CN201689050U - Fluophotometer capable of detecting light absorption or light transmission - Google Patents
Fluophotometer capable of detecting light absorption or light transmission Download PDFInfo
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- CN201689050U CN201689050U CN2009201762454U CN200920176245U CN201689050U CN 201689050 U CN201689050 U CN 201689050U CN 2009201762454 U CN2009201762454 U CN 2009201762454U CN 200920176245 U CN200920176245 U CN 200920176245U CN 201689050 U CN201689050 U CN 201689050U
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- light
- fluorophotometer
- sample
- fluorescence
- fluophotometer
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Abstract
The utility model provides a fluophotometer capable of detecting light absorption or light transmission, comprising a light source, an exciting light monochromator, a sample tank, an emission light monochromator and a detector; the fluophotometer is characterized in that: the fluophotometer further comprises a reflecting system which leads the transmission light of the sample to reflect to the detector of the original fluophotometer, and the detector detects the transmission ratio of the sample to the light; the fluophotometer is improved on the basis of the fluophotometer in prior art, the fluorescence detection and light absorption (light transmission) detection are combined, so as to achieve the purposes of detecting the sample fluorescence (including three-dimensional fluorescence and synchronization fluorescence) spectrums and light adsorption spectrums (such as absorbance or light transmittance and the like) of the sample; meanwhile, the fluophotometer can realize correction of the three-dimensional fluorescence spectrum conveniently.
Description
Technical field
The present invention protects and a kind ofly can detect light absorption or light transmissive fluorophotometer.This fluorophotometer belongs to a kind of joint-detection instrument, and described detector comprises ultraviolet/visible spectrophotometer and two parts of fluorophotometer, and this detector can be realized two kinds of functions of ultraviolet/visible spectrophotometer and fluorophotometer.Simultaneously, this photometer can be realized the rectification to three-dimensional fluorescence spectrum easily.
Background technology
Three-dimensional fluorescence spectrum is a kind of new analytical technology that grows up on the basis of fluorescent spectroscopy the eighties in 20th century, the method of obtaining the three-dimensional fluorescence data generally is a continuous sweep emission spectrum on different excitation wavelengths position, and can utilize various mapping softwares that it is showed with isogonism three-dimensional fluorescence perspective view (Ex-Em-If) or contour map form image conversions such as (Ex-Em).Using fluorescence spectrum technical research fluorescence class material is based on and contains the aromatic ring structure that has various functional groups in a large number and unsaturation aliphatic chain in its structure (why material has fluorescence is owing to have low-yield п → п in its structure
*The aromatic structure of transition or conjugation chromophore).Research methods such as infrared relatively, nuclear magnetic resonance, fluorescent spectrometry research natural organic matter architectural feature has highly sensitive (10
-9The order of magnitude), selectivity is good, the sample size that do not destroy sample structure, needs is few (only to need a small amount of low concentration, usually＜water sample of 20mg/L), sample do not need special separation, advantage such as quick and easy, be used to the qualitative or quantitative description of organic matter or soil ulmin structure and functional group, and in organic source and the water monitoring research.
Yet, in fluoroscopic examination, common inner filtration effect and self-absorption phenomenon owing to solution, and cause measured fluorescent weakening, make fluorescence intensity and peak type all change.Along with the increase of sample concentration, the inner filtration effect and the self-absorption phenomenon of solution are serious especially, to such an extent as to fluoroscopic examination occurs than large deviation.The Wang Yana of Dalian Polytechnic University, Cui Li, Xu Tongkuan, Luan Qiang etc. are at " applied research of three-dimensional fluorescence spectrum " (assay office, 2008-5, V27 supplementary issue, 59-62 page or leaf) just points out in the literary composition, when rhodamine B being detected with three-dimensional fluorescence, " may be because produce the fluorescence self-absorption during high concentration and fluorescence quenching manifests; thus the loss of the short-wavelength light (blue light) of fluorescence Spectra caused, cause the variation of fluorescence Spectra shape, promptly the 3DEEM of rhodamine B spectrum shape can be influenced by mass concentration "
If existing can absorb excites or fluorescent material is launched the material of luminous energy, will make fluorescent weakening in the solution, this phenomenon is called " interior filter action ".When solution concentration was big, a part of fluorescent emission was absorbed by self, produced so-called " self-absorption " phenomenon and the fluorescence intensity of solution that reduced.
Fluorescence quantum yield
Be defined as the ratio of photon number with the photon number of the exciting light that is absorbed of institute's emitted fluorescence behind the fluorescent material extinction.That is:
And the light intensity (Ia) that fluorescence intensity (If) should absorb with solution and the fluorescence quantum yield of this material
Relevant, i.e. fluorescence intensity I
fBe proportional to the light intensity Ia and the fluorescence quantum yield of absorption
T is a transmissivity, I
0Be incident intensity, It is a transmitted intensity;
Because: A=ε lc A=-lgT=lg1/T=lg I
0/ It T=10
-A=e
-2.3 ε lc
So:
ε is a molar absorptivity, and l is the light path of sample cell, and c is a sample concentration;
e
-2.3εlc＝1-2.3εlc-(2.3εlc)
2/2！-(2.3εlc)
3/3！-......
When ε lc≤0.05, every after can omitting second, that is: e
-2.3 ε lc=1-2.3 ε lc
As incident intensity I
0Certain with l, can be abbreviated as: I
f=Kc
Be that fluorescence intensity is directly proportional with the concentration of fluorescent material, but this linear relationship is only in extremely rare solution, when ε lc≤0.05, just sets up,,, make fluorescence intensity and concentration not linear owing to reasons such as quencher phenomenon and self-absorptions for denseer solution.As seen, different fluorescent materials has different fluorescent characteristicss and the concentration range of characteristic fluorescence occurs, but when concentration is high, interior optical filtering (inner filtering.) and self-absorption (reabsorbance) phenomenon will occur, and cause fluorescence intensity to reduce.Therefore when three-dimensional fluorescence spectrum detects, be necessary to carry out inner filtration and self-absorption and correct.
In the patented claim before the applicant (application number 200910157790.3), openly protect a kind of three-dimensional fluorescence spectrum antidote, the invention provides the fluorescence detector that conveniently to realize the three-dimensional fluorescence spectrum calibration function.This detector comes down to a kind ofly can detect light absorption (transmittance) and fluorophotometer.
Summary of the invention
The application provides a kind of fluorophotometer that can detect light absorption (transmittance), this equipment improves on original fluorophotometer basis, fluoroscopic examination and light absorption (transmittance) detected combine, reach can test sample fluorescence (comprising three-dimensional fluorescence, synchronous fluorescence) spectrum again can test sample to the purpose of the absorption spectrum (for example absorbance or transmittance etc.) of light.The application's fluorophotometer increases some accessories on original fluorophotometer basis, this fluorophotometer can be detected the transmitted light that sees through sample, and to reach the purpose of test sample to light absorption, this is innovative point of the present invention place.
In the present invention, exciting light is meant by light emitted, is used for the light of excited sample.Launch that sample is only excited and the light launched.Transmitted light is meant that exciting light passes behind the sample cell, along the light of exciting light direction transmission.Reflected light is meant the light that obtains after transmitted light is through the reflecting system reflection.
And the disclosed wherein a kind of scheme of the application is: on the basis of existing fluorophotometer, increase a transmitted light reflecting system, to enter detecting device by the transmitted light reflection of sample, and reach the purpose of test sample to the light absorption situation, it is shown in Figure 1 referring to Figure of description that it forms structure.And existing fluorophotometer generally includes light source, exciting light monochromator, sample cell, emission monochromator, parts such as detecting device.
Fluorophotometer described in the application's first scheme comprises light source, exciting light monochromator, sample cell, emission monochromator, detecting device and transmitted light reflecting system, wherein, the transmitted light reflecting system enters detecting device with the transmitted light reflection, can be used for detecting transmissivity.Further, this photometer also comprises emission slit (slit that emission light will pass through) and reflection slit (slit that reflected light will pass through).
Spectrophotometric sensing range is determined by the optical source wavelength scope of fluorophotometer, general wavelength is 200-900nm, this wavelength coverage be it is generally acknowledged and comprised the ultraviolet scope, therefore, the spectrophotometric sensing range of mentioning among the present invention also is the scope of normally used 200-900nm wavelength.
When tester's target is when obtaining fluorescence data, the slit that can set the reflecting system part is 0 (promptly closes slit, avoid transmitted light to see through), and the operation according to normal fluorophotometer obtains corresponding fluorescence data then.
When tester's target is when obtaining the ultraviolet spectroscopic data of sample, sample just is set, and to launch the emission slit that fluorescence will pass through be 0, only allow transmitted light enter detecting device, so that test sample is to optical transmission rate (absorbance log), spectroscopic data is absorbed.
When tester's target is during for the three-dimensional fluorescence testing result that obtains correcting, slit that need to set the reflecting system part is 0 (promptly to close slit, avoid transmitted light to see through), after measuring the three-dimensional fluorescence data, sample is set again, and to launch the emission slit that fluorescence will pass through be 0 (promptly close slit, avoid fluorescence to see through), pass through reflecting system, only allow transmitted light enter detecting device, just can test sample to optical transmission rate (absorbance), and data are passed to data processor.By data processor fluorescence spectrum is corrected, is obtained proofreading and correct the back fluorescence data, antidote be the fluorescence intensity of each emission wavelength of fluorescence spectrum divided by the sample of identical wavelength to optical transmission rate (I
f/ T).
This reflecting system can be any device that can realize transmitted light is reflexed to former detecting device.Can be the level crossing that simply has reflection function, spherical mirror, polygon prism etc.; Or can realize one group of catoptron (spherical reflector or plane mirror) of reflection function, or a kind of polygon prism group etc.
Described reflecting system specifically can be a plane mirror, spherical reflector, reflecting prism, a reflector group, a reflecting prism group, the perhaps combination of above-mentioned catoptron, and being provided with according to being provided with of concrete fluorophotometer of catoptron is definite.
The disclosed another kind of scheme of the application is, on existing fluorophotometer basis, increases another detecting device on the transmission direction of light, and test sample is to the absorption situation (transmissivity) of light.After transforming, this fluorophotometer can be realized the function of fluorescence and ultraviolet/visible spectrophotometer joint-detection, or has the fluorophotometer of correcting function.
Described fluorophotometer comprises light source, exciting light monochromator, sample cell, emission monochromator, detecting device A (detecting device of former fluorophotometer) and another detecting device B that is provided with in the transmitted light direction.Wherein, detecting device A is used for detecting emission light, be also referred to as the emission photodetector, and detecting device B is used for the detection of transmitted light, can be described as transmitted light detector.But the transmitted light scioptics enter detecting device B, and detecting device B is used for test sample to the optical transmission rate.
When tester's target is when obtaining fluorescence data,, obtain corresponding fluorescence data according to the operation of the fluorophotometer of routine with emission photodetector A.
When tester's target is when obtaining sample to the absorption of light or transmission data, then to allow the transmitted light scioptics enter transmitted light detector B, with detecting device B test sample to optical transmission rate (absorbance).
When tester's target is during for the three-dimensional fluorescence testing result that obtains correcting, detects with detecting device A according to the method for routine and to obtain the three-dimensional fluorescence data.Afterwards, allow the transmitted light scioptics enter detecting device B, use detecting device B test sample, and data are passed to data processor (data processor in the former fluorophotometer) optical transmission rate (absorbance).By data processor fluorescence spectrum is corrected, obtained proofreading and correct the back fluorescence data.Antidote be the fluorescence intensity of each emission wavelength correspondence of fluorescence spectrum divided by the sample of identical wavelength to optical transmission rate (I
f/ T).
Description of drawings
Fig. 1 band reflecting system can detect light absorption or light transmissive fluorophotometer.
Fig. 2 band dual-detector can detect light absorption or light transmissive fluorophotometer:
Embodiment
As the disclosed fluorophotometer of Fig. 1.This fluorophotometer comprises light source (being generally xenon lamp or the high-pressure sodium lamp of wavelength 200-900nm), exciting light monochromator, entrance slit, sample cell, emission slit, emission monochromator, detecting device, amplifier, registering instrument, reflection slit and transmitted light reflecting system.The transmitted light reflecting system enters detecting device with the transmitted light reflection, so that detect transmissivity.This transmitted light reflecting system may be simple catoptron, reflecting prism, also can be reflector group or reflecting prism group.Decide with being provided with according to concrete fluorophotometer setting the position of concrete transmitted light reflecting system, and the simplest mode is only to pass through reflector group, adjusts it and settle angle that transmitted light is reflected into into photodetector, reaches the purpose that detects light absorption (transmittance).
At the reflected light place reflection slit is set among Fig. 1, when the accommodation reflex slit is 0 (promptly closing slit), reflected light also will no longer enter detecting device.And close the emission slit, fluorophotometer can be become ultraviolet/visible spectrophotometer.
As the disclosed fluorophotometer of Fig. 2.This fluorophotometer comprises light source (being generally xenon lamp or the high-pressure sodium lamp of wavelength 200-900nm), exciting light monochromator, entrance slit, sample cell, diaphotoscope, emission slit, detecting device A, lens, detecting device B, amplifier, registering instrument.Wherein, detecting device B is positioned at the transmitted light direction, and directly test sample is to the optical transmission rate, and the transmitted light scioptics enter detecting device B.
For Fig. 1 and the described equipment of Fig. 2, the fluorescence intensity of each emission wavelength correspondence of fluorescence spectrum is just obtained correcting the back fluorescence spectrum divided by the transmissivity T of identical wavelength, realize rectification to fluorescence spectrum.
The disclosed fluorophotometer that can detect light absorption (transmittance) of the application can realize detecting simultaneously easily fluorescence spectrum and ultraviolet spectrum, also can obtain the three-dimensional fluorescence spectrum data of correcting through the ultraviolet transmissivity easily.
Claims (5)
1. one kind can be detected light absorption or light transmissive fluorophotometer, comprise light source, exciting light monochromator, sample cell, emission monochromator, detecting device, it is characterized in that, this fluorophotometer comprises a reflecting system, this reflecting system will be by the former fluorophotometer detecting device of the transmitted light reflected back of sample, and described detecting device test sample is to the optical transmission rate.
2. fluorophotometer according to claim 1, described reflecting system are a plane mirror, spherical reflector, reflecting prism, reflector group, a reflecting prism group, the perhaps combination of above-mentioned catoptron.
3. fluorophotometer as claimed in claim 1 or 2 further comprises the reflection slit, and described slit can open or close.
4. one kind can be detected light absorption or light transmissive fluorophotometer, comprise light source, exciting light monochromator, sample cell, emission monochromator, detecting device A, it is characterized in that, in this fluorophotometer, transmitted light direction at sample, also comprise another transmitted light detector B, detecting device B test sample is to the optical transmission rate.
5. fluorophotometer as claimed in claim 4 is characterized in that, enters transmitted light detector B behind the described transmitted light scioptics.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101706434B (en) * | 2009-09-04 | 2011-08-10 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN104111243A (en) * | 2014-07-11 | 2014-10-22 | 江苏大学 | Fluorescence ratio measuring system and method |
| CN104508440A (en) * | 2012-08-20 | 2015-04-08 | 株式会社日立高新技术 | Spectrophotometer |
| CN106323972A (en) * | 2016-10-26 | 2017-01-11 | 桂林理工大学 | Judging device for spectrophotometric titration end point |
| CN106323971A (en) * | 2016-10-26 | 2017-01-11 | 桂林理工大学 | Method for judging titration end point by utilizing absorbancy |
| CN106706574A (en) * | 2015-11-12 | 2017-05-24 | 中国科学院大连化学物理研究所 | Weak fluorescence detection device |
| CN107664620A (en) * | 2016-07-29 | 2018-02-06 | 上海溯源生物技术有限公司 | A kind of method of AAS or Fluorometric assay object |
| CN109387495A (en) * | 2018-10-09 | 2019-02-26 | 东北大学秦皇岛分校 | A kind of double light source photocatalytic reaction devices |
| CN112285124A (en) * | 2020-11-09 | 2021-01-29 | 南京财经大学 | Method and device for judging aflatoxin B1 pollution degree in vegetable oil and optical judging device |
-
2009
- 2009-09-04 CN CN2009201762454U patent/CN201689050U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101706434B (en) * | 2009-09-04 | 2011-08-10 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN104508440A (en) * | 2012-08-20 | 2015-04-08 | 株式会社日立高新技术 | Spectrophotometer |
| CN104111243A (en) * | 2014-07-11 | 2014-10-22 | 江苏大学 | Fluorescence ratio measuring system and method |
| CN104111243B (en) * | 2014-07-11 | 2017-01-04 | 江苏大学 | A kind of ratio fluorescent measures system and method |
| CN106706574A (en) * | 2015-11-12 | 2017-05-24 | 中国科学院大连化学物理研究所 | Weak fluorescence detection device |
| CN106706574B (en) * | 2015-11-12 | 2019-03-19 | 中国科学院大连化学物理研究所 | A kind of detection device of hypofluorescence |
| CN107664620A (en) * | 2016-07-29 | 2018-02-06 | 上海溯源生物技术有限公司 | A kind of method of AAS or Fluorometric assay object |
| CN106323972A (en) * | 2016-10-26 | 2017-01-11 | 桂林理工大学 | Judging device for spectrophotometric titration end point |
| CN106323971A (en) * | 2016-10-26 | 2017-01-11 | 桂林理工大学 | Method for judging titration end point by utilizing absorbancy |
| CN109387495A (en) * | 2018-10-09 | 2019-02-26 | 东北大学秦皇岛分校 | A kind of double light source photocatalytic reaction devices |
| CN112285124A (en) * | 2020-11-09 | 2021-01-29 | 南京财经大学 | Method and device for judging aflatoxin B1 pollution degree in vegetable oil and optical judging device |
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