CN104990902A - Plant chlorophyll fluorescence detection device based on LED - Google Patents
Plant chlorophyll fluorescence detection device based on LED Download PDFInfo
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- CN104990902A CN104990902A CN201510350314.9A CN201510350314A CN104990902A CN 104990902 A CN104990902 A CN 104990902A CN 201510350314 A CN201510350314 A CN 201510350314A CN 104990902 A CN104990902 A CN 104990902A
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Abstract
The invention discloses a plant chlorophyll fluorescence detection device based on an LED. The plant chlorophyll fluorescence detection device based on the LED comprises a shell, a light source arranged inside the left side of the shell, a beam shaping lens located at the right side of the light source, a blue light narrow band pass filter arranged at the right side of the beam shaping lens, a 45-degree dichroscope arranged at the center position of the shell and a focusing lens with an adjustable height position arranged at the bottom of the shell, a blade fixture window is arranged at the bottom of the shell, and a blade fixture, with a light-pass diaphragm, matched with the blade fixture window is arranged below the blade fixture window; the top of the shell is provided with a silicon photocell, a fluorescence edge filter located right below the silicon photocell and the focusing lens arranged below the fluorescence edge filter. The plant chlorophyll fluorescence detection device based on the LED has the advantages that the structure is simple, a 45-degree spectroscope is adopted to separate a plant chlorophyll fluorescence excitation light path from a fluorescence reception light path, the shaping lens and the focusing lens are used for achieving uniform illumination of the LED blue light excitation light on the premise that the excitation strength is guaranteed, and induction type real-time rapid detection on the plant chlorophyll fluorescence can be conducted.
Description
Technical field
The present invention relates to a kind of pick-up unit, especially a kind of LED-based plant chlorophyll fluorescence detection device.
Background technology
Photosynthesis is to provide the basis of the metabolism of plant all substances and energetic supersession, that green plants utilizes the photosynthetic pigments such as chlorophyll, under the irradiation of visible ray, through light reaction and carbon reaction (being once called as dark reaction), carbon dioxide and water are converted into organism, and discharge the biochemical process of oxygen (or hydrogen).Also there is energy conversion process luminous energy being changed into chemical energy in organism simultaneously.In conversion process of energy, some luminous energy loss discharges with the fluorescence mode of longer wavelength.The change of a lot of photosynthesis can be reflected by chlorophyll fluorescence, and fluorescence measurement techniques does not need to destroy cell, do not injure biosome, therefore studying and detect photosynthetic fluorescent characteristic has very important scientific meaning and using value.At present, chlorophyll fluorescence is widely used in photosynthesis, plant stress physiology, Hydrobiology, thalassography and remote sensing etc.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of compact structure, portable LED-based plant chlorophyll fluorescence detection device quick, easy to use.
The present invention adopts following technical scheme for solving its technical matters: a kind of LED-based plant chlorophyll fluorescence detection device, its gordian technique is: it comprises shell, be located at the light source of excited chlorophyll fluorescence ability inner on the left of shell, be positioned horizontally in the beam shaping lens on the right side of light source, be placed in the blue light narrow band pass filter for limiting plant chlorophyll exciting light wave band on the right side of beam shaping lens, be located at 45 ° of dichroic mirrors for reflect blue and transmission plant fluorescence of housing center position and be located at outer casing bottom for exciting the condenser lens of the varying height positions of plant fluorescence, the bottom of described shell is provided with blade fixture window, described blade fixture beneath window is provided with the blade fixture with logical light diaphragm matched, described cover top portion being provided with silicon photocell for measuring fluorescence intensity, being placed in the fluorescence cutoff filter immediately below silicon photocell and being placed in the condenser lens for collecting fluorescence below fluorescence cutoff filter.
Preferably, described condenser lens is fixed on in externally threaded lens hanger, and the bottom of described shell is provided with positioning tube, and this positioning tube is provided with the internal thread supporting with described external thread, can by changing the chi footpath of the position adjustment chlorophyll exciting light of condenser lens.
Preferably, described blade fixture therebetween holds blade, described blade fixture be provided with blade fixture Window match, the chlorophyll that can adjust blade as required excites the logical light diaphragm of area.
Preferably, be provided with for ensureing airtight space O-ring seal between described blade fixture window and blade fixture.
The beneficial effect adopting technique scheme to produce is as follows: the present invention adopts LED as excitation source, can install the LED light source of different-waveband as required, and adjust excitation beam by beam shaping lens; The present invention adopts 45 ° of dichroic mirrors exciting light and fluorescence to be designed along separate routes, improves the compactness of device on the one hand, achieves path-splitting design on the other hand, improves the convenient of fluorescent collecting; The present invention adopt establish threaded positioning tube, and coordinate the logical light diaphragm of blade fixture can according to measure need adjust blade excite area.It is adjustable that the present invention has exciting light density, measures blade area adjustable, light channel structure compact ingenious, measure the features such as convenient, the metering circuit in conjunction with peripheral hardware can the Quick Measurement of experimental plant leaf chlorophyll fluorescence.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Wherein, 1, light source; 2, beam shaping lens; 3, narrow band pass filter; 4,45 ° of dichroic mirrors; 5, condenser lens; 6, positioning tube; 8, fluorescence collector lens; 9, cutoff filter; 10, silicon photocell; 11, blade fixture; 12, logical light diaphragm; 13, shell.
