CN202256254U - Computer microscopy cell electrophoresis system - Google Patents

Computer microscopy cell electrophoresis system Download PDF

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Publication number
CN202256254U
CN202256254U CN2011203697531U CN201120369753U CN202256254U CN 202256254 U CN202256254 U CN 202256254U CN 2011203697531 U CN2011203697531 U CN 2011203697531U CN 201120369753 U CN201120369753 U CN 201120369753U CN 202256254 U CN202256254 U CN 202256254U
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China
Prior art keywords
computing machine
phase contrast
contrast microscope
viewer
computer
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Expired - Fee Related
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CN2011203697531U
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Chinese (zh)
Inventor
孙朝阳
陈德喜
翟庆华
候进振
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CHAOYANG BIOTECHNOLOGY Co Ltd
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CHAOYANG BIOTECHNOLOGY Co Ltd
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Priority to CN2011203697531U priority Critical patent/CN202256254U/en
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Abstract

The utility model belongs to a computer microscopy cell electrophoresis system. A system power supply provides needed power supply for the computer microscopy cell electrophoresis system and connected with a phase contrast microscope and a computer, the phase contrast microscope reads information about moving direction and speed of particles spread in liquid phase media of a microscopy electrophoresis observer in an electric field and sends the information to the computer, and the phase contrast microscope is connected with the computer. The computer processes and outputs the information read from the phase contrast microscope and is connected with the phase contrast microscope. The computer is connected with an input system, a printer and a display screen. The computer processes and outputs the information read from the phase contrast microscope, accuracy of data observation, calculation and recording of observed objects is greatly improved, and the computer microscopy cell electrophoresis system has the advantages of being short in experiment time, reasonable in structural design and simple and convenient to operate, saving time and labor, and being good in using effects.

Description

Computing machine microscopic cells electrophoresis system
Technical field
The utility model belongs to a kind of computing machine microscopic cells electrophoresis system.
Background technology
Electrophoresis apparatus is widely used in pharmacy, medical science, food, weight industry, controls in the fields such as gold, oil.Electrophoresis apparatus be separate being used to of using always, the instrument of identification of dna, RNA molecule mixture, this electrophoresis apparatus as holder, utilizes charge effect and the molecular sieving effect of DVA molecule when swimming with agar gel, reaches the purpose of separating mixture.Dna molecular is electronegative in being higher than the solution of its isoelectric point, and anode moves in electric field.Under certain electric field intensity, the migration velocity of dna molecular depends on molecular sieving effect, and promptly the size of molecule itself and configuration are main influence factors.Electrophoresis apparatus is divided into level and vertical stratification, and the electrophoresis apparatus of horizontal structure is put the offset plate horizontal positioned, and the electrophoresis apparatus of vertical stratification is put offset plate and vertically placed; But both communicate by the glue groove with the glue groove usually and are positioned at the slot electrode at its two ends, and the positive and negative electrode line that is positioned at slot electrode forms, and the positive and negative electrode line of the electrophoresis apparatus of this structure communicates with external positive-negative power through the both positive and negative polarity socket aperture; Examine with observing, calculating and record; Complicated operation, observation, calculating and record data poor accuracy, speed of experiment is slow; Structural design is unreasonable, and result of use is poor.
Summary of the invention
The purpose of the utility model is a kind of computing machine microscopic cells electrophoresis system of design; Also export the information that reads from phase contrast microscope with Computer Processing; Increase substantially the accuracy of observation, calculating and the record data of measured object, have speed of experiment fast, reasonable in design; Easy and simple to handle, save labor and the good advantage of result of use.For this reason, the utility model mainly is made up of computing machine, input system, printer and display screen, comprises system power supply, and for computing machine microscopic cells electrophoresis system provides required power supply, system power supply connects phase contrast microscope device and computing machine; Phase contrast microscope reads the information of the particulate that is scattered in the microelectrophoresis viewer in liquid phase medium moving direction and the speed in electric field and inputs to computing machine, and phase contrast microscope connects computing machine; Computing machine, the information that processing and output are read from phase contrast microscope, computing machine is connected with phase contrast microscope; Computing machine connects input system, printer and display screen.Described phase contrast microscope is put platform, mistake filter, microlens, camera, falsework, up-down adjustment button, left and right adjusting button and base by lamp house, adjusting camera lens, viewer and is constituted; Base is provided with falsework, and falsework one end is provided with lamp house, is provided with the adjusting camera lens before the light exit of lamp house; Viewer is put platform and is positioned at adjusting camera lens front end; Viewer is put on the falsework of platform front end and was established filter, and the falsework other end is provided with microlens, and the microlens front end was positioned at the filter rear side; Camera is established in the microlens rear end, establishes the video socket that is connected with computing machine on the camera.Described falsework is L-shaped.Described up-down adjustment button, left and right adjusting button are positioned at the falsework middle part.Described microelectrophoresis viewer is to establish sample observation ward at transparent body middle part; Sample observation ward both upper ends thereof is provided with sample perfusing hole and exhausr port; Two electrode chambers lay respectively on the body in perfusing hole and the exhausr port outside, and two electrode chamber lower ends communicate with sample observation ward lower end through isolating glue-line.Described microelectrophoresis viewer is put at viewer and is put on the platform.The said structure design has reached the purpose of the utility model.
The information that the utility model reads from phase contrast microscope with Computer Processing and output; Increase substantially the accuracy of observation, calculating and the record data of measured object, had speed of experiment fast, reasonable in design; Easy and simple to handle, save labor and the good advantage of result of use.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Fig. 2 is the phase contrast microscope structural representation of the utility model.
Fig. 3 is the microelectrophoresis viewer structural representation of the utility model.
Specific embodiments
To shown in Figure 3, a kind of computing machine microscopic cells electrophoresis system mainly is made up of computing machine 3, input system 5, printer 4 and 6 of display screens like Fig. 1.Input system is keyboard and mouse.Computing machine comprises the video frequency collection card that can read the phase contrast microscope video information; And the system software of phase contrast microscope video information is read, handles and calculates in processing; And the information that will read, handle and the calculate printer and the display screen that show constantly and print.The utility model comprises:
System power supply 1 for computing machine microscopic cells electrophoresis system provides required power supply, contains transformer and voltage stabilizer, and output voltage is 10~150V, and burning voltage.System power supply connects phase contrast microscope device and computing machine;
Phase contrast microscope 2 reads the information of the particulate that is scattered in the microelectrophoresis viewer in liquid phase medium moving direction and the speed in electric field and inputs to computing machine, and phase contrast microscope connects computing machine;
Computing machine 3, the information that processing and output are read from phase contrast microscope, computing machine is connected with phase contrast microscope.Computing machine connects input system, printer and display screen.
Described phase contrast microscope is put platform 23, mistake filter 24, microlens 25, camera 26, falsework 28, up-down adjustment button 20, left and right adjusting button 210 and 29 of bases by lamp house 21, adjusting camera lens 22, viewer and is constituted.Base is provided with falsework, and falsework one end is provided with lamp house, is provided with the adjusting camera lens before the light exit of lamp house; Viewer is put platform and is positioned at adjusting camera lens front end; Viewer is put on the falsework of platform front end and was established filter, and the falsework other end is provided with microlens, and the microlens front end was positioned at the filter rear side; Camera is established in the microlens rear end, establishes the video socket 27 that is connected with computing machine on the camera.Described falsework is L-shaped.Described up-down adjustment button, left and right adjusting button are positioned at falsework middle part, and up-down adjustment button, left and right adjusting button are regulated viewer respectively and put the platform position that moves up and down.
Described microelectrophoresis viewer is to establish sample observation ward 12 at transparent body 11 middle parts, and sample observation ward both upper ends thereof is provided with sample perfusing hole 8 and lays respectively on the body in the perfusing hole and the exhausr port outside with exhausr port 9, two electrode chambers 7,10.Two electrode chamber lower ends communicate with sample observation ward lower end through isolating glue-line 13.Described microelectrophoresis viewer is put at viewer and is put on the platform.
During use; The utility model system power supply and AC power are connected; System power supply is for each provides required stabilized power source with electric device, the cell of gathering inserted in the sample observation ward and this microelectrophoresis viewer put at viewer from the sample perfusing hole of microelectrophoresis viewer after with diluted put platform, and the positive and negative polarities attaching plug is inserted two electrode chambers; Utilize charge effect and the molecular sieving effect of cellular elements when swimming, reach the purpose of separating mixture.Cellular elements is electronegative in being higher than the solution of its isoelectric point, and anode moves in electric field.Under certain electric field intensity, the migration velocity of cellular elements depends on molecular sieving effect.Phase contrast microscope evenly illuminates sample observation indoor sample by the light of lamp house after regulating camera lens; Cross filter and the sample light source information filtration that is fit to is reached computing machine by the microlens picked-up through camera again, the information that Computer Processing and output are read from phase contrast microscope.
In a word, the utility model is with Computer Processing and export the information that reads from phase contrast microscope, has increased substantially the accuracy of observation, calculating and the record data of measured object; There is speed of experiment fast; Reasonable in design, easy and simple to handle, save labor and the good advantage of result of use.

Claims (6)

1. a computing machine microscopic cells electrophoresis system mainly is made up of computing machine, input system, printer and display screen, it is characterized in that:
System power supply, for computing machine microscopic cells electrophoresis system provides required power supply, system power supply connects phase contrast microscope device and computing machine;
Phase contrast microscope reads the information of the particulate that is scattered in the microelectrophoresis viewer in liquid phase medium moving direction and the speed in electric field and inputs to computing machine, and phase contrast microscope connects computing machine;
Computing machine, the information that processing and output are read from phase contrast microscope, computing machine is connected with phase contrast microscope; Computing machine connects input system, printer and display screen.
2. by the described computing machine microscopic cells of claim 1 electrophoresis system; It is characterized in that: described phase contrast microscope is put platform, mistake filter, microlens, camera, falsework, up-down adjustment button, left and right adjusting button and base by lamp house, adjusting camera lens, viewer and is constituted; Base is provided with falsework, and falsework one end is provided with lamp house, is provided with the adjusting camera lens before the light exit of lamp house; Viewer is put platform and is positioned at adjusting camera lens front end; Viewer is put on the falsework of platform front end and was established filter, and the falsework other end is provided with microlens, and the microlens front end was positioned at the filter rear side; Camera is established in the microlens rear end, establishes the video socket that is connected with computing machine on the camera.
3. by the described computing machine microscopic cells of claim 2 electrophoresis system, it is characterized in that: described falsework is L-shaped.
4. by the described computing machine microscopic cells of claim 2 electrophoresis system, it is characterized in that: described up-down adjustment button, left and right adjusting button are positioned at the falsework middle part.
5. by the described computing machine microscopic cells of claim 1 electrophoresis system; It is characterized in that: described microelectrophoresis viewer is to establish sample observation ward at transparent body middle part; Sample observation ward both upper ends thereof is provided with sample perfusing hole and exhausr port; Two electrode chambers lay respectively on the body in perfusing hole and the exhausr port outside, and two electrode chamber lower ends communicate with sample observation ward lower end through isolating glue-line.
6. by the described computing machine microscopic cells of claim 5 electrophoresis system, it is characterized in that: described microelectrophoresis viewer is put at viewer and is put on the platform.
CN2011203697531U 2011-10-08 2011-10-08 Computer microscopy cell electrophoresis system Expired - Fee Related CN202256254U (en)

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CN2011203697531U CN202256254U (en) 2011-10-08 2011-10-08 Computer microscopy cell electrophoresis system

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CN2011203697531U CN202256254U (en) 2011-10-08 2011-10-08 Computer microscopy cell electrophoresis system

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402236A (en) * 2018-11-12 2019-03-01 长江大学 The detection method of blast resistant gene Pi-25 in a kind of rice breed

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402236A (en) * 2018-11-12 2019-03-01 长江大学 The detection method of blast resistant gene Pi-25 in a kind of rice breed

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Granted publication date: 20120530

Termination date: 20131008