CN204462021U - Fluorescence analyser - Google Patents
Fluorescence analyser Download PDFInfo
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- CN204462021U CN204462021U CN201520122705.0U CN201520122705U CN204462021U CN 204462021 U CN204462021 U CN 204462021U CN 201520122705 U CN201520122705 U CN 201520122705U CN 204462021 U CN204462021 U CN 204462021U
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Abstract
The utility model discloses a kind of fluorescence analyser, comprise signal generating system, light source, sample cell, detecting device and fibre-optic transmission system (FOTS), wherein, signal generating system is for generation of a current signal; The current signal that light source is used for producing according to described signal generating system induces an exciting light; The exciting light that sample cell is used for producing according to described light source goes out a fluorescence to sample excitation wherein; The fluorescence conversion that detecting device is used for being produced by described sample cell is can for the electric signal detected; And the exciting light extremely described sample cell that fibre-optic transmission system (FOTS) produces for transmitting described light source, and transmit the fluorescence extremely described detecting device of described sample cell generation.Fluorescence analyser of the present utility model has that structure is simple, easy to operate, light path is stablized, without strong, the highly sensitive feature of electromagnetic interference (EMI), anti-noise ability.
Description
Technical field
The utility model relates to field of biological detection, particularly a kind of fluorescence analyser.
Background technology
Along with being on the rise of ecological environmental pollution, the problem in recent years in environmental monitoring gets most of the attention, and all kinds of analytical instrument obviously act as indispensable role wherein.
In all kinds of analytical instrument, especially with callophane, because its detection sensitivity is relatively high, selectivity is comparatively wide and have the advantages such as very high response stability, and is widely used, the application especially in planktonic classification and Concentration Testing.
As shown in Figure 1, at present, callophane forms primarily of four basic elements of character: light source, sample cell, single two color system and detecting device.Light source forms monochromatic excitation light path to sample cell through single two color system, and the sample in sample cell sends fluorescence and is collected importing detecting device, and the signal recorder be connected with detecting device is then mainly used in record analysis.Wherein, single two color system all comprises monochromator or optical filter, lens usually.
But, in fluorescence analyser, use lens or optical filter to remain in the following defect continually:
(1) lens: the basic parameter of lens is focal length, numerical aperture and operating distance.The reflection loss of ordinary lens is larger, and especially for the fluorescence analyser using multiple lens, the cumulative meeting of reflection loss seriously reduces luminous flux, coats coating (the such as MgF of refractive index between the two between glass and air
2), although reflection loss can greatly reduce, the lens that reflection loss is less, its price is also more expensive.And adopting the fluorescence analyser of poly-lens, its structure is extremely loaded down with trivial details, and the focal length calibration of lens is also cumbersome, and reflection loss when multi-beam is assembled can reduce luminous flux further.Meanwhile, because this kind of fluorescence analyser needs energising to use, so inevitably there is electromagnetic interference (EMI), the anti-noise ability of system is reduced.
(2) optical filter: if adopt absorption-type optical filter (such as coloured glass, crystal, sinter or film) to absorb, although this kind of optical filter is easy to use, price is lower and angle requirement for incident light is not high, transmitance is relatively low; And another kind of interference filter, although transmitance is relatively high, narrower through wavelength spectral band, price is more expensive, and maximumly can change with the change of incident light through wavelength.
In addition, when detecting desired active substance content and being very low, material detect easily disturb by the matrix of complicated component, and cause the sensitivity of fluorescence analyser relatively inadequate.
Utility model content
The purpose of this utility model is to provide a kind of fluorescence analyser, multiple lens or optical filter is generally used for solving fluorescence analyser of the prior art, and cause the problem of complex structure, electromagnetic interference (EMI), anti-noise ability difference, and relatively detect desired active substance content very low time insufficient sensitivity problem.
In order to solve the problems of the technologies described above, the utility model provides following technical scheme:
The utility model provides a kind of fluorescence analyser, comprises signal generating system, light source, sample cell, detecting device and fibre-optic transmission system (FOTS), and wherein, signal generating system is for generation of a current signal; The current signal that light source is used for producing according to described signal generating system induces an exciting light; The exciting light that sample cell is used for producing according to described light source goes out a fluorescence to sample excitation wherein; The fluorescence conversion that detecting device is used for being produced by described sample cell is can for the electric signal detected; And the exciting light extremely described sample cell that fibre-optic transmission system (FOTS) produces for transmitting described light source, and transmit the fluorescence extremely described detecting device of described sample cell generation.
