CN205038162U - Fluorescence detection device - Google Patents
Fluorescence detection device Download PDFInfo
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- CN205038162U CN205038162U CN201520835340.6U CN201520835340U CN205038162U CN 205038162 U CN205038162 U CN 205038162U CN 201520835340 U CN201520835340 U CN 201520835340U CN 205038162 U CN205038162 U CN 205038162U
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 36
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- 238000001514 detection method Methods 0.000 claims abstract description 43
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- 238000005286 illumination Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
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- 238000005859 coupling reaction Methods 0.000 abstract description 5
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- 239000011162 core material Substances 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
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- 239000013308 plastic optical fiber Substances 0.000 description 1
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Abstract
The utility model discloses a fluorescence detection device, include the light source, excite optic fibre, light -splitting device, detection optic fibre, launching fiber and photoelectric detector, excite the one end of optic fibre to connect the light source, the other end that excites optic fibre is connected to the first end of light -splitting device, the one end of surveying optic fibre is connected to the second end of light -splitting device, and the other end of surveying optic fibre is used for connecting need checking sample, photoelectric detector is connected to launching fiber's one end, and the other end is connected to the third end of light -splitting device, the light source produces the exciting light, through exciting optical fiber transmission to light -splitting device, gets into after the light -splitting device beam split and surveys optic fibre, and detection optic fibre shines the exciting light on the need checking sample, after optic fibre is advanced to survey in optical coupling that need checking sample returned, get into launching fiber through the light -splitting device again, the light struck of launching fiber transmission is to the photoelectric detector, and photoelectric detector detects the light signal that launching fiber sent, the the fluorescence signal's that realizes sending the sample detection. The utility model has the characteristics of it is small, stable good.
Description
Technical field
The utility model belongs to fluoroscopic examination field, more specifically, relates to a kind of fluorescence detection device.
Background technology
Fluoroscopic examination is widely used in the fields such as material detection, life science, medical research.Fluorescence detection device comprises light source, monochromator or optical filter and photo-detector.The exciting light that light source sends is radiated at after on testing sample, and the light that sample returns incides on photo-detector after monochromator or optical filter, can qualitative or quantitative detecting the fluorescence signal of sample by the measured value detecting photo-detector.Its exciting light of conventional fluorescent pick-up unit and utilizing emitted light are propagated all in free space, and need the optical element for free space to carry out the structure of light path, its volume is larger.In free space light path, once the locus of certain optical element changes, all can there is deviation in its subsequent optical path, cause the decline of effect, and the usual volume of Free Space Optics part is also difficult to accomplish very high mechanical stability comparatively greatly.When detecting for fluorescence signal in the body of living body biological, the volume of the interior pry head of conventional fluorescent pick-up unit is comparatively large, is difficult to accomplish Wicresoft.
Utility model content
For the defect of prior art, the purpose of this utility model is to provide a kind of fluorescence detection device, is intended to solve fluorescence detection device of the prior art and causes owing to needing the optical element for free space to carry out light path structure the problem that volume is large, light stability is poor.
The utility model provides a kind of fluorescence detection device, comprises light source, excitation fiber, light-splitting device, detection optical fiber, launching fiber and photodetector; One end of described excitation fiber connects described light source, and the other end of described excitation fiber is connected to the first end of described light-splitting device; One end of described detection optical fiber is connected to the second end of described light-splitting device, and the other end of described detection optical fiber is for connecting testing sample; One end of described launching fiber connects described photodetector, and the other end is connected to the 3rd end of described light-splitting device; Described light source produces exciting light, and be transferred to described light-splitting device through described excitation fiber, after light-splitting device light splitting, enter detection optical fiber, exciting light is irradiated on testing sample by detection optical fiber; After detection optical fiber is entered in the optically-coupled that testing sample returns, launching fiber is entered again through light-splitting device, the illumination of launching fiber transmission is mapped on photodetector, and photodetector detects the light signal that launching fiber sends, and realizes the detection to the fluorescence signal that sample sends.
Further, described light source is fiber coupled laser, and described photodetector is photomultiplier.
Further, described fluorescence detection device also comprises the first optical fiber collimator between one end and described photomultiplier and the first optical filter that are connected to described launching fiber in turn; The optical alignment that described first optical fiber collimator is used for described launching fiber to transmit is to free space; Described first optical filter is used for carrying out filtering process to the light of free space.
