CN205774545U - A kind of bacteria detecting apparatus of the micro-fluidic chip realizing PCR and in real time PCR - Google Patents
A kind of bacteria detecting apparatus of the micro-fluidic chip realizing PCR and in real time PCR Download PDFInfo
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- CN205774545U CN205774545U CN201620742561.3U CN201620742561U CN205774545U CN 205774545 U CN205774545 U CN 205774545U CN 201620742561 U CN201620742561 U CN 201620742561U CN 205774545 U CN205774545 U CN 205774545U
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- detecting apparatus
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Abstract
The utility model discloses a kind of micro-fluidic chip realizing PCR and the bacteria detecting apparatus of real-time PCR, described detection device includes photomultiplier tube, described photomultiplier tube is internally provided with optical filter one and optical filter two, described photomultiplier tube bottom is disposed with diaphragm, battery of lens and object lens, two it are provided with to color mirror between described battery of lens and object lens, it is provided with semiconductor laser on the right side of described detection device, being provided with beam expander and optical filter three between described semiconductor laser and detection device, described detection bottom of device is provided with chip.Use semiconductor laser as excitation source, reflect into object lens through dichroic mirror, after being focused on by object lens in the split tunnel of vertical incidence chip, thus inspire fluorescence, improve the efficiency of detection, fluorescence signal is by monitor record, the information of understanding detection that can be real-time after photomultiplier tube is collected simultaneously, improves the efficiency of work.
Description
Technical field
This utility model relates to Bacteria Detection technical field, a kind of micro-fluidic chip realizing PCR and in real time PCR
Bacteria detecting apparatus.
Background technology
PCR is the process realizing DNA cloning in vitro, by polymerase and the primer of particular design, and can be from sample
DNA sequence copies the specific fragment of " significant ", and the quantity amplification of specific fragment can be made to reach 109Times.Use capillary tube
DNA fragmentation is separated by electrophoresis (Capillary Electrophoresis, CE) according to piece size, and detection has specific
The specific fragment of length.CE is the electrophoresis carried out in capillary channel, uses electric field driven DNA molecular, utilizes sieving media simultaneously
With the interaction of DNA molecular, make the DNA fragmentation of different length produce electrophoretic velocity poor, reach to detect the mesh of DNA fragmentation length
's.The generally available higher separation voltage of CE, just can complete to separate within a short period of time.
The operating process that conventional gene is analyzed is comparatively laborious, it usually needs use such as centrifuge, temperature cycler, flat board to coagulate
A series of equipment such as gel electrophoresis instrument, fluorescence imaging analysis instrument or capillary electrophoresis apparatus, this is to the hardware condition of laboratory and experiment
The operation level of personnel is proposed the requirement that comparison is high, the reagent occurred during being not easy to reduce detection and cost of labor.
Further, sample shifts at multiple equipment rooms, adds the probability of operational error and sample contamination.
Utility model content
The purpose of this utility model is to provide the Bacteria Detection dress of a kind of micro-fluidic chip realizing PCR and real-time PCR
Put, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the following technical scheme of this utility model offer: a kind of micro-fluidic chip realizing PCR and reality
Time PCR bacteria detecting apparatus, described detection device includes that photomultiplier tube, described photomultiplier tube are internally provided with optical filter
One is disposed with diaphragm, battery of lens and object lens with optical filter two, described photomultiplier tube bottom, described battery of lens and object lens it
Between be provided with two on the right side of color mirror, described detection device, be provided with semiconductor laser, described semiconductor laser and detection dress
Being provided with beam expander and optical filter three between putting, described detection bottom of device is provided with chip.
Further, described chip is provided with split tunnel, described split tunnel both sides are respectively arranged with buffer pool
Giving up pond with buffer, described split tunnel upper and lower sides is respectively arranged with sample and gives up pond liquid and sample cell.
Further, the position of described semiconductor laser with two between color mirror mutually the most corresponding.
Further, described rush liquid pool, buffer gives up pond, sample gives up pond liquid, sample cell all connect power supply by platinum electrode.
Further, described chip is the micro-fluidic chip of PCR.
Compared with prior art, the beneficial effects of the utility model are:
The bacteria detecting apparatus of a kind of micro-fluidic chip realizing PCR of the present utility model and in real time PCR, uses quasiconductor
Laser instrument, as excitation source, reflects into object lens through dichroic mirror, after being focused on by object lens in the split tunnel of vertical incidence chip,
Thus inspire fluorescence, improve the efficiency of detection, simultaneously fluorescence signal after photomultiplier tube is collected by monitor record, can
The information understanding detection in real time, improves the efficiency of work, and uses folder stream method carry out the sample introduction of sample and divide in the detection
From, improve the automaticity of analysis process, improve work efficiency；Avoid sample transfer between different devices, letter
Change manual operation.
Accompanying drawing explanation
Fig. 1 is this utility model structural representation,
Fig. 2 is this utility model chip structure schematic diagram,
Wherein, 1, photomultiplier tube, 2, optical filter one, 3, optical filter two, 4, diaphragm, 5, battery of lens, 6, two to color mirror, 7,
Object lens, 8, chip, 9, beam expander, 10, optical filter three, 11, semiconductor laser, 12, sample cell, 13, buffer pool, 14, sample
Product give up pond liquid, 15, buffer give up pond, 16, split tunnel, 17, power supply.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of this utility model rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under making creative work premise
The every other embodiment obtained, broadly falls into the scope of this utility model protection.
Referring to Fig. 1-2, this utility model provides a kind of technical scheme: a kind of micro-fluidic chip realizing PCR and in real time
The bacteria detecting apparatus of PCR, described detection device includes that photomultiplier tube 1, described photomultiplier tube 1 are internally provided with optical filter
One 2 are disposed with diaphragm 4, battery of lens 5 and object lens 6, described battery of lens 5 with optical filter 23, described photomultiplier tube 1 bottom
And being provided with two between object lens 7 and be provided with semiconductor laser 11 on the right side of color mirror 6, described detection device, described quasiconductor swashs
Being provided with beam expander 9 and optical filter 3 10 between light device 11 and detection device, described detection bottom of device is provided with chip 8.
Be provided with split tunnel 16 on described chip 8, described split tunnel 16 both sides be respectively arranged with buffer pool 13 with
Buffer gives up pond 15, and described split tunnel 16 upper and lower sides is respectively arranged with sample and gives up pond liquid 14 and sample cell 12.
The position of described semiconductor laser 11 with two between color mirror 6 mutually the most corresponding.
Described buffer pool 13, buffer give up pond liquid 14, the sample cell 12 of pond 15, sample that give up all connects power supply by platinum electrode
17。
Described chip 8 is the micro-fluidic chip of PCR.
In sum, this utility model uses semiconductor laser 11 as excitation source, reflects into thing through dichroic mirror 6
Mirror 7, after being focused on by object lens 7 in the split tunnel 16 of vertical incidence chip, thus inspires fluorescence, improves the efficiency of detection,
Fluorescence signal is by monitor record, the information of understanding detection that can be real-time after photomultiplier tube is collected simultaneously, improves work
Efficiency, and use folder stream method to carry out the sample introduction of sample and separate in the detection, improve the automaticity of analysis process, carry
High workload efficiency；Avoid sample transfer between different devices, simplify manual operation.
Embodiment the most of the present utility model, for the ordinary skill in the art,
It is appreciated that in the case of without departing from principle of the present utility model and spirit and these embodiments can be carried out multiple change, repair
Changing, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. the micro-fluidic chip realizing PCR and the bacteria detecting apparatus of real-time PCR, it is characterised in that: described detection device
Including photomultiplier tube (1), described photomultiplier tube (1) is internally provided with optical filter one (2) and optical filter two (3), described light
Electricity multiplier tube (1) bottom is disposed with diaphragm (4), battery of lens (5) and object lens (6), described battery of lens (5) and object lens (7) it
Between be provided with two on the right side of color mirror (6), described detection device, be provided with semiconductor laser (11), described semiconductor laser
(11) and being provided with beam expander (9) and optical filter three (10) between detection device, described detection bottom of device is provided with chip
(8)。
The bacteria detecting apparatus of a kind of micro-fluidic chip realizing PCR the most according to claim 1 and in real time PCR, it is special
Levy and be: be provided with split tunnel (16) on described chip (8), described split tunnel (16) both sides are respectively arranged with buffer pool
(13) giving up pond (15) with buffer, described split tunnel (16) upper and lower sides is respectively arranged with sample and gives up pond liquid (14) and sample cell
(12)。
The bacteria detecting apparatus of a kind of micro-fluidic chip realizing PCR the most according to claim 1 and in real time PCR, it is special
Levy and be: the position of described semiconductor laser (11) with two between color mirror (6) mutually the most corresponding.
The bacteria detecting apparatus of a kind of micro-fluidic chip realizing PCR the most according to claim 2 and in real time PCR, it is special
Levy and be: described buffer pool (13), buffer give up pond liquid (14), the sample cell (12) of pond (15), sample that give up all passes through platinum electrode
Connect power supply (17).
The bacteria detecting apparatus of a kind of micro-fluidic chip realizing PCR the most according to claim 1 and in real time PCR, it is special
Levy and be: described chip (8) is the micro-fluidic chip of PCR.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620742561.3U CN205774545U (en) | 2016-07-14 | 2016-07-14 | A kind of bacteria detecting apparatus of the micro-fluidic chip realizing PCR and in real time PCR |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620742561.3U CN205774545U (en) | 2016-07-14 | 2016-07-14 | A kind of bacteria detecting apparatus of the micro-fluidic chip realizing PCR and in real time PCR |
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| CN205774545U true CN205774545U (en) | 2016-12-07 |
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| CN201620742561.3U Active CN205774545U (en) | 2016-07-14 | 2016-07-14 | A kind of bacteria detecting apparatus of the micro-fluidic chip realizing PCR and in real time PCR |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106978338A (en) * | 2017-05-24 | 2017-07-25 | 济南市疾病预防控制中心 | A kind of device of the micro-fluidic integrated detection of automatic bacteria |
-
2016
- 2016-07-14 CN CN201620742561.3U patent/CN205774545U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106978338A (en) * | 2017-05-24 | 2017-07-25 | 济南市疾病预防控制中心 | A kind of device of the micro-fluidic integrated detection of automatic bacteria |
| CN106978338B (en) * | 2017-05-24 | 2023-06-27 | 济南市疾病预防控制中心 | Automatic integrated device that detects of bacterial micro-fluidic |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191211 Address after: 100176, No. 22, Tongji North Road, Beijing economic and Technological Development Zone, Beijing, Daxing District Patentee after: Beijing Zhifei Lvzhu Biopharmaceutical Co., Ltd. Address before: 238000 Anhui city of Hefei province Chaohu City Juchao District Laoshan Road Hefei Career Technical College Patentee before: Hefei Vocationl Technical College |
|
| TR01 | Transfer of patent right |