CN208766107U - A kind of phosphor collection device of fluorescence detector - Google Patents

A kind of phosphor collection device of fluorescence detector Download PDF

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Publication number
CN208766107U
CN208766107U CN201821544063.3U CN201821544063U CN208766107U CN 208766107 U CN208766107 U CN 208766107U CN 201821544063 U CN201821544063 U CN 201821544063U CN 208766107 U CN208766107 U CN 208766107U
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China
Prior art keywords
box body
flow cell
light
detects
slit
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Active
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CN201821544063.3U
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Chinese (zh)
Inventor
姚凡
姚冬
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Suzhou Telford Scientific Instrument Co Ltd
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Suzhou Telford Scientific Instrument Co Ltd
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Priority to CN201821544063.3U priority Critical patent/CN208766107U/en
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Abstract

The utility model discloses a kind of phosphor collection devices of the fluorescence detector of high sensitivity, including detecting box body, it detects box body and is equipped with light entrance slit, entrance slit is externally provided with excitation monochrometer on detection box body, detect the first optically focused camera lens for being equipped with aggregation incident light in box body along optical path direction in light entrance slit, the flow cell for holding object to be checked is equipped in detection box body in the optical path of the first optically focused camera lens aggregation light, it detects in box body and assembles reflecting mirror equipped with exciting light relative on 180 ° of directions of excitation monochrometer in flow cell, assemble focus and is set to flow cell center, it detects in box body and is equipped with the second condenser lens head of aggregation emergent light in the beam projecting direction of flow cell, it detects and is equipped with beam projecting slit in the beam projecting position for second condenser lens head on box body, exit slit is externally provided with transmitting monochromator on detection box body , detection box body is interior to assemble reflecting mirror relative to fluorescence is equipped on transmitting 180 ° of directions of monochromator in flow cell, and aggregation focus is set on flow cell center.

Description

A kind of phosphor collection device of fluorescence detector
Technical field
The utility model relates to a kind of phosphor collection devices of fluorescence detector.
Background technique
The structure and working principle of the phosphor collection device of traditional fluorescence detector is as follows currently on the market: excitation is monochromatic The monochromatic excitation light that device EX is generated is projected from the exit slit S1 of excitation monochromator, is gathered in flow cell FL through optically focused camera lens L1 Sample on.There is fluorescence generation as the sample in flow cell F is excited after light irradiation, with the excitation light direction being irradiated on sample On the entrance slit S2 for gathering transmitting monochromator EM through optically focused camera lens L2 in the fluorescence in 90 ° of directions, into transmitting monochromator EM Carry out tested sample be excited generation fluorescence intensity measurement.
Deficiency existing for the mechanism of this prevalence is: one-wavelength laser partially passes through flowing when being radiated at flow cell center The laser light scattering of pond two sidewalls and sample causes exciting light energy to fail to make full use of.And sample is excited the major part of generation Fluorescence is unutilized, only enters in transmitting monochromator EM with excitation light direction in 90 ° of part fluorescence and is detected, fluorescence is received Collection rate is lower, influences the sensitivity of whole device.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of phosphor collections of the fluorescence detector of high sensitivity Device.
In order to solve the above technical problems, the technology employed by the present utility model is a kind of fluorescence of fluorescence detector Collection device, including sealing and lighttight detection box body, detection box body are equipped with light entrance slit, detect incident on box body It is fixed with excitation monochrometer outside slit, detects in box body and is equipped with the of aggregation incident light along optical path direction in light entrance slit One optically focused camera lens, detection box body is interior to be equipped with the flow cell for holding object to be checked, inspection in the optical path of the first optically focused camera lens aggregation light It surveys in box body and assembles reflecting mirror relative to exciting light is equipped on 180 ° of directions of excitation monochrometer in flow cell, and assemble focus and be set to Flow cell center, the interior second condenser lens head that aggregation emergent light is equipped in the beam projecting direction of flow cell of detection box body, detection It is equipped with beam projecting slit in the beam projecting position for second condenser lens head on box body, is detected solid outside exit slit on box body It is fixed to be equipped with transmitting monochromator, it detects in box body and is reflected in flow cell relative to fluorescence aggregation is equipped on transmitting 180 ° of directions of monochromator Mirror, and assemble focus and be set to flow cell center.
As a preferred embodiment, the detection intracorporal flow cell of box is located at the exit focus of the first optically focused camera lens.
As a preferred embodiment, the beam projecting slit on the detection box body is located at the outgoing coke of second condenser lens head At point.
The beneficial effects of the utility model are: the present apparatus assembles reflecting mirror due to using exciting light, exciting light is improved Utilization rate, generate the intensity of fluorescence to improve sample and be excited;Assemble reflecting mirror due to using fluorescence, to improve Sample be excited generation fluorescence collection efficiency;It is improved by improving excitation light utilization efficiency above and improving Fluorescence collection efficiency The detection sensitivity of fluorescence detector, optimizes signal-to-noise ratio.
Detailed description of the invention
Fig. 1 is the schematic illustration of conventional apparatus.
Fig. 2 is the principles of the present invention schematic diagram.
Specific embodiment
With reference to the accompanying drawing, the specific embodiment of the utility model is described in detail.
As shown in Fig. 2, a kind of phosphor collection device of fluorescence detector, including sealing and lighttight detection box body, inspection It surveys box body and is equipped with light entrance slit S1, detect and be fixed with excitation monochrometer EX on box body outside entrance slit S1, detect box It is equipped with the first optically focused camera lens L1 of aggregation incident light in body along optical path direction in light entrance slit S1, detection box body is interior first It is equipped with the flow cell FL for holding object to be checked at exit focus in the optical path of optically focused camera lens L1 aggregation light, is being flowed in detection box body Pond FL is opposite to assemble reflecting mirror M1 with installation exciting light on 180 ° of directions of excitation monochrometer S1, and assembles focus and be set to flow cell The center FL, the interior second condenser lens head L2 that aggregation emergent light is equipped in the beam projecting direction of flow cell FL of detection box body, detection It is equipped with beam projecting slit S2 in the beam projecting focal point for second condenser lens head L2 on box body, is emitted on detection box body narrow Transmitting monochromator EM is fixed with outside seam S2, detection box body is interior on the opposite 180 ° of directions with transmitting monochromator EM flow cell FL Fluorescence is installed and assembles reflecting mirror M2, and assembles focus and is set to the center flow cell FL.
When work: the monochromatic excitation light that exciting light monochromator EX is generated is projected from excitation monochromator exit slit S1, through poly- Light microscopic head L1, gathers on the sample in flow cell FL, and exciting light aggregation reflecting mirror M1 will partially pass through the exciting light of flow cell The center flow cell FL is reassembled, secondary excitation is carried out to sample.Have as the sample in flow cell FL is excited after light irradiation Fluorescence generates, and fluorescence assembles reflecting mirror M2 and partial dispersion fluorescence reassembled the center flow cell FL, and glimmering on 90 ° of directions Through optically focused camera lens L2 after optical superposition enhancing, on the entrance slit S2 that gathers transmitting monochromator EM, into transmitting monochromator EM into Row sample be excited light generation fluorescence intensity measurement.
The principles and effects of the invention, and the implementation that part uses only is illustrated in the above embodiments Example, rather than limits the present invention;It should be pointed out that for those of ordinary skill in the art, not departing from this hair Under the premise of bright concept, various modifications and improvements can be made, and these are all within the protection scope of the present invention.

