CN212111141U - Portable optical detector - Google Patents
Portable optical detector Download PDFInfo
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- CN212111141U CN212111141U CN202020751116.XU CN202020751116U CN212111141U CN 212111141 U CN212111141 U CN 212111141U CN 202020751116 U CN202020751116 U CN 202020751116U CN 212111141 U CN212111141 U CN 212111141U
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- filter
- detection system
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- optical detection
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- 230000003287 optical effect Effects 0.000 title claims abstract description 117
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- 230000007717 exclusion Effects 0.000 claims abstract description 20
- 238000012360 testing method Methods 0.000 claims abstract description 12
- 230000005284 excitation Effects 0.000 claims description 15
- 238000004458 analytical method Methods 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 claims 9
- 230000035945 sensitivity Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
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- 238000002189 fluorescence spectrum Methods 0.000 description 2
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- 238000003745 diagnosis Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a portable optical detection instrument belongs to optical detection technical field. Light emitted by a light source (10) is received by an optical detector (50) after passing through a preposed optical detection system, a sample test tube placing part (20) and a postposition optical detection system, and the preposed optical detection system sequentially comprises a lens I (12), an optical filter I (14), a mutual exclusion optical filter I (15) and a diaphragm I (16); the rear optical detection system is sequentially provided with a spherical lens (40), a diaphragm II (41), a mutual exclusion filter II (43), an optical filter II (42) and a lens III (44), wherein the spherical lens (40) collects fluorescence within an angle of nearly 90 degrees, and the fluorescence passes through the diaphragm II (41) and the mutual exclusion filter II (43) and then reaches the optical detector (50) through the optical filter II (42) and the lens III (44). The utility model discloses portable optical detection instrument, small, the low and detection precision height of noise.
Description
Technical Field
The utility model relates to a portable optical detection instrument belongs to optical detection technical field for food sanitation, medical treatment and biological science field detect weak fluorescence signal.
Background
At present, fluorescence detection is widely applied to the industries of biology, chemistry, medicine, food safety and the like, and is an essential detection means in biological DNA detection and sequencing, chemical molecule and material component identification, and medical cancer cell diagnosis; such detection is achieved by large-scale fluorescence spectrometers. The spectrometer has the advantages of high sensitivity, wide spectral range and the disadvantages of high cost and large required sample amount, and is not suitable for on-site real-time detection.
Disclosure of Invention
An object of the utility model is to overcome above-mentioned not enough, provide a small portable optical detection instrument.
The purpose of the utility model is realized like this:
the utility model provides a portable optical detector, which comprises a preposed optical detection system, a sample test tube placing part, a postpositive optical detection system and an analysis processing system,
a light source is arranged in front of the front optical detection system, an optical detector for receiving a fluorescence signal detected by the optical detection system is arranged behind the rear optical detection system, light emitted by the light source is received by the optical detector after passing through the front optical detection system, a sample test tube placing position and the rear optical detection system, the front optical detection system sequentially comprises a lens I, an optical filter I, a mutual exclusion optical filter I and a diaphragm I, and the light source is arranged in front of the lens I;
the rear optical detection system is sequentially provided with a spherical lens, a diaphragm II, a mutual exclusion filter II, an optical filter II and a lens III, and the lens III is arranged in front of the optical detector; the spherical lens collects the fluorescence within an angle of nearly 90 degrees, passes through a diaphragm II, a mutual exclusion filter II, an optical filter II and a lens III to reach an optical detector; the spherical lens and the lens I form a 90-degree optical confocal system;
the analysis processing system comprises a CPU control circuit, a primary amplifier, a secondary amplifier, a digital-to-analog conversion circuit, a touch screen control circuit, a digital display circuit and a USB interface circuit, wherein the optical detector is connected with the CPU control circuit through the primary amplifier, the CPU control circuit is connected with the light source sequentially through the digital-to-analog conversion circuit and the secondary amplifier, and the touch screen control circuit, the digital display circuit and the USB interface circuit are respectively connected with the CPU control circuit.
Optionally, the filter i is a low-pass or band-pass filter.
Optionally, the filter ii is a dielectric film or an absorptive filter.
Optionally, the filter ii is a long-pass filter or a band-pass filter.
Optionally, the optical detector includes, but is not limited to, a silicon photodetector, an integrated photodetector, an avalanche photodiode detector, or a photo-voltaic amplifier.
Optionally, the light source is an LED as an excitation light source.
Optionally, the mutually exclusive filter II is a pair of crossed linear polarizers.
Optionally, the mutually exclusive filter i is a pair of crossed linear polarizers.
Advantageous effects
The utility model provides a portable optical detector, because spherical lens and lens I form a 90 degrees optics confocal system, spherical lens can receive more signals and weaker signal, thus make the fluorescence that the sample sent can be collected by the maximize, make the whole structure smaller and more exquisite simultaneously, conveniently carry; the sample and the detector are respectively in a symmetrical structure relative to the spherical lens and the lens III and are positioned on the focus of the lens, the structure can ensure that fluorescence emitted by the sample can parallelly penetrate through the optical filter II, the crossed linear polarizer is used for further carrying out optical isolation, the sensitivity of the compact fluorometer is improved, the defect of light transmission wavelength displacement of the interference optical filter caused by different incidence angles is overcome, and the detection precision is improved.
Drawings
Fig. 1 is a schematic structural diagram of the portable optical detector of the present invention;
FIG. 2 is a graph of the sensitivity validation test results using a 365 nm excitation wavelength;
FIG. 3 is a graph of signal linearity verification test results using a 365 nm excitation wavelength;
FIG. 4 is a schematic structural diagram of another embodiment of FIG. 1;
in the figure:
Lens I12
Optical filter I14
Mutual exclusion filter I15
Diaphragm I16
Diaphragm II 41
Optical filter II 42
Mutual exclusion filter II 43
Lens III 44
An optical detector 50.
Detailed Description
Referring to fig. 1 and 2, the present invention relates to a portable optical detector, which includes a front optical detection system, a sample tube placing part 20, a rear optical detection system and an analysis processing system.
A light source 10 is arranged in front of the front optical detection system, and the light source 10 uses an LED as an excitation light source. An optical detector 50 is arranged behind the rear optical detection system and receives the fluorescent signal detected by the optical detection system. The light emitted from the light source 10 is received by the optical detector 50 after passing through the front optical detection system, the sample tube placing part 20 and the rear optical detection system. The optical detector 50 includes, but is not limited to, a silicon photodetector, an integrated photodetector, an avalanche photodiode detector, or a photo-voltaic amplifier.
The preposed optical detection system sequentially comprises a lens I12, an optical filter I14, a mutual exclusion optical filter I15 and a diaphragm I16, wherein the lens I12 is arranged in front of a light source 10, and the optical filter I14 is an excitation optical filter (low-pass or band-pass) and is used for filtering out the part of excitation light which is overlapped with the fluorescence spectrum component of a sample and only penetrates through fluorescence which can be effective to the sample. The diaphragm I16 is used for restraining exciting light in space and preventing the exciting light from irradiating a larger range of a test tube wall, so that scattered light is generated to influence a measurement result. The filter I14 can be a low-pass or band-pass filter, and the filter I14 is used for filtering out the part of exciting light which is overlapped with the fluorescence spectrum component of the sample and only transmitting fluorescence which can be effective on the sample. The mutual exclusion filter I15 is a pair of crossed linear polarizers placed between the filter I14 and the diaphragm I16, with the first polarizer at 90 deg. and the second polarizer positioned to block the reflected or scattered light, typically at a blocking rate higher than 99%.
The rear optical detection system is sequentially provided with a spherical lens 40, a diaphragm II 41, a mutual exclusion filter II 43, a filter II 42 and a lens III 44. The diaphragm II 41 functions to remove scattered light and fluorescence originating from the wall of the test tube, and to pass only fluorescence originating from the sample. The spherical lens 40 is added in front of the diaphragm II 41, so that the fluorescence in an angle of nearly 90 degrees can be collected and pass through the diaphragm II 41, the mutual exclusion filter II 43, the filter II 42 and the lens III 44 to reach the optical detector 50, and the lens III 44 is arranged in front of the optical detector 50.
The spherical lens 40 and the lens I12 form a 90-degree optical confocal system, the focuses of the two optical confocal systems coincide in a sample at the sample tube placing position 20, so that the high-efficiency collection of fluorescence components is achieved, the fluorescence collimated by the spherical lens 40 passes through the filter II 42 and the lens III 44, and the fluorescence is converged onto the optical detector 50 through the lens III 44. The optical filter II 42 is a dielectric film or an absorptive optical filter, and is used for removing exciting light and only transmitting fluorescent components, and the optical filter II 42 can also be a long-pass optical filter or a band-pass optical filter; the diaphragm II 41 functions to remove scattered light and fluorescence originating from the wall of the test tube, and to pass only fluorescence originating from the sample. The mutual exclusion filter ii 43 is a pair of crossed linear polarizers placed between filter ii 42 and lens iii 44, which is used in conjunction with the mutual exclusion filter i 15, and in particular the second polarizer at 90 ° of the first polarizer of the mutual exclusion filter ii 43 will block this reflected or scattered light, with blocking ratios typically higher than 99%.
When the portable optical detector works, light emitted by an excitation light source LED is converged into a sample tube at a sample tube placing position 20 through a lens I12, and before reaching a sample, the excitation light firstly passes through an optical filter I14 and a diaphragm I16; under the action of the excitation light, the fluorescence emitted from the sample passes through the diaphragm II 41, the spherical lens 40, the filter II 42 and the lens III 44, and finally reaches the optical detector 50. When light from the light source 10 is excited onto the sample cell, this configuration will greatly reduce background noise as a result of the reflected or scattered excitation light being largely linearly polarized in the original polarization direction, providing considerable optical isolation for the entire portable optical detector.
