CN112557358A - Online fluorescence detection light path of water pollutant - Google Patents
Online fluorescence detection light path of water pollutant Download PDFInfo
- Publication number
- CN112557358A CN112557358A CN202011339036.4A CN202011339036A CN112557358A CN 112557358 A CN112557358 A CN 112557358A CN 202011339036 A CN202011339036 A CN 202011339036A CN 112557358 A CN112557358 A CN 112557358A
- Authority
- CN
- China
- Prior art keywords
- reflector
- light
- flow cell
- path
- fluorescence detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
Abstract
The invention relates to an online fluorescence detection light path for water pollutants, which comprises a light source and a flow cell made of a light-transmitting material, wherein light of the light source can be transmitted in the flow cell, reflector groups are arranged on the periphery of the flow cell, each reflector group comprises a first reflector, a second reflector and a third reflector, the first reflector can receive the light transmitted through the flow cell and reflect the light to the second reflector, the second reflector can receive the light path reflected by the first reflector and reflect the light to the third reflector, the reflected light path between the second reflector and the third reflector is arranged through the flow cell, the third reflector can receive the light path reflected by the second reflector and reflect the light to the second reflector, and the light path passes through the flow cell for multiple times under the reflection of the reflector groups. By adopting the technical scheme, namely, the multi-path reflection type light path is adopted, the excitation superposition of the excitation light for four times is realized on the detection channel, and the spectral excitation efficiency of the target object to be detected is obviously improved.
Description
Technical Field
The invention belongs to the technical field of water pollutant detection, and particularly relates to an online fluorescence detection light path for water pollutants.
Background
In recent years, with the development of organic chemical industry, petrochemical industry and pharmaceutical industry, the pollution problem of water ecological environment is becoming more serious, and water pollution is showing the trend of regional expansion, diversified pollution and serious degree. On-line detection of water quality is becoming a main means for prevention and control of water pollution, and among them, fluorescence analysis is widely used due to its advantages such as high sensitivity and good selectivity.
However, the currently adopted fluorescence detection optical path for water pollutants usually has the problem of low excitation efficiency of a light source when detecting low-content target objects.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide an online fluorescence detection light path for water pollutants, which has the advantage of improving the spectral excitation efficiency.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides an online fluorescence detection light path of water pollutant, includes the light source and the flow-through cell of making by the printing opacity material, the light of light source can propagate in the flow-through cell, be provided with the speculum group around the flow-through cell, the speculum group is including first speculum, second speculum and third speculum, first speculum acceptable sees through the light of flow-through cell and reflect it to the second speculum, the second speculum can accept the light path that first speculum reflected and reflect to the third speculum, the second speculum with reflection light path between the third speculum sees through the flow-through cell setting, the third speculum can accept the light path that the second speculum reflected and reflect back the second speculum, the light path is in pass many times under the reflection of speculum group the flow-through cell.
By adopting the technical scheme, in the water sample detection process, exciting light emitted by the light source penetrates through the flow cell and then reaches the first reflector, the exciting light is reflected by the first reflector and then reaches the second reflector positioned on the vertical light path, the exciting light is reflected by the third reflector and then reaches the third reflector through the flow cell, the second reflector and the first reflector, the exciting light penetrates through the flow cell for four times under the action of the three groups of reflectors in the exciting light path, namely, a multipath reflection-type light path is adopted, the excitation superposition of the exciting light for four times on the detection channel is realized, and the spectral excitation efficiency of the target object to be detected is obviously improved.
The invention is further configured to: one end of the flow cell is provided with a detection optical filter, the outer end of the detection optical filter is provided with a first detector, and the other end of the flow cell is provided with a plane reflector for enhancing optical detection signals.
By adopting the technical scheme, the other end of the flow cell is provided with the plane reflector for enhancing the optical detection signal, and the fluorescence signal of the detection light path is improved in a fluorescence reflection mode; meanwhile, one end of the flow cell is provided with a detection optical filter, the detection optical filter filters a light path, only light with specific wavelength is allowed to pass through for detection, and background interference of detection is reduced.
The invention is further configured to: the bottom surface of flow-through cell is provided with the introduction port, the top surface of flow-through cell is kept away from introduction port one end is provided with out the appearance mouth.
