CN203432903U - Double-wavelength underwater fluorescence detection device - Google Patents
Double-wavelength underwater fluorescence detection device Download PDFInfo
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- CN203432903U CN203432903U CN201320598997.6U CN201320598997U CN203432903U CN 203432903 U CN203432903 U CN 203432903U CN 201320598997 U CN201320598997 U CN 201320598997U CN 203432903 U CN203432903 U CN 203432903U
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- detection device
- double
- wavelength
- fluorescence detection
- emitting diode
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 19
- 230000005284 excitation Effects 0.000 claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 23
- 230000003287 optical effect Effects 0.000 claims abstract description 20
- 230000009977 dual effect Effects 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000835 fiber Substances 0.000 claims description 10
- 239000002775 capsule Substances 0.000 claims description 9
- 230000008878 coupling Effects 0.000 claims description 5
- 238000010168 coupling process Methods 0.000 claims description 5
- 238000005859 coupling reaction Methods 0.000 claims description 5
- 239000013307 optical fiber Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000003595 spectral effect Effects 0.000 abstract 1
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
Images
Abstract
The utility model provides a double-wavelength underwater fluorescence detection device. The double-wavelength underwater fluorescence detection device comprises a sealed cabin shell, wherein an optical window is arranged at the front end of the sealed cabin shell and a water-tight electric plug is arranged at the rear end of the sealed cabin shell. The double-wavelength underwater fluorescence detection device is characterized in that the sealed cabin shell is internally provided with a double-wavelength excitation light source controlled by an electronic control power supplying module and a spectrograph connected with the electronic control power supplying module; a collection light path is arranged between the optical window and the spectrograph; the collection light path is internally provided with a trapped wave optical filter; the trapped wave optical filter is used for simultaneously filtering scattered light which has two wavelengths and is emitted by the double-wavelength excitation light source. The double-wavelength excitation light source comprises a 365nm light emitting diode and a 530nm light emitting diode. The underwater fluorescence detection device provided by the utility model adopts one single spectrograph to simultaneously meet light splitting detection of double-wavelength excitation fluorescence and obtain the high-resolution spectral information; meanwhile, the cost is saved, the development difficulty of instruments is reduced and the double-wavelength underwater fluorescence detection device has important practical value.
Description
Technical field
The utility model relates to a kind of spectrographic detection sensor, and a kind of dual wavelength fluorescence detection device under water specifically, for the detection of organic component under water.
Technical background
Photofluorometer is the instrument the most often using in organic component fluorescence detection under water, its principle is to adopt light emitting diode transmitting exciting light, utilize photodiode to receive emitting fluorescence signal, adopt photofluorometer to survey organic component compact volume and convenience under water, measure fast, be applicable to detecting in real time under water, but mostly photofluorometer measurement result is in the market the intensity level of single fluorescence spectrum peak, quantity of information is not enough., more comprehensive information more for obtaining, adopting many excitation wavelengths and high-resolution detection is the trend of fluorescence detection device under water.
Summary of the invention
The purpose of this utility model is to provide a kind of dual wavelength fluorescence detection device under water, can measure the main organic component such as yellow substance, chlorophyll in water body, to overcome the deficiencies in the prior art.
A kind of dual wavelength is fluorescence detection device under water, comprise that front end is provided with the pressurized capsule housing that optical window, rear end are provided with watertight plug, it is characterized in that being provided with in pressurized capsule housing the dual wavelength excitation source of controlling by Electronic Control supply module, and the spectrometer being connected with Electronic Control supply module, also have the collection light path between optical window and spectrometer; Described collection light path includes notch filtering light sheet, the scattered light of two kinds of wavelength that this notch filtering light sheet is launched for the dual wavelength of filtering simultaneously excitation source.
Described dual wavelength excitation source comprises 365nm light emitting diode and 530nm light emitting diode.
The excitation light path matching with described 365nm light emitting diode comprises 365nm bandpass filter and the condenser lens that is successively set on 365nm light emitting diode the place ahead; The excitation light path matching with described 530nm light emitting diode comprises 530nm bandpass filter and the condenser lens that is successively set on 530nm light emitting diode the place ahead.
Described collection optical routing successively notch filtering light sheet, the coupling fiber lens of signal collection lens, 530nm or the 532nm between optical window and spectrometer and the optical fiber being connected with spectrometer forms.
For the device of fluorescence detection under water of employing fiber spectrometer, generally each excitation wavelength adopts a fiber spectrometer to divide photodetection, and before each fiber spectrometer, places the long pass filter filtering exciting light of specific wavelength.The utility model adopts the LED of two wavelength as excitation source, but has only adopted a spectrometer to divide photodetection, and its core technology has been to utilize commercially available notch filtering light sheet.The business-like 532nm notch filtering light sheet of take is example, this optical filter has very high rejection ratio (OD>6) for 532nm center 10nm bandwidth, but at the wavelength place that is less than 375nm, also there is very high rejection ratio simultaneously, its transmittance curve as shown in Figure 1, therefore can utilize a tablet filter to realize the inhibition of two kinds of exciting lights, the utility model adopts 530nm and 365nmLED respectively chlorophyll and yellow substance to be excited as excitation source, and business-like 532nm notch filtering light sheet is the exciting light of filtering 365nm and 530nmLED simultaneously.
The utility model under water fluorescence detection device adopts an independent fiber spectrometer to meet minute photodetection of dual wavelength fluorescence excitation simultaneously, both can obtain high-resolution spectroscopy information, saves again cost simultaneously, and lowering apparatus development difficulty, has important practical value.
Accompanying drawing explanation
The 532nm notch filtering light sheet transmittance curve that Fig. 1 the utility model adopts.
Fig. 2 general structure schematic diagram of the present utility model.
Wherein, 1 is pressurized capsule housing, and 2 is 365nm light emitting diode, 3 is 365nm bandpass filter, and 4 is condenser lens, and 5 is optical window, 6 is 530nm light emitting diode, and 7 is 530nm bandpass filter, and 8 is condenser lens, 9 is signal collection lens, and 10 is 530nm notch filtering light sheet, and 11 is coupling fiber lens, 12 is optical fiber, 13 is spectrometer, and 14 is Electronic Control/supply module, and 15 is watertight plug.
Embodiment
As shown in Figure 2, a kind of dual wavelength is fluorescence detection device under water, comprise that front end is provided with the pressurized capsule housing 1 that optical window 5, rear end are provided with watertight plug 15, it is characterized in that being provided with by the dual wavelength excitation source of Electronic Control supply module 14 controls in pressurized capsule housing 1, and the spectrometer 13 being connected with Electronic Control supply module 14, also have the collection light path between optical window 5 and spectrometer 13; Described collection light path includes notch filtering light sheet 10, the scattered light of two kinds of wavelength that this notch filtering light sheet 10 is launched for while filtering dual wavelength excitation source.
Embodiment
As shown in Figure 2, the utility model is by the dual wavelength excitation source in the optical window 5 on pressurized capsule housing 1, housing and watertight plug 15, housing, the dual wavelength excitation source of the present embodiment is the excitation source that two covers are comprised of light emitting diode and condenser lens: 365nm light emitting diode 2 and 530nm light emitting diode 6, and its excitation light path is respectively: 365nm bandpass filter 3, condenser lens 4; With 530nm bandpass filter 7 and condenser lens 8.
Collection light path in housing: signal collection lens 9,530nm or 532nm notch filtering light sheet 10, coupling fiber lens 11 and optical fiber 12; Also have spectrometer 13 and Electronic Control supply module more than 14 parts, by 10 of existing notch filtering lights, can realize minute photodetection that adopts an independent fiber spectrometer 13 simultaneously to meet dual wavelength fluorescence excitation.
During work, the 365nm light emitting diode 2 and the 530nm light emitting diode 6 that are contained in pressurized capsule housing 1 are simultaneously luminous, 365nm exciting light is through bandpass filter 3, condenser lens 4 and optical window 5 focus on measured matter out of my cabin, 530nm exciting light is through bandpass filter 7, condenser lens 8 and optical window 5 focus on same position out of my cabin, the fluorescence signal that measured matter sends is collected by lens 9 by optical window 5, collimation, then through 10 while of 530nm notch filtering light sheet filtering 365nm and 530nm Rayleigh scattering light, through coupling fiber lens 11, fluorescence signal is coupled to optical fiber 12 again, enter afterwards spectrometer 13 and carry out light splitting, survey, finally data are deposited in to Electronic Control/supply module 14.