Further, described light-splitting device comprises the second optical fiber collimator, the first fiber coupler, dichroic sheet and the second fiber coupler; Described second optical fiber collimator is arranged in the input path of described dichroic sheet, and the input end of described second optical fiber collimator is as the first end of described light-splitting device, the light that the output terminal of described second optical fiber collimator exports is as the incident light of described dichroic sheet; Described first fiber coupler is arranged on the reflected light path of described dichroic sheet, and one end of described first fiber coupler is as the second end of described light-splitting device, and the other end of described first fiber coupler is for receiving the light through described dichroic sheet reflection; Described second fiber coupler is arranged on the transmitted light path of described dichroic sheet, and one end of described second fiber coupler is as the 3rd end of described light-splitting device, and the other end of described second fiber coupler is for receiving the light through the transmission of described dichroic sheet.
Further, described light-splitting device also comprises the second optical filter be arranged between described second optical fiber collimator and described dichroic sheet.
Further, described light-splitting device comprises the 3rd optical filter also comprising and being arranged between described dichroic sheet and described second fiber coupler.
The utility model additionally provides a kind of fluorescence detection device, comprises fiber coupled laser, excitation fiber, the second optical fiber collimator, the first fiber coupler, dichroic sheet, the 3rd optical filter, detection optical fiber and photomultiplier; Described second optical fiber collimator is arranged in the input path of described dichroic sheet, and the input end of described second optical fiber collimator connects described fiber coupled laser by described excitation fiber; Described first fiber coupler is arranged on the reflected light path of described dichroic sheet, and one end of described first fiber coupler connects testing sample by described detection optical fiber; Described 3rd optical filter and described photomultiplier are successively set on the transmitted light path of described dichroic sheet.
The utility model utilizes the beam splitter in the optical fibre light splitting element replacement free space of Highgrade integration, the luminous energy transmitted in free space and light signal is limited in the optical device of optical fiber and Highgrade integration in conventional fluorescent pick-up unit; Make the volume of whole fluorescence detection device little and be not afraid of bending and vibration, achieving miniaturization and the high stability of fluorescence detection device.
Accompanying drawing explanation
Fig. 1 is the structural representation of the fluorescence detection device that the utility model embodiment provides.
Fig. 2 is the structural representation of the fluorescence detection device that the utility model first embodiment provides.
Fig. 3 is the structural representation of the fluorescence detection device that the utility model second embodiment provides.
Fig. 4 is the structural representation of the fluorescence detection device that the utility model the 3rd embodiment provides.
Fig. 5 is the structural representation of the fluorescence detection device that the utility model the 4th embodiment provides.
Fig. 6 is the structural representation of the fluorescence detection device that the utility model the 5th embodiment provides.
1 is light source, and 2 is excitation fiber, and 3 is light-splitting device, and 4 is detection optical fiber, 5 is testing sample, and 6 is launching fiber, and 7 is photodetector, and 8 is fiber coupled laser, 9 is y-type optical fiber, and 10 is the first optical fiber collimator, and 11 is the first optical filter, 12 is photomultiplier, and 13 is the second optical fiber collimator, and 14 is filter set shell, 15 is the first fiber coupler, and 16 is dichroic sheet, and 17 is the second fiber coupler, 18 is coupling fiber LED, and 19 is the second optical filter, and 20 is the 3rd optical filter.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
The fluorescence detection device that the utility model embodiment provides utilizes optical fiber to carry out luminous energy and optical signal transmission.Fig. 1 shows the structure of this fluorescence detection device, and for convenience of explanation, illustrate only the part relevant to the utility model embodiment, details are as follows:
Fluorescence detection device comprises light source 1, optical fiber and photodetector 7; Wherein light source 1 is for generation of exciting light, and optical fiber is used for the exciting light of transmission light source 1 generation and the fluorescence of testing sample transmitting, and photodetector 7 detects for the fluorescence launched testing sample 5.
The utility model utilizes the beam splitter in the optical fibre light splitting element replacement free space of Highgrade integration, the luminous energy transmitted in free space and light signal is limited in the optical device of optical fiber and Highgrade integration in conventional fluorescent pick-up unit.Optical fiber, as a kind of optical waveguide components of softness, has volume little and the feature of not being afraid of bending and vibrating, is beneficial to the miniaturization and high stability that realize fluorescence detection device.
