CN207946357U - A kind of fluorescence excitation device of fluorescence detector - Google Patents
A kind of fluorescence excitation device of fluorescence detector Download PDFInfo
- Publication number
- CN207946357U CN207946357U CN201820469068.8U CN201820469068U CN207946357U CN 207946357 U CN207946357 U CN 207946357U CN 201820469068 U CN201820469068 U CN 201820469068U CN 207946357 U CN207946357 U CN 207946357U
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- light
- box body
- flow cell
- concave grating
- plane mirror
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The utility model discloses a kind of simple in structure,The fluorescence excitation device of the low fluorescence detector of manufacturing cost,Including closed lighttight detection box body,It detects box body and is equipped with light entrance slit,It detects and is equipped with the first plane mirror in box body along optical path direction in light entrance slit,It detects and is equipped with concave grating in box body in the light path of the first plane mirror reflection light,In detection box body the second plane mirror being arranged on the silicon photocell in detecting box body is reflexed to equipped with some light that will be above flow cell light portal upper edge after concave grating dispersion in the light path of light,The flow cell for holding object to be checked is equipped in the light path of light after concave grating dispersion in detection box body,Flow cell is equipped with light portal and light outlet,Light belt width at flow cell after concave grating dispersion is more than the effective dimensions of light portal,It detects in box body and is equipped with visible light-focusing camera lens in the beam projecting direction of flow cell,It detects box body and is equipped with beam projecting slit.
Description
Technical field
The utility model is related to a kind of fluorescence excitation devices of fluorescence detector.
Background technology
Currently used fluorescence detector uses xenon lamp to have fluorescence property in object to be checked as excitation light source to excite
Substance generate fluorescence, and then analyzed by monochromator.Since xenon lamp luminous intensity is unstable, and in fluorescence excitation
When, if incident intensity fluctuates, the effect of fluoroscopic examination can be influenced, so need to be detected the intensity of incident light, with
Ensure the reliability of fluorescence detector testing result.
Incident intensity monitoring generally uses silicon photocell, and silicon photocell itself is more sensitive to light intensity, if be irradiated
Degree can make silicon photocell enter nonlinear operation area, or even make silicon photocell blinding, so general fluorescence excitation apparatus structure is such as
Shown in Fig. 1, the light that xenon source is sent out is reflected through plane mirror M, is projected on grating G by slit S1, through grating G points
After light, monochromatic light is generated by slit S2；The monochromatic light passes through is divided into two-way light with the beam splitter BS of optical axis direction installation at 45 °
Beam.Monochromatic light all the way across beam splitter BS passes through condenser L1, focuses on flow cell FL, if had in flow cell at this time glimmering
Stimulative substance, then fluorescent material be inspired fluorescence, focused on slit S3 by condenser L2, into transmitting monochromator carry out it is glimmering
Luminous intensity measurement.Another way monochromatic light by beam splitter reflection is focused through condenser L3, through multilayer dim light net SH, by monochromatic light
It is attenuated in silicon photocell linear working range, on the light-receiving surface for projecting silicon photocell PD, to be sent out to xenon source
Light energy fluctuating change monitoring.
Deficiency is existing for this structure：
1. when carrying out fluorescence measurement, it is desirable to which the energy for being irradiated to the monochromatic excitation light of tested fluorescent material is sufficiently strong.It is existing
Light path in be provided with beam splitter after, monochromatic excitation light is separated a part (generally 50%), is irradiated on sample
Monochromatic light is weakened, and reduces the utilization rate of energy of light source, reduces the measurement sensitivity of instrument.
It is uneven 2. the operation wavelength of instrument, within the scope of 200nm~800nm, the variation of the splitting ratio of beam splitter is very big
Even.So measurement sensitivity in entire wave-length coverage is also variation, and work in the beam splitter of this wave-length coverage
Price is also expensive.
3. the monochromatic light projected from slit is diverging, flow cell and silicon light need to be projected respectively after condenser focuses
On battery.Because monochromatic light is after condenser focuses, energy will generate loss, further reduced the utilization rate of energy of light source,
Also it further reduced apparatus measures sensitivity.Equally, ultraviolet condenser of the operation wavelength within the scope of 200nm~800nm
Price is also more expensive.
4. such structure is more complex, of high cost；So that the debugging of instrument complete machine, collimation technique is complicated.
Utility model content
Technical problem to be solved in the utility model is：A kind of fluoroscopic examination simple in structure, manufacturing cost is low is provided
The fluorescence excitation device of device.
