CN107340327A - A kind of gel electrophoresis groove with LED light source - Google Patents
A kind of gel electrophoresis groove with LED light source Download PDFInfo
- Publication number
- CN107340327A CN107340327A CN201710604710.9A CN201710604710A CN107340327A CN 107340327 A CN107340327 A CN 107340327A CN 201710604710 A CN201710604710 A CN 201710604710A CN 107340327 A CN107340327 A CN 107340327A
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- Prior art keywords
- led
- light source
- groove
- gel electrophoresis
- electrophoresis
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000001502 gel electrophoresis Methods 0.000 title claims abstract description 20
- 238000001962 electrophoresis Methods 0.000 claims abstract description 26
- 201000009310 astigmatism Diseases 0.000 claims abstract description 22
- 210000005056 cell body Anatomy 0.000 claims abstract description 18
- 239000012780 transparent material Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 description 5
- 230000005684 electric field Effects 0.000 description 5
- 230000005012 migration Effects 0.000 description 4
- 238000013508 migration Methods 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229920000447 polyanionic polymer Polymers 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/453—Cells therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to a kind of gel electrophoresis groove with LED light source, including cell body, electrophoresis platform, groove lid, electrode, LED, astigmatism plate, astigmatism plate upper strata is provided with filtered region with LED junction.Electrode is symmetrical arranged.LED and astigmatism plate are detachable connection.LED is realized by electric wire with power supply and electrically connects or be powered by internal battery.LED is from belt switch.Cell body, groove lid are transparent material.The present invention is by setting transparent cell body, groove lid, so as to be observed in real time to the experimental conditions of gel electrophoresis, meanwhile, by setting LED at the top of groove lid, and light source scattering angle is controlled by astigmatism plate, all standing in electrophoresis region can be realized by limited light source.In addition, setting switch in LED, when that need not observe, LED can be closed, save the energy, electrophoresis progress will not be impacted again.
Description
Technical field
The present invention relates to field of portable devices, more precisely, being the gel electrophoresis groove with LED light source.
Background technology
The principle of gel electrophoresis is fairly simple.When a kind of molecule is placed among electric field, they will be with certain
Speed shifts to appropriate electrode, migration velocity of this electrophoresis molecule under electric field action, is called the mobility of electrophoresis.It is the same as electricity
Intensity and electrophoresis molecule entrained net charges are directly proportional in itself.That is, electric-field intensity is bigger, electrophoresis molecule institute
The net charge quantity of carrying is more, and the speed that it is migrated is also faster, on the contrary then slower.Due to having used a kind of nothing in electrophoresis
The supporting dielectric of the stabilization of reactivity, such as Ago-Gel and polyacrylamide gel, so as to reduce convective motion, thus it is electric
The mobility of swimming is that the coefficient of friction of same molecule is inversely proportional again.Known coefficient of friction is that bulk of molecule, polarity and medium glue
The function of degree, therefore according to the number of the difference of the difference of molecular size, composition or shape, and the net charge of institute's band,
With by electrophoresis by the various composition in protein or nucleic acid molecules mixture it is separated from each other come.In physiological conditions, nucleic acid
Phosphate group in sugar-phosphate backbones of molecule is in ionic condition, and from this sense, DNA and RNA polynucleotide chains can be named
Do polyanion (Polyanions).Therefore, when nucleic acid molecules are placed in the electric field, they will be to the side of positive electrode
To migration.Due to the repetitive nature in sugar-phosphate backbones structure, the double-stranded DNA of identical quantity almost has the net charge of equivalent,
Thus they can be migrated to positive electrode direction at a same speed.Under certain electric-field intensity, this migration speed of DNA molecular
Degree, that is, the mobility of electrophoresis, depending on nucleic acid molecules size in itself and configuration, the less DNA molecular of molecular weight compare molecule
It is quicker to measure larger DNA molecular migration.
Under normal circumstances, it is required for observing inside special facilities for observation after the completion of gel electrophoresis, there is presently no one
Kind can carry out the gel electrophoresis groove with LED light source of real-time monitored during electrophoresis progress.
The content of the invention
The present invention mainly solves the technical problem present in prior art, can be carried out so as to provide one kind in electrophoresis
The gel electrophoresis groove with LED light source of real-time monitored is carried out in journey.
The above-mentioned technical problem of the present invention is mainly what is be addressed by following technical proposals：
Gel electrophoresis groove with LED light source, including cell body, electrophoresis platform, groove lid, electrode, LED, astigmatism plate, its feature is in groove
Body is rectangular parallelepiped structure, and cell body bottom centre position forms electrophoresis platform towards upper process, and electrophoresis platform both sides are provided with electrode, groove
Groove lid is provided with the top of body, top of electrodes is higher by groove lid, and astigmatism plate is provided with the middle part of groove lid, LED is provided with the top of astigmatism plate.
Astigmatism plate upper strata is provided with filtered region with LED junction.Electrode is symmetrical arranged.LED and astigmatism plate are detachable company
Connect.LED is realized by electric wire with power supply and electrically connects or be powered by internal battery.LED is from belt switch.Cell body, groove
Cover as transparent material.
The present invention is by setting transparent cell body, groove lid, so as to be seen in real time to the experimental conditions of gel electrophoresis
Survey, meanwhile, light source scattering angle is controlled by setting LED at the top of groove lid, and by astigmatism plate, limited light can be passed through
Realize all standing in electrophoresis region in source.In addition, setting switch in LED, when that need not observe, LED can be closed, saved
The about energy, electrophoresis progress will not be impacted again.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the overall structure diagram of the present invention, wherein：
1-cell body, 2-electrophoresis platform, 3-groove lid, 4-electrode, 5-LED, 6-astigmatism plate.
Embodiment
The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, so that advantages and features of the invention energy
It is easier to be readily appreciated by one skilled in the art, apparent is clearly defined so as to be made to protection scope of the present invention.
Gel electrophoresis groove with LED light source, including cell body 1, electrophoresis platform 2, groove lid 3, electrode 4, LED 5, astigmatism plate 6,
Its feature is rectangular parallelepiped structure in cell body 1, and the bottom centre position of cell body 1 forms electrophoresis platform 2,2 liang of electrophoresis platform towards upper process
Side is provided with electrode 4, and the top of cell body 1 is provided with groove lid 3, and the top of electrode 4 is higher by groove lid 3, and the middle part of groove lid 3 is provided with astigmatism plate 6,
The top of astigmatism plate 6 is provided with LED 5.The upper strata of astigmatism plate 6 is provided with filtered region with the junction of LED 5.Electrode 4 is symmetrically set
Put.LED 5 and astigmatism plate 6 are detachable connection.LED 5 is realized by electric wire with power supply and electrically connects or pass through internal battery
It is powered.LED 5 is from belt switch.Cell body 1, groove lid 3 are transparent material.
The present invention is by setting transparent cell body 1, groove lid 3, so as to be carried out in real time to the experimental conditions of gel electrophoresis
Observation, meanwhile, by setting LED 5 at the top of groove lid 3, and light source scattering angle is controlled by astigmatism plate 6, can be by having
The light source of limit realizes all standing in electrophoresis region.In addition, switch is set in LED 5, can be by LED when that need not observe
5 close, and save the energy, electrophoresis progress will not be impacted again.
This is not limited to, any change or replacement expected without creative work, should all cover the guarantor in the present invention
Within the scope of shield.Therefore, protection scope of the present invention should be determined by the scope of protection defined in the claims.
Claims (7)
1. a kind of gel electrophoresis groove with LED light source, including cell body, electrophoresis platform, groove lid, electrode, LED, astigmatism plate, it is special
It is rectangular parallelepiped structure to levy in cell body, and cell body bottom centre position forms electrophoresis platform towards upper process, and electrophoresis platform both sides are provided with
Electrode, cell body top are provided with groove lid, and top of electrodes is higher by groove lid, and astigmatism plate is provided with the middle part of groove lid, is set at the top of astigmatism plate
There is LED.