Light source can be the light source that light emitting diode (LED), semiconductor laser, solid state laser, fiber laser or other forms of laser instrument etc. can realize coupling fiber.
Optical fiber can be single-mode fiber or multimode optical fiber, also can be the optical fiber of glass optical fiber, silica fibre, plastic optical fiber or other core materials.
Photodetector can be photodiode, temperature-sensitive power meter, pyroelectricity meter, avalanche diode, photomultiplier or other photodetectors.
The exciting light that light source 1 produces is coupled in excitation fiber 2 by optical fiber coupling device, and the light in excitation fiber 2 enters detection optical fiber 4 through light-splitting device 3, and exciting light is irradiated on testing sample 5 by detection optical fiber 4.After detection optical fiber 4 is entered in the optically-coupled that testing sample 5 returns, then enter launching fiber 6 through light-splitting device 3, the illumination of launching fiber 6 is mapped on photodetector 7.Photodetector 7 detects the light signal that launching fiber sends, and realizes the detection to the fluorescence signal that sample sends.
In order to the fluorescence detection device that further description the utility model provides, existing details are as follows with instantiation by reference to the accompanying drawings:
The structure of the fluorescence detection device that the utility model first embodiment provides as shown in Figure 2, is made up of fiber coupled laser 8, y-type optical fiber 9, first optical fiber collimator 10, first optical filter 11, photomultiplier 12.Y-type optical fiber 9 comprises and being made up of four parts, comprises excitation fiber 2, optical splitter 13, detection optical fiber 4 and launching fiber 6, and wherein excitation fiber 2, detection optical fiber 4 and launching fiber 6 are respectively three ends of y-type optical fiber 9.Excitation fiber 2 is entered in the optically-coupled that described fiber coupled laser 8 sends, then enters into detection optical fiber 4 through optical splitter 13, is then irradiated on sample 5.The light that sample 5 sends is coupled into detection optical fiber 4 again, enters launching fiber 6 through optical splitter 13, then is collimated to free space through the first optical fiber collimator 10, is irradiated on photomultiplier 12 after the first optical filter 11.
Y-type optical fiber 8 is a kind of optical fibre device having three interfaces.Y-type optical fiber 8 described in the utility model, its three interfaces can be divided into two groups, and one of them interface is one group, and another two interfaces are in another group.Suppose that the interface index of y-type optical fiber 8 is respectively A, B and C, wherein A is one group, B and C is another group.Y-type optical fiber 8 described in the utility model has following function: can export from C port from the light of A port input, can export from the light of C port input from B port.
First embodiment is simple structure of the present utility model, compact conformation, and stability is high, and extendability is strong.
The structure of the fluorescence detection device that the utility model second embodiment provides as shown in Figure 3, by fiber coupled laser 8, excitation fiber 2, second optical fiber collimator 13, filter set shell 14, dichroic sheet 16, first fiber coupler 15, detection optical fiber 4, launches the first optical filter 11 and photomultiplier 12 forms.The optically-coupled that described fiber coupled laser 8 exports enters in excitation fiber 2, then free space is collimated to through the second optical fiber collimator 13, enter into the first fiber coupler 15 through dichroic sheet 16 reflection, then be coupled into detection optical fiber 4 through the first fiber coupler 15, be irradiated on sample 5.The light that sample 5 sends is coupled into detection optical fiber 4 again, is oppositely collimated to free space through the first fiber coupler 15, is irradiated on photomultiplier 12 through dichroic sheet 16 and transmitting the first optical filter 11.
Second embodiment uses dichroic sheet as light-splitting device, and spectroscopical effeciency is higher, but structure is comparatively complicated.
The structure of the fluorescence detection device that the utility model the 3rd embodiment provides as shown in Figure 4, by fiber coupled laser 8, excitation fiber 2, second optical fiber collimator 13, filter set shell 14, dichroic sheet 16, first fiber coupler 15, second fiber coupler 17, detection optical fiber 4, is launched the first optical filter 11, launching fiber 6 and photomultiplier 12 and is formed.The optically-coupled that described fiber coupled laser 8 exports enters in excitation fiber 2, then free space is collimated to through the second optical fiber collimator 13, enter into the first fiber coupler 15 through dichroic sheet 16 reflection, then be coupled into detection optical fiber 4 through the first fiber coupler 15, be irradiated on sample 5.The light that sample 5 sends is coupled into detection optical fiber 4 again, oppositely free space is collimated to through the first fiber coupler 15, be irradiated on the second fiber coupler 17 through dichroic sheet 16 and transmitting the first optical filter 11, then be coupled into launching fiber 6, be then irradiated on photomultiplier 12.
