CN109164078A - A kind of phosphor collection device of fluorescence detector - Google Patents
A kind of phosphor collection device of fluorescence detector Download PDFInfo
- Publication number
- CN109164078A CN109164078A CN201811101103.1A CN201811101103A CN109164078A CN 109164078 A CN109164078 A CN 109164078A CN 201811101103 A CN201811101103 A CN 201811101103A CN 109164078 A CN109164078 A CN 109164078A
- Authority
- CN
- China
- Prior art keywords
- box body
- flow cell
- light
- detects
- slit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 11
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 230000005284 excitation Effects 0.000 claims abstract description 17
- 230000002776 aggregation Effects 0.000 claims abstract description 13
- 238000004220 aggregation Methods 0.000 claims abstract description 13
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000007689 inspection Methods 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002356 laser light scattering Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of phosphor collection devices of the fluorescence detector of high sensitivity, including detecting box body, it detects box body and is equipped with light entrance slit, entrance slit is externally provided with excitation monochrometer on detection box body, detect the first optically focused camera lens for being equipped with aggregation incident light in box body along optical path direction in light entrance slit, the flow cell for holding object to be checked is equipped in detection box body in the optical path of the first optically focused camera lens aggregation light, it detects in box body and assembles reflecting mirror equipped with exciting light relative on 180 ° of directions of excitation monochrometer in flow cell, assemble focus and is set to flow cell center, it detects in box body and is equipped with the second condenser lens head of aggregation emergent light in the beam projecting direction of flow cell, it detects and is equipped with beam projecting slit in the beam projecting position for second condenser lens head on box body, exit slit is externally provided with transmitting monochromator on detection box body, inspection It surveys in box body and assembles reflecting mirror relative to fluorescence is equipped on transmitting 180 ° of directions of monochromator in flow cell, aggregation focus is set to flow cell center.
Description
Technical field
The present invention relates to a kind of phosphor collection devices of fluorescence detector.
Background technique
The structure and working principle of the phosphor collection device of traditional fluorescence detector is as follows currently on the market: excitation is monochromatic
The monochromatic excitation light that device EX is generated is projected from the exit slit S1 of excitation monochromator, is gathered in flow cell FL through optically focused camera lens L1
Sample on.There is fluorescence generation as the sample in flow cell F is excited after light irradiation, with the excitation light direction being irradiated on sample
On the entrance slit S2 for gathering transmitting monochromator EM through optically focused camera lens L2 in the fluorescence in 90 ° of directions, into transmitting monochromator EM
Carry out tested sample be excited generation fluorescence intensity measurement.
Deficiency existing for the mechanism of this prevalence is: one-wavelength laser partially passes through flowing when being radiated at flow cell center
The laser light scattering of pond two sidewalls and sample causes exciting light energy to fail to make full use of.And sample is excited the major part of generation
Fluorescence is unutilized, only enters in transmitting monochromator EM with excitation light direction in 90 ° of part fluorescence and is detected, fluorescence is received
Collection rate is lower, influences the sensitivity of whole device.
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of phosphor collection dress of the fluorescence detector of high sensitivity
It sets.
In order to solve the above technical problems, the technical scheme adopted by the invention is as follows: a kind of phosphor collection of fluorescence detector
Device, including sealing and lighttight detection box body, detection box body are equipped with light entrance slit, detect entrance slit on box body
It is fixed with excitation monochrometer outside, detects poly- equipped with assemble incident light first along optical path direction in light entrance slit in box body
Light microscopic head, detection box body is interior to be equipped with the flow cell for holding object to be checked in the optical path of the first optically focused camera lens aggregation light, detects box
Assemble reflecting mirror relative to exciting light is equipped on 180 ° of directions of excitation monochrometer in flow cell in vivo, and assembles focus and be set to flowing
Pond center, the interior second condenser lens head that aggregation emergent light is equipped in the beam projecting direction of flow cell of detection box body, detects box body
On in the beam projecting position for second condenser lens head be equipped with beam projecting slit, detect on box body that fixation is set outside exit slit
There is transmitting monochromator, detection box body is interior to assemble reflecting mirror equipped with fluorescence relative on transmitting 180 ° of directions of monochromator in flow cell,
And aggregation focus is set to flow cell center.
As a preferred embodiment, the detection intracorporal flow cell of box is located at the exit focus of the first optically focused camera lens.
As a preferred embodiment, the beam projecting slit on the detection box body is located at the outgoing coke of second condenser lens head
At point.
The beneficial effects of the present invention are: the present apparatus assembles reflecting mirror due to using exciting light, the benefit of exciting light is improved
With rate, the intensity of fluorescence is generated to improve sample and be excited；Assemble reflecting mirror due to using fluorescence, to improve sample
Product be excited generation fluorescence collection efficiency；By improve above excitation light utilization efficiency with raising Fluorescence collection efficiency improve it is glimmering
The detection sensitivity of photodetector, optimizes signal-to-noise ratio.