Preferably, described signal generating system comprises signal generator and current feedback circuit, described signal generator is for generation of a periodically variable waveform signal, and the waveform signal that described current feedback circuit is used for producing according to described signal generator produces described current signal.
Preferably, described signal generator is sinusoidal signal generator.
Preferably, described light source is array light-emitting diode.
Preferably, described detecting device comprises electrooptical device and signal processing circuit, and the fluorescence conversion that described electrooptical device is used for described sample cell to produce is described electric signal, and described signal processing circuit is used for described electric signal to amplify rear output.
Preferably, described electrooptical device is photomultiplier, and described signal processing circuit is trans-impedance amplifier.
Preferably, described fluorescence analyser also comprises an optical filter, and the fluorescence that described sample cell produces, after described optical filter, transfers to described electrooptical device
Preferably, described fibre-optic transmission system (FOTS) comprises launching fiber group and collects optical fiber, the exciting light extremely described sample cell that described launching fiber group produces for transmitting described light source, the fluorescence extremely described detecting device that described collection optical fiber produces for transmitting described sample cell.
Preferably, the probe that described launching fiber group comprises three reception optical fiber, fiber coupler, a Transmission Fibers and are arranged at described Transmission Fibers end, this three receptions optical fiber is after the exciting light receiving the generation of described light source, be coupled into light beam by fiber coupler and transfer to described Transmission Fibers, and transferred in described sample cell by described probe.
Preferably, described fluorescence analyser also comprises capture card, and it is connected between described detecting device and a signal recorder, for gathering the electric signal that described detecting device produces, and transmits it to described signal recorder.
The technical scheme provided from above the utility model, compared with prior art, fluorescence analyser of the present utility model, there is following beneficial effect: by signalization generation systems in fluorescence analyser and launching fiber group, and lock-in amplifier is set in signal recorder, this fluorescence analyser is had, and structure is simple, easy to operate, light path is stablized, without strong, the highly sensitive feature of electromagnetic interference (EMI), anti-noise ability.
Accompanying drawing explanation
In order to be illustrated more clearly in each embodiment of the utility model or technical scheme of the prior art, be briefly described to the accompanying drawing used required in each embodiment of the utility model or description of the prior art below.Apparently, the accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the basic element of character schematic diagram of the fluorescence analyser of prior art.
Fig. 2 is the basic element of character schematic diagram of fluorescence analyser of the present utility model.
Fig. 3 is the structural representation of the fluorescence analyser of the utility model one embodiment.
Fig. 4 is the structural representation of the fibre-optic transmission system (FOTS) of another embodiment of the utility model.
Fig. 5 is the detection spectrogram of fluorescence analyser of the present utility model.
Wherein, description of reference numerals is as follows:
100, fluorescence analyser; 1, signal generating system; 11, signal generator; 12, current feedback circuit; 2, light source; 3, fibre-optic transmission system (FOTS); 31, launching fiber group; 311, optical fiber is received; 312, coupling fiber; 313, Transmission Fibers; 314, pop one's head in; 32, optical fiber is collected; 4, sample cell; 41, sample; 5, detecting device; 51, electrooptical device; 52, signal processing circuit; 6, capture card; 7, signal recorder; 71, lock-in amplifier; 8, optical filter.
Embodiment
Technical scheme in the utility model is understood better in order to make those of ordinary skill in the art, and enable above-mentioned purpose of the present utility model, feature and advantage become apparent more, below in conjunction with accompanying drawing, the technical scheme in each embodiment of the present utility model is elaborated further.
Refer to Fig. 2, a kind of fluorescence analyser 100 that the utility model provides comprises: signal generating system 1, light source 2, sample cell 4, detecting device 5 and fibre-optic transmission system (FOTS) 3.Wherein, signal generating system 1 is for generation of a current signal.Light source 2 induces an exciting light for the current signal produced according to signal generating system 1.Sample cell 4 goes out a fluorescence for the exciting light produced according to light source 2 to sample excitation wherein.Detecting device 5 is can for the electric signal detected for the fluorescence conversion produced by sample cell 4.The exciting light that fibre-optic transmission system (FOTS) 3 produces for transmission light source 2 to sample cell 4, and transmits fluorescence that sample cell 4 produces to detecting device 5.
In one embodiment, light source 2 is array light-emitting diode, especially has the LED array of indigo plant, green, amber tri-color beam.