3rd embodiment introduces launching fiber on the basis of the second embodiment, the beam-splitting structure comprising dichroic sheet can be made more compact, higher than the second embodiment in miniaturization and stability.
The structure of the fluorescence detection device that the utility model the 4th embodiment provides as shown in Figure 5, is made up of fiber coupled laser 8, excitation fiber 2, second optical fiber collimator 13, first optical fiber collimator 10, filter set shell 14, dichroic sheet 16, first fiber coupler 15, second fiber coupler 17, detection optical fiber 4, launching fiber 6, first optical filter 11 and photomultiplier 12.The optically-coupled that fiber coupled laser 8 exports enters in excitation fiber 2, then free space is collimated to through the second optical fiber collimator 13, enter into the first fiber coupler 15 through dichroic sheet 16 reflection, then be coupled into detection optical fiber 4 through the first fiber coupler 15, be irradiated on sample 5.The light that sample 5 sends is coupled into detection optical fiber 4 again, oppositely free space is collimated to through the first fiber coupler 15, be irradiated on the second fiber coupler 17 through dichroic sheet 16, be coupled into launching fiber 6 again, then be collimated to free space through the first optical fiber collimator 10, be irradiated on photomultiplier 12 after launching the first optical filter 11.
4th embodiment introduces transmitting optical filter on the basis of the 3rd embodiment, can improve signal to noise ratio (S/N ratio).
The structure of the fluorescence detection device that the utility model the 5th embodiment provides as shown in Figure 6, by coupling fiber LED18, excitation fiber 2, second optical fiber collimator 13, first optical fiber collimator 10, filter set shell 14, second optical filter 19, dichroic sheet 16, first fiber coupler 15, second fiber coupler 17, detection optical fiber 4, launching fiber 6, launches the first optical filter 11 and photomultiplier 12 forms.The optically-coupled that described fiber coupled laser 8 exports enters in excitation fiber 2, then free space is collimated to through the second optical fiber collimator 13, through the second optical filter 19, the first fiber coupler 15 is entered into through dichroic sheet 16 reflection, be coupled into detection optical fiber 4 through the first fiber coupler 15 again, be irradiated on sample 5.The light that sample 5 sends is coupled into detection optical fiber 4 again, oppositely free space is collimated to through the first fiber coupler 15, be irradiated on the second fiber coupler 17 through dichroic sheet 16, be coupled into launching fiber 6 again, then be collimated to free space through the first optical fiber collimator 10, be irradiated on photomultiplier 12 after launching the first optical filter 11.
5th embodiment introduces exciter filter on the basis of the 4th embodiment, can stray light in filtering wideband light source, can improve signal to noise ratio (S/N ratio) using the occasion (such as using LED illumination) of wideband light source.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (7)
1. a fluorescence detection device, it is characterized in that, comprise light source (1), excitation fiber (2), light-splitting device (3), detection optical fiber (4), launching fiber (6) and photodetector (7);
One end of described excitation fiber (2) connects described light source (1), and the other end of described excitation fiber (2) is connected to the first end of described light-splitting device (3);
One end of described detection optical fiber (4) is connected to the second end of described light-splitting device (3), and the other end of described detection optical fiber (4) is for connecting testing sample (5);
One end of described launching fiber (6) connects described photodetector (7), and the other end is connected to the 3rd end of described light-splitting device (3);
Described light source (1) produces exciting light, described light-splitting device (3) is transferred to through described excitation fiber (2), after light-splitting device (3) light splitting, enter detection optical fiber (4), exciting light is irradiated on testing sample (5) by detection optical fiber (4); After detection optical fiber (4) is entered in the optically-coupled that testing sample (5) returns, launching fiber (6) is entered again through light-splitting device (3), the illumination that launching fiber (6) transmits is mapped on photodetector (7), photodetector (7) detects the light signal that launching fiber sends, and realizes the detection to the fluorescence signal that sample sends.
2. fluorescence detection device as claimed in claim 1, it is characterized in that, described light source (1) is fiber coupled laser (8), and described photodetector (7) is photomultiplier (12).
