that through-hole is offered on shell (16)
Side, light difference the first and second cuvette of directive (12,13) that strip ultraviolet lamp (114) projects.
7. a kind of dual wavelength quantum dot fluorescence probe concentration detection system, it is characterised in that：Including fluorescent collecting device (10), letter
Number conditioning module (20), data processing module (30) and power module (40)；Fluorescent collecting device (10) includes luminescence unit
(11), the first cuvette (12) and the second cuvette (13), the luminescence unit (11) project the identical ultraviolet light of two-way extremely
In first and second cuvette (12,13), dual wavelength quantum dot fluorescence probe solution and to be measured molten is mixed in the first cuvette (12)
Liquid is mixed with dual wavelength quantum dot fluorescence probe solution and water blanks, the first and second cuvette in the second cuvette (13)
(12,13) side is both provided with the first and second detector (14,15), and the first and second detector (14,15) is respectively used to receive excitation
, the fluorescence that wavelength is different and export after being converted into electric signal；In fluorescent collecting device (10) the first and second detector (14,
15) to Signal-regulated kinase (20), Signal-regulated kinase (20) is amplified signal, filters, at linearisation output electric signal
Output is to data processing module (30) after reason, and data processing module (30) carries out ratio proccessing to the signal received and by ratio
Copper ion concentration is exported after the standard curve stored in substituting into, standard curve is as abscissa, with ratio using solution concentration to be measured
Ordinate；Power module (40) is that fluorescent collecting device (10), Signal-regulated kinase (20) and data processing module (30) supply
Electricity.
8. dual wavelength quantum dot fluorescence probe concentration detection system as claimed in claim 7, it is characterised in that：Including shell
(16), the shell (16) includes two box chambers being disposed adjacent, and shell (16) is made of light-proof material, shell (16)
Through-hole is respectively communicated with two box chambers there are two being opened up on one of side, and the first and second cuvette (12,13) is individually positioned in outer
In two box chambers of shell, ultraviolet light that luminescence unit (11) projects by two through-holes distinguish the first and second cuvettes of directive (12,
13)；There are two third detector (17), luminescence units for fixed setting on the another side opposite with through-hole on the shell (16)
(11) ultraviolet light projected is exposed to after through-hole, the first cuvette (12) on one of third detector (17), is shone
The ultraviolet light that unit (11) projects is exposed to after through-hole, the second cuvette (13) on another third detector (17), the
The corresponding peaks of three detectors (17) are equal with the excitation wavelength of luminescence unit (11)；The telecommunications of third detector (17) output
Number be amplified, filter by Signal-regulated kinase (20), output is to data processing module (30) after linearization process, at data
The data that two third detectors (17) export are compared to judge the stability of light source by reason module (30).
9. dual wavelength quantum dot fluorescence probe concentration detection system as claimed in claim 8, it is characterised in that：Connect including USB
Device (50) and portable terminal (60) are connect, the copper ion concentration of data processing module (30) output is exported by USB connector (50)
To portable terminal (60), portable terminal (60) is used to show, store, upload, check the copper ion concentration of detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721615227.2U CN207662797U (en) | 2017-11-28 | 2017-11-28 | The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721615227.2U CN207662797U (en) | 2017-11-28 | 2017-11-28 | The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207662797U true CN207662797U (en) | 2018-07-27 |
Family
ID=62946481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721615227.2U Active CN207662797U (en) | 2017-11-28 | 2017-11-28 | The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207662797U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655437A (en) * | 2018-12-18 | 2019-04-19 | 上海交通大学 | A kind of two-parameter Dynamic Fluorescence detection system and method |
| CN115436301A (en) * | 2022-09-05 | 2022-12-06 | 成都信息工程大学 | BP neural network-based mixed ion concentration sensing system |
-
2017
- 2017-11-28 CN CN201721615227.2U patent/CN207662797U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655437A (en) * | 2018-12-18 | 2019-04-19 | 上海交通大学 | A kind of two-parameter Dynamic Fluorescence detection system and method |
| CN115436301A (en) * | 2022-09-05 | 2022-12-06 | 成都信息工程大学 | BP neural network-based mixed ion concentration sensing system |
| CN115436301B (en) * | 2022-09-05 | 2023-06-09 | 成都信息工程大学 | BP neural network-based mixed ion concentration sensing system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104198391B (en) | Ultraviolet fluorescence double-signal water quality monitoring device taking LED (light emitting diode) as light source and application method of device | |
| CN100520365C (en) | Method and device for classified detecting density of phytoplankton under water in site | |
| CN102661923A (en) | Complex monitor for automatically monitoring multiple parameters of water on line | |
| CN207662797U (en) | The fluorescent collecting device and concentration detection system of dual wavelength quantum dot fluorescence probe | |
| CN103884706B (en) | Online original pulp liquor detection and classification system | |
| CN203432903U (en) | Double-wavelength underwater fluorescence detection device | |
| CN102928390A (en) | On-line detection method and device for chlorophyll concentration in water body based on two detectors | |
| CN206546331U (en) | The fluorescence immunity analyzer of illegal drug in a kind of quick detection food | |
| CN101464409B (en) | Apparatus and method for fast quantitative bacteria detection | |
| CN102590123A (en) | Method for detecting content of bismuth in bismuth potassium citrate medicament | |
| CN206420773U (en) | A kind of portable formaldehyde examination device based on fluorescence probe method | |
| CN107389644A (en) | A kind of rapid fluorescence proportioning device | |
| CN106083645A (en) | A kind of iron-ion fluorescent probe compound and its preparation method and application | |
| CN103134768A (en) | Portable special gutter oil detection system based on near infrared spectral technology | |
| CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
| CN210037588U (en) | Absorption spectrum test system | |
| CN206684048U (en) | A kind of minimal feeding instrument in double fluorescent emission faces | |
| CN203164119U (en) | Two-photon absorption section spectrometer | |
| Chen et al. | A practical portable photometer using LEDs as inspection light source | |
| CN111024644B (en) | Single-wavelength-based rapid optical detection method and device for pH value of water | |
| CN112147101A (en) | Portable water quality analyzer and method for soluble organic matters and nitrate nitrogen | |
| CN2909242Y (en) | Instrument for quickly investigating component content of fertilizer | |
| CN210604388U (en) | Device for rapidly and accurately detecting wheat grain vomitoxin through differential single-photon Raman sensing | |
| CN112285047A (en) | Chemical oxygen demand and nitrate nitrogen water quality monitoring probe and method | |
| CN204649626U (en) | A kind of portable detection equipment based on FRET principle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant |