CN202230024U - Fluorescence enhancement type optical fiber fluorescence probe - Google Patents
Fluorescence enhancement type optical fiber fluorescence probe Download PDFInfo
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- CN202230024U CN202230024U CN201120308836XU CN201120308836U CN202230024U CN 202230024 U CN202230024 U CN 202230024U CN 201120308836X U CN201120308836X U CN 201120308836XU CN 201120308836 U CN201120308836 U CN 201120308836U CN 202230024 U CN202230024 U CN 202230024U
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- sample cavity
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- 239000000523 sample Substances 0.000 title claims abstract description 53
- 239000013307 optical fiber Substances 0.000 title claims abstract description 43
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- 239000000835 fiber Substances 0.000 claims description 16
- 230000003287 optical effect Effects 0.000 claims description 13
- 239000007850 fluorescent dye Substances 0.000 claims description 12
- 238000005538 encapsulation Methods 0.000 claims description 4
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- 238000001514 detection method Methods 0.000 description 6
- 238000001917 fluorescence detection Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
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Abstract
The utility model relates to an optical fiber fluorescence probe specifically is a fluorescence enhancement mode optical fiber fluorescence probe. The device is characterized in that a plane reflector is arranged below the first collimating lens, a sample cavity is arranged below the second collimating lens, and the plane reflector is matched with the sample cavity; the right side of the sample cavity is provided with a first concave reflecting mirror, and a second concave reflecting mirror is arranged below the sample cavity. The utility model has the advantages that the probe adopts optical fiber (bundle) to transmit light, the structure is compact, the operation is flexible, and the separation of the photoelectric detector (spectrometer) and the sampling point is realized; the internal light path realizes the optimal separation of the excitation light and the fluorescence by using a collimating lens and a plane mirror; the built-in first concave reflector reflects the exciting light penetrating through the sample to be tested back to the sample to be tested, so that the aim of secondary excitation is fulfilled, and the excitation efficiency is improved; the second concave reflector reflects the back scattering fluorescence generated by the sample to be detected to enter the photoelectric detector, so that the fluorescence signal is enhanced, and the sensitivity is improved.
Description
Technical field
The utility model relates to a kind of optical fiber fluorescence probe, is specially a kind of fluorescence enhanced fiber fluorescent probe.
Background technology
Fluorescent spectroscopy is a secondary light emission of directly measuring material, have highly sensitive, selectivity good, advantage such as easy and simple to handle, has been widely used in scientific research and production fields such as environment, industry, agricultural, medical science at present.Traditional fluorescence spectrum instrument such as fluorospectrophotometer etc. are large-scale bench device; Need place sample cell just can carry out analysis to measure liquid sample during measurement; There are deficiencies such as complex structure, environment for use is limited, inconvenience is carried and cost an arm and a leg, can not satisfy on-the-spot the detection and the demand of monitoring in real time.
In recent years, because the development and the maturation of optical fiber and array type detector technology, make the spectral detection system form light source, sampling unit and take the photograph the version that the spectrum unit is separated that the total system structure has more modularization, uses convenient flexible.Optical fiber can replace traditional lens, catoptron, prism and complex optical system, as the light conduction path, optical energy loss is reduced, and light collecting light ability strengthens, and improves accuracy, the reliability of signal, further reduces the instrument volume and weight.The introducing of optical fiber also can change sample mode, makes spectral instrument break away from sample cell, utilizes the optical fiber measurement probe that exciting light is caused the detection position, gathers fluorescence simultaneously and passes instrument back, realizes that the online in real time of fluorescence spectrum is measured.But exciting light and the fluorescence coupling efficiency in optical fiber is generally lower, has certain loss simultaneously, thereby fluorescence excitation efficient and receiving efficiency are not high, and sensitivity is relatively low.
The utility model content
To the problem that exists in the prior art, the purpose of the utility model is to provide a kind of technical scheme of fluorescence enhanced fiber fluorescent probe.
Described a kind of fluorescence enhanced fiber fluorescent probe comprises incident optical, receives optical fiber, and incident optical one end connects the output terminal of excitation source, and the other end connects first fibre-optical splice, first collimation lens successively; Receive the input end that optical fiber one end connects photodetector; The other end connects second fibre-optical splice, second collimation lens successively; It is characterized in that described first collimation lens below is provided with plane mirror; Second collimation lens below is provided with sample cavity, and described plane mirror and sample cavity are equipped with; Described sample cavity right side is provided with first concave mirror, and the sample cavity below is provided with second concave mirror.
Described a kind of fluorescence enhanced fiber fluorescent probe is characterized in that described optical fiber fluorescence probe adopts epoxy encapsulation, at optical fiber fluorescence probe outer setting stainless steel sleeve pipe.
Described a kind of fluorescence enhanced fiber fluorescent probe is characterized in that described first collimation lens, second collimation lens all adopt the self-focusing Green lens.
Described a kind of fluorescence enhanced fiber fluorescent probe is characterized in that described incident optical and receives one group of fibre bundle that optical fiber is at least single big core diameter multimode optical fiber or many big core diameter multimode optical fiber compositions.
The advantage of the utility model is that probe adopts optical fiber (bundle) to pass light, compact conformation, and flexible operation, realization photodetector (spectrometer) separates with sampled point; Inner light path utilizes collimation lens, plane mirror to realize the optimal separation of exciting light and fluorescence; Built-in first concave mirror will be through the exciting light of testing sample reflected back testing sample again, thereby reaches the purpose of secondary excitation, improves launching efficiency; The backscattering fluorescence that the second concave reflection mirror reflection testing sample produces makes it get into photodetector, thereby strengthens fluorescence signal, improves sensitivity.
The utility model also has highly sensitive; Compact conformation, easy and simple to handle, carry convenient, cheap, be easy to advantages such as processing; Can be used for on-the-spot rapid fluorescence detection and online in real time monitoring, be specially adapted to fields such as environmental monitoring, Industry Control, chemical analysis, food quality detection, material analysis, clinical examination and education of science.
Description of drawings
Fig. 1 is the structural representation of the utility model;
Among the figure: 1-incident optical, 2-first fibre-optical splice, 3-first collimation lens, 4-plane mirror; The 5-sample cavity, 6-first concave mirror, 7-second concave mirror, 8-second collimation lens; 9-second fibre-optical splice, 10-receives optical fiber, 11-sleeve pipe, 12-excitation source; The 13-photodetector, 14-incident light excitation light path, 15-fluorescence detection light path, 16-epoxy resin.
Embodiment
Below in conjunction with Figure of description the utility model is further specified:
A kind of fluorescence enhanced fiber fluorescent probe; Comprise incident optical 1, receive optical fiber 10; Incident optical 1 is at least single big core diameter multimode optical fiber or many one group of fibre bundle that big core diameter multimode optical fiber is formed with reception optical fiber 10, and as the light conduction path, probe adopts big core diameter multimode optical fiber or fibre bundle conduction incident exciting light and emitting fluorescence; Realize that sampled point separates with photodetector (spectrometer), can sample automatically and online detection; Incident optical 1 one ends connect the output terminal of excitation source 12; The other end connects first fibre-optical splice 2, first collimation lens 3 successively; Receive the input end that optical fiber 10 1 ends connect photodetector 13; The other end connects second fibre-optical splice 9, second collimation lens, 8, the first collimation lenses 3, second collimation lens 8 successively and all adopts the self-focusing Green lens, can the divergent beams of incident be focused into parallel beam; Below first collimation lens 3, plane mirror 4 is set; Plane mirror 4 is with incident light turnover 90 degree; Make incident light excitation light path 14 vertical with fluorescence detection light path 15; Obtain the optimal separation of exciting light and fluorescence, improve signal to noise ratio (S/N ratio), avoid producing interference because of exciting light receives fluorescence signal to photodetector; Second collimation lens, 8 belows are provided with sample cavity 5; Described plane mirror 4 is equipped with sample cavity 5, guarantees can be radiated on the sample through plane mirror 4 light that comes out, and on sample cavity 5 right sides first concave mirror 6 is set; Sample cavity 5 belows are provided with second concave mirror 7; Promptly first concave mirror 6 is fixed in the end face of incident light excitation light path 14, and second concave mirror 7 is fixed in the end face of fluorescence detection light path 15, realizes the secondary excitation of testing sample and the collection of backscattering fluorescence; Improve fluorescence excitation efficient and receiving efficiency, strengthen fluorescence signal and sensitivity; The optical fiber fluorescence probe adopts epoxy resin 16 encapsulation, at optical fiber fluorescence probe outer setting stainless steel sleeve pipe 11, when supporting cover glass eyeglass and optical fiber, prevents the influence of extraneous parasitic light.
Principle of work: the output light of excitation source 12 gets into probe through first incident optical 1; Behind first collimation lens, 3 collimations, conduct to sample cavity 5 by plane mirror 4 turnovers 90 degree; Testing sample in the excited sample chamber produces fluorescence, and first concave mirror 6 will reflect the realization secondary excitation once more through the exciting light of sample simultaneously; Directive receives the fluorescence of optical fiber 10 behind second collimation lens, 8 collimations, is coupled into and receives optical fiber 10, and the fluorescence that receives fibre scattering is dorsad reflected by second concave mirror 7 after behind second collimation lens, 8 collimations, be coupled into and receive optical fiber 10; Receive fiber-optic output and link to each other with photodetector 13, fluorescence signal conducts to photodetector 13 receptions through receiving optical fiber 10.
The utlity model has following advantage:
1. probe adopts the right angle light channel structure, by first collimation lens, plane mirror with the exciting light collimation after turnover 90 degree, make the incident light excitation light path vertical with the fluorescence detection light path, avoid producing interference because of exciting light receives fluorescence signal to photodetector;
2. be separately fixed at incident light excitation light path end face, first concave mirror of fluorescence detection light path end face, first concave mirror; Can realize the secondary excitation of testing sample and the collection of backscattering fluorescence; Improve fluorescence excitation efficient and receiving efficiency, strengthen fluorescence signal and sensitivity;
3. probe adopts big core diameter multimode optical fiber or fibre bundle conduction incident exciting light and emitting fluorescence, realizes that sampled point separates with photodetector (spectrometer), can sample automatically and online detection;
4. outer sleeve adopts the stainless steel pipe of resistance to chemical attack, and with epoxy encapsulation, when supporting cover glass eyeglass and optical fiber, prevent extraneous parasitic light influence,
The utlity model has highly sensitive, compact conformation, good stability, easy and simple to handle, carry convenient, cheap, be easy to advantage such as processing, can be widely used in on-the-spot rapid fluorescence and detect.
Claims (4)
1. a fluorescence enhanced fiber fluorescent probe comprises incident optical, receives optical fiber, and incident optical one end connects the output terminal of excitation source, and the other end connects first fibre-optical splice, first collimation lens successively; Receive the input end that optical fiber one end connects photodetector; The other end connects second fibre-optical splice, second collimation lens successively; It is characterized in that described first collimation lens below is provided with plane mirror; Second collimation lens below is provided with sample cavity, and described plane mirror and sample cavity are equipped with; Described sample cavity right side is provided with first concave mirror, and the sample cavity below is provided with second concave mirror.
2. a kind of fluorescence enhanced fiber fluorescent probe according to claim 1 is characterized in that described optical fiber fluorescence probe adopts epoxy encapsulation, at optical fiber fluorescence probe outer setting stainless steel sleeve pipe.
3. a kind of fluorescence enhanced fiber fluorescent probe according to claim 1 is characterized in that described first collimation lens, second collimation lens all adopt the self-focusing Green lens.
4. a kind of fluorescence enhanced fiber fluorescent probe according to claim 1 is characterized in that described incident optical and receives one group of fibre bundle that optical fiber is at least single big core diameter multimode optical fiber or many big core diameter multimode optical fiber compositions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120308836XU CN202230024U (en) | 2011-08-24 | 2011-08-24 | Fluorescence enhancement type optical fiber fluorescence probe |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120308836XU CN202230024U (en) | 2011-08-24 | 2011-08-24 | Fluorescence enhancement type optical fiber fluorescence probe |
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| CN202230024U true CN202230024U (en) | 2012-05-23 |
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| CN201120308836XU Expired - Fee Related CN202230024U (en) | 2011-08-24 | 2011-08-24 | Fluorescence enhancement type optical fiber fluorescence probe |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102879096A (en) * | 2012-09-04 | 2013-01-16 | 深圳汉谱光彩科技有限公司 | System and method for measuring diffuse illumination reflection spectrum of dual-light path single grating |
| CN103335987A (en) * | 2013-06-26 | 2013-10-02 | 无锡微奥科技有限公司 | Fourier spectrograph based on micro electro mechanical system |
| CN103969229A (en) * | 2013-01-30 | 2014-08-06 | 全视科技有限公司 | Fluorescence imaging module |
| CN106018278A (en) * | 2016-07-11 | 2016-10-12 | 上海爱涛信息科技有限公司 | Miniaturized photoelectric module for fluorescence detection |
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN106706585A (en) * | 2016-12-31 | 2017-05-24 | 中国科学院昆明动物研究所 | Device and method for recording living body fluorescence signals |
| CN109100333A (en) * | 2018-09-21 | 2018-12-28 | 深圳市长隆科技有限公司 | Reflective probe and fluid on-line detector |
| CN109164080A (en) * | 2018-09-29 | 2019-01-08 | 江苏瑞明生物科技有限公司 | Suitable for the lossless fluorescence detection spectrometer of cellular level |
| CN109164078A (en) * | 2018-09-20 | 2019-01-08 | 苏州赛德福科学仪器有限公司 | A kind of phosphor collection device of fluorescence detector |
| CN109374583A (en) * | 2018-11-28 | 2019-02-22 | 南京清研六迪科学仪器有限公司 | A kind of online drug dissolution fibre optical sensor detection probe |
| CN109959591A (en) * | 2017-12-22 | 2019-07-02 | 日立-Lg数据存储韩国公司 | Combined dust sensor and gas sensor sensor |
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
| CN112557358A (en) * | 2020-11-25 | 2021-03-26 | 广东省测试分析研究所（中国广州分析测试中心） | Online fluorescence detection light path of water pollutant |
-
2011
- 2011-08-24 CN CN201120308836XU patent/CN202230024U/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102879096A (en) * | 2012-09-04 | 2013-01-16 | 深圳汉谱光彩科技有限公司 | System and method for measuring diffuse illumination reflection spectrum of dual-light path single grating |
| CN102879096B (en) * | 2012-09-04 | 2013-08-21 | 上海汉谱光电科技有限公司 | System and method for measuring diffuse illumination reflection spectrum of dual-light path single grating |
| CN103969229A (en) * | 2013-01-30 | 2014-08-06 | 全视科技有限公司 | Fluorescence imaging module |
| CN103969229B (en) * | 2013-01-30 | 2016-12-07 | 豪威科技股份有限公司 | Fluorescence imaging module |
| CN103335987A (en) * | 2013-06-26 | 2013-10-02 | 无锡微奥科技有限公司 | Fourier spectrograph based on micro electro mechanical system |
| CN106018278A (en) * | 2016-07-11 | 2016-10-12 | 上海爱涛信息科技有限公司 | Miniaturized photoelectric module for fluorescence detection |
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN106706585A (en) * | 2016-12-31 | 2017-05-24 | 中国科学院昆明动物研究所 | Device and method for recording living body fluorescence signals |
| CN109959591A (en) * | 2017-12-22 | 2019-07-02 | 日立-Lg数据存储韩国公司 | Combined dust sensor and gas sensor sensor |
| CN109164078A (en) * | 2018-09-20 | 2019-01-08 | 苏州赛德福科学仪器有限公司 | A kind of phosphor collection device of fluorescence detector |
| CN109100333A (en) * | 2018-09-21 | 2018-12-28 | 深圳市长隆科技有限公司 | Reflective probe and fluid on-line detector |
| CN109164080A (en) * | 2018-09-29 | 2019-01-08 | 江苏瑞明生物科技有限公司 | Suitable for the lossless fluorescence detection spectrometer of cellular level |
| CN109374583A (en) * | 2018-11-28 | 2019-02-22 | 南京清研六迪科学仪器有限公司 | A kind of online drug dissolution fibre optical sensor detection probe |
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
| CN112557358A (en) * | 2020-11-25 | 2021-03-26 | 广东省测试分析研究所（中国广州分析测试中心） | Online fluorescence detection light path of water pollutant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20150824 |
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| EXPY | Termination of patent right or utility model |