Detailed description of the invention
Fig. 1 is the schematic illustration of conventional apparatus.
Fig. 2 is the principle of the present invention schematic diagram.
Specific embodiment
With reference to the accompanying drawing, detailed description of the present invention specific embodiment.
As shown in Fig. 2, a kind of phosphor collection device of fluorescence detector, including sealing and lighttight detection box body, inspection
It surveys box body and is equipped with light entrance slit S1, detect and be fixed with excitation monochrometer EX on box body outside entrance slit S1, detect box
It is equipped with the first optically focused camera lens L1 of aggregation incident light in body along optical path direction in light entrance slit S1, detection box body is interior first
It is equipped with the flow cell FL for holding object to be checked at exit focus in the optical path of optically focused camera lens L1 aggregation light, is being flowed in detection box body
Pond FL is opposite to assemble reflecting mirror M1 with installation exciting light on 180 ° of directions of excitation monochrometer S1, and assembles focus and be set to flow cell
The center FL, the interior second condenser lens head L2 that aggregation emergent light is equipped in the beam projecting direction of flow cell FL of detection box body, detection
It is equipped with beam projecting slit S2 in the beam projecting focal point for second condenser lens head L2 on box body, is emitted on detection box body narrow
Transmitting monochromator EM is fixed with outside seam S2, detection box body is interior on the opposite 180 ° of directions with transmitting monochromator EM flow cell FL
Fluorescence is installed and assembles reflecting mirror M2, and assembles focus and is set to the center flow cell FL.
When work: the monochromatic excitation light that exciting light monochromator EX is generated is projected from excitation monochromator exit slit S1, through poly-
Light microscopic head L1, gathers on the sample in flow cell FL, and exciting light aggregation reflecting mirror M1 will partially pass through the exciting light of flow cell
The center flow cell FL is reassembled, secondary excitation is carried out to sample.Have as the sample in flow cell FL is excited after light irradiation
Fluorescence generates, and fluorescence assembles reflecting mirror M2 and partial dispersion fluorescence reassembled the center flow cell FL, and glimmering on 90 ° of directions
Through optically focused camera lens L2 after optical superposition enhancing, on the entrance slit S2 that gathers transmitting monochromator EM, into transmitting monochromator EM into
Row sample be excited light generation fluorescence intensity measurement.
The principles and effects of the invention, and the implementation that part uses only is illustrated in the above embodiments
Example, and is not intended to limit the present invention；It should be pointed out that for those of ordinary skill in the art, not departing from wound of the present invention
Under the premise of making design, various modifications and improvements can be made, and these are all within the scope of protection of the present invention.
Claims (3)
1. a kind of phosphor collection device of fluorescence detector, it is characterised in that: including sealing and lighttight detection box body, detection
Box body is equipped with light entrance slit, detects and is fixed with excitation monochrometer on box body outside entrance slit, detects in box body in light
Line entrance slit is equipped with the first optically focused camera lens of aggregation incident light along optical path direction, assembles in detection box body in the first optically focused camera lens
The optical path of light is equipped with the flow cell for holding object to be checked, detects in box body in flow cell relative to 180 ° of directions of excitation monochrometer
It is equipped with exciting light and assembles reflecting mirror, and assemble focus and be set to flow cell center, detect the beam projecting in box body in flow cell
Direction is equipped with the second condenser lens head of aggregation emergent light, detects and sets on box body in the beam projecting position for second condenser lens head
There is beam projecting slit, detects and be fixed with transmitting monochromator on box body outside exit slit, it is opposite in flow cell in detection box body
Fluorescence is equipped in transmitting 180 ° of directions of monochromator and assembles reflecting mirror, and is assembled focus and be set to flow cell center.
2. a kind of phosphor collection device of fluorescence detector as described in claim 1, it is characterised in that: in the detection box body
Flow cell be located at the exit focus of the first optically focused camera lens.