Through adopting above-mentioned technical scheme, the sample inlet one end is kept away from to the top surface of flow-through cell is provided with the outlet, adopts the sample detection pond of circulation formula for the device is applicable in the on-line measuring of all kinds of water target pollutants that have fluorescence signal.
The invention is further configured to: the light source with set gradually first light filter and spectroscope between the flow cell, be 45 contained angles between spectroscope and the horizontal optical axis, the spectroscope is used for with the light branch of light source is light source reference and exciting light, the below of spectroscope is provided with and is used for detecting the second detector of light source reference.
By adopting the technical scheme, the online fluorescence detection technology adopts a multi-path reflection type light path, 4 times of excitation superposition of excitation light is realized on the detection channel, and the spectral excitation efficiency of the target object to be detected is obviously improved.
The invention is further configured to: and a condenser lens for converging the exciting light is arranged between the spectroscope and the flow cell.
Through adopting above-mentioned technical scheme, be provided with the condensing lens between spectroscope and the flow cell, focus the light path of exciting light to the flow cell for the exciting light assembles in the flow cell and shines on the sample of flow cell, has improved exciting light intensity, has promoted the sensitivity that detects.
The invention is further configured to: the first reflector is located the flow-through cell is kept away from one side of light source, the second reflector is located the below of flow-through cell, first reflector and horizontal optical axis the contained angle that is the same setting between second reflector and the perpendicular optical axis, the third reflector is located the top of flow-through cell, parallel arrangement between third reflector and the horizontal optical axis.
By adopting the technical scheme, included angles formed by the first reflector and the horizontal optical axis and included angles formed by the second reflector and the vertical optical axis are the same, so that light can be repeatedly reflected and transmitted in the reflector group, a multi-path reflection type optical path is realized, excitation superposition of excitation light for four times is realized on the detection channel, and the spectrum excitation efficiency of the target object to be detected is obviously improved.
The invention is further configured to: the first reflecting mirror, the second reflecting mirror and the third reflecting mirror are all concave mirrors.
Through adopting above-mentioned technical scheme, first speculum, second speculum and third speculum three are the concave mirror, and the concave mirror is better to the focusing effect of light path.
The invention is further configured to: the first reflector, the second reflector and the third reflector are plane mirrors.
Through adopting above-mentioned technical scheme, first speculum, second speculum and third speculum three are the level crossing, and the level crossing cost is lower, has reduced the detection cost.
The invention is further configured to: the first detector is a photomultiplier tube and the second detector is a silicon photodiode.
By adopting the technical scheme, the silicon photodiode has good linearity and low cost, is suitable for being used for detecting light source reference, and reduces the manufacturing cost of the device; meanwhile, when the first reflecting mirror, the second reflecting mirror and the third reflecting mirror are all concave mirrors, the photomultiplier has high sensitivity and good signal-to-noise ratio, is suitable for detecting exciting light, and reduces background interference.
The invention is further configured to: the first detector and the second detector are both silicon photodiodes.
By adopting the technical scheme, the parallel light path is formed in the plane mirror, the light path does not need to be focused, the silicon photodiode is good in linearity, and the detection cost of the device is reduced.
In conclusion, the invention has the following beneficial effects:
1. the exciting light passes through the flow cell for four times under the action of the three groups of reflectors in the excitation light path, namely, a multi-path reflection light path is adopted, so that the excitation and superposition of the exciting light for four times are realized on the detection channel, and the spectral excitation efficiency of the target object to be detected is obviously improved;
2. a plane reflector for enhancing optical detection signals is arranged at the other end of the flow cell, and fluorescence signals of a detection light path are improved in a fluorescence reflection mode; meanwhile, one end of the flow cell is provided with a detection optical filter, the detection optical filter filters a light path, only light with specific wavelength is allowed to pass through for detection, and background interference of detection is reduced.
Drawings
FIG. 1 is a diagram of a detection optical path according to the first embodiment;
FIG. 2 is a schematic structural view of the flow cell of the first and second embodiments;
fig. 3 is a diagram of a detection optical path of the second embodiment.
Reference numerals: 1. a light source; 2. a first optical filter; 3. a beam splitter; 4. a condenser lens; 5. a third reflector; 6. a flow-through cell; 61. a first detector; 62. a sample outlet; 63. a sample inlet; 64. detecting the optical filter; 65. a plane mirror; 7. a first reflector; 8. a second reflector; 9. a second detector.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example one
As shown in fig. 1 to 2, the online fluorescence detection optical path for water pollutants disclosed by the present invention includes a light source 1 and a flow cell 6 made of a light-transmitting material, wherein the flow cell 6 is in the shape of a prism-shaped flow cell 6 with four sides transmitting light and two ends being sealed, the light source 1 can be one of an LED, a xenon lamp, a tungsten lamp, a deuterium lamp or a laser diode, and light of the light source 1 can be transmitted in the flow cell 6.
The speculum group is installed around the flow-through cell 6, the speculum group is including first speculum 7, second speculum 8 and third speculum 5, first speculum 7 can accept the light that sees through flow-through cell 6 and reflect it to second speculum 8, the light path that second speculum 8 can accept reflection of first speculum 7 reflects and reflects to third speculum 5, the reflection light path between second speculum 8 and the third speculum 5 sees through flow-through cell 6 and sets up, the light path that third speculum 5 can accept the reflection of second speculum 8 and reflects back to second speculum 8, the light path passes flow-through cell 6 many times under the reflection of speculum group.
A first optical filter 2 and a spectroscope 3 are sequentially arranged and installed between a light source 1 and a flow cell 6, an included angle of 45 degrees is formed between the spectroscope 3 and a horizontal optical axis, the spectroscope 3 is used for dividing light of the light source 1 into reference light of the light source 1 and exciting light, and a second detector 9 used for detecting reference light of the light source 1 is installed below the spectroscope 3.
One end of the flow cell 6 is covered with a detection filter 64, the outer end of the detection filter 64 is fixedly connected with a first detector 61, and the other end of the flow cell 6 is fixedly connected with a plane mirror 65 for enhancing the optical detection signal. The first detector 61 and the second detector 9 are both silicon photodiodes.
The bottom surface of the flow cell 6 is provided with a sample inlet 63, and one end of the top surface of the flow cell 6, which is far away from the sample inlet 63, is provided with a sample outlet 62.
The working conditions and principles of the embodiment are as follows:
in the water sample detection process, excitation light emitted by the light source 1 is divided into reference light source 1 and excitation light after passing through the first optical filter 2 and the spectroscope 3, and the reference light source 1 is received by the second detector 9 for detection.
The exciting light is converged by the condenser 4, then penetrates through the flow cell 6, then reaches the first reflector 7, is reflected by the first reflector, then reaches the second reflector 8 positioned on the vertical light path, is reflected by the second reflector, then penetrates through the flow cell 6, and finally reaches the third reflector 5, and the exciting light reflected by the third reflector 5 sequentially passes through the flow cell 6, the second reflector 8, the first reflector 7 and the flow cell 6. The excitation light is transmitted through the detection flow cell 6 four times in the excitation light path under the action of the three groups of reflectors.
During the detection process, a water sample to be detected enters from the sample inlet 63 and flows out from the sample outlet 62, and a fluorescence signal of the sample is received by the first detector 61 through the flow cell 6.
The fluorescence detection optical path is used for online detection of oil pollutants in water, and detection results are collected and recorded in real time through the first detector 61 and are subjected to data correction with reference signals of the light source 1 collected by the second detector 9.
Example two
The difference between this embodiment and the first embodiment is: as shown in fig. 2 and 3, the first detector 61 is a photomultiplier tube, and the second detector 9 is a silicon photodiode.
The first reflecting mirror 7, the second reflecting mirror 8 and the third reflecting mirror 5 are all concave mirrors, a collecting mirror 4 for converging exciting light is arranged between the spectroscope 3 and the flow cell 6, and the collecting mirror 4 is a convex lens.
The working conditions and principles of the embodiment are as follows:
in the water sample detection process, excitation light emitted by the light source 1 is divided into reference light source 1 and excitation light after passing through the first optical filter 2 and the spectroscope 3, and the reference light source 1 is received by the second detector 9 for detection.
The exciting light penetrates through the flow cell 6 and then reaches the first reflecting mirror 7, and then reaches the second reflecting mirror 8 positioned on the vertical light path after being reflected by the first reflecting mirror 7, and then reaches the third reflecting mirror 5 after being reflected by the second reflecting mirror 6, and the exciting light reflected by the third reflecting mirror 5 passes through the flow cell 6, the second reflecting mirror 8, the first reflecting mirror 7 and the flow cell 6 in sequence. The excitation light is transmitted through the detection flow cell 6 four times in the excitation light path under the action of the three groups of reflectors.
During the detection process, a water sample to be detected enters from the sample inlet 63 and flows out from the sample outlet 62, and a fluorescence signal of the sample is received by the first detector 61 through the flow cell 6.
The fluorescence detection optical path is used for online detection of oil pollutants in water, and detection results are collected and recorded in real time through the first detector 61 and are subjected to data correction with reference signals of the light source 1 collected by the second detector 9.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.
Claims (10)
1. An online fluorescence detection light path for water pollutants is characterized in that: the LED light source comprises a light source (1) and a flow cell (6) made of light-transmitting materials, light of the light source (1) can be transmitted in the flow cell (6), reflector groups are arranged on the periphery of the flow cell (6) and comprise a first reflector (7), a second reflector (8) and a third reflector (5), the first reflector (7) can receive light penetrating through the flow cell (6) and reflect the light to the second reflector (8), the second reflector (8) can receive a light path reflected by the first reflector (7) and reflect the light to the third reflector (5), a reflection light path between the second reflector (8) and the third reflector (5) penetrates through the flow cell (6), and the third reflector (5) can receive the light path reflected by the second reflector (8) and reflect the light to the second reflector (8), the light path passes through the flow cell (6) multiple times under the reflection of the reflector group.
2. The on-line fluorescence detection path for water contaminants of claim 1, wherein: one end of the flow cell (6) is provided with a detection filter (64), the outer end of the detection filter (64) is provided with a first detector (61), and the other end of the flow cell (6) is provided with a plane reflector (65) for enhancing optical detection signals.
3. The on-line fluorescence detection path for water contaminants of claim 2, wherein: the bottom surface of flow-through cell (6) is provided with introduction port (63), the top surface of flow-through cell (6) is kept away from introduction port (63) one end is provided with out appearance mouth (62).
4. The on-line fluorescence detection path for water contaminants of claim 2, wherein: light source (1) with set gradually first light filter (2) and spectroscope (3) between flow cell (6), be 45 contained angles between spectroscope (3) and the horizontal optical axis, spectroscope (3) are used for with the light branch of light source (1) is for light source (1) reference and exciting light, the below of spectroscope (3) is provided with and is used for detecting second detector (9) of light source (1) reference.
5. The on-line fluorescence detection path for water contaminants of claim 4, wherein: and a condenser (4) for converging the exciting light is arranged between the spectroscope (3) and the flow cell (6).
6. The on-line fluorescence detection path for water contaminants of claim 1, wherein: first speculum (7) are located flow cell (6) are kept away from one side of light source (1), second mirror (8) are located the below of flow cell (6), first speculum (7) and horizontal optical axis the contained angle that is the same setting between second mirror (8) and the perpendicular optical axis, third speculum (5) are located the top of flow cell (6), parallel arrangement between third speculum (5) and the horizontal optical axis.
7. The on-line fluorescence detection path for water contaminants of claim 5, wherein: the first reflecting mirror (7), the second reflecting mirror (8) and the third reflecting mirror (5) are all concave mirrors.
8. The on-line fluorescence detection path for water contaminants of claim 4, wherein: the first reflector (7), the second reflector (8) and the third reflector (5) are plane mirrors.
9. The on-line fluorescence detection path for water contaminants of claim 7, wherein: the first detector (61) is a photomultiplier and the second detector (9) is a silicon photodiode.
10. The on-line fluorescence detection path for water contaminants of claim 8, wherein: the first detector (61) and the second detector (9) are both silicon photodiodes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011339036.4A CN112557358A (en) | 2020-11-25 | 2020-11-25 | Online fluorescence detection light path of water pollutant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011339036.4A CN112557358A (en) | 2020-11-25 | 2020-11-25 | Online fluorescence detection light path of water pollutant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112557358A true CN112557358A (en) | 2021-03-26 |
Family
ID=75043634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011339036.4A Pending CN112557358A (en) | 2020-11-25 | 2020-11-25 | Online fluorescence detection light path of water pollutant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112557358A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000046731A (en) * | 1998-07-28 | 2000-02-18 | Matsushita Electric Ind Co Ltd | Angle-of-rotation measuring device |
| CN101514920A (en) * | 2008-02-20 | 2009-08-26 | 厦门大学 | Ultraviolet spectrophotometer, detecting head |
| CN202230024U (en) * | 2011-08-24 | 2012-05-23 | 中国计量学院 | Fluorescence enhancement type optical fiber fluorescent probe |
| CN104597026A (en) * | 2014-12-31 | 2015-05-06 | 苏州优谱德精密仪器科技有限公司 | Device for enhancing raman spectrum |
| CN106124404A (en) * | 2016-07-21 | 2016-11-16 | 中国科学院合肥物质科学研究院 | A kind of multiple reflections absorbs sample cell |
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
| CN110596005A (en) * | 2019-09-25 | 2019-12-20 | 安徽理工大学 | Novel annular flat concave mirror optical multi-pass absorption pool |
-
2020
- 2020-11-25 CN CN202011339036.4A patent/CN112557358A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000046731A (en) * | 1998-07-28 | 2000-02-18 | Matsushita Electric Ind Co Ltd | Angle-of-rotation measuring device |
| CN101514920A (en) * | 2008-02-20 | 2009-08-26 | 厦门大学 | Ultraviolet spectrophotometer, detecting head |
| CN202230024U (en) * | 2011-08-24 | 2012-05-23 | 中国计量学院 | Fluorescence enhancement type optical fiber fluorescent probe |
| CN104597026A (en) * | 2014-12-31 | 2015-05-06 | 苏州优谱德精密仪器科技有限公司 | Device for enhancing raman spectrum |
| CN106124404A (en) * | 2016-07-21 | 2016-11-16 | 中国科学院合肥物质科学研究院 | A kind of multiple reflections absorbs sample cell |
| CN110441278A (en) * | 2019-08-29 | 2019-11-12 | 北京卓立汉光仪器有限公司 | A kind of device and method making signal multiplication |
| CN110596005A (en) * | 2019-09-25 | 2019-12-20 | 安徽理工大学 | Novel annular flat concave mirror optical multi-pass absorption pool |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3066452B1 (en) | Improvements relating to particle characterisation | |
| US4501970A (en) | Fluorometer | |
| JP4259762B2 (en) | Optical configuration for detecting light | |
| CN104614362A (en) | Free space gas Raman scattering collecting device | |
| CN112414992A (en) | Raman spectrum excitation enhancement module | |
| CN111175425A (en) | Diode array detector based on multistage wavelength calibration and calibration method | |
| CN103196879A (en) | Laser-induced fluorescence spectrum detection device | |
| CN201289459Y (en) | Collecting device for enhancing gas absorption optical distance | |
| GB2125181A (en) | Flow cells for particle study | |
| CN109799204B (en) | Low concentration COD measuring device based on spectrum method | |
| CN106066320B (en) | Seawater bacteria detection system based on multi-wavelength laser induced bacteria intrinsic fluorescence | |
| CN207946357U (en) | A kind of fluorescence excitation device of fluorescence detector | |
| CN112557358A (en) | Online fluorescence detection light path of water pollutant | |
| CN205484055U (en) | Fluorescence detection system and multichannel detection device | |
| CN208766107U (en) | A kind of phosphor collection device of fluorescence detector | |
| CN204694628U (en) | A kind of proportional double light beam spectrophotometric meter device | |
| CN212059104U (en) | Wide-spectrum high-sensitivity Raman spectrometer | |
| CN115096795A (en) | Flow type fluorescence detection optical system | |
| CN212008328U (en) | ICP-AES optical path system | |
| CN2589969Y (en) | Sulfuretted hydrogen gas online monitor | |
| CN202256141U (en) | Double-optical path high-sensitivity color comparison device | |
| CN111521569A (en) | Multi-light-source rotary colorimetric tube colorimetric device and determination method thereof | |
| CN219200630U (en) | Off-axis optical path system of ultraviolet detector | |
| CN102359940B (en) | Double-optical path and high-sensitivity colorimetric device and method thereof for detecting substance concentration | |
| CN212722627U (en) | Test paper strip color development detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 510070 Guangzhou City, Guangzhou, Guangdong, No. 34 Applicant after: Institute of testing and analysis, Guangdong Academy of Sciences (Guangzhou analysis and testing center, China) Address before: 510070 Guangzhou City, Guangzhou, Guangdong, No. 34 Applicant before: GUANGDONG INSTITUTE OF ANALYSIS (CHINA NATIONAL ANALYTICAL CENTER, GUANGZHOU) |
|
| CB02 | Change of applicant information |