A kind of 2. gel electrophoresis groove with LED light source according to claim 1, it is characterised in that the astigmatism plate upper strata with
LED junction is provided with filtered region.
3. a kind of gel electrophoresis groove with LED light source according to claim 1, it is characterised in that the electrode is symmetrically set
Put.
4. a kind of gel electrophoresis groove with LED light source according to claim 1, it is characterised in that the LED and astigmatism
Plate is detachable connection.
5. a kind of gel electrophoresis groove with LED light source according to claim 4, it is characterised in that the LED and power supply
Electrical connection is realized by electric wire or is powered by internal battery.
6. a kind of gel electrophoresis groove with LED light source according to claim 4, it is characterised in that the LED carries out
Close.
7. a kind of gel electrophoresis groove with LED light source according to claim 1, it is characterised in that the cell body, groove lid are
Transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710604710.9A CN107340327A (en) | 2017-07-24 | 2017-07-24 | A kind of gel electrophoresis groove with LED light source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710604710.9A CN107340327A (en) | 2017-07-24 | 2017-07-24 | A kind of gel electrophoresis groove with LED light source |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107340327A true CN107340327A (en) | 2017-11-10 |
Family
ID=60216348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710604710.9A Pending CN107340327A (en) | 2017-07-24 | 2017-07-24 | A kind of gel electrophoresis groove with LED light source |
Country Status (1)
| Country | Link |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201477073U (en) * | 2009-05-07 | 2010-05-19 | 程浩然 | Agarose gel DNA/RNA electrophoresis apparatus |
| CN101949886A (en) * | 2010-08-27 | 2011-01-19 | 亚亚科技股份有限公司 | Real-time fluorescence electrophoresis apparatus |
| TWM406181U (en) * | 2011-01-10 | 2011-06-21 | Major Science Co Ltd | Lighted base applicable for electrophoresis tank |
| CN203249894U (en) * | 2013-03-25 | 2013-10-23 | 南京山诺生物科技有限公司 | Real-time visual nucleic acid electrophoresis device |
| CN203422341U (en) * | 2013-07-26 | 2014-02-05 | 常州市肿瘤医院 | Device capable of gel electrophoresis observation in real time |
| CN205026524U (en) * | 2015-08-31 | 2016-02-10 | 江苏顺仕净化设备有限公司 | Easily clean LED lamps and lanterns of ceiling type |
| CN205538812U (en) * | 2016-04-23 | 2016-08-31 | 朱书礼 | Agarose gel electrophoresis groove |
| CN207163967U (en) * | 2017-07-24 | 2018-03-30 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
-
2017
- 2017-07-24 CN CN201710604710.9A patent/CN107340327A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201477073U (en) * | 2009-05-07 | 2010-05-19 | 程浩然 | Agarose gel DNA/RNA electrophoresis apparatus |
| CN101949886A (en) * | 2010-08-27 | 2011-01-19 | 亚亚科技股份有限公司 | Real-time fluorescence electrophoresis apparatus |
| TWM406181U (en) * | 2011-01-10 | 2011-06-21 | Major Science Co Ltd | Lighted base applicable for electrophoresis tank |
| CN203249894U (en) * | 2013-03-25 | 2013-10-23 | 南京山诺生物科技有限公司 | Real-time visual nucleic acid electrophoresis device |
| CN203422341U (en) * | 2013-07-26 | 2014-02-05 | 常州市肿瘤医院 | Device capable of gel electrophoresis observation in real time |
| CN205026524U (en) * | 2015-08-31 | 2016-02-10 | 江苏顺仕净化设备有限公司 | Easily clean LED lamps and lanterns of ceiling type |
| CN205538812U (en) * | 2016-04-23 | 2016-08-31 | 朱书礼 | Agarose gel electrophoresis groove |
| CN207163967U (en) * | 2017-07-24 | 2018-03-30 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| WD01 | Invention patent application deemed withdrawn after publication | ||
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Application publication date: 20171110 |