In addition, detecting device 5 is connected with a signal recorder 7, and this signal recorder 7 is mainly used in carrying out record analysis to the electric signal of Gong the detection that detecting device 5 produces.In different embodiments, according to the needs of practical application scene, such as to the requirement of accuracy of detection, detection speed etc., signal recorder 7 can be the terminal device such as computing machine or central processing unit, also can be the numerical processor chips such as MCU chip, fpga chip or dsp chip.
Fig. 3 is the structural representation of the fluorescence analyser 100 of the utility model one embodiment.
As shown in Figure 3, in the present embodiment, signal generating system 1 comprises signal generator 11 and current feedback circuit 12.Wherein, signal generator 11 for generation of a periodically variable waveform signal, the waveform signal generation current signal of current feedback circuit 12 for producing according to signal generator 11.
Particularly, signal generator 11 is sinusoidal signal generator, and can be used for producing frequency is the sine wave-shaped signal of 1000Hz.
Current feedback circuit 12 is accurate constant-current drive circuit, and the waveform signal that can produce according to signal generator 11 produces identical with its frequency and has the current signal of certain phase relation, produces to bring out light source 2 further the exciting light that same period changes.
By signal generating system 1, the amplitude of the exciting light that light source 2 is produced can follow the changes in amplitude of the waveform signal that signal generator 11 produces, and then the fluorescence that sample cell 4 is produced also can be followed the amplitude of the waveform signal that signal generator 11 produces and change.Because above-mentioned three kinds of signals have identical periodic changes in amplitude, therefore can reduce other parasitic lights without same magnitude change to the interference of system, thus improve signal to noise ratio (S/N ratio), improve the anti-noise ability of system.
Detecting device 5 comprises electrooptical device 51 and signal processing circuit 52.Wherein, electrooptical device 51 is electric signal for the fluorescence conversion produced by sample cell 4, and signal processing circuit 52 exports after being amplified by this electric signal.
Particularly, electrooptical device 51 is photomultiplier, and signal processing circuit 52 is trans-impedance amplifier.By trans-impedance amplifier to the modulation of electric signal and amplification, improve the detection sensitivity of fluorescence analyser 100 pairs of feeble signals.
In addition, in the present embodiment, fluorescence analyser 100 also comprises an optical filter 8.The fluorescence that sample cell 4 produces, after this optical filter 8, just transfers to electrooptical device 51 by fibre-optic transmission system (FOTS) 3.Certainly, the effect of optical filter 8 is the light beam of radiation wave band needed for leaching.In other embodiments, according to the needs of different application scenarioss, as long as the fluorescence that sample cell 4 produces can meet the demands, this fluorescence analyser 100 also can not need this optical filter 8, thus the quantity of optical filter 8 is reduced to 0, not only greatly can reduce the incident angle to the fluorescence that sample cell 4 produces and requirement of strength, and greatly can reduce manufacturing cost.
Fibre-optic transmission system (FOTS) 3 comprises launching fiber group 31 and collects optical fiber 32.Wherein, the exciting light that launching fiber group 31 produces for transmission light source 2, to sample cell 4, collects optical fiber 32 for the fluorescence that transmits sample cell 4 and produce to detecting device 5.Optical fiber has that numerical aperture is large, resolution is high and the feature such as flexible, and the pliability of itself can make light beam be propagated by curved path, by substituting poly-lens of the prior art with fibre-optic transmission system (FOTS) 3, not only reduce reflection loss, improve luminous flux, and enormously simplify the structure of fluorescence analyser 100, reduce manufacturing cost.
In the present embodiment, fluorescence analyser 100 also comprises capture card 6, it is connected between detecting device 5 and signal recorder 7, the waveform signal that the signal generator 11 for collection signal generation systems 1 produces and the electric signal that detecting device 5 produces, and the two is transferred to signal recorder 7.
Further, signal recorder 7 comprises lock-in amplifier 71 and uses to coordinate signal generating system 1, and this lock-in amplifier 71 can be multichannel digital lock-in amplifier.The waveform signal that the signal generator 11 of signal generating system 1 produces by lock-in amplifier 71 is as reference signal, and with this reference signal for benchmark, the electric signal produce detecting device 5 and measured signal are modulated and amplify, to improve the detection sensitivity to feeble signal further.
In the present embodiment, fluorescence analyser 100 also comprises D.C. regulated power supply (not shown), for providing a working power voltage to signal generating system 1, to make signal generating system 1, especially current feedback circuit 12 is normally worked generation one current signal.
Fig. 4 is the structural representation of the fibre-optic transmission system (FOTS) 3 of another embodiment of the utility model.
