CN105004824A - Optical fiber combination device for capillary electrophoresis apparatus - Google Patents
Optical fiber combination device for capillary electrophoresis apparatus Download PDFInfo
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- CN105004824A CN105004824A CN201510508774.XA CN201510508774A CN105004824A CN 105004824 A CN105004824 A CN 105004824A CN 201510508774 A CN201510508774 A CN 201510508774A CN 105004824 A CN105004824 A CN 105004824A
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- optical fiber
- combination device
- call wire
- capillary electrophoresis
- electrophoresis apparatus
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Abstract
The invention discloses an optical fiber combination device for a capillary electrophoresis apparatus. The optical fiber combination device comprises one main optical fiber transmission wire and at least one branch optical fiber transmission wire, wherein each branch optical fiber transmission wire is connected with the main optical fiber transmission wire; a separator for separating different reagent fluorescence signals is arranged on each branch optical fiber transmission wire; the starting end of the main optical fiber transmission wire is connected with an extraction device; detection devices are connected with the end parts of the branch optical fiber transmission wires. The optical fiber combination device can simultaneously detect and analyze a sample and a standard product, so that the detecting time is shortened and the detecting efficiency is improved.
Description
Technical field
The present invention relates to capillary electrophoresis apparatus field, be specifically related to a kind of optical fiber combination device of capillary electrophoresis apparatus.
Background technology
Bioanalysis, such as DNA analysis, carrying out exploring the fast transition with conventional program that is larger and certified reliability from the accurate pure science of pursuit.Capillary Electrophoresis (capillaryelectrophoresis, CE) be a class be split tunnel with kapillary, take high-voltage dc as the Novel liquid-phase isolation technics of driving force, this capillary electrophoresis apparatus can be carried out DNA and be detected and analyze and Separation of Proteins analysis.Existing capillary electrophoresis apparatus is when carrying out detection and analyzing to sample, also need need detect standard items sample detection is forward and backward, again sample and standard items are analyzed, this needs the detection time of twice, work efficiency is lower, when if desired detecting many parts of similar samples, just need to spend more detection time; And the light source adopted during existing capillary electrophoresis apparatus detection mostly is high power laser or LED, the risk that high power laser exists biohazard is comparatively large, and the dispersion of the light ray energy of LED, and monochromaticity is less than laser, thus cause fluorescence excitation efficiency low
Summary of the invention
(1) technical matters that will solve
It is simple that the technical problem to be solved in the present invention is to provide a kind of structure, can detect the optical fiber combination device of the capillary electrophoresis apparatus analyzing sample and standard items simultaneously.
(2) technical scheme
For solving the problems of the technologies described above, the invention provides a kind of optical fiber combination device of capillary electrophoresis apparatus, it comprises a main fiber call wire and at least one point of optical fiber call wire, every bar divides optical fiber call wire to be all connected with main fiber call wire, and every bar divides separation vessel optical fiber call wire is provided with for separating of different reagent fluorescence signal; The top of main fiber call wire is connected with extraction element, and every bar divides the end of optical fiber call wire to be all connected with a pick-up unit.
Wherein, separation vessel is optical filter.
Wherein, pick-up unit is photomultiplier.
(3) beneficial effect
Compared with prior art, the present invention has following features:
(1) the present invention can excite multiple (2 kinds and more than) fluorescence signal simultaneously, efficiently, can detect simultaneously analyze sample and standard items, decrease detection time, improve detection efficiency;
(2) greatly reduce the biohazard risk of high power laser, and its less volume also reduces the requirement to instrument space.
Accompanying drawing explanation
Fig. 1 is the structural representation of the optical fiber combination device of capillary electrophoresis apparatus of the present invention.
In figure, 1-optical fiber combination device; 2-extraction element; 3-pick-up unit; 4-optical filter; 5-kapillary; 6-optical fiber; 11-main fiber call wire; 12-divides optical fiber call wire.
Embodiment
Below in conjunction with drawings and Examples, the specific embodiment of the present invention is described in further detail.Following examples for illustration of the present invention, but can not be used for limiting the scope of the invention.
The structure of the optical fiber combination device of capillary electrophoresis apparatus of the present invention as shown in Figure 1, this optical fiber combination device 1 comprises a main fiber call wire 11 and at least one point of optical fiber call wire 12, every bar divides optical fiber call wire 12 to be all connected with main fiber call wire 11, every bar divides optical fiber call wire 12 to be all provided with separation vessel for separating of different reagent fluorescence signal, is separated by different reagent; The top of main fiber call wire 11 is connected with extraction element 2, and every bar divides the end of optical fiber call wire 12 to be all connected with a pick-up unit 3.In the present embodiment, light splitting fiber optic conduction line 12 is 2.
Above-mentioned separation vessel is optical filter 4, and every bar divides the optical filter of optical fiber call wire 12 all different, only collects a kind of fluorescence signal of reagent, filters out the fluorescence signal of other reagent, to complete the detection analyzing sample and standard items respectively.
Above-mentioned detection device 3 is photomultiplier.
Optical fiber 6 irradiates kapillary 5, the generation fluorescence reaction under the exciting of optical fiber 6 of analysis sample in kapillary 5 and the molecule of standard items, analysis sample fluorescence signal and standard items fluorescence signal to be deposited by optical fiber combination device 1 and are delivered to corresponding pick-up unit 3 and detect by extraction element 2 by fluorescence signal collection more respectively, then are contrasted by the detection data of electric equipment to both.The present invention can detect simultaneously analyze sample and standard items, decreases detection time, improves detection efficiency.