3. fluorescence detection device as claimed in claim 2, it is characterized in that, described fluorescence detection device also comprises the first optical fiber collimator (10) between one end and described photomultiplier (12) and the first optical filter (11) that are connected to described launching fiber (6) in turn;
Described first optical fiber collimator (10) for optical alignment that described launching fiber (6) is transmitted to free space; Described first optical filter (11) is for carrying out filtering process to the light of free space.
4. fluorescence detection device as claimed in claim 3, it is characterized in that, described light-splitting device (3) comprises the second optical fiber collimator (13), the first fiber coupler (15), dichroic sheet (16) and the second fiber coupler (17);
Described second optical fiber collimator (13) is arranged in the input path of described dichroic sheet (16), and the input end of described second optical fiber collimator (13) is as the first end of described light-splitting device (3), the light that the output terminal of described second optical fiber collimator (13) exports is as the incident light of described dichroic sheet (16);
Described first fiber coupler (15) is arranged on the reflected light path of described dichroic sheet (16), and one end of described first fiber coupler (15) is as the second end of described light-splitting device (3), the other end of described first fiber coupler (15) is used for receiving the light reflected through described dichroic sheet (16);
Described second fiber coupler (17) is arranged on the transmitted light path of described dichroic sheet (16), and one end of described second fiber coupler (17) is as the 3rd end of described light-splitting device (3), the other end of described second fiber coupler (17) is used for receiving the light through the transmission of described dichroic sheet (16).
5. fluorescence detection device as claimed in claim 4, it is characterized in that, described light-splitting device (3) also comprises the second optical filter (19) be arranged between described second optical fiber collimator (13) and described dichroic sheet (16).
6. fluorescence detection device as claimed in claim 4, it is characterized in that, described light-splitting device (3) comprises the 3rd optical filter (20) also comprising and being arranged between described dichroic sheet (16) and described second fiber coupler (17).
7. a fluorescence detection device, it is characterized in that, comprise fiber coupled laser (8), excitation fiber (2), the second optical fiber collimator (13), the first fiber coupler (15), dichroic sheet (16), the 3rd optical filter (20), detection optical fiber (4) and photomultiplier (12);
Described second optical fiber collimator (13) is arranged in the input path of described dichroic sheet (16), and the input end of described second optical fiber collimator (13) connects described fiber coupled laser (8) by described excitation fiber (2);
Described first fiber coupler (15) is arranged on the reflected light path of described dichroic sheet (16), and one end of described first fiber coupler (15) connects testing sample (5) by described detection optical fiber (4);
Described 3rd optical filter (20) and described photomultiplier (12) are successively set on the transmitted light path of described dichroic sheet (16).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520835340.6U CN205038162U (en) | 2015-10-26 | 2015-10-26 | Fluorescence detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520835340.6U CN205038162U (en) | 2015-10-26 | 2015-10-26 | Fluorescence detection device |
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| CN205038162U true CN205038162U (en) | 2016-02-17 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010954A (en) * | 2016-05-05 | 2016-10-12 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel microdroplet type digital PCR optical detection system, device and method |
| CN108414480A (en) * | 2018-01-26 | 2018-08-17 | 中国海洋石油集团有限公司 | A kind of crude oil fluorescence measuring device and method |
| CN110285999A (en) * | 2019-07-08 | 2019-09-27 | 肯维捷斯（武汉）科技有限公司 | A kind of solidliquid mixture sampler and its sampling method |
| CN113720825A (en) * | 2021-11-04 | 2021-11-30 | 四川丹诺迪科技有限公司 | Optical instant detector and detection method and application |
-
2015
- 2015-10-26 CN CN201520835340.6U patent/CN205038162U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010954A (en) * | 2016-05-05 | 2016-10-12 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel microdroplet type digital PCR optical detection system, device and method |
| CN106010954B (en) * | 2016-05-05 | 2018-07-20 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel droplet type digital pcr Systems for optical inspection, device and method |
| CN108414480A (en) * | 2018-01-26 | 2018-08-17 | 中国海洋石油集团有限公司 | A kind of crude oil fluorescence measuring device and method |
| CN110285999A (en) * | 2019-07-08 | 2019-09-27 | 肯维捷斯（武汉）科技有限公司 | A kind of solidliquid mixture sampler and its sampling method |
| CN113720825A (en) * | 2021-11-04 | 2021-11-30 | 四川丹诺迪科技有限公司 | Optical instant detector and detection method and application |
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Granted publication date: 20160217 |