Claims (3)

1. a kind of phosphor collection device of fluorescence detector, it is characterised in that: including sealing and lighttight detection box body, detection Box body is equipped with light entrance slit, detects and is fixed with excitation monochrometer on box body outside entrance slit, detects in box body in light Line entrance slit is equipped with the first optically focused camera lens of aggregation incident light along optical path direction, assembles in detection box body in the first optically focused camera lens The optical path of light is equipped with the flow cell for holding object to be checked, detects in box body in flow cell relative to 180 ° of directions of excitation monochrometer It is equipped with exciting light and assembles reflecting mirror, and assemble focus and be set to flow cell center, detect the beam projecting in box body in flow cell Direction is equipped with the second condenser lens head of aggregation emergent light, detects and sets on box body in the beam projecting position for second condenser lens head There is beam projecting slit, detects and be fixed with transmitting monochromator on box body outside exit slit, it is opposite in flow cell in detection box body Fluorescence is equipped in transmitting 180 ° of directions of monochromator and assembles reflecting mirror, and is assembled focus and be set to flow cell center.
2. a kind of phosphor collection device of fluorescence detector as described in claim 1, it is characterised in that: in the detection box body Flow cell be located at the exit focus of the first optically focused camera lens.
3. a kind of phosphor collection device of fluorescence detector as claimed in claim 1 or 2, it is characterised in that: the detection box Beam projecting slit on body is located at the exit focus of second condenser lens head.
CN201821544063.3U 2018-09-20 2018-09-20 A kind of phosphor collection device of fluorescence detector Active CN208766107U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821544063.3U CN208766107U (en) 2018-09-20 2018-09-20 A kind of phosphor collection device of fluorescence detector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821544063.3U CN208766107U (en) 2018-09-20 2018-09-20 A kind of phosphor collection device of fluorescence detector

Publications (1)

Publication Number Publication Date
CN208766107U true CN208766107U (en) 2019-04-19

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CN201821544063.3U Active CN208766107U (en) 2018-09-20 2018-09-20 A kind of phosphor collection device of fluorescence detector

Country Status (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109164078A (en) * 2018-09-20 2019-01-08 苏州赛德福科学仪器有限公司 A kind of phosphor collection device of fluorescence detector
CN111781182A (en) * 2020-07-23 2020-10-16 浙江富昇科技有限公司 Portable fluorescence detection device for detecting heavy metal ions in water

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109164078A (en) * 2018-09-20 2019-01-08 苏州赛德福科学仪器有限公司 A kind of phosphor collection device of fluorescence detector
CN111781182A (en) * 2020-07-23 2020-10-16 浙江富昇科技有限公司 Portable fluorescence detection device for detecting heavy metal ions in water

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