When such as detecting DNA fragmentation, will with cy7 fluorochrome label standard items, test sample product are needed with cy5 fluorochrome label, by standard items and need test sample product to put into same capillary electrophoresis apparatus simultaneously, both molecule electrophoresis in kapillary, generation fluorescence reaction under the exciting through single LASER Light Source, after all fluorescence signals are collected by extraction element, conduct to optical fiber combination device, the 790 nano fluorescent signals collected (i.e. the standard items fluorescence signal of cy7 dye marker) are sent to a pick-up unit and detect by the separation vessel on one point of optical fiber call wire, and the 650 nano fluorescent signals collected (namely sample fluorescence signal surveyed by the need of cy5 dye marker) are sent to the detection of another pick-up unit by the separation vessel on another point of optical fiber call wire, after testing two parts detect data and are analyzed through electric equipment and can judge the whether similar and similarity of two groups of DNA fragmentations.The present invention had not only taken full advantage of the advantages such as LASER Light Source is pure, single, concentration of energy but also had greatly reduced the biohazard risk of high power laser, and its less volume also reduces the requirement to instrument space; Meanwhile, efficiently can excite multiple (2 kinds and more than) fluorescence signal through single LASER Light Source, the Systematic Design for pick-up unit provides wide expanding space simultaneously.
These are only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (3)
1. the optical fiber combination device of a capillary electrophoresis apparatus, it is characterized in that: described optical fiber combination device (1) comprises a main fiber call wire (11) and at least one point of optical fiber call wire (12), described in every bar, a point optical fiber call wire (12) is all connected with described main fiber call wire (11), point optical fiber call wire (12) described in every bar is provided with the separation vessel for separating of different reagent fluorescence signal; The top of described main fiber call wire (11) is connected with extraction element (2), and described in every bar, the end of point optical fiber call wire (12) is all connected with a pick-up unit (3).
2. the optical fiber combination device of capillary electrophoresis apparatus according to claim 1, is characterized in that: described separation vessel is optical filter (4).
3. the optical fiber combination device of capillary electrophoresis apparatus according to claim 1, is characterized in that: described pick-up unit (3) is photomultiplier.
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CN201510508774.XA CN105004824A (en) | 2015-08-18 | 2015-08-18 | Optical fiber combination device for capillary electrophoresis apparatus |
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CN201510508774.XA CN105004824A (en) | 2015-08-18 | 2015-08-18 | Optical fiber combination device for capillary electrophoresis apparatus |
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CN101504371A (en) * | 2009-03-05 | 2009-08-12 | 中国检验检疫科学研究院 | Quantitative determination instrument for melamine content in liquid milk |
CN103134780A (en) * | 2011-12-01 | 2013-06-05 | 中国科学院大连化学物理研究所 | Closely attached type light emitting diode induction fluorescence detector for exciting light path |
CN203191075U (en) * | 2013-03-19 | 2013-09-11 | 杭州晶飞科技有限公司 | Double-beam synchronous triggering micro optical fiber spectrometer |
CN203310753U (en) * | 2013-05-24 | 2013-11-27 | 南开大学 | Multi-wavelength capillary electrophoresis fluorescence excitation device |
CN104062235A (en) * | 2014-07-16 | 2014-09-24 | 北京佰纯润宇生物科技有限公司 | Optical-fiber-based multi-wavelength online detection device of single flow cell |
CN204128940U (en) * | 2014-10-17 | 2015-01-28 | 中国人民解放军第五七二一工厂 | A kind of metal material gross imperfection hot acid corrosion testing device |
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2015
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Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
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US5179606A (en) * | 1989-05-19 | 1993-01-12 | Nec Corporation | Optical coupler |
CN2170511Y (en) * | 1993-10-09 | 1994-06-29 | 南京理工大学 | Burning gas spectrum real time measurer for temp. |
CN2426142Y (en) * | 2000-05-15 | 2001-04-04 | 中国科学院等离子体物理研究所 | High-speed infrared radiation temp. detector |
CN1755351A (en) * | 2004-09-30 | 2006-04-05 | 中国科学院大连化学物理研究所 | LED-induced minisize fluorescence detector |
CN1773257A (en) * | 2005-11-06 | 2006-05-17 | 中国科学院安徽光学精密机械研究所 | Water body pollution laser induction fluorescence remote-measuring method |
CN101504371A (en) * | 2009-03-05 | 2009-08-12 | 中国检验检疫科学研究院 | Quantitative determination instrument for melamine content in liquid milk |
CN103134780A (en) * | 2011-12-01 | 2013-06-05 | 中国科学院大连化学物理研究所 | Closely attached type light emitting diode induction fluorescence detector for exciting light path |
CN203191075U (en) * | 2013-03-19 | 2013-09-11 | 杭州晶飞科技有限公司 | Double-beam synchronous triggering micro optical fiber spectrometer |
CN203310753U (en) * | 2013-05-24 | 2013-11-27 | 南开大学 | Multi-wavelength capillary electrophoresis fluorescence excitation device |
CN104062235A (en) * | 2014-07-16 | 2014-09-24 | 北京佰纯润宇生物科技有限公司 | Optical-fiber-based multi-wavelength online detection device of single flow cell |
CN204128940U (en) * | 2014-10-17 | 2015-01-28 | 中国人民解放军第五七二一工厂 | A kind of metal material gross imperfection hot acid corrosion testing device |
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Application publication date: 20151028 |