Embodiment
below in conjunction with accompanying drawing, the present invention is further detailed.
See accompanying drawing 1, the present embodiment comprises shell 13, be located at the LED light source 1 of excited chlorophyll fluorescence ability inner on the left of shell 13, be positioned horizontally in the beam shaping lens 2 on the right side of LED light source 1, be placed in the blue light narrow band pass filter 3 for limiting plant chlorophyll exciting light wave band on the right side of beam shaping lens 2, be located at 45 ° of dichroic mirrors 4 for reflect blue and transmission plant fluorescence of shell 13 center and be located at bottom shell 13 for exciting the condenser lens 5 of the varying height positions of plant fluorescence, the bottom of described shell is provided with blade fixture window, described blade fixture beneath window be provided with match with logical light diaphragm 12(also can cry adjustment diaphragm) blade fixture 11, described shell 13 top being provided with silicon photocell 10 for measuring fluorescence intensity, being placed in the fluorescence cutoff filter 9 immediately below silicon photocell 10 and being placed in the condenser lens 8 for collecting fluorescence below fluorescence cutoff filter 9.
Described LED light source realizes diverging light to be shaped as collimated light beam by the beam shaping lens 2 be placed horizontally on the right side of it, the wavelength control of the light beam after shaping is being excited the 460nm-480nm wave band of plant chlorophyll fluorescence by described narrow band pass filter 3, 45 ° of described dichroic mirrors are the center that 45° angle is positioned over shell 13, LED blue light paths is carried out 45 ° of turnovers by horizontal direction launch vertically downward, logical light diaphragm 12 position of blade fixture 11 is irradiated to after being focused on by condenser lens 5, condenser lens 5 can adjust its position in the optical path by the adjustment position of lens hanger in positioning tube 6, and then can need to change the excitation light density being irradiated to the hot spot of blade fixture window according to actual Blade measuring, the actual facula area be irradiated on blade can be adjusted again by the size of the logical light diaphragm 12 of adjustment, fluorescence can be sent after vanes blue light illumination excites, fluorescence is by converging after condenser lens 5,45 ° of dichroic mirrors 4 and fluorescence collector lens 8, and then after cutoff filter 9 is by parasitic light filtering, converging to described silicon photocell 10, the signal acquisition processing circuit of recycling peripheral hardware realizes the Quick Measurement of fluorescence intensity.
The window of blade fixture described in the present embodiment and blade fixture 11 mate mutually, and the two passes through seal with elastometic washer.Described light source 1 can adopt LED blue light source, also other wave band light sources possessing equally and excite chlorophyll fluorescence ability can be replaced by as required, corresponding 45 ° of dichroic mirrors 4 also need to carry out corresponding replacing according to the emission wavelength of light source 1, can realize the chlorophyll fluorescence Quick Measurement under different exciting light pattern.Point upper and lower two parts in described blade fixture 11 structure, middle clamping blade, upper part and blade fixture Window match, utilize rubber ring to be embedded into enclosure, ensures sealing; Inside establish window to lead to light diaphragm 12, the size of diaphragm can be adjusted as required, and then the chlorophyll of adjustment blade excites area.Further improvement, can arrange one piece of iron plate blocked on logical light diaphragm 12, convenient.
When using the present invention to carry out Quick Measurement to plant chlorophyll fluorescence, the first step needs first to be taken out from shell by blade fixture 11, then measure the position requiring the lens hanger adjustment condenser lens 5 utilized in positioning tube 6 according to the chlorophyll fluorescence of actual blade, and then the hot spot optical density being irradiated to blade clamping window is adjusted to desired strength; Logical light diaphragm 12 on blade fixture 11 is kept closed condition by second step, then blade to be measured is clamped with blade fixture 11, utilize the confined air chien shih of blade fixture 11 blade to be measured to keep darkroom state certain hour, then measurement mechanism is connected with blade fixture 11 is airtight by blade fixture window; 3rd step, excite area to open logical light diaphragm 12 according to the blade of practical measurement requirement and adjust diaphragm open-mouth ruler footpath, the metering circuit switch opening peripheral hardware carries out chlorophyll fluorescence Quick Measurement, and the metering circuit of peripheral hardware adopts existing equipment or components and parts to realize here.
It is adjustable that the present invention has exciting light density, measures blade area adjustable, light channel structure compact ingenious, measure the features such as convenient, the metering circuit in conjunction with peripheral hardware can the induction type Quick Measurement of experimental plant leaf chlorophyll fluorescence.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein when not departing from the spirit or scope of the invention, can realize in other embodiments.Therefore, the invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (5)
1. a LED-based plant chlorophyll fluorescence detection device, it is characterized in that: it comprises shell (13), be located at the light source (1) of inner the excited chlorophyll fluorescence ability in shell (13) left side, be positioned horizontally in the beam shaping lens (2) on light source (1) right side, be placed in the blue light narrow band pass filter (3) for limiting plant chlorophyll exciting light wave band on beam shaping lens (2) right side, be located at 45 ° of dichroic mirrors (4) for reflect blue and transmission plant fluorescence of shell (13) center and be located at shell (13) bottom for exciting the condenser lens (5) of the varying height positions of plant fluorescence, the bottom of described shell is provided with blade fixture window, described blade fixture beneath window is provided with the blade fixture (11) with logical light diaphragm (12) matched, described shell (13) top being provided with silicon photocell (10) for measuring fluorescence intensity, being placed in the fluorescence cutoff filter (9) immediately below silicon photocell (10) and being placed in the condenser lens (8) for collecting fluorescence of fluorescence cutoff filter (9) below.
2. LED-based plant chlorophyll fluorescence detection device according to claim 1, it is characterized in that: described condenser lens (5) is fixed on in externally threaded lens hanger, the bottom of described shell (13) is provided with positioning tube (6), and this positioning tube (6) is provided with the internal thread supporting with described external thread.
3. LED-based plant chlorophyll fluorescence detection device according to claim 1, it is characterized in that: described blade fixture (11) therebetween holds blade, described blade fixture (11) be provided with blade fixture Window match, the chlorophyll that can adjust blade as required excites the logical light diaphragm (12) of area.
4. LED-based plant chlorophyll fluorescence detection device according to claim 3, is characterized in that: be provided with for ensureing airtight space O-ring seal between described blade fixture window and blade fixture (11).
5. LED-based plant chlorophyll fluorescence detection device according to claim 1, is characterized in that: described light source (1) is blue light source or red-light source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510350314.9A CN104990902A (en) | 2015-06-24 | 2015-06-24 | Plant chlorophyll fluorescence detection device based on LED |
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| CN201510350314.9A CN104990902A (en) | 2015-06-24 | 2015-06-24 | Plant chlorophyll fluorescence detection device based on LED |
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| CN104990902A true CN104990902A (en) | 2015-10-21 |
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| CN201510350314.9A Pending CN104990902A (en) | 2015-06-24 | 2015-06-24 | Plant chlorophyll fluorescence detection device based on LED |
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Cited By (9)
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| CN105548128A (en) * | 2016-02-20 | 2016-05-04 | 中国科学院烟台海岸带研究所 | Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method |
| CN106324237A (en) * | 2016-08-18 | 2017-01-11 | 北京乐普医疗科技有限责任公司 | Scanning device for quantitative fluorescence immunoassay |
| CN106770091A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of isothermal portable fluorescence detector for detecting coronary heart disease |
| CN108195804A (en) * | 2017-12-12 | 2018-06-22 | 濮阳光电产业技术研究院 | A kind of fluorescent probe |
| CN108550685A (en) * | 2018-03-19 | 2018-09-18 | 山东省科学院海洋仪器仪表研究所 | A kind of special LED of chlorophyll fluorescence excitation |
| CN109406462A (en) * | 2018-10-08 | 2019-03-01 | 新视界视光健康产业（南京）有限公司 | A kind of eyeglass blue light filter efficiency detection device |
| GB2568307A (en) * | 2017-11-14 | 2019-05-15 | Stratec Biomedical Ag | Spectral excitation device |
| CN113252240A (en) * | 2021-05-11 | 2021-08-13 | 西北工业大学 | Portable optical pressure sensitive coating static pressure calibration cabin and calibration method |
| CN118010658A (en) * | 2024-04-10 | 2024-05-10 | 山东来因光电科技有限公司 | Automatic chlorophyll content measuring device |
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| CN105548128A (en) * | 2016-02-20 | 2016-05-04 | 中国科学院烟台海岸带研究所 | Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method |
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| CN106324237B (en) * | 2016-08-18 | 2018-03-06 | 北京乐普医疗科技有限责任公司 | A kind of fluorescence immunoassay quantitative analysis scanning means |
| CN106770091A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of isothermal portable fluorescence detector for detecting coronary heart disease |
| GB2568307A (en) * | 2017-11-14 | 2019-05-15 | Stratec Biomedical Ag | Spectral excitation device |
| CN108195804A (en) * | 2017-12-12 | 2018-06-22 | 濮阳光电产业技术研究院 | A kind of fluorescent probe |
| CN108550685A (en) * | 2018-03-19 | 2018-09-18 | 山东省科学院海洋仪器仪表研究所 | A kind of special LED of chlorophyll fluorescence excitation |
| CN109406462A (en) * | 2018-10-08 | 2019-03-01 | 新视界视光健康产业（南京）有限公司 | A kind of eyeglass blue light filter efficiency detection device |
| CN113252240A (en) * | 2021-05-11 | 2021-08-13 | 西北工业大学 | Portable optical pressure sensitive coating static pressure calibration cabin and calibration method |
| CN118010658A (en) * | 2024-04-10 | 2024-05-10 | 山东来因光电科技有限公司 | Automatic chlorophyll content measuring device |
| CN118010658B (en) * | 2024-04-10 | 2024-07-12 | 山东来因光电科技有限公司 | Automatic chlorophyll content measuring device |
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Application publication date: 20151021 |