3. a kind of phosphor collection device of fluorescence detector as claimed in claim 1 or 2, it is characterised in that: the detection box
Beam projecting slit on body is located at the exit focus of second condenser lens head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811101103.1A CN109164078A (en) | 2018-09-20 | 2018-09-20 | A kind of phosphor collection device of fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811101103.1A CN109164078A (en) | 2018-09-20 | 2018-09-20 | A kind of phosphor collection device of fluorescence detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109164078A true CN109164078A (en) | 2019-01-08 |
Family
ID=64879959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811101103.1A Pending CN109164078A (en) | 2018-09-20 | 2018-09-20 | A kind of phosphor collection device of fluorescence detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109164078A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10221244A (en) * | 1997-02-07 | 1998-08-21 | Tosoh Corp | Fluorescence detecting device |
| JP2003149154A (en) * | 2001-11-15 | 2003-05-21 | Hitachi High-Technologies Corp | Fluorescence spectrophotometer |
| US20060089554A1 (en) * | 2004-10-26 | 2006-04-27 | Olympus Corporation | Image generating device for generating a fluorescence image |
| CN1800829A (en) * | 2005-01-05 | 2006-07-12 | 中国科学院长春光学精密机械与物理研究所 | Optical structure of vacuum ultraviolet and fluorescence spectrum instrument |
| CN101915752A (en) * | 2010-07-05 | 2010-12-15 | 中国科学院深圳先进技术研究院 | Laser scanning imaging device |
| CN202230024U (en) * | 2011-08-24 | 2012-05-23 | 中国计量学院 | Fluorescence enhancement type optical fiber fluorescent probe |
| CN108426864A (en) * | 2017-11-21 | 2018-08-21 | 苏州赛德福科学仪器有限公司 | A kind of fluorescence excitation device of fluorescence detector |
| CN208766107U (en) * | 2018-09-20 | 2019-04-19 | 苏州赛德福科学仪器有限公司 | A kind of phosphor collection device of fluorescence detector |
-
2018
- 2018-09-20 CN CN201811101103.1A patent/CN109164078A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10221244A (en) * | 1997-02-07 | 1998-08-21 | Tosoh Corp | Fluorescence detecting device |
| JP2003149154A (en) * | 2001-11-15 | 2003-05-21 | Hitachi High-Technologies Corp | Fluorescence spectrophotometer |
| US20060089554A1 (en) * | 2004-10-26 | 2006-04-27 | Olympus Corporation | Image generating device for generating a fluorescence image |
| CN1800829A (en) * | 2005-01-05 | 2006-07-12 | 中国科学院长春光学精密机械与物理研究所 | Optical structure of vacuum ultraviolet and fluorescence spectrum instrument |
| CN101915752A (en) * | 2010-07-05 | 2010-12-15 | 中国科学院深圳先进技术研究院 | Laser scanning imaging device |
| CN202230024U (en) * | 2011-08-24 | 2012-05-23 | 中国计量学院 | Fluorescence enhancement type optical fiber fluorescent probe |
| CN108426864A (en) * | 2017-11-21 | 2018-08-21 | 苏州赛德福科学仪器有限公司 | A kind of fluorescence excitation device of fluorescence detector |
| CN208766107U (en) * | 2018-09-20 | 2019-04-19 | 苏州赛德福科学仪器有限公司 | A kind of phosphor collection device of fluorescence detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8427641B2 (en) | Compact detector for simultaneous particle size and fluorescence detection | |
| US9134230B2 (en) | Microbial detection apparatus and method | |
| CN103134780B (en) | A kind of detector of fluorescence induced by light-emitting diode of close contact excitation light path | |
| JP4259762B2 (en) | Optical configuration for detecting light | |
| CN107209102B (en) | Optical detection system and method of use | |
| JP2009529134A (en) | Apparatus and method for measuring photoluminescence, absorbance, and diffraction of fine objects in a fluid | |
| CN208766107U (en) | A kind of phosphor collection device of fluorescence detector | |
| JP2017138223A (en) | Minute object detector | |
| CN107561042A (en) | A kind of spot shaping optical system for fluorescence analyser | |
| CN206074449U (en) | A kind of spot shaping optical system for fluorescence analyser | |
| US9086377B2 (en) | Optical system for fluorescence detection and fine particle analyzing apparatus | |
| CN104597011B (en) | Excitation light source drift correction device and XRF | |
| CN207946357U (en) | A kind of fluorescence excitation device of fluorescence detector | |
| CN109164078A (en) | A kind of phosphor collection device of fluorescence detector | |
| CN103033464A (en) | Photoacoustic-fluorescence flow cytometer | |
| RU2448340C1 (en) | Method for optical detection of fluorescence and scattering signals of aerosol particles in stream and optical system for realising said method | |
| JP4763159B2 (en) | Flow cytometer | |
| CN115096795A (en) | Flow type fluorescence detection optical system | |
| JP2021503608A (en) | Optical flow cytometer for epifluorescence measurement | |
| JP6129014B2 (en) | Fluorescence detection apparatus and fluorescence detection method | |
| JPH03214038A (en) | Instrument for measuring aerosol, dust and the like spreaded in the air | |
| CN216411078U (en) | Immunofluorescence analyzer | |
| CN218767558U (en) | Confocal optical system of single-molecule detection immunoassay analyzer based on LD | |
| CN115598105B (en) | Focusing method and focusing system for Raman detection | |
| CN219142597U (en) | Single molecule detection immunoassay appearance optical system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |