CN203310753U

CN203310753U - Multi-wavelength capillary electrophoresis fluorescence excitation device

Info

Publication number: CN203310753U
Application number: CN2013202951939U
Authority: CN
Inventors: 叶青; 梅剑春; 田建国
Original assignee: Nankai University
Current assignee: Nankai University
Priority date: 2013-05-24
Filing date: 2013-05-24
Publication date: 2013-11-27
Anticipated expiration: 2023-05-24

Abstract

The utility model discloses a multi-wavelength capillary electrophoresis fluorescence excitation device. The device consists of a plurality of groups of excitation light sources, a multi-path optical fiber coupler and an output optical fiber; each group of excitation light sources consists of a high-brightness light emitting diode, a focusing device and a coupling optical fiber; the input end of the multi-path optical fiber coupler is the coupling optical fiber of each group of the excitation light sources; the light rays of each path in the input end are output by the output optical fiber after being converged and coupled; the output end of the output optical fiber directly extends into the position of a detection window from a detection port of the capillary of an electrophoresis apparatus, so that fluorescence excitation can be performed by irradiating from the inside of the detection window. The wave bands of the high-brightness light emitting diodes adopted by each group of the excitation light sources are inconsistent, and visible excitation light or the excitation light in near-ultraviolet regions of various band waves can be emitted by the multi-wavelength capillary electrophoresis fluorescence excitation device by supplying power to different light emitting diodes, so that the device can be adaptive to the maximum excitation wavelength of different fluorescent probes. Wave bands can be switched by utilizing an electronic means, so that the multi-wavelength capillary electrophoresis fluorescence excitation device has the characteristics of simple structure, low cost and high stability.

Description

Multi-wavelength Capillary Electrophoresis fluorescence excitation device

Technical field

The utility model belongs to the biological chemistry Micrometry, is specifically related to a kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device.

Background technology

High performance capillary electrophoresis (High-performance capillary electrophoresis, HPCE) be one of the fastest analytical approach of development in recent years, has separation efficiency high, fast and the amount of samples of velocity of separation such as lacks at the advantage, is widely used in the fields such as peptide and protein, environment food, DNA sequence analysis and unicellular monitoring.Because active volume and the sample size of HPCE are few, detecting device is had to very high requirement, apply at present the more method that uv absorption, galvanochemistry, mass spectrum and fluoroscopic examination are arranged.Fluoroscopic examination is as the technology of a kind of high sensitivity, high selectivity, and application is very extensive in trace and microscratch sample detection.

Fluorescent capillary electrophoresis tube instrument mainly consists of capillary electrophoresis, fluorescence detector, fluorescence excitation light source.At present, excitation source adopts gas-discharge lamp, gas energizer light source, semiconductor solid state laser etc. usually, but has the characteristics that size is large, energy consumption is high, cost is expensive.In recent years, high brightness LED (1ight-emitting diode, LED) has caused people's concern gradually as a kind of miniature of economy.LED has the advantages such as stable output power, energy consumption is low, volume is little, brightness is high, wavelength coverage is wider, the life-span is long, is suitable as very much fluorescence excitation light source.LED as the excitation source performance close to common coherent source, each wave band of high-brightness LED nearly cover, can, by selecting suitable LED to adapt to the maximum excitation wavelength of fluorescence probe, realize the fluoroscopic examination to biological samples such as protein, DNA and amino acid.LED irradiates the capillary detection window and fluorescence excitation from the kapillary outside after as the optical texture of fluorescence excitation light source, mostly adopting focusing, unavoidably in capillary surface generation anaclasis and scattering process, make the light sent by LED can not be fully for fluorescence excitation, and then cause higher background noise, affect the sensitivity of fluorescence excitation efficiency and capillary electrophoresis apparatus.

The exciting light that LED is sent is introduced into and is positioned at the capillary detection window and realizes fluorescence excitation by light transmitting fiber, have advantages of highly sensitive, background noise is low, choosing wavelength wider range, structure and simple to operate, in the capillary electrophoresis apparatus had, used at present this technology, present way is for different detection material, utilize the different types of LED of mechanical hook-up (being generally round turntable) switching before the condenser lens of optical fiber, select different excitation wavelengths.This mode structure is complicated a little, is unfavorable for miniaturization and the microminiaturization of electrophoresis apparatus, and the anti-seismic performance of instrument and life-span limited by physical construction, easily damage.

Summary of the invention

The purpose of this utility model is to provide a kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device.It has characteristics simple in structure, with low cost, that stability is high.

A kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device (7) that the utility model provides is comprised of many groups excitation source (1), a multi-channel optical fibre coupling mechanism (2) and an output optical fibre (3).Every group of LASER Light Source (1) is comprised of a high brightness LED (4), a focalizer (5) and a coupled fiber (6).The high brightness light emitting diode (4) of every group of excitation source (1) has different emergent light wave bands, the light that high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.

The output terminal of output optical fibre (3) directly stretches into detection window (15) position from the detection port of electrophoresis apparatus kapillary (9), realizes carrying out fluorescence excitation from detection window (15) internal radiation.

By giving respectively different high brightness LED (4) power supplies, the utility model can be launched the exciting light of visible range or each wave band of near ultraviolet region, the maximum excitation wavelength that adapts to different fluorescence probes, realize the fluoroscopic examination to biological samples such as protein, DNA and amino acid.Utilize electronically to replace mechanical hook-up and realize the wave band switching, have characteristics simple in structure, with low cost, that stability is high.

The accompanying drawing explanation

Fig. 1 is the structural drawing of multi-wavelength Capillary Electrophoresis fluorescence excitation device.

Fig. 2 is the use schematic diagram of multi-wavelength Capillary Electrophoresis fluorescence excitation device.

Embodiment

Below in conjunction with accompanying drawing, say the embodiment of multi-wavelength Capillary Electrophoresis fluorescence excitation implement body.

The mechanism of multi-wavelength Capillary Electrophoresis fluorescence excitation device (7) as shown in Figure 1.In Fig. 1,1 is excitation source, and 2 is the multi-channel optical fibre coupling mechanism, and 3 is output optical fibre.Excitation source (1) consists of a high brightness LED (4), a focalizer (5) and a coupled fiber (6), the light that high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.

The effect of multi-channel optical fibre coupling mechanism (2) is that the multichannel input is optically coupled in together, outwards exports.The high brightness light emitting diode (4) of every group of excitation source (1) has different emergent light wave bands.When the high brightness LED (4) in many groups excitation source (1) only has one to light, the wave band of output light is consistent with the wave band of this high brightness LED (4), by electronic switching electricity mode, control the light on and off of high brightness LED (4), just can reach the purpose that changes output optical fibre (3) medium wavelength.

Fig. 2 is the use schematic diagram of multi-wavelength Capillary Electrophoresis fluorescence excitation device, means how multi-wavelength Capillary Electrophoresis fluorescence excitation device (7) uses in the Capillary Electrophoresis fluorescence detector.As shown in Figure 2, the Capillary Electrophoresis fluorescence detector consists of multi-wavelength Capillary Electrophoresis fluorescence excitation device (7), Capillary Electrophoresis part, fluorescence detection device (16) and data processing equipment (17).

Capillary Electrophoresis part: formed by Buffer Pool (8), kapillary (9), detection cell (10), DC high-voltage power supply (11) and platinum electrode (12,13).It is upper that the test section of kapillary (9) is fixed on protective substrate (14), and have detection window (15).The effect of DC high-voltage power supply (11) is to provide electric field, drives the test substance swimming, realizes the efficient separation of material.After micro substance sample introduction to be measured, unidirectional flowing under the effect of electric field force, due to the charge characteristic difference of different materials, thereby suffered electric field force difference, the speed of swimming is also just different, causes the asynchronism(-nization) of material arrival detection window (15), thereby the separation on implementation space.The output terminal of the output optical fibre of multi-wavelength Capillary Electrophoresis fluorescence excitation device directly stretches into the detection window position from electrophoresis apparatus detection port capillaceous, exciting light is incorporated into to kapillary (9) inside, the test substance that irradiating and detecting window (15) is located, realize fluorescence excitation.Fluorescence is collected by fluorescence detection device (16), is transformed into electric signal, then sends data processing equipment (17) to and processes and analyze.

Fluorescence detection device (16) generally is comprised of focusing objective len, optical filter and photomultiplier.After being responsible for fluorescence signal is collected, be transformed into electric signal, while for later analysis, processing.Carrying out opto-electronic conversion is mainly completed by photomultiplier.

Data processing equipment (17) is generally a computing machine, is responsible for gathering and the analysis of fluorescence signal, realizes qualitative and quantitative analysis.

Claims

1. a multi-wavelength Capillary Electrophoresis fluorescence excitation device, be comprised of many groups excitation source (1), a multi-channel optical fibre coupling mechanism (2) and an output optical fibre (3); Every group of LASER Light Source is comprised of a high brightness LED (4), a focalizer (5) and a coupled fiber (6); It is characterized in that: the light that described high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.