As shown in Figure 4, in the present embodiment, the probe 314 that launching fiber group 31 comprises three root receiving fibers 311, fiber coupler 312, Transmission Fibers 313 and is arranged at Transmission Fibers 313 end.
Corresponding light source 2 for have indigo plant, green, amber tri-color beam LED array time, after above-mentioned three root receiving fibers 311 can receive three look exciting lights of this light source 2 generation respectively, light beam is coupled into by fiber coupler 312, make light source 2 can transfer to Transmission Fibers 313 with more assembling, and the sample 41 that transfers in sample cell 4 is continued by the probe 314 of this Transmission Fibers 313 end, inspire after corresponding fluorescence until sample 41 and transfer to collection optical fiber 32, and finally transfer to detecting device 5.
Certainly, in other embodiments, if when corresponding light source 2 is monochromatic source, a wherein root receiving fiber 311 can be only used to carry out receiving to reduce service wear, also three root receiving fibers 311 can be used to carry out receiving to improve light signal strength and stability of layout, and the utility model is not as limit simultaneously.
It is worth mentioning that, in the present embodiment, be provided with probe 314 at the end of Transmission Fibers 313, make fluorescence analyser 100 can not produce any electromagnetic interference (EMI).Unlike the prior art, probe 314 does not arrange any circuit, even if sample 41 is in water, also electromagnetic interference (EMI) can not be produced because probe 314 directly stretches into hydrospace detection, and the miscellaneous part of fluorescence analyser 100 does not need into water, even if energising also still can not bring any electromagnetic interference (EMI).
Existing composition graphs 2-4, the principle of work of the fluorescence analyser 100 in an embodiment concrete to the utility model is explained.
Sinusoidal signal generator produces a sine wave-shaped signal to accurate constant-current drive circuit, after accurate constant-current drive circuit is powered by D.C. regulated power supply, follows this sine wave-shaped signal and produces a sinusoidal current signal, and be sent to light source 2.
Light source 2 is for having the LED array of indigo plant, green, amber tri-color beam, and it induces three beams by sinusoidal current signal is periodically variable exciting light.By this light source 2 to three root receiving fibers 311 in the launching fiber group 31 of fibre-optic transmission system (FOTS) 3, after importing indigo plant, green, amber three beams exciting light respectively into, light beam is coupled into by fiber coupler 312, and by Transmission Fibers 313, this Shu Guang being converged to the probe 314 of this Transmission Fibers 313 end, this probe 314 directly stretches into detection sample 41 wherein in sample cell 4.
The fluorescence that sample 41 is inspired passes through to collect optical fiber 32 and is collected into photomultiplier, is converted to by photomultiplier and for the electric signal detected, and can exports capture card 6 to after trans-impedance amplifier modulation and amplification by this fluorescence.
Capture card 6 is not only for the electric signal of Gong the detection of acquisition testing device 5 generation, simultaneously also for the sine wave-shaped signal of collection signal generator 11 generation, and the two is transferred to respectively the lock-in amplifier 71 of signal recorder 7, in lock-in amplifier 71, utilize modulation technique to carry out detection to the signal collected analyze.Such as, to the detection spectrum of chlorophyll fluorescence as shown in Figure 5, under physiological temp, the wavelength peak of chlorophyll fluorescence is approximately the ruddiness of 685nm, and unanimously extends to the far-red light place of 800nm.
Visible, produce sinusoidal current signal at signal generating system 1, and bring out exciting light amplitude formed cyclical variation time, sample 41 excite the amplitude of the fluorescence of generation also can form identical cyclical variation.Again by lock-in amplifier 71, its measured signal is made to be able near exciting light modulating frequency, export with extremely narrow width, and then make to only have identical with the signal frequency of reference signal and the signal content with certain phase relation just can arrive photomultiplier, thus reduce the interference of other parasitic light to system, improve signal to noise ratio (S/N ratio), improve the anti-noise ability of system.
In sum, fluorescence analyser of the present utility model has that structure is simple, easy to operate, light path is stablized, without strong, the highly sensitive feature of electromagnetic interference (EMI), anti-noise ability, can detect detect desired active substance content very low and easily the material that disturbs by the matrix of complicated component.
Foregoing, is only preferred embodiment of the present utility model, does not form the restriction to the utility model protection domain.Any do within spirit of the present utility model and principle amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (10)
1. a fluorescence analyser, is characterized in that, comprising:
Signal generating system, for generation of a current signal;
Light source, the current signal for producing according to described signal generating system induces an exciting light;
Sample cell, goes out a fluorescence for the exciting light produced according to described light source to sample excitation wherein;
Detecting device, the fluorescence conversion for being produced by described sample cell is can for the electric signal detected; And
Fibre-optic transmission system (FOTS), for transmitting the exciting light extremely described sample cell that described light source produces, and transmits the fluorescence extremely described detecting device of described sample cell generation.