Claims (4)
1. dual wavelength fluorescence detection device under water, comprise that front end is provided with the pressurized capsule housing (1) that optical window (5), rear end are provided with watertight plug (15), it is characterized in that being provided with by the dual wavelength excitation source of Electronic Control supply module (14) control in pressurized capsule housing (1), and the spectrometer (13) being connected with Electronic Control supply module (14), be positioned in addition the collection light path between optical window (5) and spectrometer (13); Described collection light path includes notch filtering light sheet (10), the scattered light of two kinds of wavelength that this notch filtering light sheet (10) is launched for while filtering dual wavelength excitation source.
2. dual wavelength as claimed in claim 1 fluorescence detection device under water, is characterized in that described dual wavelength excitation source comprises 365nm light emitting diode (2) and 530nm light emitting diode (6).
3. dual wavelength as claimed in claim 2 fluorescence detection device under water, is characterized in that the excitation light path matching with described 365nm light emitting diode (2) comprises the 365nm bandpass filter (3) and condenser lens (4) that is successively set on 365nm light emitting diode (2) the place ahead; The excitation light path matching with described 530nm light emitting diode (6) comprises 530nm bandpass filter (7) and the condenser lens (8) that is successively set on 530nm light emitting diode (6) the place ahead.
4. dual wavelength as claimed in claim 2 fluorescence detection device under water, is characterized in that described collection optical routing is positioned at notch filtering light sheet (10), the coupling fiber lens (11) of signal collection lens (9), 530nm or 532nm between optical window (5) and spectrometer (13) and optical fiber (1) composition being connected with spectrometer (13) successively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320598997.6U CN203432903U (en) | 2013-09-26 | 2013-09-26 | Double-wavelength underwater fluorescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320598997.6U CN203432903U (en) | 2013-09-26 | 2013-09-26 | Double-wavelength underwater fluorescence detection device |
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| Publication Number | Publication Date |
|---|---|
| CN203432903U true CN203432903U (en) | 2014-02-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320598997.6U Expired - Fee Related CN203432903U (en) | 2013-09-26 | 2013-09-26 | Double-wavelength underwater fluorescence detection device |
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| Country | Link |
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| CN (1) | CN203432903U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906132A (en) * | 2017-03-21 | 2017-06-30 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Digital polymerase chain reaction optical detection apparatus and method |
| CN108088835A (en) * | 2018-02-06 | 2018-05-29 | 清华大学深圳研究生院 | A kind of underwater detecting system based on laser induced breakdown spectroscopy |
| CN109959452A (en) * | 2019-04-02 | 2019-07-02 | 浙江大学 | A kind of underwater fluorescence spectrum imager of pull-type and imaging method |
| CN110487760A (en) * | 2019-08-23 | 2019-11-22 | 山东省科学院海洋仪器仪表研究所 | Optical module that is a kind of while measuring light oil and mink cell focus in water |
| CN112881349A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Optical probe applied to underwater fluorescence detection |
| CN112881322A (en) * | 2021-01-18 | 2021-06-01 | 清华大学 | Immersed ultraviolet-visible absorption spectrum sensor and using method thereof |
| CN112881348A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Underwater fluorescence sensor |
-
2013
- 2013-09-26 CN CN201320598997.6U patent/CN203432903U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906132A (en) * | 2017-03-21 | 2017-06-30 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Digital polymerase chain reaction optical detection apparatus and method |
| CN106906132B (en) * | 2017-03-21 | 2018-06-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Digital polymerase chain reaction optical detection apparatus and method |
| CN108088835A (en) * | 2018-02-06 | 2018-05-29 | 清华大学深圳研究生院 | A kind of underwater detecting system based on laser induced breakdown spectroscopy |
| CN109959452A (en) * | 2019-04-02 | 2019-07-02 | 浙江大学 | A kind of underwater fluorescence spectrum imager of pull-type and imaging method |
| CN110487760A (en) * | 2019-08-23 | 2019-11-22 | 山东省科学院海洋仪器仪表研究所 | Optical module that is a kind of while measuring light oil and mink cell focus in water |
| CN112881349A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Optical probe applied to underwater fluorescence detection |
| CN112881348A (en) * | 2019-11-29 | 2021-06-01 | 中国科学院大连化学物理研究所 | Underwater fluorescence sensor |
| CN112881322A (en) * | 2021-01-18 | 2021-06-01 | 清华大学 | Immersed ultraviolet-visible absorption spectrum sensor and using method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140212 Termination date: 20140926 |
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| EXPY | Termination of patent right or utility model |