In order to solve the above technical problems, technical solution used by the utility model is：A kind of fluorescence of fluorescence detector
Excitation apparatus, including closed lighttight detection box body, detection box body are equipped with light entrance slit, detect in box body in light
Line entrance slit is equipped with the first plane mirror of reflection incident light along optical path direction, and detection box body is interior in the first plane mirror reflected light
The light path of line is equipped with concave grating, is equipped in the light path of light after concave grating dispersion in detection box body and will be above flow cell
The some light of light portal upper edge reflexes to the second plane mirror being arranged on the silicon photocell in detecting box body, detection
It is equipped with the flow cell for holding object to be checked in box body in the light path of light after concave grating dispersion, flow cell is equipped with light portal
And light outlet, the light belt width at flow cell after concave grating dispersion are more than the effective dimensions of light portal, detect in box body
It is equipped with visible light-focusing camera lens in the beam projecting direction of flow cell, is detected on box body in the beam projecting corresponding to optically focused camera lens
Position is equipped with beam projecting slit.
As a preferred solution, the reflection angle of second plane mirror is adjustable.
As another preferred scheme, second plane mirror is adjustable relative to the distance of silicon photocell.
As a preferred solution, in the concave grating the second plane mirror of the heart channel of Hang-Shaoyin to silicon photocell light-receiving surface
Distance is with the light portal at concave grating center to flow cell apart from equal.
As a preferred solution, the beam projecting slit on the detection box body is located at the exit focus of optically focused camera lens
Place.
The utility model has the beneficial effects that：Due to being equipped in the light path of light after concave grating dispersion in detection box body
Will be above some light of flow cell light portal upper edge, to reflex to second be arranged on the silicon photocell in detecting box body flat
Face reflective mirror eliminates the beam splitting in 200nm~800nm operating wavelength ranges after replacing beam splitter with common plane speculum
Instrument caused by the large change of mirror is in the variation for entirely measuring the measurement sensitivity on wave band；It has cut expensive ultraviolet
Condenser L1, L3, beam splitter BS and slit B2 and dim light net and with the relevant structure of above-mentioned component, simplify structure, reduce
Cost simplifies debugging, collimation technique.
The flow cell of object to be checked, such as instrument are held due to being equipped in the light path of light after concave grating dispersion in detection box body
Device keeps measurement sensitivity same as Fig. 1 mechanisms can be very big since source light just shines directly into flow cell after grating
Ground reduces light source power, reduces complete machine power and Wen Sheng, is conducive to the raising of stability of instrument and the reduction of cost.
Since the reflection angle of the second plane mirror is adjustable, it can be adjusted and be reflexed on silicon photocell according to service condition
Amount light, so that silicon photocell is operated in linear working range.
Due to distance and concave grating center of the second plane mirror of the heart channel of Hang-Shaoyin to silicon photocell light-receiving surface in concave grating
To flow cell light portal apart from equal, can more accurately measure the incident intensity for projecting flow cell.
Description of the drawings
Fig. 1 is the principle schematic of conventional apparatus.
Fig. 2 is the principle schematic of the present apparatus.
Fig. 3 is the position view that direction concave grating and the second plane mirror and flow cell are looked up in Fig. 2.
Specific implementation mode
Below in conjunction with the accompanying drawings, the specific embodiment of the utility model is described in detail.
As Figure 2-3, the fluorescence excitation device of a kind of fluorescence detector, including closed lighttight detection box body,
Detect box body be equipped with light entrance slit S1, detect box body in light entrance slit S1 along optical path direction be equipped with reflect into
The first plane mirror M1 of light is penetrated, concave grating G is equipped in the light path of the first plane mirror M1 reflection lights in detection box body.
It is equipped in the light path of light after concave grating G dispersions in detection box body and will be above flow cell light portal upper edge
Some light reflexes to the adjustable second plane mirror M2 of the reflection angle being arranged on the silicon photocell PD in detecting box body.
Second plane mirror M2 is adjustable relative to the distance of silicon photocell PD.Detect the interior light after concave grating G dispersions of box body
Light path is equipped with the flow cell FL for holding object to be checked.Flow cell FL is equipped with light portal and light outlet, recessed at flow cell FL
Light belt width after concave grating dispersion is more than the effective dimensions of light portal, and the second plane of the heart channel of Hang-Shaoyin is reflective in the concave grating G
Mirror M2 to silicon photocell PD light-receiving surfaces distance with the light portal at the centers concave grating G to flow cell FL apart from equal.
It detects in box body and is equipped with visible light-focusing camera lens L2 in the beam projecting direction of flow cell FL, detect on box body right
It should be equipped with beam projecting slit S3 in the beam projecting position of optically focused camera lens L2.Beam projecting slit S3 on detection box body is located at
At the exit focus of optically focused camera lens L2.
The light that xenon source is sent out projects on excitation grating G by slit S1 through plane mirror M1 reflections, through G points
It after light, directly focuses on flow cell FL, at this point, if there is fluorescent material in flow cell, fluorescent material is inspired glimmering
Light is focused on through condenser L2 on slit S3, and the measurement of fluorescence intensity is carried out into incident monochromator.