2. fluorescence analyser as claimed in claim 1, it is characterized in that, described signal generating system comprises signal generator and current feedback circuit, described signal generator is for generation of a periodically variable waveform signal, and the waveform signal that described current feedback circuit is used for producing according to described signal generator produces described current signal.
3. fluorescence analyser as claimed in claim 2, it is characterized in that, described signal generator is sinusoidal signal generator.
4. fluorescence analyser as claimed in claim 1, it is characterized in that, described light source is array light-emitting diode.
5. fluorescence analyser as claimed in claim 1, it is characterized in that, described detecting device comprises electrooptical device and signal processing circuit, the fluorescence conversion that described electrooptical device is used for described sample cell to produce is described electric signal, and described signal processing circuit is used for described electric signal to amplify rear output.
6. fluorescence analyser as claimed in claim 5, it is characterized in that, described electrooptical device is photomultiplier, and described signal processing circuit is trans-impedance amplifier.
7. fluorescence analyser as claimed in claim 5, is characterized in that, also comprise an optical filter, and the fluorescence that described sample cell produces, after described optical filter, transfers to described electrooptical device.
8. fluorescence analyser as claimed in claim 1, it is characterized in that, described fibre-optic transmission system (FOTS) comprises launching fiber group and collects optical fiber, the exciting light extremely described sample cell that described launching fiber group produces for transmitting described light source, the fluorescence extremely described detecting device that described collection optical fiber produces for transmitting described sample cell.
9. fluorescence analyser as claimed in claim 8, it is characterized in that, the probe that described launching fiber group comprises three reception optical fiber, fiber coupler, a Transmission Fibers and are arranged at described Transmission Fibers end, this three receptions optical fiber is after the exciting light receiving the generation of described light source, be coupled into light beam by fiber coupler and transfer to described Transmission Fibers, and transferred in described sample cell by described probe.
10. fluorescence analyser as claimed in claim 1, it is characterized in that, also comprise capture card, it is connected between described detecting device and a signal recorder, for gathering the electric signal that described detecting device produces, and transmits it to described signal recorder.
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| CN201520122705.0U CN204462021U (en) | 2015-03-02 | 2015-03-02 | Fluorescence analyser |
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| CN201520122705.0U CN204462021U (en) | 2015-03-02 | 2015-03-02 | Fluorescence analyser |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010954A (en) * | 2016-05-05 | 2016-10-12 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel microdroplet type digital PCR optical detection system, device and method |
| CN112904526A (en) * | 2021-01-21 | 2021-06-04 | 浙江大学 | High-precision automatic focusing method and device with anti-noise capability based on differential confocal detection |
| CN113218623A (en) * | 2020-02-04 | 2021-08-06 | 均豪精密工业股份有限公司 | Optical detection system |
| CN113261911A (en) * | 2021-05-14 | 2021-08-17 | 天津工业大学 | Fluorescent electronic endoscope imaging system and detection method thereof |
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2015
- 2015-03-02 CN CN201520122705.0U patent/CN204462021U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010954A (en) * | 2016-05-05 | 2016-10-12 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel microdroplet type digital PCR optical detection system, device and method |
| CN106010954B (en) * | 2016-05-05 | 2018-07-20 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Novel droplet type digital pcr Systems for optical inspection, device and method |
| CN113218623A (en) * | 2020-02-04 | 2021-08-06 | 均豪精密工业股份有限公司 | Optical detection system |
| CN112904526A (en) * | 2021-01-21 | 2021-06-04 | 浙江大学 | High-precision automatic focusing method and device with anti-noise capability based on differential confocal detection |
| CN113261911A (en) * | 2021-05-14 | 2021-08-17 | 天津工业大学 | Fluorescent electronic endoscope imaging system and detection method thereof |
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Effective date of registration: 20210508 Address after: 518000 3rd floor, international graduate school, Tsinghua University, high tech park, Nanshan District, Shenzhen City, Guangdong Province Patentee after: He Yonghong Address before: 411, comprehensive service building, Nanshan cloud Valley Innovation Industrial Park, 1183 Liuxian Avenue, Taoyuan Street, Nanshan District, Shenzhen, Guangdong 518000 Patentee before: SHENZHEN SHENGQIANG TECHNOLOGY Co.,Ltd. |
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