CN203249894U - Real-time visual nucleic acid electrophoresis device - Google Patents
Real-time visual nucleic acid electrophoresis device Download PDFInfo
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- CN203249894U CN203249894U CN 201320136988 CN201320136988U CN203249894U CN 203249894 U CN203249894 U CN 203249894U CN 201320136988 CN201320136988 CN 201320136988 CN 201320136988 U CN201320136988 U CN 201320136988U CN 203249894 U CN203249894 U CN 203249894U
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Abstract
The utility model relates a real-time visual nucleic acid electrophoresis device which comprises a light source component and an electrophoresis tank, wherein the electrophoresis tank is arranged above the light source component; at least two groups of visible light sources with wavelengths in a specific range are uniformly arranged on the light source component; the electrophoresis tank comprises a bottom tank and an upper cover arranged above the bottom tank; the bottom tank comprises a fully transparent and colorless bottom surface; the upper cover comprises a light filter plate which only allows rays with wavelengths in a specific range to pass through; and the visible light sources, the bottom surface of the bottom tank and the light filter plates are arranged correspondingly up and down. The real-time visual nucleic acid electrophoresis device saves experiment time, improves the experiment efficiency, greatly simplifies a fluorescent nucleic acid electrophoresis device, and reduces the fluorescent nucleic acid electrophoresis cost.
Description
Technical field
The utility model relates to a kind of nucleic acid electrophoresis appts, relates in particular a kind of Real time visible nucleic acid electrophoresis appts that reuses, and belongs to the molecular biology experiment field.
Background technology
The gel electrophoresis of DNA and RNA is one of normal experiment in biological study and field of medicaments always, no matter be the checking of in plasmid, extracting, PCR result's displaying, or gene order-checking early stage sample preliminary work recruitment evaluation, the gel electrophoresis of nucleic acid all is the most direct effective method that the information such as nucleic acid purity, concentration, clip size are provided for the experimenter.Along with the development of biological medicine technology, for ease for use and the wide usage of DNA and RNA gel electrophoresis more and more higher demand has been proposed.
Now widely used nucleic acid developing technique mainly based on ultraviolet source for being embedded in exciting of pyridine of bromination (EB) in the sample of nucleic acid, observe the position of nucleic acid swimming in gel and the purpose that reclaims nucleic acid.This technology mainly contains following defective: (1) EB is high intoxicating material, has very strong mutagenesis, cause that easily cell generation canceration and high volatility are difficult to again degraded, this requires to use the laboratory of EB to set up special EB contaminated area, the experimenter who carries out simultaneously nucleic acid electrophoresis and experiment need carry out very careful protection, otherwise will produce harm to experimenter even whole laboratory; (2) ultraviolet source possesses the harmfulness of dna damage equally, easily the experimenter is produced damage; (3) EB develops needs the specialized equipment of ultraviolet imagery, and not only price is higher, and electrophoresis process is separated with developing process, has reduced the controllability of electrophoresis experiment, has wasted the experimental period of a lot of preciousnesses.
In recent years, countries in the world are all in the possibility of being devoted to study the EB substitute, such as genefinder, and the appearance of the fluorescent core acid dyes such as sypro green.These fluorescent core acid dyes adopt visible light source fluorescence excitation nucleic acid dye to produce the visible detection light source under the prerequisite that guarantees sensitivity, reach the purpose that detects nucleic acid.The appearance of these fluorescent core acid dyes has proposed active demand to the research and development take nontoxic low toxicity fluorescent core acid dye as the fluorescence nucleic acid electrophoresis equipment on basis.At present, existing product has greatly limited its application on the market owing to can not reuse.
Given this, the utility model takes full advantage of the characteristic of nontoxic low toxicity fluorescent core acid dye, discloses a kind of movable reusable fluorescence nucleic acid electrophoresis appts, is used for Real Time Observation nucleic acid in the position of gel swimming and reclaims nucleic acid; Characteristics of the present utility model be greatly abbreviation the fluorescence nucleic acid electrophoresis appts, reduced fluorescence nucleic acid electrophoresis cost, filled up the home market blank, be conducive to promote biology and medical science, the development in the fields such as drug design.
The utility model content
Technical problem to be solved in the utility model is to overcome the shortcoming of prior art, the Real time visible nucleic acid electrophoresis appts that provide a kind of simplified structure, reduction cost and Protection personal security, can reuse.
In order to solve above technical matters, the utility model provides a kind of Real time visible nucleic acid electrophoresis appts, comprises light source assembly and electrophoresis tank, and described electrophoresis tank is arranged at described light source assembly top; The visible light source of evenly distributed at least two group wavelength in particular range on the described light source assembly; Described electrophoresis tank comprises kerve and is arranged at the loam cake of described kerve top, described kerve comprises the bottom surface that all-transparent is colourless, described loam cake comprises the filter that the light that only allows the particular range wavelength passes through, and the bottom surface of described light source assembly (1), described kerve and described filter be corresponding the setting up and down.
The technical scheme that the utility model further limits is: the wavelength particular range of described visible light source and described filter allow the wavelength coverage of the light that passes through without common factor.
Further, the wavelength coverage of described visible light source is 488nm～510nm, and the wavelength coverage of the light that described filter permission is passed through is 510nm-520nm.
Further, the visible light source of described wavelength in particular range is led light source.
Further, in the described kerve carbon electrode is set.
Further, described light source assembly, described kerve and described loam cake are rectangle.
Further, also comprise base, described base is the two stage steps shape, and the first order ledge surface of described base arranges a groove, and described light source assembly embeds described inside grooves, the upper surface of described light source assembly and the upper surface flush of described base.
The beneficial effects of the utility model are: a kind of Real time visible nucleic acid electrophoresis appts described in the utility model utilizes visible light source to excite the specific nucleic acid dyestuff to produce the principle of specific wavelength detection light source and the characteristic of nontoxic low toxicity fluorescent core acid dye, the position of Real Time Observation nucleic acid swimming in gel and recovery nucleic acid, saved test period, heightened conventional efficient, greatly abbreviation the fluorescence nucleic acid electrophoresis appts, reduced fluorescence nucleic acid electrophoresis cost, filled up the home market blank, be conducive to promote biology and medical science, the development in the fields such as drug design.
Description of drawings
Fig. 1 is the structural representation of Real time visible nucleic acid electrophoresis appts described in the utility model;
Fig. 2 is the structural representation of electrophoresis tank described in the utility model;
Fig. 3 is the structural representation of base described in the utility model.
Embodiment
A kind of Real time visible nucleic acid electrophoresis appts that the present embodiment provides, its structural representation comprise base 7, light source assembly 1 and electrophoresis tank 2 as shown in Figure 1.
The structural representation of base 7 is the two stage steps shape as shown in Figure 3, for device provides support and power interface.Base 7 links to each other with power supply by wire, and at the second level step operating switch and electric field switch is set, and can connect as required or cut off the electricity supply and control the power on/off of electric field; And, at the facade place of two stage steps electric field is set and connects the hole.The first order ledge surface of base 7 arranges a rectangular recess, and the size of groove is big or small corresponding with light source assembly 1.
The bottom surface of led light source, kerve 3 and filter be corresponding the setting about in the of 5, the bottom surface of the light transmission kerve 3 that led light source sends shines electrophoresis tank 2 inside, excitation line excites the coloring agent that is incorporated on the nucleic acid molecules, produce the detection light of specific wavelength, detect light and see through filter 5 and catch for human eye or other optical instruments (such as camera etc.), thereby demonstrate the positions of nucleic acid fragment in gel of different sizes.
Real time visible nucleic acid electrophoresis appts described in the utility model in the course of the work, on request fluorescent core acid dye (such as sypro green etc.) is added in the gel of fusing when the preparation gel, with solidifying in the kerve 3 that Ago-Gel is placed on electrophoresis tank 2 of preparing, add a small amount of electrophoresis liquid, cover loam cake 4.Electrophoresis tank 2 is placed on the top of the light source assembly 1 that is embedded on the base 7, the switch of engaging means and electric field switch.Under the effect of stationary electric field, nucleic acid can displacement, the simultaneously exciting light of the led light source generation fixed wave length under the kerve.Nucleic acid material presents the ionic condition of the negative some property of band in electrophoretic buffer, can be in Ago-Gel under the effect of stationary electric field by negative electrode to the positive electrode direction swimming, nucleic acid material speed of swimming in gel that nucleotide quantity is more is slower, the nucleic acid molecules of different fragments size is just separated in the process of electrophoresis, in swimming, be positioned at the excitation line (blue bent arrow) of the led light source generation specific wavelength under the kerve, excitation line excites the coloring agent that is incorporated on the nucleic acid molecules, produce the detection light of specific wavelength, detecting light, to see through optical filter be that human eye or other optical instruments (such as camera etc.) are caught, thereby demonstrate the positions of nucleic acid fragment in gel of different sizes.
Compare with product of the same type both at home and abroad, loam cake 4 and the kerve 3 of electrophoresis tank 2 of the present utility model are split-type design, can make things convenient for the experimenter after electrophoresis finishes, to cut the band of the nucleic acid molecules of wanting, simultaneously, electrophoresis process and nucleic acid develop and carry out simultaneously, can be according to different experiments requirement, at any time stop electrophoresis, greatly save test period, heightened conventional efficient.
In addition to the implementation, the utility model can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of the utility model requirement.
Claims (7)
1. a Real time visible nucleic acid electrophoresis appts is characterized in that, comprises light source assembly (1) and electrophoresis tank (2), and described electrophoresis tank (2) is arranged at described light source assembly (1) top; The upper visible light source of evenly distributed at least two group wavelength in particular range of described light source assembly (1); Described electrophoresis tank (2) comprises kerve (3) and is arranged at the loam cake (4) of described kerve (3) top, described kerve (3) comprises the bottom surface that all-transparent is colourless, described loam cake (4) comprises the filter (5) that the light that only allows the particular range wavelength passes through, and the bottom surface of described light source assembly (1), described kerve (3) and described filter (5) be corresponding the setting up and down.
2. a kind of Real time visible nucleic acid electrophoresis appts according to claim 1 is characterized in that, the wavelength particular range of described visible light source and described filter (5) allow the wavelength coverage of the light that passes through without common factor.
3. a kind of Real time visible nucleic acid electrophoresis appts according to claim 2 is characterized in that, the wavelength coverage of described visible light source is 488nm～510nm, and the wavelength coverage of the light that described filter (5) permission is passed through is 510nm-520nm.
4. a kind of Real time visible nucleic acid electrophoresis appts according to claim 1 is characterized in that, the visible light source of described wavelength in particular range is led light source.
5. a kind of Real time visible nucleic acid electrophoresis appts according to claim 1 is characterized in that, carbon electrode (6) is set in the described kerve (3).
6. a kind of Real time visible nucleic acid electrophoresis appts according to claim 1 is characterized in that, described light source assembly (1), described kerve (3) and described loam cake (4) are rectangle.
7. the described a kind of Real time visible nucleic acid electrophoresis appts of arbitrary claim according to claim 1-5, it is characterized in that, also comprise base (7), described base (7) is the two stage steps shape, the first order ledge surface of described base (7) arranges a groove, described light source assembly (1) embeds described inside grooves, the upper surface flush of the upper surface of described light source assembly (1) and described base (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320136988 CN203249894U (en) | 2013-03-25 | 2013-03-25 | Real-time visual nucleic acid electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320136988 CN203249894U (en) | 2013-03-25 | 2013-03-25 | Real-time visual nucleic acid electrophoresis device |
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| Publication Number | Publication Date |
|---|---|
| CN203249894U true CN203249894U (en) | 2013-10-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320136988 Expired - Lifetime CN203249894U (en) | 2013-03-25 | 2013-03-25 | Real-time visual nucleic acid electrophoresis device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064497A (en) * | 2017-06-13 | 2017-08-18 | 福建省农业科学院畜牧兽医研究所 | A kind of portable electrophoresis and photographing device and its application method |
| CN107340327A (en) * | 2017-07-24 | 2017-11-10 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
-
2013
- 2013-03-25 CN CN 201320136988 patent/CN203249894U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064497A (en) * | 2017-06-13 | 2017-08-18 | 福建省农业科学院畜牧兽医研究所 | A kind of portable electrophoresis and photographing device and its application method |
| CN107340327A (en) * | 2017-07-24 | 2017-11-10 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131023 |
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| CX01 | Expiry of patent term |