The analysis processing system comprises a CPU control circuit 60, a primary amplifier 61, a secondary amplifier 62, a digital-to-analog conversion circuit 65, a touch screen control circuit 67, a digital display circuit 68 and a USB interface circuit 69, wherein the optical detector 50 is connected with the CPU control circuit 60 through the primary amplifier 61, and the CPU control circuit 60 is connected with the light source 10 through the digital-to-analog conversion circuit 65 and the secondary amplifier 62 in sequence to regulate and control the light source 10. The touch screen control circuit 67, the digital display circuit 68 and the USB interface circuit 69 are respectively connected to the CPU control circuit 60 to perform a corresponding human-machine interaction function.
The utility model discloses above-mentioned portable optical detection instrument passes through spherical lens 40 and lens I12 and forms a 90 degrees confocal systems of optics to make the fluorescence that the sample sent can be by the collection of maximize, thereby increased the detectivity of portable optical detection instrument, as shown in fig. 2 and 3, wherein, fig. 2 is for using 365 nanometers to arouse the sensitivity of wavelength and verify the test result: the signal of the 25 pM fluorescent reagent is obvious and is equivalent to that of a desk-top fluorescence detector. FIG. 3 is the signal linearity verification test results using a 365 nm excitation wavelength: the signal remained linear from low concentration to 10000 pM.
Further, two sets of optical components can be placed in a mechanical housing, as shown in fig. 4, which is a schematic structural diagram of another embodiment of fig. 1, and by independently operating each optical component, the portable optical detector can be used for two different excitation wavelengths, such as 365 nm excitation wavelength and 450 nm excitation wavelength.
The above-mentioned embodiments, further detailed description of the objects, technical solutions and advantages of the present invention, it should be understood that the above description is only a detailed description of the present invention, and is not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. A portable optical detector, characterized in that: which comprises a preposed optical detection system, a sample test tube placing part (20), a postposition optical detection system and an analysis processing system,
a light source (10) is arranged in front of the front optical detection system, an optical detector (50) for receiving a fluorescence signal detected by the optical detection system is arranged behind the rear optical detection system, light emitted by the light source (10) is received by the optical detector (50) after passing through the front optical detection system, a sample test tube placing part (20) and the rear optical detection system, the front optical detection system sequentially comprises a lens I (12), an optical filter I (14), a mutual exclusion filter I (15) and a diaphragm I (16), and the light source (10) is arranged in front of the lens I (12);
the rear optical detection system is sequentially provided with a spherical lens (40), a diaphragm II (41), a mutual exclusion filter II (43), a filter II (42) and a lens III (44), and the lens III (44) is arranged in front of the optical detector (50); the spherical lens (40) collects the fluorescence within an angle of nearly 90 degrees, passes through a diaphragm II (41), a mutual exclusion filter II (43), and then passes through a filter II (42) and a lens III (44) to reach an optical detector (50); the spherical lens (40) and the lens I (12) form a 90-degree optical confocal system;
the analysis processing system comprises a CPU control circuit (60), a primary amplifier (61), a secondary amplifier (62), a digital-to-analog conversion circuit (65), a touch screen control circuit (67), a digital display circuit (68) and a USB interface circuit (69), wherein the optical detector (50) is connected with the CPU control circuit (60) through the primary amplifier (61), the CPU control circuit (60) is connected with the light source (10) sequentially through the digital-to-analog conversion circuit (65) and the secondary amplifier (62), and the touch screen control circuit (67), the digital display circuit (68) and the USB interface circuit (69) are respectively connected with the CPU control circuit (60).
2. The portable optical probe according to claim 1, wherein: the optical filter I (14) is a low-pass or band-pass optical filter.
3. The portable optical probe according to claim 1, wherein: the optical filter II (42) is a dielectric film or an absorptive optical filter.
4. The portable optical probe according to claim 1, wherein: the optical filter II (42) is a long-pass optical filter or a band-pass optical filter.
5. The portable optical probe according to any one of claims 1 to 4, wherein: the optical detector (50) is a silicon photodetector, an integrated photodetector, an avalanche photodiode detector or a photoelectric communication amplifier.
6. The portable optical probe according to any one of claims 1 to 4, wherein: the light source (10) is an LED as an excitation light source.
7. The portable optical probe according to any one of claims 1 to 4, wherein: the mutual exclusion filter II (43) is a pair of crossed linear polarizers.
8. The portable optical probe according to any one of claims 1 to 4, wherein: the mutual exclusion filter I (15) is a pair of crossed linear polarizers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020751116.XU CN212111141U (en) | 2020-05-09 | 2020-05-09 | Portable optical detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020751116.XU CN212111141U (en) | 2020-05-09 | 2020-05-09 | Portable optical detector |
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| CN212111141U true CN212111141U (en) | 2020-12-08 |
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| CN202020751116.XU Expired - Fee Related CN212111141U (en) | 2020-05-09 | 2020-05-09 | Portable optical detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111366569A (en) * | 2020-05-09 | 2020-07-03 | 杭州希浦芯光电科技有限公司 | Portable optical detector |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111366569A (en) * | 2020-05-09 | 2020-07-03 | 杭州希浦芯光电科技有限公司 | Portable optical detector |
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