And the light except flow cell FL light portal vertical direction upper edges reflexes to silicon light through the second plane mirror M2
On battery PD, to the monitoring of the fluctuating change of the light energy sent out to xenon source.Up and down adjustment plane mirror M2 so that throw
The monochromatic light energy on silicon photocell PD is mapped in the linear working range of silicon photocell.
The principles and effects of the utility model creation are only illustrated in the above embodiments, and the reality that part uses
Example is applied, is rather than limited the present invention；It should be pointed out that for those of ordinary skill in the art, not departing from this
Under the premise of utility model concept, various modifications and improvements can be made, these belong to the protection of the utility model
Range.
Claims (5)
1. a kind of fluorescence excitation device of fluorescence detector, including closed lighttight detection box body, detection box body is equipped with
Light entrance slit detects the first plane mirror for being equipped with reflection incident light in box body along optical path direction in light entrance slit,
It detects and is equipped with concave grating in box body in the light path of the first plane mirror reflection light, it is characterised in that：In the detection box body
It is equipped with to reflex to some light in the light path of light after concave grating dispersion and be arranged on the silicon photocell in detecting box body
The second plane mirror, be equipped in the light path of light after concave grating dispersion in detection box body and hold the flowing of object to be checked
Pond, flow cell are equipped with light portal and light outlet, and the light belt width at flow cell after concave grating dispersion is more than light inlet window
Mouthful effective dimensions, detection box body in the beam projecting direction of flow cell be equipped with visible light-focusing camera lens, detect box body on
It is equipped with beam projecting slit corresponding to the beam projecting position of optically focused camera lens.
2. a kind of fluorescence excitation device of fluorescence detector as described in claim 1, it is characterised in that：Second plane is anti-
The reflection angle of light microscopic is adjustable.
3. a kind of fluorescence excitation device of fluorescence detector as claimed in claim 2, it is characterised in that：Second plane is anti-
Light microscopic is adjustable relative to the distance of silicon photocell.
4. a kind of fluorescence excitation device of fluorescence detector as claimed in claim 2 or claim 3, it is characterised in that：The concave surface light
Light portal of the second plane mirror of the heart channel of Hang-Shaoyin to the distance and concave grating center to flow cell of silicon photocell light-receiving surface in grid
Apart from equal.
5. a kind of fluorescence excitation device of fluorescence detector as claimed in claim 4, it is characterised in that：On the detection box body
Beam projecting slit be located at the exit focus of optically focused camera lens.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711165754 | 2017-11-21 | ||
| CN2017111657542 | 2017-11-21 |
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| CN207946357U true CN207946357U (en) | 2018-10-09 |
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| CN201810292949.1A Pending CN108426864A (en) | 2017-11-21 | 2018-03-30 | A kind of fluorescence excitation device of fluorescence detector |
| CN201820469068.8U Active CN207946357U (en) | 2017-11-21 | 2018-03-30 | A kind of fluorescence excitation device of fluorescence detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426864A (en) * | 2017-11-21 | 2018-08-21 | 苏州赛德福科学仪器有限公司 | A kind of fluorescence excitation device of fluorescence detector |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164078A (en) * | 2018-09-20 | 2019-01-08 | 苏州赛德福科学仪器有限公司 | A kind of phosphor collection device of fluorescence detector |
| CN114527227B (en) * | 2022-02-23 | 2023-10-13 | 上海天美科学仪器有限公司 | Flow cell assembly for cross passing of composite light and monochromatic light |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS6324139A (en) * | 1986-03-13 | 1988-02-01 | Olympus Optical Co Ltd | Measuring method for particle size |
| US5176728A (en) * | 1991-09-24 | 1993-01-05 | Cambrian Systems, Inc. | Method of making a mirror having extremely small aperture holes at other than normal angles to the surfaces of the mirror |
| US6628385B1 (en) * | 1999-02-05 | 2003-09-30 | Axon Instruments, Inc. | High efficiency, large field scanning microscope |
| CN202267653U (en) * | 2011-10-09 | 2012-06-06 | 天津陆海石油设备系统工程有限责任公司 | Three-dimensional fluorescence analyzer |
| CN105393104B (en) * | 2013-07-23 | 2020-08-14 | 索尼公司 | Particle analysis device and particle analysis method |
| KR101613883B1 (en) * | 2015-12-03 | 2016-05-02 | 기초과학연구원 | An amplitude and phase analysis device of the light wave |
| CN108426864A (en) * | 2017-11-21 | 2018-08-21 | 苏州赛德福科学仪器有限公司 | A kind of fluorescence excitation device of fluorescence detector |
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| CN108426864A (en) * | 2017-11-21 | 2018-08-21 | 苏州赛德福科学仪器有限公司 | A kind of fluorescence excitation device of fluorescence detector |
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